69 research outputs found

    Urorectal septum malformation sequence in a calf

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    Rainwater Harvesting in Large Residential Buildings in Australia

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    Australia is one of the driest inhabited continents, with highly variable rainfall. A growing urban population and frequent droughts in recent years have made water supply a major issue in Australia. A number of alternative water sources have received attention in Australia including rainwater harvesting, grey water reuse and wastewater recycling. Among these, rainwater harvesting has received the greatest attention as rainwater is fresh in nature and can be easily collected and used for non-potable purposes. However, many Australians still show a reluctance to adopt rainwater harvesting systems. Statistics from the Australian Bureau of Statistics (ABS) show that about 47% of respondents say that the main reason for not installing a rainwater tank is the perceived ‘higher cost’ (ABS, 2011). Government authorities in Australia provide financial incentives to encourage home owners to install rainwater tanks. For example, Sydney Water Corporation in Australia offers a rainwater tank rebate of up to 1,500(here1,500 (here is in Aus$) depending on the size of the tank installed and the type of water use

    Sarkoid donjeg očnog kapka kod magarca (Equus asinus) izazvan bovinim papiloma 2 virusom

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    Sarcoid tumors were described by means of histopathological and molecular procedures in a 5-year-old donkey. Histopathological examination showed epithelial changes including hyperkeratosis, epithelial hyperplasia, koilocytosis, and rete peg formation. Neoplastic fibroblastic cells were plumb, large spindle to stellate and embedded in dense collagenous tissue. Results of Polymerase Chain Reaction and DNA sequence analysis showed that the etiological agent belonged to Bovine Papilloma Virus-II species in the delta papilloma virus genus. This case study represents the first report demonstrating the presence of Bovine Papilloma Virus-II in donkey sarcoid.Sarkoidi su opisani primenom histopatoloških i molelularanih metoda i procedura kod magarca, starog pet godina. Histopatološko ispitivanje, ukazalo je na promene u epitelu koje su uključivale hiperkeratozu, hiperplaziju epitela, koilocitozu i formiranje invaginacija epitela ptema dubokom dermisu - rete peg. Fibroblastine neoplastične ćelije su bile krupne, ovalnovretenaste i zvezdaste, uronjene u tkivo bogato kolagenom. Ispitivanje PCR metodom kao i rezultati DNK sekvencioniranja pokazali su da je etiološki agens spadao u grupu bovinog papilomavirusa-2 koji pripada rodu delta papilomavirusa. Ova studija predstavlja prvi opisani slučaj prisustva papiloma virusa goveda-2 kod sarkoida magarca

    The histopathological evaluation of effects of application of the bovine amniotic fluid with graft on peri-implant bone regeneration

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    This study aimed to determine the effects of bovine amniotic fluid combined with bone graft in treating peri-implant bone defects with guided bone regeneration. Twenty female Sprague–Dawley rats were divided into two groups. Bone sockets with a diameter of 4 mm in the coronal part and a diameter of 2.5 mm in the apical part of the implant were created into the corticocancellous bone in the metaphyseal parts of the right tibia bones of all subjects. Implants with a length of 4 mm and a diameter of 2.5 mm were placed in the bone sockets. In the sham surgery group (n = 10) was the circumferential bone defect equivalent to half of the 4-mm implant length, which occurred between the implant and the bone, filled with bovine xenograft. Bovine xenografts were filled with amniotic fluid mixture in the experimental group (n = 10). After 8 weeks of recovery, all rats were sacrificed. The implants were extracted from the soft tissues and the surrounding bone. Subsequently, the bones were decalcified and prepared for histological analysis. The percentage of newly regenerated bone (NRB) formation and fibrosis in the bone defect area around the implant was calculated from all sections. NRB was found in 37.4±4.4% of controls and 41.4±2.63% of test animals (P<0.05 and P=0.024, respectively). Fibrosis formation was found at a rate of 38.6±5.06% in the control group and 33.2±5.38% in the test group (P<0.05 and P=0.033, respectively). It was considered that combining bovine amniotic fluid with bone transplant could be a useful way of treating bone abnormalities

