Mechanisms of Action and Targets of Nitric Oxide in the Oculomotor System

Nitric oxide (NO) production by neurons in the prepositus hypoglossi (PH) nucleus is necessary for the normal performance of eye movements in alert animals. In this study, the mechanism(s) of action of NO in the oculomotor system has been investigated. Spontaneous and vestibularly induced eye movements were recorded in alert cats before and after microinjections in the PH nucleus of drugs affecting the NO–cGMP pathway. The cellular sources and targets of NO were also studied by immunohistochemical detection of neuronal NO synthase (NOS) and NO-sensitive guanylyl cyclase, respectively. Injections of NOS inhibitors produced alterations of eye velocity, but not of eye position, for both spontaneous and vestibularly induced eye movements, suggesting that NO produced by PH neurons is involved in the processing of velocity signals but not in the eye position generation. The effect of neuronal NO is probably exerted on a rich cGMP-producing neuropil dorsal to the nitrergic somas in the PH nucleus. On the other hand, local injections of NO donors or 8-Br-cGMP produced alterations of eye velocity during both spontaneous eye movements and vestibulo-ocular reflex (VOR), as well as changes in eye position generation exclusively during spontaneous eye movements. The target of this additional effect of exogenous NO is probably a well defined group of NO-sensitive cGMP-producing neurons located between the PH and the medial vestibular nuclei. These cells could be involved in the generation of eye position signals during spontaneous eye movements but not during the VOR.Fondo de Investigación Sanitaria Grants 94/0388 and 97/2054Comunidad Autónoma de Madrid Grant 08.5/0019/1997Dirección General de Investigación Científica y Technológica Grant PB 93–117

Updating of Attentional and Premotor Allocation Resources as function of previous trial outcome

The neural bases of the inter-trial validity/invalidity sequential effects in a visuo-auditory modified version of the Central Cue Posner's Paradigm (CCPP) are analyzed by means of Early Directing Attention Negativity (EDAN), Contingent Negative Variation (CNV) and Lateralized Readiness Potential (LRP). ERPs results indicated an increase in CNV and LRP in trials preceded by valid trials compared to trials preceded by invalid trials. The CNV and LRP pattern would be highly related to the behavioral pattern of lower RTs and higher number of anticipations in trials preceded by valid with respect to trials preceded by invalid trials. This effect was not preceded by a modulation of the EDAN as a result of the previous trial condition. The results suggest that there is a trial-by-trial dynamic modulation of the attentional system as a function of the validity assigned to the cue, in which conditional probabilities between cue and target are continuously update

Cortical Dynamics during the Preparation of Antisaccadic and Prosaccadic Eye Movements in Humans in a Gap Paradigm

To compare the cortical dynamics of different oculomotor tasks, EEG and eye movements were recorded in 21 volunteers. Using a comprehensive approach, subjects were asked to perform saccadic tasks, which included a saccadic eye movement to a peripheral target (prosaccadic), a movement to the opposite side (antisaccadic), or maintain the gaze fixed (no-go). In mixed trials, prosaccadic, antisaccadic and no-go tasks were indicated by a color square (S1) present for 1900–2500 ms (instructive period). S1 disappeared for 370 ms (gap) and a black dot at 8 deg at right or left indicated the beginning of the task. Reaction times, amplitude of eye movements and number of errors were greatest in antisaccadic tasks, suggesting a greater difficulty. The EEG showed a contingent negativity variation (CNV) that increased progressively along the instructive period and suddenly during the gap: higher in antisaccadic, followed by prosaccadic and no-go tasks. Principal component analysis (PCA) disentangled fronto-central and occipital CNV-related and fronto-central gap-related components. The instructive period was characterized by fronto-central and occipital beta desynchronization (ERD) higher in antisaccadic than in no-go and parieto-occipital alpha synchronization higher in no-go than in antisaccadic tasks. During the gap, parieto-occipital beta and alpha ERD were higher in antisaccadic compared to no-go. The gap was further characterized by a fronto-central increase of inter-trial coherence in theta: highest during antisaccadic, followed by prosaccadic and no-go tasks. This phase locking in theta was also accompanied by theta ERS, which was significantly higher in antisaccadic than in the other two tasks. In PCA of spectral power two main components had dynamics similar to those extracted from voltage data, suggesting cross-frequency coupling. These results suggest that the more difficult saccadic tasks are associated with top-down control mediated by frontal cortex, while simpler tasks rely more on bottom-up control mediated by posterior cortices.España, Junta de Andalucía, Consejeria de Innovacion, Ciencia y Empresa P09-CVI-4712España, Junta de Andalucía, Consejeria de Innovacion, Ciencia y Empresa P07-CVI-02717España,Ministerio de Ciencia e innovación MCYT-BFU2008-0453

