XRCC2 R188H (rs3218536), XRCC3 T241M (rs861539) and R243H (rs77381814) single nucleotide polymorphisms in cervical cancer risk

Human Papillomavirus (HPV) is the main cause of cervical cancer and its precursor lesions. Transformation may be induced by several mechanisms, including oncogene activation and genome instability. Individual differences in DNA damage recognition and repair have been hypothesized to influence cervical cancer risk. The aim of this study was to evaluate whether the double strand break gene polymorphisms XRCC2 R188H G>A (rs3218536), XRCC3 T241M C>T (rs861539) and R243H G>A (rs77381814) are associated to cervical cancer in Argentine women. A case control study consisting of 322 samples (205 cases and 117 controls) was carried out. HPV DNA detection was performed by PCR and genotyping of positive samples by EIA (enzyme immunoassay). XRCC2 and 3 polymorphisms were determined by pyrosequencing. The HPV-adjusted odds ratio (OR) of XRCC2 188 GG/AG genotypes was OR = 2.4 (CI = 1.1-4.9, p = 0.02) for cervical cancer. In contrast, there was no increased risk for cervical cancer with XRCC3 241 TT/CC genotypes (OR = 0.48; CI = 0.2-1; p = 0.1) or XRCC3 241 CT/CC (OR = 0.87; CI = 0.52-1.4; p = 0.6). Regarding XRCC3 R243H, the G allele was almost fixed in the population studied. In conclusion, although the sample size was modest, the present data indicate a statistical association between cervical cancer and XRCC2 R188H polymorphism. Future studies are needed to confirm these findings.Fil: Perez, Luis Orlando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Crivaro, Andrea Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Barbisan, Gisela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Poleri, Lucía Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Golijow, Carlos Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentin

ANALISIS TINGKAT KEMATANGAN GONAD IKAN GABUS (Channa striata) YANG DIPACU DENGAN PENYUNTIKAN GONADOTROPIN RELEASING HORMONE DAN ANTI DOPAMINE (GnRH-a) DOSIS BERBEDA

Pemberian hormonal dari luar sangat membantu terhadap keberhasilan pemijahan, karena dapat mempercepat proses perkembangan gonad. Penelitian ini bertujuan untuk mengetahui pengaruh peyuntikan Gonadotropin Releasing Hormone (GnRH) dan anti dopamine dosis berbeda terhadap Tingkat kematangan gonad (TKG), Gonado Somatik Indeks (GSI), dan Hepato Somatik Indeks (HSI) ikan gabus (Channa striata). Penelitian ini dilakukan selama 9 jam pada bulan November Di Laboratorium Basah Perikanan Perikanan, Jurusan Perikanan, Fakultas Pertanian, Universitas Palangka Raya. Rancangan percobaan yang digunakan yaitu rancangan acak lengkap (RAL) 4 perlakuan 3 ulangan. Perlakuan A tanpa menggunakan hormon (kontrol), perlakuan B dosis 0,3 ml, perlakuan C 0,5 ml, dan perlakuan D dosis 0,7 ml untuk 1 kg ikan gabus. Hasil penelitian ini menunjukkan bahwa Tingkat kematangan gonad (TKG) tertinggi terdapat pada perlakuan D dengan dosis 0,7 ml/kg yaitu TKG V. Nilai Gonado Somatik Indeks (GSI) tertinggi didapat pada perlakuan D dosis 0,7 ml/kg yaitu 6,20% Nilai Hepato Somatik Indeks (HSI) tertinggi terdapat pada perlakuan B dosis 0,3 ml/kg yaitu 1.07%. Oleh karena itu, penelitian ini menunjukkan bahwa penyuntikan Gonadotropin Releasing Hormone (GnRH) dan anti dopamine berpengaruh terhadap Tingkat kematangan gonad (TKG), Gonado Somatik Indeks (GSI), dan Hepato Somatik Indeks (HSI) ikan gabus (Channa striata)

Time to revise the paradigm of hantavirus syndromes? Hantavirus pulmonary syndrome caused by European hantavirus

Hantaviruses have previously been recognised to cause two separate syndromes: hemorrhagic fever with renal syndrome in Eurasia, and hantavirus pulmonary syndrome (HPS) in the Americas. However, increasing evidence suggests that this dichotomy is no longer fruitful when recognising human hantavirus disease and understanding the pathogenesis. Herein are presented three cases of severe European Puumala hantavirus infection that meet the HPS case definition. The clinical and pathological findings were similar to those found in American hantavirus patients. Consequently, hantavirus infection should be considered as a cause of acute respiratory distress in all endemic areas worldwide

