Genomic Epidemiology and Evolution of Duck Hepatitis A Virus

Duck hepatitis A virus (DHAV), an avian picornavirus, causes high-mortality acute disease in ducklings. Among the three serotypes, DHAV-1 is globally distributed, whereas DHAV-2 and DHAV-3 serotypes are chiefly restricted to Southeast Asia. In this study, we analyzed the genomic evolution of DHAV-1 strains using extant GenBank records and genomic sequences of 10 DHAV-1 strains originating from a large disease outbreak in 2004–2005, in Hungary. Recombination analysis revealed intragenotype recombination within DHAV-1 as well as intergenotype recombination events involving DHAV-1 and DHAV-3 strains. The intergenotype recombination occurred in the VP0 region. Diversifying selection seems to act at sites of certain genomic regions. Calculations estimated slightly lower rates of evolution of DHAV-1 (mean rates for individual protein coding regions, 5.6286 × 10−4 to 1.1147 × 10−3 substitutions per site per year) compared to other picornaviruses. The observed evolutionary mechanisms indicate that whole-genome-based analysis of DHAV strains is needed to better understand the emergence of novel strains and their geographical dispersal

Carbonatite and highly peralkaline nephelinite melts from Oldoinyo Lengai Volcano, Tanzania: The role of natrite-normative fluid degassing

Publisher's version (útgefin grein)Oldoinyo Lengai, located in the Gregory Rift in Tanzania, is a world-famous volcano owing to its uniqueness in producing natrocarbonatite melts and because of its extremely high CO2 flux. The volcano is constructed of highly peralkaline [PI = molar (Na2O + K2O)/Al2O3 > 2–3] nephelinite and phonolites, both of which likely coexisted with carbonate melt and a CO2-rich fluid before eruption. Results of a detailed melt inclusion study of the Oldoinyo Lengai nephelinite provide insights into the important role of degassing of CO2-rich vapor in the formation of natrocarbonatite and highly peralkaline nephelinites. Nepheline phenocrysts trapped primary melt inclusions at 750–800 °C, representing an evolved state of the magmas beneath Oldoinyo Lengai. Raman spectroscopy, heating-quenching experiments, low current EDS and EPMA analyses of quenched melt inclusions suggest that at this temperature, a dominantly natritess-normative, F-rich (7–14 wt%) carbonate melt and an extremely peralkaline (PI = 3.2–7.9), iron-rich nephelinite melt coexisted following degassing of a CO2 + H2O-vapor. We furthermore hypothesize that the degassing led to re-equilibration between the melt and liquid phases that remained and involved 1/ mixing between the residual (after degassing) alkali carbonate liquid and an F-rich carbonate melt and 2/ enrichment of the coexisting nephelinite melt in alkalis. We suggest that in the geological past similar processes were responsible for generating highly peralkaline silicate melts in continental rift tectonic settings worldwide.This study was financially supported by project NRDIO ( National Research, Development, and Innovation Office of Hungary ) K-119535 (to M. Berkesi and T. Guzmics) and by the Betta Üzletlánc Ltd. to Guzmics. In addition, M. Berkesi acknowledges to the ELTE Institutional Excellence Program (1783-3/2018/FEKUTSRAT) supported by the Hungarian Ministry of Human Capacities . We thank Toshiaki Tsunogae for his editorial handling, and Alan Cooper and an anonymous reviewer for their constructive comments that helped to improve the manuscript.Peer Reviewe

Melt-rock interaction in the lower crust based on silicate melt inclusions in mafic garnet granulite xenoliths, Bakony–Balaton Highland Volcanic Field (Hungary)

Major and trace element composition of silicate melt inclusions (SMI) and their rock-forming minerals were studied in mafic garnet granulite xenoliths from the Bakony-Balaton Highland Volcanic Field (Western Hungary). Primary SMIs occur in clinopyroxene and plagioclase in the plagioclase-rich domains of mafic garnet granulites and in ilmenite in the vicinity of these domains in the wall rock. Based on major and trace elements, we demonstrated that the SMIs have no connection with the xenolith-hosting alkaline basalt as they have rhyodacitic composition with a distinct REE pattern, negative Sr anomaly, and HFSE depletion. The trace element characteristics suggest that the clinopyroxene hosted SMIs are the closest representation of the original melt percolated in the lower crust. In contrast, the plagioclase and ilmenite hosted SMIs arc products of interaction between the silicic melt and the wall rock garnet granulite. A further product of this interaction is the clinopyroxene-ilmeniteiplagioclase symplectite. Textural observations and mass balance calculations reveal that the reaction between titanite and the silicate melt led to the formation of these assemblages. We propose that a tectonic melange of metapelites and (MOR-related) metabasalts partially melted at 0.3-0.5 GPa to form a dacitic-rhyodacitic melt leaving behind a garnet-free, plagioclaseclinopyroxene+orthopyroxene+ilmenite residuum. The composition of the SMIs (both major and trace elements) is similar to those from the middle Miocene calc-alkaline magmas, widely known from the northern Pannonian Basin (BOrzsOny and Visegrad Mts., Cserhat and Matra volcanic areas and Central Slovakian VF), but the SMIs are probably the result of a later, local process. The study of these SMIs also highlights how crustal contamination changes magma compositions during asthenospheric Miocene ascent.ISSN:1335-0552ISSN:1336-805

Novel Circoviruses from Birds Share Common Evolutionary Roots with Fish Origin Circoviruses

Circoviruses occur in a variety of animal species and are common pathogens of mammalian and avian hosts. In our study internal organ samples of wild birds were processed for screening of circoviral sequences. Two novel viruses were identified and characterized in specimens of a little bittern and a European bee-eater that suffered from wing injuries, were weakened, had liver or kidney failures, and finally succumbed at a rescue station. The 1935 nt and 1960 nt long viral DNA genomes exhibited a genomic structure typical for circoviruses and were predicted to encode replication-associated protein in the viral strand, and a capsid protein in the complementary strand of the replicative intermediate DNA form. The genome of the newly described viruses showed 37.6% pairwise identity with each other and ≤41.5% identity with circovirus sequences, and shared a common branch with fish, human and Weddel seal circoviruses in the phylogenetic tree, implying evolutionary relationship among the ancestors of these viruses. Based on the results the little bittern and European bee-eater circoviruses represent two distinct species of the Circovirus genus, Circoviridae family

Geothermal energy and ore-forming potential of 600 °C mid-ocean-ridge hydrothermal fluids

The ~4500-m-deep Iceland Deep Drilling Project (IDDP) borehole IDDP-2 in Iceland penetrated the root of an active seawater-recharged hydrothermal system below the Mid-Atlantic Ridge. As direct sampling of pristine free fluid was impossible, we used fluid inclusions to constrain the in situ conditions and fluid composition at the bottom of the hydrothermal convection cell. The fluid temperature is ~600 °C, and its pressure is near-hydrostatic (~45 MPa). The fluid exists as two separate phases: an H2O-rich vapor (with an enthalpy of ~59.4 kJ/mol) and an Fe-K–rich brine containing 2000 μg/g Cu, 3.5 μg/g Ag, 1.4 μg/g U, and 0.14 μg/g Au. Occasionally, the fluid inclusions coexist with rhyolite melt inclusions. These findings indicate that the borehole intersected high-energy steam, which is valuable for energy production, and discovered a potentially ore-forming brine. We suggest that similar fluids circulate deep beneath mid-ocean ridges worldwide and form volcanogenic massive sulfide Cu-Zn-Au-Ag ore deposits