    Comparison of the effects of polyethylene glycol and hyaluronic acid in prevention of epidural fibrosis

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    The aim of this study was to evaluate of the effect of 5% Polyethylene Glycol 4000 in the prevention of epidural fibrosis and to compare the results with hyaluronic acid. A total of 36 adult New Zealand White Rabbits were used in the study. Three groups were formed with each group including 12 rabbits (Group I: Control, Group II: 5% Polyethylene Glycol 4000, Group III: Hyaluronic acid). Hemilaminectomy was performed at L5 vertebrae on the right side. Three rabbits randomly selected from each group at postoperative 2nd, 4th, 8th and 12th week were euthanized. Macroscopic and histopathological evaluation were performed in terms of the amount of scar tissues. Statistical evaluation was made with Mann-Whitney U Test and Kruskal-Wallis Variance Analysis. P < 0.05 was considered statistically significant. In the hemilaminectomy sites of Group I, severe thickness and tenacious epidural scar adhesions were found between the dura mater and surrounding tissues. In the Group II, dura mater was clean without any evidence of adhesion or scar tissue and minimal adhesions were present. In the Group III, moderate adhesion and scar tissue or minimal adhesion and very thin scar tissue were observed. Statistically; Group II was significantly different from Group I (P < 0.000) and Group III (P < 0.002), and Group III was significantly different from Group I (P < 0.001). It was concluded that 5% Polyethylene glycol 4000 can safely be used locally during operation in the area of decompression in order to prevent epidural fibrosis encountered as a postoperative complication after hemilaminectomy

    Traumatic reticuloperitonitis in a flock of sheep

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    (The objective of this study was to describe the clinical, ultrasonographical, and pathological findings of traumatic reticuloperitonitis (TRP) with its complications in a flock of sheep. Thirteen sheep in a flock died within one month's duration before two sheep were admitted to the Firat University Veterinary Medical Teaching Hospital. Three additional dead sheep were submitted for necropsy. A tentative diagnosis of TRP on two of the sheep was made based on clinical and ultrasonographic findings. A needle perforating the reticulum was removed from each sheep by surgery. The two sheep improved after the surgery. The necropsy revealed fibrous adhesions and a fistular connection between the dorso-ventral face of the reticulum to diaphragma and pericardium with 3 cm syringe needles in the other three sheep. There were congophylic amyloid deposits in the glomerular tufts, Bowman capsule and vascular walls of the liver, kidneys and spleen. Additionally, there was centrilobular necrosis indicating heart failure.Taken together; TRP is rarely seen in sheep; however, TRP and its complications may cause economic losses in sheep flocks. Ultrasonographic examination of the reticular area in sheep may be used as an ancillary diagnostic technique

