A Pilot Study of the Impact of Metaphylactic Treatment at Processing on Lung Lesions at Slaughter

The utility of metaphylactic antibiotic/anti-inflammatory treatment in prevention of cattle lung lesions was examined. Sixty-nine, single source, ranch direct, fall weaned steers were allocated to treatment or no treatment groups at feedlot arrival in February, 1999. Treatment consisted of single subcutaneous administration of NuflorTM (florfenicol, Schering-Plough Animal Health) at 18 mg/lb bodyweight and BanamineTM (flunixin meglumine, Schering-Plough Animal Health) at 1 mg/lb bodyweight. All steers were weighed, vaccinated with a modified live IBR/PI3 vaccine, implanted, and identified by ear tag. Cattle were fed for maximum gain on a corn-based diet. At harvest, after 133 days on feed, lungs were observed for lesions indicative of previous pneumonia and scored using an established system. Hot carcass weight, quality grade, and yield grade was collected on each carcass. Results indicate that while lung lesions were prevalent (43.3% of cattle affected), treatment had no effect on the prevalence of lung lesions at slaughter. In addition, lung lesions were not associated with feedlot average daily gain or quality grade. \u27This small study suggests that prevalence of lung lesions in low risk cattle will not be affected by administration of metaphylactic treatment with Nuflor/Banamine at processing

Pasture Weaning and Forage Barley to Extend the Grazing Season for Replacement Heifers

In a two-year study at the SDSU Cow/Calf Teaching and Research Unit, Brookings, SD, heifer calves were allotted to two weaning management treatments in early October at an average age of 202 days. The pasture-weaned group was separated from their dams and grazed grass pasture across the fence from their dams for two weeks. Then, until early December, they grazed “Robust” barley (forage type) that had been no-till planted into oat stubble in early August. The drylot-weaned group was fed a traditional weaning diet of grass hay, corn, and protein supplement from weaning until early December. The effect of management on heifer weight gain depended on year. In the first year, pasture-weaned heifers gained more than the drylot group during the first two weeks after weaning. Due to less than ideal pasture conditions in the second year, the drylot group gained more than the pasture weaned group for two and four weeks after weaning. In both years, gains from weaning to the end of the grazing period in December and to the following April were similar between management systems. Pasture weaning appeared to cause less stress for both cows and calves, but no differences in disease symptoms were observed. Antibody titers for IBR, BVD type 1 and BVD type 2 were determined at weaning and two and four weeks after weaning to measure the development of immunity from vaccinations administered two months prior to and at weaning. At two weeks after weaning in the second year of the study, antibody titer for BVD type 1 was greater for the drylot group than the pasture-weaned group. By four weeks after weaning the pasture-weaned heifers had antibody titers similar to the drylot group. The results of this study indicate that pasture weaning combined with small grain pasture to extend the grazing system can be an effective alternative for managing replacement heifers compared to a traditional drylot weaning system

Effect of Calving Time and Weaning Time on Feedlot Performance - A Preliminary Report

Cows grazing native range year round at the SDSU Cottonwood Research Station were allotted to 3 management systems; Group 1) a calving season starting in mid March with calves weaned in late October, Group 2) a calving season starting in mid March with calves weaned in mid September, and Group 3) a calving season starting early May with calves weaned in late October. Following weaning the steer calves are transported to the Southeast South Dakota Experiment Farm. For two calf crops the steer calves have been fed a high grain diet for maximum gain from weaning to harvest. Group 2 had a lower mean average daily gain than Groups 1 and 3. Feed conversion was not affected by treatment. There was not a consistent indication that groups weaned at a younger age (Groups 2 and 3) had more health problems than Group 1. Group 3 had the highest mean dressing percentage and carcass weight. Weaning at a younger age and a longer time on feed resulted in higher marbling scores for Group 2 compared to Group 1. This advantage in marbling was not observed for Group 3

