DETEKSI DISTRIBUSI WHITE SPOT SYNDROME VIRUS PADA BERBAGAI ORGAN UDANG VANAME (Litopenaeus vannamei)

One way to detect the presence of the White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the method of Polymerase Chain Reaction (PCR). WSSV virus isknown to attack various target organs, but it is difficult to detect early on shrimp. Therefore, this study aims todetect the presence of the distribution of White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the Polymerase Chain Reaction method in order to early detectionefforts for the prevention and treatment of diseases caused by the virus. Organ observed that the swimming legs,gills, stomach and antennal gland. Research stages include sample preparation and DNA extraction, DNAamplification and electrophoresis. DNA extraction was performed using the method DTAB and CTAB-DNAamplification is done by using nested PCR. The emergence of DNA bands is an indicator of the presence ofWSSV in shrimp Vaname several organs which are then presented desktriptif. The results showed four WSSVinfected organs were observed. Based on the appearance, the entire sample of the swimming leg and antennalgland positively infected, then successively gills and stomach. The swimming leg can be used to diagnose thedisease early on shrimp WSSV Vaname without turning off the shrimp.Keywords : Distribution, Vanname shrimp, WSSV, PC

Deteksi Distribusi White Spot Syndrome Virus pada Berbagai Organ Udang Vaname (Litopenaeus Vannamei)

One way to detect the presence of the White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the method of Polymerase Chain Reaction (PCR). WSSV virus isknown to attack various target organs, but it is difficult to detect early on shrimp. Therefore, this study aims todetect the presence of the distribution of White Spot Syndrome Virus in several organs Vaname shrimp(Litopenaeus vannamei) were infected with the Polymerase Chain Reaction method in order to early detectionefforts for the prevention and treatment of diseases caused by the virus. Organ observed that the swimming legs,gills, stomach and antennal gland. Research stages include sample preparation and DNA extraction, DNAamplification and electrophoresis. DNA extraction was performed using the method DTAB and CTAB-DNAamplification is done by using nested PCR. The emergence of DNA bands is an indicator of the presence ofWSSV in shrimp Vaname several organs which are then presented desktriptif. The results showed four WSSVinfected organs were observed. Based on the appearance, the entire sample of the swimming leg and antennalgland positively infected, then successively gills and stomach. The swimming leg can be used to diagnose thedisease early on shrimp WSSV Vaname without turning off the shrimp

Support For The Comprehensive School Health Model To Increase Reproductive Health Knowledge Of Elementary School Children As A Primary Prevention Effort

The research explores the impact of the Comprehensive School Health model on enhancing the reproductive health knowledge of primary school children as a primary prevention effort. In an era of rapid social and technological changes, early-age reproductive health awareness is crucial. This study aims to evaluate the effectiveness of the Comprehensive School Health model in improving the reproductive health knowledge of primary school children. The purpose of this research is to assess whether the Comprehensive School Health model significantly enhances the reproductive health knowledge of primary school children. It seeks to determine if this approach can be a viable strategy for primary prevention in the field of reproductive health. The study employs a pre-experimental design with a One Group Pretest-Posttest Design. Data was collected through observations, interviews, and documentation. The sample consists of sixth-grade students from an elementary school in Sorong District, selected using total sampling. Statistical analysis included Wilcoxon tests and rank biserial correlation. The results indicate a significant improvement in the reproductive health knowledge of primary school children following the intervention. The Wilcoxon test showed a p-value of <0.001, and the mean difference was -35.0, signifying a substantial increase in knowledge. The effect size was 1.62, indicating a significant impact, and a strong negative rank biserial correlation of -1.00 revealed a robust correlation between the intervention and knowledge improvement. This study concludes that the Comprehensive School Health model is effective in significantly enhancing the reproductive health knowledge of primary school children, with a substantial effect size and a strong correlation between the intervention and knowledge improvement. These findings emphasize the importance of early-age reproductive health education and highlight the potential of this model as an effective strategy for primary prevention in the field of reproductive health

Observations from the NILU-UV Antarctic network since 2000

Póster elaborado para la SCAR Open Science Conference celebrada en Auckland, Nueva Zelanda los días 25-28 de agosto de 2014Total ozone and UV measurements have been performed with the NILU-UV radiometer at the station of Ushuaia (54◦S), Marambio (64◦S) and Belgrano II (77◦S) since 2000. The network was established in 1999/2000 by the Spanish Agencia Estatal de Meteorología (AEMET) in collaboration with the innishMeteorological Institute (FMI), the Argentinian Dirección Nacional del Antártico-Instituto Antártico (DNA-IAA) and Centro Austral de Investigaciones Cientificas (CADIC). The location of the network was chosen in order to monitor total ozone and UV radiation at different sides of the polar vortex: Belgrano II is mostly located inside the vortex, Marambio at various times inside, on the edge of, or outside the vortex, while Ushuaia is mostly outside the vortex.The MAR Project was financed by the National R+D Plan of the Spanish Ministry of Science and Technology (National Research Program in the Antarctic) under contract REN2000-0245-C02-01

