Live Cell Imaging of Bone Marrow Stromal Cells on Nano-pitted and Polished Titanium Surfaces: A Micro-Incubator in vitro Approach

Current orthopedic implants are not conducive for optimal integration of the biomaterial with newly-formed tissue (osseointegration) inside a patient’s body. In this study, medical-rade Ti-6Al-4V was used as a substrate due to its biocompatibility and ability to facilitate cellular adhesion and proliferation. Live cell imaging was conducted on bone marrow stromal cells, genetically modified to express the green fluorescent protein (GFP), from the 24-96 hours growth period, with the first 24 hours of growth being held inside a lab-scale incubator. Periodic images were recorded on nanopitted anodized and polished Ti-6Al-4V substrates to study how substratestiffness influences adhesion and proliferation. Collected images were analyzed for mitosis, adhesion, and filopodia-stretchability using ImageJ, an image processing program. Images were enhanced in order to perform cell counts at 24, 48, 72, and 96 hours of growth. Continuous recordings were produced to account for the number of mitosis occurrences and cellular migration on each of the substrates. Based on the conducted experiments, it appears that polished Ti-6Al-4V has a higher cell adherence than “nanopitted” anodized surface and an improved rate of proliferation which may be because the cells once adhered on the nano-pitted surface have less ability to detach in-order to undergo mitosis.https://engagedscholarship.csuohio.edu/u_poster_2014/1004/thumbnail.jp

Inappropriate antimicrobial use in Turkish pediatric hospitals: A multicenter point prevalence survey

Objectives: Although well-defined principles of rational antimicrobial use are available, inappropriate prescribing patterns are reported worldwide. Accurate information on the usage of antimicrobials, including factors associated with and influencing their use, is valuable for improving the quality of prescription practices. Methods: In this cross-sectional point prevalence survey, data on patients hospitalized in 12 different children's hospitals were collected on a single day. Appropriateness of prescription was compared between the types of antimicrobials prescribed, indications, wards, and presence of/consultation with an infectious disease physician (IDP). Results: A total 711 of 1302 (54.6%) patients evaluated were receiving one or more antimicrobial drugs. The antimicrobial prescription rate was highest in pediatric intensive care (75.7%) and lowest in the surgery wards (37.0%). Of the 711 patients receiving antimicrobials, 332 patients (46.7%) were found to be receiving at least one inappropriately prescribed drug. Inappropriate use was most frequent in surgery wards (80.2%), while it was less common in oncology wards (31.8%; p < 0.001). Respiratory tract infection was the most common indication for antimicrobial use (29.4%). Inappropriate use was more common in deep-seated infections (54.7%) and respiratory infections (56.5%). Fluoroquinolones were used inappropriately more than any other drugs (81.8%, p = 0.021). Consultation with an IDP appears to increase appropriate antimicrobial use (p = 0.008). Conclusions: Inappropriate antimicrobial use remains a common problem in Turkish pediatric hospitals. Consultation with an IDP and prescribing antimicrobial drugs according to microbiological test results could decrease the inappropriate use of antimicrobials

A Prospective Study of Etiology of Childhood Acute Bacterial Meningitis, Turkey

Vaccines to prevent bacterial meningitis in this region must provide reliable protection against serogroup W-135

Monoklonal antikor tekniği ile çocukluk çağı akut lenfoblastik lösemisi'nin immünolojik sınflandırılması

TEZ775Tez (Uzmanlık) -- Çukurova Üniversitesi, Adana, 1990.Kaynakça (s. 54-59) var.59 s. : rnk. res. ; 30 cm.

Bakteriyel, aseptik ve tüberküloz menejitli çocuklarda serum ve beyin-omurilik sıvısı (BOS) interlökin-6 (İL-6) ve interlökin-8 (İL-8) düzeyleri

TEZ1974Tez (Uzmanlık) -- Çukurova Üniversitesi, Adana, 1998.Kaynakça (s. 56-63) var.64 s. ; 30 cm.

Cerebrospinal fluid shunt infections and treatment

Serebrospinal şantlar hidrosefali yönetiminde önemli rol oynamaktadır. Serebrospinal sıvı şant enfeksiyonları, çocukluk döneminde serebrospinal enfeksiyonların önde gelen nedenlerinden birisidir ve uygun şekilde yönetilmez ise, artmış mortalite ve morbiditeyle sonuçlanabilir. Dört dekada yakın süredir modern valv kontrollü şantlar kullanılmasına rağmen, şant enfeksiyonlarının önlenmesi ve yönetimiyle ilgili pek çok soru bulunmaktadır. Bu yazıda serebrospinal sıvı şant enfeksiyonları risk faktörleri, tanı ve tedavi yöntemleri tartışılmıştır.Cerebrospinal fluid shunts play a major role in the management of hydrocephalus. Cerebrospinal fluid shunt infection is one of the leading causes of cerebrospinal infection in childhood and if not adequately managed, may result in increased mortality and morbidity. Despite the fact that the modern valve controlled shunt has been used for nearly four decades, many questions remain about the prevention and management of cerebrospinal fluid shunt infections. In this review, the risk factors, diagnosis and treatment of shunt infections were discussed

A difficult pathogen in pediatric infections: Enterococcus

Enterokoklar gram pozitif, düşük intrinsik virülanslı fakültatif bakterilerdir. Normal insan florasında kolonize oldukları için, kültürlerin klinikte dikkatli değerlendirilmesi, gereksiz ve potansiyel yan etkili antimikrobiyal kullanımdan kaçınmak için önemlidir. Ayrıca intrensek ve sonradan kazanılmış antibiyotik direncinden dolayı son yıllarda hastane enfeksiyonlarında sıklıkla izole edilmektedirler. Bu yazıda enterokokların genel özellikleri, çocuklardaki klinik tabloları ve tedavi yöntemleri anlatılmıştır.Enterococci are gram-positive, facultative bacteria with low intrinsic virulence. Since enterococci constitute part of the normal colonizing flora, careful clinical interpretation of cultures that grow enterococci is paramount to avoid unnecessary and potentially deleterious antimicrobial therapy. Due to the fact that they have intrinsic and acquired resistance to many antimicrobial agents, they have been one of the most frequently isolated microorganisms from hospital infection in recent years. In this article, enterococci bacteria, clinical characteristics in children and treatment were described

Incomplete Kawasaki disease: a pediatric diagnostic conflict - Reply

WOS: 000256807300017