    Increased plasma cardiac troponin I concentration in lambs with myocarditis

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    Background Cardiac troponin I (cTnI) is a blood biomarker of myocardial injury. A human cTnI assay may be useful for measuring cTnI concentrations in lambs with naturally occurring myocarditis. Objective The aims of this study were to evaluate the utility of a commercially available human chemiluminescent microparticle cTnI immunoassay for measuring plasma cTnI concentrations in lambs with naturally occurring myocarditis from infection with foot and mouth disease virus (FMDV), and to determine cTnI expression in cardiac muscle of affected lambs. Methods Ten lambs with myocarditis and 10 clinically healthy lambs (control group) were included. Clinical signs, gross and histologic necropsy findings, and immunoreactivity for cTnI in cardiac tissue were evaluated. Plasma cTnI concentration was determined using the commercial human immunoassay system. Results All lambs with myocarditis died within 1 similar to day of clinical signs. Infection with FMDV was confirmed by PCR analysis. Gross cardiac lesions were evident and histologic examination revealed myocarditis. Immunoreactivity for cTnI was absent in cardiac myocytes that were degenerative or necrotic, but was strong in cardiac myocytes from unaffected areas of the myocardium and in all cardiac myocytes of healthy lambs. The geometric mean plasma concentrations of cTnI for lambs in the myocarditis and control groups were 146.78 similar to mu g/L (95% confidence interval [CI], 61.90348.06) and 0.013 similar to mu g/L (95% CI, 0.0100.017), respectively (t-value 19.27; P similar toBackgroundCardiac troponin I (cTnI) is a blood biomarker of myocardial injury. A human cTnI assay may be useful for measuring cTnI concentrations in lambs with naturally occurring myocarditis.ObjectiveThe aims of this study were to evaluate the utility of a commercially available human chemiluminescent microparticle cTnI immunoassay for measuring plasma cTnI concentrations in lambs with naturally occurring myocarditis from infection with foot and mouth disease virus (FMDV), and to determine cTnI expression in cardiac muscle of affected lambs.MethodsTen lambs with myocarditis and 10 clinically healthy lambs (control group) were included. Clinical signs, gross and histologic necropsy findings, and immunoreactivity for cTnI in cardiac tissue were evaluated. Plasma cTnI concentration was determined using the commercial human immunoassay system.ResultsAll lambs with myocarditis died within 1&nbsp;day of clinical signs. Infection with FMDV was confirmed by PCR analysis. Gross cardiac lesions were evident and histologic examination revealed myocarditis. Immunoreactivity for cTnI was absent in cardiac myocytes that were degenerative or necrotic, but was strong in cardiac myocytes from unaffected areas of the myocardium and in all cardiac myocytes of healthy lambs. The geometric mean plasma concentrations of cTnI for lambs in the myocarditis and control groups were 146.78&nbsp;&mu;g/L (95% confidence interval [CI], 61.90&ndash;348.06) and 0.013&nbsp;&mu;g/L (95% CI, 0.010&ndash;0.017), respectively (t-value 19.27; P&nbsp;&lt;&nbsp;.0001).ConclusionsA commercial human cTnI assay may be used to detect plasma cTnI concentrations in sheep, and cTnI may be used as a blood-based biomarker of myocarditis in this species.</p

    Koinfekcija teladi virusom papularnog stomatitisa goveda, rotavirusom i Cryptosporidium Spp

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    Concurrent occurence of bovine papular stomatitis, rotavirus infection and cryptosporidiosis was diagnosed postmortem in a 7-days-old calf from a farm containing 65 calves of different ages. Multifocal papular stomatitis and rumenitis were present on necropsy. While polymerase chain reaction analysis revealed rotavirus and papular stomatitis virus infections; bovine viral diarrhea, foot and mouth disease, bovine papilloma virus and coronavirus could not be detected. Overall; concurrent co-infection with bovine papular stomatitis virus, rotavirus and cryptosporidium spp. was reported for the first time.Postmortalno je dijagnostikovana infekcija virusom papuloznog stomatitisa, rotavirusna infekcija kao i kriptosporidioza kod teleta starog 7 dana, na farmi sa 65 teladi različitog uzrasta. Prilikom obdukcije, uočene su multifokalne lezije karakteristične za papulozni stomatitis kao i rumenitis. Testom lančane reakcije polimeraze (PCR) dokazano je prisustvo rotavirusa i virusa izazivača papuloznog stomatitisa, pri čemu nisu dokazani virusi izazivači bovine virusne dijareje, slinavke i šapa, papilomatoze i koronavirusne infekcije. Uopšteno, ovim je po prvi put je prikazan slučaj koinfekcije virusom izazivačem papuloznog stomatitisa, rotavirusne infekcije i infekcija sa Cryptosporidium spp

    Rainwater tanks in multi-unit buildings : a case study for three Australian cities

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    Rainwater tanks have become popular in large Australian cities due to water shortage and greater public awareness towards sustainable urban development. Rainwater harvesting in multi-unit buildings in Australia is less common. This paper investigates the water savings potential of rainwater tanks fitted in multi-unit residential buildings in three cities of Australia: Sydney, Newcastle and Wollongong. It is found that for multi-unit buildings, a larger tank size is more appropriate to maximise water savings. It is also found that rainwater tank of appropriate size in a multi-unit building can provide significant mains water savings even in dry years. A prediction equation is developed which can be used to estimate average annual water savings from having a rainwater tank in a multi-unit building in these three Australian cities
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