HUBUNGAN ANTARA DUKUNGAN SOSIAL DENGAN KECEMASAN MENGHADAPI DUNIA KERJA PADA MAHASISWA TINGKAT AKHIR DI FAKULTAS FARMASI DAN ILMU KESEHATAN UNIVERSITAS SARI MUTIARA INDONESIA

This study aims to determine the relationship between social support and anxiety to face the world of  work at the final level students. The hypothesis proposed is the existence of a negative relationship between, social support with anxiety facing the world of work at the final level students. The subjecks in this study were as many as 100 final year students in the Faculty of  Pharmacy and Health Sciences. The sampling technique in this study was the proportionate stratified random sampling technique used because the population had members/ elements that were homogeneous and proportionately proportional. The measuring instrument used in this study is the scale of social support and scale of anxiety facing the world of work, based on the validity and reliability on the scale of social support obtained 49 valid items with Alpha Cronbach reliability coefficient if the Alpha value >0.60 reliable. Obtained 14 valid items with Alpha Cronbach realibility coefficient if the Alpha value >0.60 reliabel data analysis method used is a statistical method using Paerson Product Moment.Significance value is 0.369 if Sig 0.05 the data meets linear. Based on the result of hypothesis testing, it can be seen that the hypothesis tested in this study was accepted. Thus, it can be concluded that there is a significant, and negative, relationship between social support and anxiety the world of work at the final level students

Análise de elementos químicos em tecidos humanos com e sem patologia tumoral utilizando fluorescência de raios-X

O cancro é uma das principais causas de morte a nível mundial, pelo que é essencial o desenvolvimento de investigação que possa conduzir à descoberta de novos biomarcadores tumorais. A técnica de Fluorescência de Raios-X tem-se revelado adequada para a análise dos elementos químicos presentes em tecidos humanos, nomeadamente para comparação entre tecidos normais e tumorais. No entanto, para uma correta determinação quantitativa destes elementos é necessária a avaliação da matriz escura destas amostras, contendo os elementos não detetados pela técnica utilizada. Num estudo inicial, procedeu-se à determinação da matriz dos tecidos humanos para utilização do método dos Parâmetros Fundamentais como método de quantificação. Foram analisados 4 Materiais de Referência Certificados e testadas diferentes composições de matriz com o objetivo de alcançar o melhor resultado possível. A matriz obtida consiste em 10% H, 22% C, 3% N e 60% O e foi validada por comparação com outro método de exatidão já comprovada (método comparativo com padrões), tendo-se demonstrado a sua aplicabilidade na quantificação deste tipo de amostras. Analisaram-se 39 pares de amostras de tecido humano incluídas em parafina por Micro- Fluorescência de Raios-X, sendo cada par normal-tumor pertencente ao mesmo indivíduo. Os tipos de tumor estudados foram os seguintes: adenocarcinomas do cólon e carcinomas da mama do tipo não-especial. Para quantificação utilizou-se o método dos Parâmetros Fundamentais pela aplicação da matriz determinada anteriormente. As áreas a quantificar em cada amostra foram escolhidas com base numa rotina desenvolvida em ambiente R. A análise estatística dos resultados foi feita com recurso ao software OriginPro®, tendo sido aplicados o teste de normalidade Shapiro-Wilk e o teste não-paramétrico Kruskal-Wallis ANOVA. Observou-se que no cólon, os elementos P, Fe e Cu apresentam concentrações significativamente mais elevadas nos tecidos tumorais, enquanto na mama os elementos que têm concentrações significativamente superiores em tecido tumoral são P, S, Ca, Fe, Cu e Zn. Concluiu-se que o P e o Fe têm potencialidade como biomarcadores tumorais do cólon. Por outro lado, os elementos P, S, Ca, Fe e Zn apresentam-se como possíveis biomarcadores tumorais da mama. O Cu revelou ser um constituinte da parafina em que as amostras estão incluídas, pelo que não deve ser avaliado em amostras com este material.Cancer is one of the leading causes of death worldwide, so it is essential to develop research that can lead to the discovery of new tumour biomarkers. The X-Ray Fluorescence technique has proved to be adequate for the analysis of the chemical elements present in human tissues, namely for comparison between normal and tumour tissues. However, for a correct quantitative determination of these elements it is necessary to evaluate the dark matrix of these samples, which contains the elements that are not detected by the technique used for this purpose. In an initial study, the matrix of human tissues was determined for use with the Fundamental Parameters method as a quantification approach. Four Certified Reference Materials were analysed and different matrix compositions were tested in order to achieve the best possible result. The obtained matrix is composed of 10% H, 22% C, 3% N and 60% O and it has been validated by comparison with another method of proven accuracy (comparative method with standards). Its applicability in the quantification of this type of samples was demonstrated. 39 pairs of human tissue samples embedded in paraffin were analysed by Micro-X-Ray Fluorescence, each normal-tumour pair belonging to the same subject. The types of tumour studied were: adenocarcinomas of the colon and carcinomas of the breast of no special type. For quantification, the Fundamental Parameters method was used by the application of the matrix previously determined. The areas for quantification were chosen based on a routine developed in R environment. Statistical analysis of the results was performed using the OriginPro® software, the Shapiro-Wilk normality test and the Kruskal-Wallis ANOVA non-parametric test being applied. It was observed that in the colon, the elements P, Fe and Cu have significantly higher concentrations in tumour tissues, while in the breast the elements with significantly higher concentrations in tumour tissues are P, S, Ca, Fe, Cu and Zn. It was found that P and Fe have potential as tumour biomarkers for colon. On the other hand, the elements P, S, Ca, Fe and Zn are possible tumour biomarkers for breast. Cu has been shown to be a constituent of the paraffin in which the samples are embedded, so it should not be evaluated in samples embedded in this material

