The effect of administration order of BU and CY on toxicity in hematopoietic SCT in humans

Universidade Federal de São Paulo, Div Bone Marrow Transplantat, São Paulo, BrazilUniversidade Federal de São Paulo, Div Bone Marrow Transplantat, São Paulo, BrazilWeb of Scienc

Growth of melon cultivated under saline stress and potassium doses

O Estado do Rio Grande do Norte, em destaque a região da Chapada do Apodi, se destaca na produção e exportação de melão no País, em regime de irrigação, devido à distribuição pluvial baixa e irregular. A Chapada do Apodi possui dois aqüíferos subterrâneos; a do lençol menos profundo de alta salinidade, porém com menor custo de bombeamento, ocorrendo o contrário com o de maior profundidade. Objetivou-se, ante o exposto, avaliar o efeito de duas águas de salinidades diferentes (0,52 e 2,41 dS m-1), combinadas com cinco doses de K2O (218, 273, 328, 383 e 438 kg ha-1) sobre o crescimento do meloeiro (Cucumis melo L.), cultivar Goldex, utilizando-se do delineamento de blocos ao acaso, com parcelas subdivididas; amostras de planta foram coletadas aos 21, 28, 35, 49 e 63 dias após a semeadura, determinando-se a fitomassa seca das plantas, estas separadas em ramos (caule + folhas), flores e frutos; avaliou-se, também, a taxa de crescimento absoluto e relativo e a produção de frutos. Em geral, o crescimento do melão foi favorecido com o uso de água mais salina; a taxa de crescimento absoluto foi máxima entre 35 e 49 dias após a semeadura. Obteve-se maior produção de fitomassa total com 438 kg ha-1 de K2O e uso de água mais salina, ao final do ciclo.The region of 'Chapada do Apodi', in the State of Rio Grande do Norte, stands out in Brazilian production and exportation of melon. This region possesses two aquiffers, one of lower exploration cost, though of high salinity, another of low salinity, with higher cost and limited use. This work was carried out with the objective to evaluate the effect of waters of different salinities combined with five doses of K in the dry matter accumulation and productivity of the melon (Cucumis melo L.) cultivar Goldex. The experimental design adopted was split plot in completely randomized blocks. The melon crop was irrigated with low (0.52 dS m-1) and high (2.41 dS m-1) salinity water combined with five doses of K2O (218, 273, 328, 383 and 438 kg ha-1). Plants were collected at 21, 28, 35, 49 and 63 days after sowing, and were separated in branches (shoot + leaves), flowers and fruits, and the total dry matter of the aerial parts obtained by summation. The absolute and relative growth rates and the production of fruits were also evaluated. The absolute growth rate of the plant was maximum between 35 and 49 days. The highest total biomass was obtained with 438 kg ha-1 K2O and use of high salinity water at the end of crop cycle.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq

Proposal of a virtual collaborative news environment: an interdisciplinary study

The main objective of this work is to demonstrate the advances made by the CATI research group with regard to the proposal to create a virtual collaborative news environment (AVNC) , to be initially implemented at the Northern Fluminense Darcy Ribeiro State University(UENF) through the Postgraduate Program in Cognition and Language. This interdisciplinary environment involves concepts from the area of communication, administration and information technology and seeks to meet the demands of generation, storage, retrieval, processing and transmission of information in this digital age. The AVNC involves three research fronts: rethinking the logic of news production, structuring a platform that is collaborative and defining what a business and management model should be that can give sustainability to this environment. In today\u27s world, it is important to consider the reader\u27s increasingly active participation in journalism, through information and communication technologies, thanks to the social changes that come with them. In times of cyberspace and cyberculture, it is necessary to rethink the dynamics of journalistic production. For this, authors like Pierre Lévy, Lúcia Santaella, Henry Jenkins, Caio Túlio Costa, among others were searched. In an era in which forms of collaborative ownership prevail, the platform being considered in this research follows the 3C collaboration model, according to Michalsky, Mamani, and Gerosa. A global platform where individuals interact, communicate, collaborate and gather information requires a business model and management that assists in managing the collaborative virtual news environment. For this, we used authors such as Alex Osterwalder, Eric Flamholtz, Siqueira and Crispim, Campos, among others. With this, an increasingly intelligent and collaborative environment is expected, with the active participation of all the agents involved. That is, what is proposed is the total interaction of the Internet user-reader

Relationship between pulmonary function and physical activity in daily life in patients with COPD

