Autoantibody Profiling of Cerebrospinal Fluid From Affective Disorders and Schizophrenia Patients by Phage Display: Method Optimization and Evaluation of Selected Autoantigens As Candidate Biomarkers

Immunoproteomics approach applied on affective disorder and schizophrenia patient CSF samples yielded a number of candidate autoantigens. An overall analysis of those candidates revealed that impairment of myelination might have a role in etiology of such psychiatric disorders. Validations were based on phage ELISA that is applied on the most prominent autoantigen candidate obtained from phage display selection. In parallel, a conventional ELISA by recombinant peptide rather then purified phage, helped reproduce the results that imply a tendency of autoimmune reactivity in patient CSF samples compared to controls. Comparison of these results with the closest family members of the candidate protein mapping to the same N-terminal sequence revealed that, the sequence obtained by phage display is more antigenic than its homologues. Genomic expression profile of selected candidate and three other functionally related schizophrenia susceptibility genes were studied on mRNA in brain and spinal cord of mice, from postnatal day one until postnatal day forty-two. We could observe patterns of expression that may suggest a possible interplay of candidate proteins in the relevant neuronal maintenance mechanism. Our approach using cDNA phage display used in the identification of autoantigens in inflammatory and autoimmune diseases of the CNS is quite feasible despite several drawbacks like high false-positive number. Finally, our results may raise interest on myelination related proteins that may have roles in schizoaffective symptoms. However, strictly more effort has to be implemented to confirm and get more insight on our findings, for example developing mouse models and studying a broader range of serum and CSF samples

Determination of natural radioactivity levels in soil and travertine of the region of Tokat and Sivas, Turkey

WOS: 000429070100015In this study, the environmental radioactivity measurements for Tokat and Sivas provinces in the northeast of Turkey were performed. Using gamma ray spectrometry, the activity concentrations of natural radionuclides in soil and travertine samples (Th-232, Ra-226, and K-40) were determined. The annual effective dose equivalent, the absorbed doses rate in air, the radium equivalent, and the external hazard index were obtained from these activities. The activity concentrations vary from 9.09 to 17.04 Bq kg(-1) for Th-232, from 36.53 to 76.95 Bq kg(-1) for Ra-226, and from 216.56 to 576.59 Bq kg(-1) for K-40 in soil samples. The activity concentrations in travertines vary from 15.99 to 21.01 Bq kg(-1) for Th-232, from 19.89 to 67.71 Bq kg(-1) for Ra-226, and from 179.89 to 314.43 Bq kg(-1) for K-40. The average dose rate in air for soil and travertine samples was 43.41 and 41.05 nGy h(-1) respectively. The obtained results are presented and compared with other studies, and the results of this study are lower than the international recommended value (55 nGy h(-1)) given by UNSCEAR (2000). The results show that the region has a background radiation level within the natural limits.Gaziosmanpasa University Scientific Research Projects Department (BAP)Gaziosmanpasa University [24/2013]This work is supported by Gaziosmanpasa University Scientific Research Projects Department (BAP) under project no. 24/2013

Solution of the Bosonic and Algebraic Hamiltonians by using AIM

We apply the notion of asymptotic iteration method (AIM) to determine eigenvalues of the bosonic Hamiltonians that include a wide class of quantum optical models. We consider solutions of the Hamiltonians, which are even polynomials of the fourth order with the respect to Boson operators. We also demonstrate applicability of the method for obtaining eigenvalues of the simple Lie algebraic structures. Eigenvalues of the multi-boson Hamiltonians have been obtained by transforming in the form of the single boson Hamiltonian in the framework of AIM

The effects of streptozotocin-induced diabetes on ghrelin expression in rat testis: biochemical and immunohistochemical study

Introduction. Ghrelin is a hormone which has effects on the secretion of growth hormone, gastrointestinal system, cardiovascular system, cell proliferation and reproductive system. The present study we focused on the relation between ghrelin and GHS-R1a gene expression and the regulation of their expression in the testes of diabetic rats. Material and methods. 40 male Wistar albino rats were divided into four groups: control, and sampled 4, 8 and 12 weeks after induction of diabetes by streptozotocin (STZ) intraperitoneal injection (40 mg/kg). The rats were decapitated under ketamine anesthesia and their testes were removed. Blood was obtained from heart and serum follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone levels were measured by ELISA. Tissue ghrelin and GHS-R mRNA levels were determined by qRT-PCR, while ghrelin protein expression was studied by immunohistochemistry. Histopathological damage scores were also assessed. Results. Eight weeks after diabetes induction serum FSH level was increased, whereas LH and testosterone concentrations decreased. The ghrelin and GHS-R1a gene expression and ghrelin immunohistochemistry score first tended to increase after first four weeks of diabetes, and then tended to decrease. Ghrelin-immunopositive cells were detected in Leydig cells in all groups of rats, however, not in the germinal epithelium. Congestion of vessels and hemorrhage, formation of the vacuoles in spermatogonia and spermatocytes, desquamation of spermatids in the lumen and disorganization of seminiferous tubule germinal epithelium were observed in testis of all the diabetic rats. In addition, mean testicular biopsy score and mean seminiferous tubule diameter were getting lower in diabetic animals. Conclusion. Our results suggest that diabetes affects ghrelin expression in rat testis.

