Role for miR-204 in human pulmonary arterial hypertension

Reduced miR-204 expression facilitates the excessive proliferation and apoptosis resistance of pulmonary artery smooth muscle cells characteristic of human pulmonary arterial hypertension

Integrated Analyses of Copy Number Variations and Gene Expression in Lung Adenocarcinoma

Numerous efforts have been made to elucidate the etiology and improve the treatment of lung cancer, but the overall five-year survival rate is still only 15%. Identification of prognostic biomarkers for lung cancer using gene expression microarrays poses a major challenge in that very few overlapping genes have been reported among different studies. To address this issue, we have performed concurrent genome-wide analyses of copy number variation and gene expression to identify genes reproducibly associated with tumorigenesis and survival in non-smoking female lung adenocarcinoma. The genomic landscape of frequent copy number variable regions (CNVRs) in at least 30% of samples was revealed, and their aberration patterns were highly similar to several studies reported previously. Further statistical analysis for genes located in the CNVRs identified 475 genes differentially expressed between tumor and normal tissues (p<10−5). We demonstrated the reproducibility of these genes in another lung cancer study (p = 0.0034, Fisher's exact test), and showed the concordance between copy number variations and gene expression changes by elevated Pearson correlation coefficients. Pathway analysis revealed two major dysregulated functions in lung tumorigenesis: survival regulation via AKT signaling and cytoskeleton reorganization. Further validation of these enriched pathways using three independent cohorts demonstrated effective prediction of survival. In conclusion, by integrating gene expression profiles and copy number variations, we identified genes/pathways that may serve as prognostic biomarkers for lung tumorigenesis

Evaluating the potential for double cropping in Canada: effect of seeding date and relative maturity on the development and yield of maize, white bean and soybean

Double cropping is not presently a common practice in Canada. The long-term climate averages, however, suggest that the practice should be possible in the most southern portions of the country. The study described herein represents the first simultaneous evaluation of three crops, maize, soybean and white bean, seeded at five seeding dates spanning late June through early August in the most southern region of Canada. Germplasm was chosen such that physiological maturity could theoretically be reached if seeded following winter wheat. Results indicate that, following summer seeding, development in all three crops was primarily driven by thermal requirements. Only the pod filling stage of soybean was influenced by the declining daylengths of autumn and this effect was most pronounced in germplasm of longer relative maturity. Yields of white bean and maturity group 00 soybean were unaffected by seeding up to the third week of July whereas yield of maize and higher maturity group soybeans declined from June onward. For the latter, declining yields were primarily attributable to the interaction of seeding date and relative maturity and their effect on season length. These results clearly demonstrate that with the appropriate selection of germplasm, there is a seeding date window where maize, soybean or white bean can be expected to reach physiological maturity as a double crop in Canada.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Refuge facilitates the preservation and accumulation of herbicide resistance traits in Conyza canadensis

Herbicide resistance is selected for within a weed population through the recurrent use of an herbicide. Once the use of the herbicide is reduced or discontinued, the trait may persist in the population unless resistance endows a fitness penalty. Few studies have examined the long-term persistence of an herbicide resistance trait in a weed population once the selection pressure that led to its prevalence has been removed. The objective of this research was to re-survey the locations described by A.J.J. Smisek in her 1995 study of a paraquat resistant biotype of C. canadensis on the grounds of the Harrow Research and Development Centre. Results indicate that, ∼20 yr after paraquat use was significantly reduced, a glyphosate resistant biotype had replaced the paraquat resistant biotype at all but one of the original locations surveyed by Smisek. The sole location where the paraquat resistant biotype was observed was an old, un-treed orchard row currently managed by mowing. This location not only served as a refuge for the paraquat resistant biotype, ensuring its persistence in the face of strong selection for glyphosate resistance at all other locations, but it facilitated the co-occurrence of glyphosate and paraquat resistant biotypes. The observation of a multiple resistant biotype at this location alone, with resistance to both paraquat and glyphosate, suggests a role for refugia in the stacking of herbicide resistance traits. Given that this stacking occurred in the absence of either herbicide, we hypothesize that the multiple resistant biotype arose through pollen mediated gene flow among biotypes.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Acute Pseudohepatitis in a Chronic Substance Abuser Secondary to Occult Seat Belt Injury

Causes of a massive elevation in serum aminotransferases (aspartate aminotransferase [AST] and alanine aminotransferase [ALT]) in the substance-abusing patient include viral hepatitis and drug hepatotoxicity. A patient chronically addicted to injection heroin and cocaine presented to the emergency room in a confused state and was admitted to a medical ward with an AST of 4120 U/L, ALT 3820 U/L and right upper quadrant discomfort. Investigations for viral and hepatotoxic causes for the liver dysfunction revealed only hepatitis C seropositivity. A computed tomogram of the abdomen, however, revealed a significant contusion to the right lobe of the liver consistent with traumatic injury. A motor vehicle accident, in which the patient was wearing a seat belt, and which had occurred a few days before admission and had been thought to be minor, was the cause of the liver dysfunction. Significant blunt abdominal traumatic injuries are usually managed exclusively by surgical trauma units. This case underlines the need for medical specialists to be aware of hepatic contusion injuries and to have a high index of suspicion when investigating unexplained hepatocellular dysfunction in chronic substance abusers who have been in motor vehicle accidents