SummaryBackgroundIt remains unclear how closely the physical inactivity observed in patients with Chronic Obstructive Pulmonary Disease (COPD) relates to the severity of their airflow limitation. Furthermore, it is unknown whether spirometric variables such as maximal voluntary ventilation (MVV) and inspiratory capacity (IC) reflect the level of physical activity in daily life better than the forced expiratory volume in the first second (FEV1), the main spirometric variable used to determine the severity of COPD. The objective of the present study was to investigate the relationship between physical activity in daily life and the severity of COPD assessed by different spirometric variables: MVV, IC and FEV1.MethodsForty patients with COPD (21 men; 68±7 years; FEV1 41±14% predicted) were performed spirometry and assessment of the physical activity level in daily life using an accelerometer (SenseWear® Armband).ResultsMVV was significantly correlated to total energy expenditure per day, energy expenditure per day in activities demanding more than 3 metabolic equivalents (METs), number of steps per day and time spent per day in moderate and vigorous activities (0.42≤r≤0.52; p<0.01 for all). Correlation of these variables with IC and especially FEV1 was more modest, borderline or not statistically significant. There was no difference in time spent in vigorous activities among patients classified according to the FEV1-based GOLD stages II, III and IV, differently than that observed when patients were classified in groups according to their MVV.ConclusionIn COPD patients, MVV better reflects the physical activity level in daily life than FEV1 and IC

A self-organizing map clustering approach to support territorial zoning

This work aims to evaluate three strategies for analyzing clusters of ordinal categorical data (thematic maps) to support the territorial zoning of the Alto Taquari basin, MS/MT. We evaluated a model-based method, another based on the segmentation of the multi-way contingency table, and the last one based on the transformation of ordinal data into intervals and subsequent analysis of clusters from a proposed method of segmentation of the Self-Organizing Map after the neural network training process. The results showed the adequacy of the methods based on the Self-Organizen Map and the segmentation of the contingency table, as these techniques generated unimodal clusters with distinguishable groups.Supported by National Council for Scientific and Technological Development –CNPq, Brazil, and by National Funds through the Portuguese funding agency, FCT -Fundação para a Ciência e a Tecnologia within project 2022.06822.PTDC. The work of Pedro Oliveira was also supported by the doctoral Grant PRT/BD/154311/2022 financed by the Portuguese Foundation for Science and Technology (FCT), and with funds from European Union, under MIT Portugal Progra

Rectosigmoidectomy in a patient with a prolapsed sigmoid tumor: case report

Modelo de estudo: Relato de caso. Objetivo do estudo: Relatar o caso de uma paciente com prolapso de tumor colorretal localizado no sigmóide. Metodologia: Relato de caso obtido de prontuário. Resultados: É relatado o caso de uma paciente que aguardava cirurgia para ressecção oncológica de um tumor colorretal, quando foi encaminhada ao serviço de urgências médicas com episódio de prolapso retal contendo massa tumoral pós-evacuação, sem redução espontânea. A paciente foi encaminhada à cirurgia de urgência e submetida a uma abordagem transabdominal, na qual o prolapso foi reduzido, em seguida realizada retossigmoidectomia segundo procedimento de Hartamann com colostomia terminal no cólon descendente. Conclusões: No caso relatado, por tratar-se de um paciente portador de tumor de sigmóide prolapsado, a abordagem de escolha foi a retosigmoidectomia abdominal, seguindo os preceitos oncológicos, com ligadura da artéria mesentérica inferior em sua origem. Importância do problema: O prolapso de tumor de sigmóide, uma situação clínica relativamente incomum na população adulta, e uma complicação mais rara quando se trata dos tumores colorretais, deve ser tratado imediatamente após o diagnóstico para se diminuir o risco de isquemia do segmento prolapsado e sempre que possível oferecer a cirurgia que siga os preceitos oncológicos de tratamento. Comentários: No caso relatado, foi necessário uma abordagem cirúrgica de urgência, sendo a via abdominal escolhida por respeito aos princípios da cirurgia oncológica e ao menor tempo cirúrgicoDesign of the study: Case report. Objectives: To report a case of a patient with colorectal tumor prolapse located in the sigmoid. Methodology: A retrospective case report obtained from medical records. Results: We report a case of a patient awaiting surgery for tumor resection of a colorectal tumor, when she was sent to the service of medical emergencies with an episode of post-evacuation rectal prolapse with a tumor without spontaneous reduction. The patient was referred to emergency surgery and underwent a transabdominal approach, in which the prolapse was reduced then followed by Hartmann's rectosigmodectomy surgical procedure. Conclusions: Sigmoid tumor prolapse require a surgical approach, the approach of choice was abdominal retosigmoidectomy, following the oncological principles, with ligation of the inferior mesenteric artery at its origin. Relevance: Sigmoid tumor prolapse, a relatively unusual clinical situation in adults, and more rare complication when it comes to colorectal tumors, it must be treated immediately after diagnosis to reduce the risk of ischemia prolapsed segment, and whenever possible offer surgery to follow the precepts of cancer treatment. Comments: In the case reported, an emergency surgical approach was necessary, and the abdominal route was chosen following the principles of cancer surgery and shorter surgical tim