Struktur Komunitas Fitoplankton di Waduk Cengklik Boyolali

Sebagian besar pemanfaatan lahan disekitar Waduk Cengklik adalah sawah irigasi dan pemukiman sehingga terdapat kegiatan pertanian dan rumahtangga. Di dalam badan waduk terdapat berbagai kegiatan yaitu pariwisata, budidaya ikan air tawar di dalam Keramba Jaring Apung, dan kegiatan pertanian. Kegiatan tersebut dapat meningkatkan penghasilan dan kesejahteraan, namun dilain pihak menyumbang sejumlah bahan organik ke dalam badan perairan waduk. Bahan organik yang tinggi akan menyebabkan eutrofikasi atau pengkayaan nutrien pada perairan. Penelitian ini bertujuan untuk menganalisis dan mengetahui struktur komunitas fitoplankton berdasarkan densitas, dominansi, dan indeks keanekaragaman dan hubungan antara indeks keanekaragaman fitoplankton dengan faktor abiotik perairan. Pengambilan sampel dilakukan pada pukul 10.00 – 13.00 WIB di tiga stasiun pengamatan dengan masing-masing tiga kali ulangan pengambilan sampel pada 3 waktu pengambilan yaitu 09 Juni 2015, 23 Juni 2015, dan 07 Juli 2015. Sampel yang diperoleh dianalisis dan diidentifikasi di Laboratorium Pusat FMIPA UNS dan Laboratorium Penguji dan Kalibrasi BBTKL PP Yogyakarta. Hasil dari penelitian ini diperoleh struktur komunitas fitoplankton yang terdiri dari 53 jenis fitoplankton dari filum Cyanophyta, Chlorophyta, Pyrrophyta, Euglenophya, dan Chrysophyta. Densitas fitoplankton berkisar antara 18.889 – 167.333 sel/L dan indeks keanekaragaman rendah dengan kestabilan komunitas fitoplankton moderat yang berkisar antara 1,06 – 2,38 serta terdapat jenis yang mendominasi yaitu Cyclotella kutzingiana, Nitzschia actinastroides, Euglena gracilis, Closteriopsis longissima, Volvox sp., dan Spirogyra azygospora. Terdapat hubungan antara nilai indeks keanekaragaman fitoplankton dengan faktor abiotik fisik – kimia yaitu suhu, kecerahan, pH, oksigen terlarut, nitrat, dan fosfat di Waduk Cengklik Boyolali. Kata kunci: Fitoplankton, Struktur Komunitas, Waduk Cengkli