The four-wavelength Photoacoustic Aerosol Absorption Spectrometer (PAAS-4<i>λ</i>)

In this paper, the Photoacoustic Aerosol Absorption Spectrometer (PAAS-4λ) is introduced. PAAS-4λ was specifically developed for long-term monitoring tasks in (unattended) air quality stations. It uses four wavelengths coupled to a single acoustic resonator in a compact and robust set-up. The instrument has been thoroughly characterized and carefully calibrated in the laboratory using NO2/air mixtures and Nigrosin aerosol. It has an ultimate 1σ detection limit below 0.1 Mm−1, at a measurement precision and accuracy of 3 % and 10 %, respectively. In order to demonstrate the PAAS-4λ suitability for long-term monitoring tasks, the instrument is currently validated at the air quality monitoring station Pallas in Finland, about 140 km north of the Arctic circle. A total of 11 months of PAAS-4λ data from this deployment are presented and discussed in terms of instrument performance. Intercomparisons with the filter-based photometers of a continuous soot monitoring system (COSMOS), the Multi-Angle Absorption Photometer (MAAP), and Aethalometer (AE33) demonstrate the capabilities and value of PAAS-4λ, as well as for the validation of the widely used filter-based instruments.</p

The Science of Marine Protected Areas (3rd edition, Mediterranean)

The main purpose of the booklet is to present the latest scientific information about the effects of MPAs in the Mediterranean in order to inform current management dialogues. This is particularly relevant given the increasing legislative frameworks and political initiatives to implement networks of MPAs in countries across the Mediterranean Sea. Importantly, this Edition does much more than simply tailor the earlier content for the Mediterranean region. The edition update the basic content of the booklet, drawing on the wealth of new published scientific literature, highlighting case studies from the Mediterranean Sea

Intercomparison and evaluation of global aerosol microphysical properties among AeroCom models of a range of complexity

Many of the next generation of global climate models will include aerosol schemes which explicitly simulate the microphysical processes that determine the particle size distribution. These models enable aerosol optical properties and cloud condensation nuclei (CCN) concentrations to be determined by fundamental aerosol processes, which should lead to a more physically based simulation of aerosol direct and indirect radiative forcings. This study examines the global variation in particle size distribution simulated by 12 global aerosol microphysics models to quantify model diversity and to identify any common biases against observations. Evaluation against size distribution measurements from a new European network of aerosol supersites shows that the mean model agrees quite well with the observations at many sites on the annual mean, but there are some seasonal biases common to many sites. In particular, at many of these European sites, the accumulation mode number concentration is biased low during winter and Aitken mode concentrations tend to be overestimated in winter and underestimated in summer. At high northern latitudes, the models strongly underpredict Aitken and accumulation particle concentrations compared to the measurements, consistent with previous studies that have highlighted the poor performance of global aerosol models in the Arctic. In the marine boundary layer, the models capture the observed meridional variation in the size distribution, which is dominated by the Aitken mode at high latitudes, with an increasing concentration of accumulation particles with decreasing latitude. Considering vertical profiles, the models reproduce the observed peak in total particle concentrations in the upper troposphere due to new particle formation, although modelled peak concentrations tend to be biased high over Europe. Overall, the multi-model-mean data set simulates the global variation of the particle size distribution with a good degree of skill, suggesting that most of the individual global aerosol microphysics models are performing well, although the large model diversity indicates that some models are in poor agreement with the observations. Further work is required to better constrain size-resolved primary and secondary particle number sources, and an improved understanding of nucleation and growth (e.g. the role of nitrate and secondary organics) will improve the fidelity of simulated particle size distributions

Overview: Recent advances in the understanding of the northern Eurasian environments and of the urban air quality in China – a Pan-Eurasian Experiment (PEEX) programme perspective