The Subversion of Male Literary Canon in the Female Gothic: The Yellow Wallpaper and “The Bloody Chamber”

Charlotte Perkins Gilman y Angela Carter, aunque separadas por un siglo de diferencia, se posicionan como activistas progresistas de un movimiento feminista que se opone al modelo más tradicional existentes en sus distintos periodos históricos. En línea con esta ideología más liberal que gira alrededor de una autodefinición de la mujer se crean sus respectivos relatos: The Yellow Wallpaper; (1892) y The Bloody Chamber (1979).Los elementos que definen a la literatura Gótica se subvierten en el Gótico femenino por ambas autoras, con la intención de crear discursos impregnados por una denuncia social, ya sea de forma argumentativa, temática o estilística. Como primer movimiento literario que logra una representación respetable de la autonomía y sexualidad femenina, este promueve el destronamiento de las ideas patriarcales impuestas sobre la mujer y permite que ellas escriban sus propias historiasPor consiguiente, el propósito de este Trabajo Final de Grado es revisar los elementos pertenecientes al Gótico femenino de las dos historias ya mencionadas, así como su relevancia en los contextos del feminismo de los siglos XIX y XX, además de realizar un análisis de cómo las autoras presentan las consecuencias dañinas de la propia implicación de la mujer en su opresión.<br /

A Cholinergic Synaptically Triggered Event Participates in the Generation of Persistent Activity Necessary for Eye Fixation

An exciting topic regarding integrative properties of the nervous system is how transient motor commands or brief sensory stimuli are able to evoke persistent neuronal changes, mainly as a sustained, tonic action potential firing. A persisting firing seems to be necessary for postural maintenance after a previous movement. We have studied in vitro and in vivo the generation of the persistent neuronal activity responsible for eye fixation after spontaneous eye movements. Rat sagittal brainstem slices were used for the intracellular recording of prepositus hypoglossi (PH) neurons and their synaptic activation from nearby paramedian pontine reticular formation (PPRF) neurons. Single electrical pulses applied to the PPRF showed a monosynaptic glutamatergic projection on PH neurons, acting on AMPA-kainate receptors. Train stimulation of the PPRF area evoked a sustained depolarization of PH neurons exceeding (by hundreds of milliseconds) stimulus duration. Both duration and amplitude of this sustained depolarization were linearly related to train frequency. The train-evoked sustained depolarization was the result of interaction between glutamatergic excitatory burst neurons and cholinergic mesopontine reticular fibers projecting onto PH neurons, because it was prevented by slice superfusion with cholinergic antagonists and mimicked by cholinergic agonists. As expected, microinjections of cholinergic antagonists in the PH nucleus of alert behaving cats evoked a gaze-holding deficit consisting of a re-centering drift of the eye after each saccade. These findings suggest that a slow, cholinergic, synaptically triggered event participates in the generation of persistent activity characteristic of PH neurons carrying eye position signals.Unión Europea Grants BI04-CT98-0546España, Ministerio de Ciencia PB98-0011, BFI2000-00936, BFI2000-1190, y BFI2002-0137

Constructive heuristics for the unrelated parallel machines scheduling problem with machine eligibility and setup times

This work considers a scheduling problem identified in a factory producing customised Heating, Ventilation and Air Conditioning (HVAC) equipment. More specifically, the metal folding section is modelled as unrelated parallel machines with machine eligibility and sequence-dependent setup times. The objective under consideration is the minimisation of the total tardiness. The problem is known to be NP-hard so approximate methods are needed to solve real-size instances. In order to embed the scheduling procedure into a decision support system providing high-quality solutions in nearly real time, the goal of this paper is to develop fast, efficient constructive heuristics for the problem. Due to the lack of methods for this specific problem, some existing heuristics and one metaheuristic are selected from related problems and adapted. In addition, a set of heuristics with novel repair and improvement phases are proposed. The performance of the methods adapted and the proposals are compared with the optimal/approximate solutions obtained by a solver for an MILP in two sets of instances with small and medium sizes. Additionally, big-size instances (representing more realistic cases for our company) have been solved using the proposed constructive heuristics, providing efficient solutions in negligible computational times. Even if the adaptation of heuristics performs reasonably well, these are outperformed by the new heuristic proposed in this paper. In addition, when the new heuristic is embedded in the metaheuristic adapted from a related the problem, the results obtained are excellent in terms of the quality of the solution, even if the computational effort is somewhat higher.Ministerio de Ciencia en Innovación. “PROMISE

Alternatives for Locating People Using Cameras and Embedded AI Accelerators: A Practical Approach