Epileptic seizure after treatment with thiocolchicoside

International audienceBackground: Adverse drug reactions are important determinants of inpatient and outpatient morbidity. Thiocolchicoside is a semisynthetic derivate of naturally occurring colchicoside, which is largely used in humans as a centrally acting muscle relaxant. Epileptic seizures after thiocolchicoside intake have been reported in individuals with a history of epilepsy, acute brain injury or possible blood-brain barrier disruption.Case report: We report the case of a 66-year-old male patient presenting a sudden epileptic seizure temporally related to the intake of thiocolchicoside for muscle contracture and pain. The probably causes of the seizures were thiocolchicoside intake and cerebral microhemorrhages attributed to cerebral amyloid angiopathy.Discussion: Drugs only rarely cause focal seizures. Our case indicates that thiocolchicoside can precipitate seizures in predisposed patients, and that its use should be avoided in patients with brain diseases (and therefore lower seizure thresholds) or blood-brain barrier disruption. This information should be provided in the drug package insert

A non-destructive X-ray fluorescence method of analysis of formalin fixed-paraffin embedded biopsied samples for biomarkers for breast and colon cancer

Authors acknowledge Centro Hospitalar Barreiro-Montijo for allowing the current investigation and providing the samples. Publisher Copyright: © 2023 The AuthorsIn this work we present a methodology for the non-destructive elemental determination of formalin-fixed paraffin-embedded (FFPE) human tissue samples based on the Fundamental Parameters method for the quantification of micro Energy Dispersive X Ray Fluorescence (micro-EDXRF) area scans. This methodology intended to overcome two major constraints in the analysis of paraffin embedded tissue samples – retrieval of optimal region of analysis of the tissue within the paraffin block and the determination of the dark matrix composition of the biopsied sample. This way, an image treatment algorithm, based on R® tool to select the regions of the micro-EDXRF area scans was developed. Also, different dark matrix compositions were evaluated using varying combinations of H, C, N and O until the most accurate matrix was found: 8% H, 15% C, 1% N and 60% O for breast FFPE samples and 8% H, 23% C, 2% N and 55% O for colon. The developed methodology was applied to paired normal-tumour samples of breast and colon biopsied tissues in order to gauge potential elemental biomarkers for carcinogenesis in these tissues. The obtained results showed distinctive biomarkers for breast and for colon: there was a significant increase of P, S, K and Fe in both tissues, while a significant increase of Ca an Zn concentrations was also determined for breast tumour samples.publishersversionpublishe

Drug hypersensitivity in students from São Paulo, Brazil

BACKGROUND: Drug hypersensitivity is responsible for substantial mortality and morbidity, and increased health costs. However, epidemiological data on drug hypersensitivity in general or specific populations are scarce. METHODS: We performed a cross-sectional survey of 1015 university students, using a self-reported questionnaire. RESULTS: The prevalence of self-reported drug hypersensitivity was 12,11% (123/1015). The most frequently implicated drugs were non-steroidal anti-inflammatory drugs (45,9%) and beta-lactam and sulfonamide antibiotics (25,40%). The majority of the patients reported dermatological manifestations (99), followed by respiratory (40), digestive (23) and other (19). Forty-five patients had an immediate type reaction, and 76,72% (89) had the drug by oral route. CONCLUSION: The results showed that drug hypersensitivity is highly prevalent in university students, and that nonsteroidal anti-inflammatory drug and antibiotics (beta-lactams and sulfonamide) are the most frequently concerned drugs

Association between desloratadine and prednisolone in the treatment of children with acute symptoms of allergic rhinitis: a double-blind, randomized and controlled clinical trial