Effect of seeding date on winter canola (Bassica napus L.) yield and oil quality in southern Ontario

Winter canola or winter oilseed rape (Brassica napus L.) is not commonly grown in Canada. While winter oilseed rape is the dominant growth form in Europe, Canadian canola production is dominated by spring types in western Canada. Research conducted in the 1980s indicated that the environmental conditions in southern Ontario are well suited to the production of winter canola. Since then, however, interest in the crop has ebbed and little to no research has been conducted on the agronomic issues that potentially limit its adoption in the province. The objective of this research was to identify an optimal seeding date for winter canola in southern Ontario. Three winter canola hybrids were evaluated across five seeding dates ranging from early September to late October. Results established the first two weeks of September as the optimal seeding period for winter canola in southern Ontario. Seeding winter canola during this period, such that greater than 600 GDD could be accumulated before the first fall frost, not only reduced winterkill to approximately 20% but also maximized yield potential and ensured optimal oil quality. Winter canola showed great potential for production in southern Ontario and its addition to current crop rotations would diversify and enhance crop production practices in this portion of the province.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Tricotelydenous giant ragweed (Ambrosia trifida L.).

In this short communication we report the observation of a tricotelydenous giant ragweed (Ambrosia trifida L.) individual. In contrast to the normal dicotelydenous pattern of phyllotaxy, this individual was observed to express three axillary meristems at each node that intersected the main stem at angles of 120 degrees.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Black Carbon Emissions in Gasoline Exhaust and a Reduction Alternative with a Gasoline Particulate Filter

Black carbon (BC) mass and solid particle number emissions were obtained from two pairs of gasoline direct injection (GDI) vehicles and port fuel injection (PFI) vehicles over the U.S. Federal Test Procedure 75 (FTP-75) and US06 Supplemental Federal Test Procedure (US06) drive cycles on gasoline and 10% by volume blended ethanol (E10). BC solid particles were emitted mostly during cold-start from all GDI and PFI vehicles. The reduction in ambient temperature had significant impacts on BC mass and solid particle number emissions, but larger impacts were observed on the PFI vehicles than the GDI vehicles. Over the FTP-75 phase 1 (cold-start) drive cycle, the BC mass emissions from the two GDI vehicles at 0 °F (−18 °C) varied from 57 to 143 mg/mi, which was higher than the emissions at 72 °F (22 °C; 12–29 mg/mi) by a factor of 5. For the two PFI vehicles, the BC mass emissions over the FTP-75 phase 1 drive cycle at 0 °F varied from 111 to 162 mg/mi, higher by a factor of 44–72 when compared to the BC emissions of 2–4 mg/mi at 72 °F. The use of a gasoline particulate filter (GPF) reduced BC emissions from the selected GDI vehicle by 73–88% at various ambient temperatures over the FTP-75 phase 1 drive cycle. The ambient temperature had less of an impact on particle emissions for a warmed-up engine. Over the US06 drive cycle, the GPF reduced BC mass emissions from the GDI vehicle by 59–80% at various temperatures. E10 had limited impact on BC emissions from the selected GDI and PFI vehicles during hot-starts. E10 was found to reduce BC emissions from the GDI vehicle by 15% at standard temperature and by 75% at 19 °F (−7 °C)

N-Terminal Ubiquitination of Extracellular Signal-Regulated Kinase 3 and p21 Directs Their Degradation by the Proteasome

Extracellular signal-regulated kinase 3 (ERK3) is an unstable mitogen-activated protein kinase homologue that is constitutively degraded by the ubiquitin-proteasome pathway in proliferating cells. Here we show that a lysineless mutant of ERK3 is still ubiquitinated in vivo and requires a functional ubiquitin conjugation pathway for its degradation. Addition of N-terminal sequence tags of increasing size stabilizes ERK3 by preventing its ubiquitination. Importantly, we identified a fusion peptide between the N-terminal methionine of ERK3 and the C-terminal glycine of ubiquitin in vivo by tandem mass spectrometry analysis. These findings demonstrate that ERK3 is conjugated to ubiquitin via its free NH(2) terminus. We found that large N-terminal tags also stabilize the expression of the cell cycle inhibitor p21 but not that of substrates ubiquitinated on internal lysine residues. Consistent with this observation, lysineless p21 is ubiquitinated and degraded in a ubiquitin-dependent manner in intact cells. Our results suggests that N-terminal ubiquitination is a more prevalent modification than originally recognized