Defining Failure of Passive Transfer in South Dakota Beef Calves

Failure of calves to ingest and absorb immunoglobulin from colostrum is a risk factor for illness and decreased performance. Blood samples were taken from 752 calves at three SDSU research units. Total protein in blood, closely correlated to colostral immunoglobulin absorption, was determined and calf health records were collected. Using this data, a classification table of sensitivity and specificity was constructed to determine the relationship between total protein and calf illness and to classify calves as having adequate colostral absorption or inadequate colostral absorption (failure of passive transfer). Along with sensitivity and specificity, positive and negative likelihood ratios were calculated to identify a suitable cutoff point to separate calves that would become ill from those that would remain healthy. The cutoff point selected was a serum total protein level of 5.5 g/dL, which produced a sensitivity of 30% and specificity of 87%. Calves with total protein levels below 5.5 g1dL were 3.07 (95% CI 1.73-5.43, p=0.0002) times as likely to become ill as calves with total protein levels above 5.5 g/dL. In beef production situations similar to those in these herds, producers should be able to limit disease if calves\u27 total protein at 24 hours following birth is equal to or greater than 5.5 g/dL

Assessment of Diversity of Antimicrobial Resistance Phenotypes and Genotypes of \u3ci\u3eMannheimia haemolytica\u3c/i\u3e Isolates from Bovine Nasopharyngeal Swabs

The threat of bovine respiratory disease (BRD) for cattle operations is exacerbated by increasing prevalence of antimicrobial resistance (AMR) in Mannheimia haemolytica, a leading cause of BRD. Characterization of AMR in M. haemolytica by culture and susceptibility testing is complicated by uncertainty regarding the number of colonies that must be selected to accurately characterize AMR phenotypes (antibiograms) and genotypes in a culture. The study objective was to assess phenotypic and genotypic diversity of M. haemolytica isolates on nasopharyngeal swabs (NPS) from 28 cattle at risk for BRD or with BRD. NPS were swabbed onto five consecutive blood agar plates; after incubation up to 20 M. haemolytica colonies were selected per plate (up to 100 colonies per NPS). Phenotype was determined by measuring minimum inhibitory concentrations (MIC) for 11 antimicrobials and classifying isolates as resistant or not. Genotype was indirectly determined by matrix-assisted laser desorption/ionization time of flight mass spectroscopy (MALDI-TOF MS). NPS from 11 of 28 cattle yielded at least one M. haemolytica isolate; median (range) of isolates per NPS was 48 (1–94). NPS from seven cattle yielded one phenotype, 3 NPS yielded two, and 1 NPS yielded three; however, within a sample all phenotypic differences were due to only oneMIC dilution. On each NPS all M. haemolytica isolated were the same genotype; genotype 1 was isolated from three NPS and genotype two was isolated from eight. Diversity of M. haemolytica on bovine NPS was limited, suggesting that selection of few colonies might adequately identify relevant phenotypes and genotypes

Patterns and correlates of drug-related ED visits: Results from a national survey

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/78042/1/66.pd

Measurement of inclusive D*+- and associated dijet cross sections in photoproduction at HERA

Inclusive photoproduction of D*+- mesons has been measured for photon-proton centre-of-mass energies in the range 130 < W < 280 GeV and a photon virtuality Q^2 < 1 GeV^2. The data sample used corresponds to an integrated luminosity of 37 pb^-1. Total and differential cross sections as functions of the D* transverse momentum and pseudorapidity are presented in restricted kinematical regions and the data are compared with next-to-leading order (NLO) perturbative QCD calculations using the "massive charm" and "massless charm" schemes. The measured cross sections are generally above the NLO calculations, in particular in the forward (proton) direction. The large data sample also allows the study of dijet production associated with charm. A significant resolved as well as a direct photon component contribute to the cross section. Leading order QCD Monte Carlo calculations indicate that the resolved contribution arises from a significant charm component in the photon. A massive charm NLO parton level calculation yields lower cross sections compared to the measured results in a kinematic region where the resolved photon contribution is significant.Comment: 32 pages including 6 figure

Measurement of Jet Shapes in Photoproduction at HERA

The shape of jets produced in quasi-real photon-proton collisions at centre-of-mass energies in the range $134-277$ GeV has been measured using the hadronic energy flow. The measurement was done with the ZEUS detector at HERA. Jets are identified using a cone algorithm in the $\eta - \phi$ plane with a cone radius of one unit. Measured jet shapes both in inclusive jet and dijet production with transverse energies $E^{jet}_T>14$ GeV are presented. The jet shape broadens as the jet pseudorapidity ($\eta^{jet}$) increases and narrows as $E^{jet}_T$ increases. In dijet photoproduction, the jet shapes have been measured separately for samples dominated by resolved and by direct processes. Leading-logarithm parton-shower Monte Carlo calculations of resolved and direct processes describe well the measured jet shapes except for the inclusive production of jets with high $\eta^{jet}$ and low $E^{jet}_T$. The observed broadening of the jet shape as $\eta^{jet}$ increases is consistent with the predicted increase in the fraction of final state gluon jets.Comment: 29 pages including 9 figure