Introduction: A combination of antihistamines and oral corticosteroids is often used to treat acute symptoms of allergic rhinitis. Objective: To evaluate safety and efficacy of desloratadine plus prednisolone in the treatment of acute symptoms of children (2-12 years) with allergic rhinitis, and to compare it to dexchlorpheniramine plus betamethasone. Methods: Children with moderate/severe persistent allergic rhinitis and symptomatic (nasal symptoms score [0-12] >= 6) were allocated in a double-blind, randomized fashion to receive dexchlorpheniramine plus betamethasone (n = 105; three daily doses) or desloratadine plus prednisolone (n = 105; single dose followed by two of placebo) for 7 days. At the beginning and end of the evaluation, the following were obtained: nasal symptoms score, extra nasal symptoms score, peak nasal inspiratory flow, blood biochemistry, and electrocardiogram. Ninety-six children of the dexchlorpheniramine plus betamethasone group and 98 of the desloratadine plus prednisolone group completed the protocol. Results: The two groups were similar regarding initial and final nasal symptoms scores, extra nasal symptoms scores and peak nasal inspiratory flow. A drop of 76.4% and 79.1% for nasal symptoms score, 86.0% and 79.2% for extra nasal symptoms score, as well as an increase of 25.2% and 24.3% for peak nasal inspiratory flow occurred for those treated with desloratadine plus prednisolone and dexchlorpheniramine plus betamethasone, respectively. There were no significant changes in blood chemistry. Sinus tachycardia was the most frequent electrocardiogram change, but with no clinical significance. Drowsiness was reported significantly more often among those of dexchlorpheniramine plus betamethasone group (17.14% x 8.57%, respectively). Conclusion: The desloratadine plus prednisolone combination was able to effectively control acute symptoms of rhinitis in children, improving symptoms and nasal function. Compared to the dexchlorpheniramine plus betamethasone combination, it showed similar clinical action, but with a lower incidence of adverse events and higher dosing convenience. (C) 2016 Published by Elsevier Editora Ltda. on behalf of Associacao Brasileira de Otorrinolaringologia e Cirurgia Cervico-Facial.Funding authority for Studies and Projects (FINEP) - Innovation and Research, BrazilEMS/AS - Sao Paulo, BrazilUniv Fed Sao Paulo Unifesp, EPM, Dept Pediat, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, EPM, Dept Ginecol, Sao Paulo, SP, BrazilFundacao Apoio Escola Paulista Med FAP, Sao Paulo, SP, BrazilUniv Santo Amaro Unisa, Clin Med, Sao Paulo, SP, BrazilHosp Nipo Brasileiro, Pediat, Sao Paulo, SP, BrazilUniv Estadual Campinas Unicamp, Campinas, SP, BrazilFundacao Getulio Vargas, Mkt, Rio De Janeiro, RJ, BrazilGrp NC Farma, Sao Paulo, SP, BrazilGrp NC Farma, Pesquisa Clin & Farmacovigilancia, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, EPM, Dept Pediat, Sao Paulo, SP, BrazilUniv Fed Sao Paulo Unifesp, EPM, Dept Ginecol, Sao Paulo, SP, BrazilFundacao Apoio Escola Paulista Med FAP, Sao Paulo, SP, BrazilEMS/AS - Sao Paulo, Brazil: 01.12.0094.00EMS/AS - Sao Paulo, Brazil: FINEP-1375/10Web of Scienc

How Infection and Vaccination Are Linked to Acute and Chronic Urticaria: A Special Focus on COVID-19

Since more than a century ago, there has been awareness of the connection between viral infections and the onset and exacerbation of urticaria. Our knowledge about the role of viral infection and vaccination in acute and chronic urticaria improved as a result of the COVID-19 pandemic but it has also highlighted knowledge gaps. Viral infections, especially respiratory tract infections like COVID-19, can trigger the onset of acute urticaria (AU) and the exacerbation of chronic urticaria (CU). Less frequently, vaccination against viruses including SARS-CoV-2 can also lead to new onset urticaria as well as worsening of CU in minority. Here, with a particular focus on COVID-19, we review what is known about the role of viral infections and vaccinations as triggers and causes of acute and chronic urticaria. We also discuss possible mechanistic pathways and outline the unmet needs in our knowledge. Although the underlying mechanisms are not clearly understood, it is believed that viral signals, medications, and stress can activate skin mast cells (MCs). Further studies are needed to fully understand the relevance of viral infections and vaccinations in acute and chronic urticaria and to better clarify causal pathways