Multicenter randomized trial of cell therapy in cardiopathies – MiHeart Study

BACKGROUND: Cardiovascular diseases are the major cause of death in the world. Current treatments have not been able to reverse this scenario, creating the need for the development of new therapies. Cell therapies have emerged as an alternative for cardiac diseases of distinct causes in experimental animal studies and more recently in clinical trials. METHOD/DESIGN: We have designed clinical trials to test for the efficacy of autologous bone marrow derived mononuclear cell therapies in four different cardiopathies: acute and chronic ischemic heart disease, and Chagasic and dilated cardiomyopathy. All trials are multicenter, randomized, double-blind and placebo controlled. In each trial 300 patients will be enrolled and receive optimized therapy for their specific condition. Additionally, half of the patients will receive the autologous bone marrow cells while the other half will receive placebo (saline with 5% autologous serum). For each trial there are specific inclusion and exclusion criteria and the method for cell delivery is intramyocardial for the chronic ischemic heart disease and intracoronary for all others. Primary endpoint for all studies will be the difference in ejection fraction (determined by Simpson's rule) six and twelve months after intervention in relation to the basal ejection fraction. The main hypothesis of this study is that the patients who receive the autologous bone-marrow stem cell implant will have after a 6 month follow-up a mean increase of 5% in absolute left ventricular ejection fraction in comparison with the control group. DISCUSSION: Many phase I clinical trials using cell therapy for cardiac diseases have already been performed. The few randomized studies have yielded conflicting results, rendering necessary larger well controlled trials to test for efficacy of cell therapies in cardiopathies. The trials registration numbers at the NIH registry are the following: Chagasic cardiomyopathy (NCT00349271), dilated cardiomyopathy (NCT00333827), acute myocardial infarction (NCT00350766) and Chronic Ischemic Heart Disease (NCT00362388)

Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula

We studied the fungal diversity present in soils sampled along a deglaciated chronosequence from para- to periglacial conditions on James Ross Island, north-east Antarctic Peninsula, using DNA metabarcoding. A total of 88 amplicon sequence variants (ASVs) were detected, dominated by the phyla Ascomycota, Basidiomycota and Mortierellomycota. The uncommon phyla Chytridiomycota, Rozellomycota, Monoblepharomycota, Zoopagomycota and Basidiobolomycota were detected. Unknown fungi identified at higher hierarchical taxonomic levels (Fungal sp. 1, Fungal sp. 2, Spizellomycetales sp. and Rozellomycotina sp.) and taxa identified at generic and specific levels (Mortierella sp., Pseudogymnoascus sp., Mortierella alpina, M. turficola, Neoascochyta paspali, Penicillium sp. and Betamyces sp.) dominated the assemblages. In general, the assemblages displayed high diversity and richness, and moderate dominance. Only 12 of the fungal ASVs were detected in all chronosequence soils sampled. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected. Based on the sequence diversity obtained, Clearwater Mesa soils contain a complex fungal community, including the presence of fungal groups generally considered rare in Antarctica, with dominant taxa recognized as cold-adapted cosmopolitan, endemic, saprotrophic and phytopathogenic fungi. Clearwater Mesa ecosystems are impacted by the effects of regional climatic changes, and may provide a natural observatory to understand climate change effects over time

High connectivity of the Crocodile Shark between the Atlantic and Southwest Indian Oceans: highlights for conservation

Among the various shark species that are captured as bycatch in commercial fishing operations, the group of pelagic sharks is still one of the least studied and known. Within those, the crocodile shark, Pseudocarcharias kamoharai, a small-sized lamnid shark, is occasionally caught by longline vessels in certain regions of the tropical oceans worldwide. However, the population dynamics of this species, as well as the impact of fishing mortality on its stocks, are still unknown, with the crocodile shark currently one of the least studied of all pelagic sharks. Given this, the present study aimed to assess the population structure of P. kamoharai in several regions of the Atlantic and Indian Oceans using genetic molecular markers. The nucleotide composition of the mitochondrial DNA control region of 255 individuals was analyzed, and 31 haplotypes were found, with an estimated diversity Hd = 0.627, and a nucleotide diversity pi = 0.00167. An analysis of molecular variance (AMOVA) revealed a fixation index phi(ST) = -0.01118, representing an absence of population structure among the sampled regions of the Atlantic Ocean, and between the Atlantic and Indian Oceans. These results show a high degree of gene flow between the studied areas, with a single genetic stock and reduced population variability. In panmictic populations, conservation efforts can be concentrated in more restricted areas, being these representative of the total biodiversity of the species. When necessary, this strategy could be applied to the genetic maintenance of P. kamoharai.Foundation for Research Support of the Sao Paulo State - FAPESP [2011/23787-0, 2010/51903-2]; Portuguese Foundation for Science and Technology (FCT) [SFRH/BPD/93936/2013]; Foundation for Research Support of the Sao Paulo State - FAPESP [2011/23787-0, 2010/51903-2]; Portuguese Foundation for Science and Technology (FCT) [SFRH/BPD/93936/2013]info:eu-repo/semantics/publishedVersio