Dissociation of virtual photons in events with a leading proton at HERA

The ZEUS detector has been used to study dissociation of virtual photons in events with a leading proton, gamma^* p -> X p, in e^+p collisions at HERA. The data cover photon virtualities in two ranges, 0.03<Q^2<0.60 GeV^2 and 2<Q^2<100 GeV^2, with M_X>1.5 GeV, where M_X is the mass of the hadronic final state, X. Events were required to have a leading proton, detected in the ZEUS leading proton spectrometer, carrying at least 90% of the incoming proton energy. The cross section is presented as a function of t, the squared four-momentum transfer at the proton vertex, Phi, the azimuthal angle between the positron scattering plane and the proton scattering plane, and Q^2. The data are presented in terms of the diffractive structure function, F_2^D(3). A next-to-leading-order QCD fit to the higher-Q^2 data set and to previously published diffractive charm production data is presented

Cerebral hypometabolism in carriers of the intron 10 +3 MAPT mutation

poster abstractIntroduction: Multiple systems tauopathy with presenile dementia (MSTD), a form of frontotemporal dementia with parkinsonism-17 (FTDP-17), is a neurodegenerative disorder caused by an (a) to (g) transition at position +3 of intron 10 of the microtubule associated protein tau (MAPT) gene. The mutation causes over-expression of 4 repeat (4R) tau isoforms with increased 4R/3R ratio leading to neurodegeneration. Clinically, these patients primarily present with behavior variant FTD (bvFTD), showing disinhibition, and disordered social comportment, as well as impaired executive function, memory, and speech. While altered glucose metabolism has been reported in subjects with sporadic bvFTD, it has yet to be reported in an MSTD sample of this size carrying the intron 10 + 3 mutation. In this study, we used voxel-based analysis to assess brain metabolism using fluorodeoxyglucose (FDG) positron emission tomography (PET) in eleven mutation carriers and eight non-carriers. Methods: Eleven MAPT intron 10 + 3 mutation carriers (5 males; mean age = 48.0 +/- 6.9 years) and eight non-carriers (2 males; mean age = 43.7 +/- 12.0 years) were imaged using FDG PET with standard techniques. Briefly, dynamic PET imaging for 60 minutes followed an intravenous injection of 5-10 mCi of FDG. Scans were then reconstructed using standard techniques, pre-processed for motion correction, and normalized to MNI space. A static FDG image from 30-60 minutes was created from the appropriate frames and normalized to a cerebellar gray matter reference region to create an SUVR image for each participant. These SUVR images were then assessed on a voxel-wise basis for the effect of mutation carrier status, covaried for age at scan and gender and masked using a whole-brain mask. Results were displayed at a voxel-wise threshold of p<0.01 (uncorrected) and minimum cluster size (k) = 50 voxels. SPM8 was used for all pre-processing and voxel-wise statistical analyses. Results: Eight of the MAPT intron 10 + 3 mutation carriers showed mild cognitive impairment at the time of the PET scan (MMSE = 25.3 +/- 2.4), while three MAPT intron 10 + 3 carriers were not impaired at the time of scan (MMSE = 28.0 +/- 0.0). Non-carriers had no cognitive impairment at the time of PET scan (MMSE = 27.1 +/- 1.6). Overall, MAPT mutation carriers showed lower FDG uptake bilaterally in the hippocampus, parahippocampal gyrus, amygdala, superior parietal lobule, and in the prefrontal cortex compared to non-carriers. Conclusions: The present findings suggest individuals with the MAPT mutation at position +3 of intron 10 show symmetrical glucose hypometabolism relative to non-carriers in the medial temporal lobe, parietal cortex, and frontal cortex. These metabolic changes overlap previously described patterns of neurodegeneration in MSTD patients and are consistent with the characteristics of their cognitive dysfunction