80 research outputs found

    Discrimination, Crypticity, and Incipient Taxa in Entamoeba

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    Persistent difficulties in resolving clear lineages in diverging populations of prokaryotes or unicellular eukaryotes (protistan polyphyletic groups) are challenging the classical species concept. Although multiple integrated approaches would render holistic taxonomies, most phylogenetic studies are still based on single-gene or morphological traits. Such methodologies conceal natural lineages, which are considered “cryptic.” The concept of species is considered artificial and inadequate to define natural populations. Social organisms display differential behaviors toward kin than to nonrelated individuals. In “social” microbes, kin discrimination has been used to help resolve crypticity. Aggregative behavior could be explored in a nonsocial protist to define phylogenetic varieties that are considered “cryptic.” Two Entamoeba invadens strains, IP-1 and VK-1:NS are considered close populations of the same “species.” This study demonstrates that IP-1 and VK-1:NS trophozoites aggregate only with alike members and discriminate members of different strains based on behavioral and chemical signals. Combined morphological, behavioral/chemical, and ecological studies could improve Archamoebae phylogenies and define cryptic varieties. Evolutionary processes in which selection acted continuously and cumulatively on ancestors of Entamoeba populations gave rise to chemical and behavioral signals that allowed individuals to discriminate nonpopulation members and, gradually, to the emergence of new lineages; alternative views that claim a “Designer” or “Creator” as responsible for protistan diversity are unfounded

    HII regions in the CALIFA survey: I. Catalog presentation

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    We present a new catalogue of H II regions based on the integral field spectroscopy (IFS) data of the extended CALIFA and PISCO samples. The selection of H II regions was based on two assumptions: a clumpy structure with high contrast of H α emission and an underlying stellar population comprising young stars. The catalogue provides the spectroscopic information of 26 408 individual regions corresponding to 924 galaxies, including the flux intensities and equivalent widths of 51 emission lines covering the wavelength range between 3745 and 7200 Å. To our knowledge, this is the largest catalogue of spectroscopic properties of H II regions. We explore a new approach to decontaminate the emission lines from diffuse ionized gas contribution. This diffuse gas correction was estimated to correct every emission line within the considered spectral range. With the catalogue of H II regions corrected, new demarcation lines are proposed for the classical diagnostic diagrams. Finally, we study the properties of the underlying stellar populations of the H II regions. It was found that there is a direct relationship between the ionization conditions on the nebulae and the properties of stellar populations besides the physicals condition on the ionized regions.Fil: Espinosa Ponce, Carlos. Universidad Nacional Autónoma de México; MéxicoFil: Sánchez, S. F.. Universidad Nacional Autónoma de México; MéxicoFil: Morisset, C.. Universidad Nacional Autónoma de México; MéxicoFil: Barrera Ballesteros, J. K.. Universidad Nacional Autónoma de México; MéxicoFil: Galbany, Lluís. Universidad de Granada; EspañaFil: García Benito, Rubén. Instituto de Astrofísica de Andalucía; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Lacerda, E. A. D.. Universidad Nacional Autónoma de México; MéxicoFil: Mast, Damian. Archivo del Observatorio Astronomico de Cordoba ; Observatorio Astronomico de Cordoba ; Rectorado ; Universidad Nacional de Cordoba; . Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentin

    pyFIT3D and pyPipe3D -- The new version of the Integral Field Spectroscopy data analysis pipeline

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    We present a new version of the FIT3D and Pipe3D codes, two packages to derive properties of the stellar populations and the ionized emission lines from optical spectroscopy and integral field spectroscopy data respectively. The new codes have been fully transcribed to Python from the original Perl and C versions, modifying the algorithms when needed to make use of the unique capabilities of this language with the main goals of (1) respecting as much as possible the original philosophy of the algorithms, (2) maintaining a full compatibility with the original version in terms of the format of the required input and produced output files, and (3) improving the efficiency and accuracy of the algorithms, and solving known (and newly discovered) bugs. The complete package is freely distributed, with an available repository online. pyFIT3D and pyPipe3D are fully tested with data of the most recent IFS data surveys and compilations (e.g. CALIFA, MaNGA, SAMI and AMUSING++), and confronted with simulations. We describe here the code, its new implementation, its accuracy in recovering the parameters based on simulations, and a showcase of its implementation on a particular dataset.Comment: New Astronomy - 29 pages, 19 figures - Received on 7 Dec 2021 - Accepted for publication on 8 Jul 202

    Phylogenenetic approach of the section Bulbocodii D.C. of Narcissus based on cpDNA. A case of taxonomic inflation ?

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    In this paper, we analyzed the phylogeny of the section Bulbocodii (genus Narcissus; Amarillydaceae) using the matK and trnL-F fragments of cpDNA in order to review the validity of the recognized taxa. Our results indicate that Narcissus obesus should be considered a valid species, and that N. blancoi is a distinct taxon. In addition, seven previously recognized species, N. juressianus, N. subnivalis, N. graellsii, N. conspicuus, N. citrinus, N. nivalis, and N. quintanilhae, should be assigned to an infraspecific rank under N. bulbocodium, as they are not valid species. In addition, we analyzed the distribution of the three morphological characters widely used in the systematics of this section and found that their variation does not agree with the phylogenetic results, rendering these characters limited taxonomical utility. This result suggests that the section Bulbocodii shows high morphological lability, which can explain the proliferation of nominal speciesinfo:eu-repo/semantics/publishedVersio

    High Diversity of vacA and cagA Helicobacter pylori Genotypes in Patients with and without Gastric Cancer

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    BACKGROUND: Helicobacter pylori is associated with chronic gastritis, peptic ulcers, and gastric cancer. The aim of this study was to assess the topographical distribution of H. pylori in the stomach as well as the vacA and cagA genotypes in patients with and without gastric cancer. METHODOLOGY/PRINCIPAL FINDINGS: Three gastric biopsies, from predetermined regions, were evaluated in 16 patients with gastric cancer and 14 patients with dyspeptic symptoms. From cancer patients, additional biopsy specimens were obtained from tumor centers and margins; among these samples, the presence of H. pylori vacA and cagA genotypes was evaluated. Positive H. pylori was 38% and 26% in biopsies obtained from the gastric cancer and non-cancer groups, respectively (p = 0.008), and 36% in tumor sites. In cancer patients, we found a preferential distribution of H. pylori in the fundus and corpus, whereas, in the non-cancer group, the distribution was uniform (p = 0.003). A majority of the biopsies were simultaneously cagA gene-positive and -negative. The fundus and corpus demonstrated a higher positivity rate for the cagA gene in the non-cancer group (p = 0.036). A mixture of cagA gene sizes was also significantly more frequent in this group (p = 0.003). Ninety-two percent of all the subjects showed more than one vacA gene genotype; s1b and m1 vacA genotypes were predominantly found in the gastric cancer group. The highest vacA-genotype signal-sequence diversity was found in the corpus and 5 cm from tumor margins. CONCLUSION/SIGNIFICANCE: High H. pylori colonization diversity, along with the cagA gene, was found predominantly in the fundus and corpus of patients with gastric cancer. The genotype diversity observed across systematic whole-organ and tumor sampling was remarkable. We find that there is insufficient evidence to support the association of one isolate with a specific disease, due to the multistrain nature of H. pylori infection shown in this work
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