Characterization of deposits formed on diesel injectors in field test and from thermal oxidative degradation of n-hexadecane in a laboratory reactor

Solid deposits from commercially available high-pressure diesel injectors (HPDI) were analyzed to study the solid deposition from diesel fuel during engine operation. The structural and chemical properties of injector deposits were compared to those formed from the thermal oxidative stressing of a diesel fuel range model compound, n-hexadecane at 160°C and 450 psi for 2.5 h in a flow reactor. Both deposits consist of polyaromatic compounds (PAH) with oxygen moieties. The similarities in structure and composition of the injector deposits and n-hexadecane deposits suggest that laboratory experiments can simulate thermal oxidative degradation of diesel in commercial injectors. The formation of PAH from n-hexadecane showed that aromatization of straight chain alkanes and polycondensation of aromatic rings was possible at temperatures as low as 160°C in the presence of oxygen. A mechanism for an oxygen-assisted aromatization of cylcoalkanes is proposed

Generating Time-Varying Road Network Data Using Sparse Trajectories

While research on time-varying graphs has attracted recent attention, the research community has limited or no access to real datasets to develop effective algorithms and systems. Using noisy and sparse GPS traces from vehicles, we develop a time-varying road network data set where edge weights differ over time. We present our methodology and share this dataset, along with a graph manipulation tool. We estimate the traffic conditions using the sparse GPS data available by characterizing the sparsity issues and assessing the properties of travel sequence data frequency domain. We develop interpolation methods to complete the sparse data into a complete graph dataset with realistic time-varying edge values. We evaluate the performance of time-varying and static shortest path solutions over the generated dynamic road network. The shortest paths using the dynamic graph produce very different results than the static version. We provide an independent Java API and a graph database to analyze and manipulate the generated time-varying graph data easily, not requiring any knowledge about the inners of the graph database system. We expect our solution to support researchers to pursue problems of time-varying graphs in terms of theoretical, algorithmic, and systems aspects. The data and Java API are available at: http://elif.eser.bilkent.edu.tr/roadnetwork. © 2016 IEEE

POEMMA: Probe Of Extreme Multi-Messenger Astrophysics

The Probe Of Extreme Multi-Messenger Astrophysics (POEMMA) mission is being designed to establish charged-particle astronomy with ultra-high energy cosmic rays (UHECRs) and to observe cosmogenic tau neutrinos (CTNs). The study of UHECRs and CTNs from space will yield orders-of-magnitude increase in statistics of observed UHECRs at the highest energies, and the observation of the cosmogenic flux of neutrinos for a range of UHECR models. These observations should solve the long-standing puzzle of the origin of the highest energy particles ever observed, providing a new window onto the most energetic environments and events in the Universe, while studying particle interactions well beyond accelerator energies. The discovery of CTNs will help solve the puzzle of the origin of UHECRs and begin a new field of Astroparticle Physics with the study of neutrino properties at ultra-high energies.Comment: 8 pages, in the Proceedings of the 35th International Cosmic Ray Conference, ICRC217, Busan, Kore

Neuroactive steroids in depression and anxiety disorders: Clinical studies

Certain neuroactive steroids modulate ligand-gated ion channels via non-genomic mechanisms. Especially 3 alpha-reduced pregnane steroids are potent positive allosteric modulators of the gamma-aminobutyric acid type A (GABA(A)) receptor. During major depression, there is a disequilibrium of 3 alpha-reduced neuroactive steroids, which is corrected by clinically effective pharmacological treatment. To investigate whether these alterations are a general principle of successful antidepressant treatment, we studied the impact of nonpharmacological treatment options on neuroactive steroid concentrations during major depression. Neither partial sleep deprivation, transcranial magnetic stimulation, nor electroconvulsive therapy affected neuroactive steroid levels irrespectively of the response to these treatments. These studies suggest that the changes in neuroactive steroid concentrations observed after antidepressant pharmacotherapy more likely reflect distinct pharmacological properties of antidepressants rather than the clinical response. In patients with panic disorder, changes in neuroactive steroid composition have been observed opposite to those seen in depression. However, during experimentally induced panic induction either with cholecystokinine-tetrapeptide or sodium lactate, there was a pronounced decline in the concentrations of 3 alpha-reduced neuroactive steroids in patients with panic disorder, which might result in a decreased GABAergic tone. In contrast, no changes in neuroactive steroid concentrations could be observed in healthy controls with the exception of 3 alpha,5 alpha-tetrahydrodeoxycorticosterone. The modulation of GABA(A) receptors by neuroactive steroids might contribute to the pathophysiology of depression and anxiety disorders and might offer new targets for the development of novel anxiolytic compounds. Copyright (c) 2006 S. Karger AG, Basel

Estimation of the exposure for the air shower detection mode of EUSO-SPB1

EUSO-SPB1 was a balloon-borne pathfinder mission of the JEM-EUSO (Joint Experiment Missions for the Extreme Universe Space Observatory) program. A 12-day long flight started from New Zealand on April 25th, 2017 on-board the NASA's Super Pressure Balloon. With capability of detecting EeV energy air showers, the data acquisition was performed using a 1 m^2 two-Fresnel-lens UV-sensitive telescope with fast readout electronics in the air shower detection mode over ~30 hours at ~16--30 km above South Pacific. Using a variety of approaches, we searched for air shower events. Up to now, no air shower events have been identified. The effective exposure, regarding the role of the clouds in particular, was estimated based on the air shower and detector simulations together with a numerical weather forecast model. Compared with the case assuming the fully clear atmosphere conditions, more than ~60% of showers are detectable regardless the presence of the clouds. The studies in the present work will be applied in the follow-up pathfinders and in the future full-scale missions in the JEM-EUSO program