The Pan-Eurasian Experiment (PEEX) Science Plan, released in 2015, addressed a need for a holistic system understanding and outlined the most urgent research needs for the rapidly changing Arctic-boreal region. Air quality in China, together with the long-range transport of atmospheric pollutants, was also indicated as one of the most crucial topics of the research agenda. These two geographical regions, the northern Eurasian Arctic-boreal region and China, especially the megacities in China, were identified as a “PEEX region”. It is also important to recognize that the PEEX geographical region is an area where science-based policy actions would have significant impacts on the global climate. This paper summarizes results obtained during the last 5 years in the northern Eurasian region, together with recent observations of the air quality in the urban environments in China, in the context of the PEEX programme. The main regions of interest are the Russian Arctic, northern Eurasian boreal forests (Siberia) and peatlands, and the megacities in China. We frame our analysis against research themes introduced in the PEEX Science Plan in 2015. We summarize recent progress towards an enhanced holistic understanding of the land–atmosphere–ocean systems feedbacks. We conclude that although the scientific knowledge in these regions has increased, the new results are in many cases insufficient, and there are still gaps in our understanding of large-scale climate–Earth surface interactions and feedbacks. This arises from limitations in research infrastructures, especially the lack of coordinated, continuous and comprehensive in situ observations of the study region as well as integrative data analyses, hindering a comprehensive system analysis. The fast-changing environment and ecosystem changes driven by climate change, socio-economic activities like the China Silk Road Initiative, and the global trends like urbanization further complicate such analyses. We recognize new topics with an increasing importance in the near future, especially “the enhancing biological sequestration capacity of greenhouse gases into forests and soils to mitigate climate change” and the “socio-economic development to tackle air quality issues”

Comparative effectiveness of autologous hematopoietic stem cell transplant vs fingolimod, natalizumab, and ocrelizumab in highly active relapsing-remitting multiple sclerosis

Importance: Autologous hematopoietic stem cell transplant (AHSCT) is available for treatment of highly active multiple sclerosis (MS). Objective: To compare the effectiveness of AHSCT vs fingolimod, natalizumab, and ocrelizumab in relapsing-remitting MS by emulating pairwise trials. Design, Setting, and Participants: This comparative treatment effectiveness study included 6 specialist MS centers with AHSCT programs and international MSBase registry between 2006 and 2021. The study included patients with relapsing-remitting MS treated with AHSCT, fingolimod, natalizumab, or ocrelizumab with 2 or more years study follow-up including 2 or more disability assessments. Patients were matched on a propensity score derived from clinical and demographic characteristics. Exposure: AHSCT vs fingolimod, natalizumab, or ocrelizumab. Main outcomes: Pairwise-censored groups were compared on annualized relapse rates (ARR) and freedom from relapses and 6-month confirmed Expanded Disability Status Scale (EDSS) score worsening and improvement. Results: Of 4915 individuals, 167 were treated with AHSCT; 2558, fingolimod; 1490, natalizumab; and 700, ocrelizumab. The prematch AHSCT cohort was younger and with greater disability than the fingolimod, natalizumab, and ocrelizumab cohorts; the matched groups were closely aligned. The proportion of women ranged from 65% to 70%, and the mean (SD) age ranged from 35.3 (9.4) to 37.1 (10.6) years. The mean (SD) disease duration ranged from 7.9 (5.6) to 8.7 (5.4) years, EDSS score ranged from 3.5 (1.6) to 3.9 (1.9), and frequency of relapses ranged from 0.77 (0.94) to 0.86 (0.89) in the preceding year. Compared with the fingolimod group (769 [30.0%]), AHSCT (144 [86.2%]) was associated with fewer relapses (ARR: mean [SD], 0.09 [0.30] vs 0.20 [0.44]), similar risk of disability worsening (hazard ratio [HR], 1.70; 95% CI, 0.91-3.17), and higher chance of disability improvement (HR, 2.70; 95% CI, 1.71-4.26) over 5 years. Compared with natalizumab (730 [49.0%]), AHSCT (146 [87.4%]) was associated with marginally lower ARR (mean [SD], 0.08 [0.31] vs 0.10 [0.34]), similar risk of disability worsening (HR, 1.06; 95% CI, 0.54-2.09), and higher chance of disability improvement (HR, 2.68; 95% CI, 1.72-4.18) over 5 years. AHSCT (110 [65.9%]) and ocrelizumab (343 [49.0%]) were associated with similar ARR (mean [SD], 0.09 [0.34] vs 0.06 [0.32]), disability worsening (HR, 1.77; 95% CI, 0.61-5.08), and disability improvement (HR, 1.37; 95% CI, 0.66-2.82) over 3 years. AHSCT-related mortality occurred in 1 of 159 patients (0.6%). Conclusion: In this study, the association of AHSCT with preventing relapses and facilitating recovery from disability was considerably superior to fingolimod and marginally superior to natalizumab. This study did not find evidence for difference in the effectiveness of AHSCT and ocrelizumab over a shorter available follow-up time