Forested Wetlands of the Southern United States: A Bibliography

The term forested wetland covers a variety of forest types including mangroves, cypress/tupelo swamps, bottomland hardwoods, pocosins and Carolina bays, flatwoods, and mountain fens. These forests are dominated by woody species that have morphological features, physiological adaptations, and/or reproductive strategies enabling them to achieve maturity and reproduce in an environment where the soils within the rooting zone may be inundated or saturated for various periods during the growing season. Although alluvial floodplains occur along most streams of the United States, they are most extensive in the Atlantic Coastal Plain, Gulf Coastal Plain, and Mississippi Alluvial Plain. Only about half of the original floodplain forests remained by the 1930s, and conversion to agriculture continued at an accelerated pace during the 1960s and 1970s.The purpose of this bibliography is to provide a detailed listing of references for students and researchers of the varied studies conducted in these forest types

Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya

Several alphaviruses, such as chikungunya (CHIKV) and Onyong-nyong (ONNV), are endemic in Kenya and often cause outbreaks in different parts of the country. We assessed the seroprevalence of alphaviruses in patients with acute febrile illness in two geographically distant areas in Kenya with no previous record of alphavirus outbreaks. Blood samples were collected from febrile patients in health facilities located in the rural Taita-Taveta County in 2016 and urban Kibera informal settlement in Nairobi in 2017 and tested for CHIKV IgG and IgM antibodies using an in-house immunofluorescence assay (IFA) and a commercial ELISA test, respectively. A subset of CHIKV IgG or IgM antibody-positive samples were further analyzed using plaque reduction neutralization tests (PRNT) for CHIKV, ONNV, and Sindbis virus. Out of 537 patients, 4 (0.7%) and 28 (5.2%) had alphavirus IgM and IgG antibodies, respectively, confirmed on PRNT. We show evidence of previous and current exposure to alphaviruses based on serological testing in areas with no recorded history of outbreaks.Peer reviewe

Serological Evidence of Exposure to Onyong-Nyong and Chikungunya Viruses in Febrile Patients of Rural Taita-Taveta County and Urban Kibera Informal Settlement in Nairobi, Kenya

Several alphaviruses, such as chikungunya (CHIKV) and Onyong-nyong (ONNV), are endemic in Kenya and often cause outbreaks in different parts of the country. We assessed the seroprevalence of alphaviruses in patients with acute febrile illness in two geographically distant areas in Kenya with no previous record of alphavirus outbreaks. Blood samples were collected from febrile patients in health facilities located in the rural Taita-Taveta County in 2016 and urban Kibera informal settlement in Nairobi in 2017 and tested for CHIKV IgG and IgM antibodies using an in-house immunofluorescence assay (IFA) and a commercial ELISA test, respectively. A subset of CHIKV IgG or IgM antibody-positive samples were further analyzed using plaque reduction neutralization tests (PRNT) for CHIKV, ONNV, and Sindbis virus. Out of 537 patients, 4 (0.7%) and 28 (5.2%) had alphavirus IgM and IgG antibodies, respectively, confirmed on PRNT. We show evidence of previous and current exposure to alphaviruses based on serological testing in areas with no recorded history of outbreaks.Peer reviewe

PEMANFAATAN PEKARANGAN MELALUI BUDIDAYA TANAMAN SAYURAN MENGGUNAKAN MEDIA POLYBAG SEBAGAI UPAYA OPTIMALISASI LAHAN

Kuliah Kerja Nyata (KKN) Tematik Mandiri Universitas Palangka Raya Tahun 2022. Penempatan di Desa Penda Pilang, Kecamatan Kurun, Kabupaten Gunung Mas, Provinsi Kalimantan Tengah. Penerapan budidaya inovasi pertanian berperan dalam meningkatkan produktivitas usaha tani, kelompok ibu-ibu Pemberdayaan dan Kesejahteraan Keluarga (PKK) di Desa Penda Pilang, sehingga berpeluang untuk meningkatkan kesejahteraan hidup, yang salah satunya diindikasikan dari meningkatnya ketahanan pangan rumah tangga. Penelitian ini bertujuan untuk mengindentifikasi inovasi kelembagaan pertanian yang telah diterapkan di Desa Penda Pilang, serta menganalisis hubungannya dengan kondisi ketahanan pangan pada rumah tangga. Metode pemanfaatan pekarangan budidaya sayuran berupa melaksanakan penanaman bibit-bibit bahan pangan yang mana ditanam di lahan pertanian ibu-ibu PKK di Desa Penda Pilang. Adapun tujuan lain dari pelaksanaan program kerja pemanfaatan tersebut yaitu mengaktifkan kembali daya tarik semangat ibu-ibu PKK di bidang ketahanan pangan di Desa Penda Pilang. Dengan hadirnya mahasiswa sebagai agen perubahan mencoba memberikan sentuhan perubahan ke arah kemajuan dengan berbagai gagasan dan inovasi yang teraktualisasi dalam setiap program kerja yang dilaksanakan bersama dengan warga masyarakat Desa Penda Pilang. Salah satunya dengan melakukan penguatan kelembagaan petani kepada ibu-ibu Pemberdayaan dan Kesejahteraan Keluarga dalam mensejahterakan hidup, serta meningkatkan semangat masyarakat dalam ketahanan pangan di Desa Penda Pilan