Presented at the 4th XoveTIC Conference, A Coruña, Spain, 7–8 October 2021.[Abstract] Indoor positioning systems usually rely on RF-based devices that should be carried by the targets, which is non-viable in certain use cases. Recent advances in AI have increased the reliability of person detection in images, thus, enabling the use of surveillance cameras to perform person localization and tracking. This paper evaluates the performance of indoor person location using cameras and edge devices with AI accelerators. We describe the video processing performed in each edge device, including the selected AI models and the post-processing of their outputs to obtain the positions of the detected persons and allow their tracking. The person location is based on pose estimation models as they provide better results than do object detection networks in occlusion situations. Experimental results are obtained with public datasets to show the feasibility of the solution.This work has been funded by the Navantia-UDC Joint Research Unit under Grant IN853B-2018/02, the Xunta de Galicia (by grant ED431C 2020/15, and grant ED431G 2019/01 to support the Centro de Investigación de Galicia “CITIC”), the Agencia Estatal de Investigación of Spain (by grants RED2018-102668-T and PID2019-104958RB-C42) and ERDF funds of the EU (FEDER Galicia 2014–2020 & AEI/FEDER Programs, UE).Xunta de Galicia; IN853B-2018/02Xunta de Galicia; ED431C 2020/15Xunta de Galicia; ED431G 2019/0

Assembling Nonplanar Polyaromatic Units by Click Chemistry. Study of Multicorannulene Systems as Host for Fullerenes

Producción CientíficaNovel compounds with two or three corannulene subunits have been obtained by “click chemistry”. These exotic systems were synthesized in high yields using the ethynylcorannulene as common reagent. The synergistic action as receptors for fullerenes of several corannulene blocks has been evaluated. It was found that the three-armed derivatives showed efficient complexation abilities toward C60. Furthermore, a new compound having two corannulene subunits linked to a hexahelicene scaffold has a remarkable affinity constant. Finally, theoretical calculations have been performed to evaluate the formation of their relative adducts containing a C60 molecule.Ministerio de Economía, Industria y Competitividad (Projects 258 CTQ2013-41067-P and CTQ2011-23862-C02

Impact of medium-scale traveling ionospheric disturbances on network real-time kinematic services: CATNET study case

Medium-scale traveling ionospheric disturbances (MSTIDs) are fluctuations in the plasma density that propagate through the upper layer of the atmosphere at velocities of approximately 100 m/s and periods reaching some tens of minutes. Due to their wavelengths, MSTIDs can degrade the performance of differential positioning techniques, such as real-time kinematics (RTK) or network-RTK (NRTK). This paper defines a novel methodology as a tool for relating the errors in NRTK positioning based on an MSTIDs indicator using the second difference in time of the slant total electron content (STEC). The pro-posed methodology performs integer ambiguity resolution (IAR) on the undifferenced measurements instead of using double-differenced carrier-phase measurements, as it is usual in RTK and NRTK. Statistical tests are applied to evaluate the degradation in the position errors caused by the impacts of MSTIDs on RTK and NRTK positioning over a data set spanning one year gathered from the CATNET network; a dual-frequency network of fixed permanent GNSS receivers located at the mid-latitudes of northeastern Spain. With the development of the proposed methodology for measuring the position degradation, another results of the present research are the establishment of thresholds for the proposed MSTIDs index, which can be used to monitor the positioning solution and to warn users when the measurements are affected by MSTIDs events, relating the position error to MSTIDs that affect not only the user receivers but also of the reference receivers within the network.Peer ReviewedPostprint (published version

A dual-wavelength fiber laser sensor with temperature and strain discrimination

This work presents a dual-wavelength C-band erbium-doped fiber laser assisted by an artificial backscatter reflector. This fiber-based reflector, inscribed by femtosecond laser direct writing, was fabricated into a single mode fiber with a length of 32 mm. The dual-wavelength laser obtained, centered at 1527.7 nm and 1530.81 nm, showed an optical signal-to-noise ratio over 46 dB when pumped at 150 mW. Another feature of this laser was that the power difference between the two channels was just 0.02 dB, regardless of the pump power, resulting in a dual emission laser with high equalization. On the other hand, an output power level and a central wavelength instability as low as 0.3 dB and 0.01 nm were measured, in this order for both channels. Moreover, the threshold pump power was 40 mW. Finally, the performance of this dual-wavelength fiber laser enhanced with a random reflector for sensing applications was studied, achieving the simultaneous measurement of strain and temperature with sensitivities around 1 pm/µε and 9.29 pm/°C, respectively.This work is part of the projects PID2019-107270RB, funded by MCIN/AEI/10.13039/ 501100011033 and FEDER 'A way to make Europe', and PDC2021-121172 funded by MCIN/AEI/10.13039/ 501100011033 and European Union 'Next generation EU'/PTR, the Ministerio de Educación, Cultura y Deporte of Spain (PhD grant FPU2018/02797)