Caracterização fisiológica do crescimento da planta de batata-doce

This research was installed to study plant growth of sweet potato and identify the most favorable harvest time for both roots and the aboveground part (stems). The experiment was carried out at the JK campus of the Federal University of the Vale do Jequitinhonha e Mucuri, in Diamantina, MG, complete blocks at random, with four replications. Three plants in the central part of the experimental plots were collected in twelve consecutive harvests at 15-day intervals. Plants were fractionated in roots, stems, and leaves to determine the dry matter of each part. Leaf area was also measured and growth rates were calculated. Data were analyzed by means of regression. Plants reached the highest dry matter accumulation between 75 and 156 days after transplanting (DAT), which corresponded to the phase of greatest growth. Plants should be harvested between 60 and 87 DAT to achieve the highest stem yield. For the highest root yield, the harvest should be carried out 180 DAT. The period from 80 to 118 DAT was the most adequate for reaching simultaneously the highest yields for both roots and stems. Therefore, the ideal harvest time for sweet potatoes depends on the use intended for the plant and on the physiological indexes associated with it.O objetivo deste trabalho foi caracterizar o crescimento da planta e determinar a melhor época de colheita da batata-doce, considerando raízes e parte aérea (ramas). O trabalho foi realizado no campus JK da Universidade Federal dos Vales do Jequitinhonha e Mucuri, em Diamantina-MG. O delineamento experimental foi blocos ao acaso, com quatro repetições. Foram realizadas doze coletas consecutivas de três plantas centrais da parcela, em intervalos de quinze dias. As plantas foram fracionadas em raiz, caule e folhas para a determinação das suas respectivas massas secas. A área foliar também foi mensurada e foram calculados os índices de crescimento. Os dados obtidos foram analisados por meio de regressão. Entre 75 e 156 dias após o transplantio (DAT), ocorreu o maior acúmulo de massa seca pela planta, caracterizando a fase de maior crescimento. Para maior rendimento de ramas, as plantas devem ser colhidas entre 60 e 87 DAT; para maior rendimento de raízes, a colheita deve ser realizada 180 DAT. O período de 80 a 118 DAT foi o mais adequado para a colheita simultânea de raízes e ramas, obtendo-se as maiores produtividades para ambos. Logo, a época ideal de colheita na batata-doce depende da sua finalidade de uso e de índices fisiológicos associados aos objetivos do cultivo

Extended duration of the detectable stage by adding HPV test in cervical cancer screening

The human papillomavirus test (HPV) test could improve the (cost-) effectiveness of cervical screening by selecting women with a very low risk for cervical cancer during a long period. An analysis of a longitudinal study suggests that women with a negative Pap smear and a negative HPV test have a strongly reduced risk of developing cervical abnormalities in the years following the test, and that HPV testing lengthens the detectable stage by 2-5 years, compared to Pap smear detection alone

Distribution and abundance of key vectors of Rift Valley fever and other arboviruses in two ecologically distinct counties in Kenya

Background Rift Valley fever (RVF) is a mosquito-borne viral zoonosis of ruminants and humans that causes outbreaks in Africa and the Arabian Peninsula with significant public health and economic consequences. Humans become infected through mosquito bites and contact with infected livestock. The virus is maintained between outbreaks through vertically infected eggs of the primary vectors of Aedes species which emerge following rains with extensive flooding. Infected female mosquitoes initiate transmission among nearby animals, which amplifies virus, thereby infecting more mosquitoes and moving the virus beyond the initial point of emergence. With each successive outbreak, RVF has been found to expand its geographic distribution to new areas, possibly driven by available vectors. The aim of the present study was to determine if RVF virus (RVFV) transmission risk in two different ecological zones in Kenya could be assessed by looking at the species composition, abundance and distribution of key primary and secondary vector species and the level of virus activity. Methodology Mosquitoes were trapped during short and long rainy seasons in 2014 and 2015 using CO2 baited CDC light traps in two counties which differ in RVF epidemic risk levels(high risk Tana-River and low risk Isiolo),cryo-preserved in liquid nitrogen, transported to the laboratory, and identified to species. Mosquito pools were analyzed for virus infection using cell culture screening and molecular analysis. Findings Over 69,000 mosquitoes were sampled and identified as 40 different species belonging to 6 genera (Aedes, Anopheles, Mansonia, Culex, Aedeomyia, Coquillettidia). The presence and abundance of Aedes mcintoshi and Aedes ochraceus, the primary mosquito vectors associated with RVFV transmission in outbreaks, varied significantly between Tana-River and Isiolo. Ae. mcintoshi was abundant in Tana-River and Isiolo but notably, Aedes ochraceus found in relatively high numbers in Tana-River (n = 1,290), was totally absent in all Isiolo sites. Fourteen virus isolates including Sindbis, Bunyamwera, and West Nile fever viruses were isolated mostly from Ae. mcintoshi sampled in Tana-River. RVFV was not detected in any of the mosquitoes. Conclusion This study presents the geographic distribution and abundance of arbovirus vectors in two Kenyan counties, which may assist with risk assessment for mosquito borne diseases