Operational volcanic ash monitoring during Etna volcanic crises

Operational systems able to monitor volcanic ash in real time and provide both critical eruption parameters and useful warnings to emergency responders and government agencies should be implemented in most volcanic observatories worldwide. Over the past ten years, more than fifty lava fountains occurred at Mt. Etna (Italy) that produced eruption columns more than 10 km a.s.l. and generated large tephra fallout around the volcano flanks. For civil protection purposes, there was the need to improve the already existing monitoring systems daily run at the Istituto Nazionale di Geofisica and Vulcanologia, mainly based on eruption scenarios (weak and strong plume scenarios). We present a new upgraded system that has multiple objectives: i) to have a fast system able to best identify the type of eruptive scenario; ii) to forecast the tephra deposit in near real time, i.e. within a few hours from the eruptive event; iii) to determine the area impacted by clasts larger than 5 cm that could severely injure hikers, guides, and volcanologists and damage infrastructures in proximity of Etna summit craters. This new system is based on the real-time estimate of column height from the analysis of images taken by SEVIRI satellite and by new calibrated cameras and using meteorological parameters obtained by local models.PublishedVienna , Austria6V. Pericolosità vulcanica e contributi alla stima del rischi

Near-Real-Time Tephra Fallout Assessment at Mt. Etna, Italy

During explosive eruptions, emergency responders and government agencies need to make fast decisions that should be based on an accurate forecast of tephra dispersal and assessment of the expected impact. Here, we propose a new operational tephra fallout monitoring and forecasting system based on quantitative volcanological observations and modelling. The new system runs at the Istituto Nazionale di Geofisica e Vulcanologia, Osservatorio Etneo (INGV-OE) and is able to provide a reliable hazard assessment to the National Department of Civil Protection (DPC) during explosive eruptions. The new operational system combines data from low-cost calibrated visible cameras and satellite images to estimate the variation of column height with time and model volcanic plume and fallout in near-real-time(NRT). The new system has three main objectives: (i) to determine column height in NRT using multiple sensors (calibrated cameras and satellite images); (ii) to compute isomass and isopleth maps of tephra deposits in NRT; (iii) to help the DPC to best select the eruption scenarios run daily by INGV-OE every three hours. A particular novel feature of the new system is the computation of an isopleth map, which helps to identify the region of sedimentation of large clasts (≥5 cm) that could cause injuries to tourists, hikers, guides, and scientists, as well as damage buildings in the proximity of the summit craters. The proposed system could be easily adapted to other volcano observatories worldwide.Publishedid 29876V. Pericolosità vulcanica e contributi alla stima del rischioJCR Journa

A new way to reduce the impact from tephra fallout during Etna explosive eruptions

The frequent number of explosive events at Mt. Etna, in Italy, over the last ten years, has made necessary the improvement of volcanic ash monitoring and forecasting system at the Istituto Nazionale di Geofisica e Vulcanologia, Osservatorio Etneo (INGV-OE). Tephra fallout produced during Etna lava fountains largely impact the population living on the volcano flanks. In addition, during one of the most powerful paroxysms, large clasts fell in proximal areas injured tourists and hikers. To reduce risk, the Italian Department Civil Protection (DPC) asked and funded INGV-OE to do a research project finalized to three specific objectives. First, identify the plume scenario (i.e. weak plume scenario (WPS) and strong plume scenarios (SPS)) based on 1-D plume model. Second, forecast characteristics of tephra deposition using near real time observations. Third, identify the region possibly impacted by large clasts (>5 cm). Two algorithms were developed to measure the column height. One from the calibrated images of two visible cameras installed on the S and W flanks of the volcano, respectively; and the other one from satellite data using a procedure based on the computation of the volcanic plume-top brightness temperature at 10.8 mm. The analysis of lava fountains that occurred between 2011 and 2015 provided the opportunity to differentiate between weak, transitional and strong plumes. The uncertainty associated with eruption source parameters, while maintaining a fixed plume height, was also assessed. In the near future the implementation of these products into the INGV-OE - monitoring room will guarantee a better and timely information to civil protection authorities charged of risk prevention at different levels of responsibility.PublishedNapoli6V. Pericolosità vulcanica e contributi alla stima del rischi

Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis

Background: P64k is a Neisseria meningitidis high molecular weight protein present in meningococcal vaccine preparations. The lpdA gene, which encodes for this protein, was cloned in Escherichia coli and the P64k recombinant protein was expressed in E. coli K12 GC366 cells under the control of a tryptophan promoter. P64k was expressed as an intracellular soluble protein about 28% of the total cellular protein. Several scale-up criteria of fermentation processes were studied to obtain the recombinant P64k protein at the pilot production scale. Results: The best operational conditions at a larger scale production of P64k recombinant protein were studied and compared using the four following criteria: Constant Reynold's number (Re constant), Constant impeller tip speed (n di constant), Constant power consumption per unit liquid volume (P/V constant) and Constant volumetric oxygen transfer coefficients (KLa/k constant). The highest production of the recombinant protein was achieved based on the constant KLa/k scale-up fermentation criterion, calculating the aeration rate (Q) and the impeller agitation speed (n) by iterative process, keeping constant the KLa/k value from bench scale. The P64k protein total production at the 50 l culture scale was 546 mg l-1 in comparison with the 284 mg l-1 obtained at 1.5 l bench scale. Conclusions: The methodology described herein, for the KLa/k scale-up fermentation criterion, allowed us to obtain the P64k protein at 50 l scale. A fermentation process for the production of P64k protein from N. meningitidis was established, a protein to be used in future vaccine formulations in humans.How to cite: Espinosa R, García J, Narciandi E, et al. Scaling-up fermentation of Escherichia coli for production of recombinant P64k protein from Neisseria meningitidis. Electron J Biotechnol 2018;33. https://doi.org/10.1016/j.ejbt.2018.03.004. Keywords: Escherichia coli, Fermentation process, Fermentation, Gram negative diplococcus, High molecular weight protein, Meningococcal vaccine, Neisseria meningitidis, Obligate human pathogen, Recombinant P64k protein, Scale-up, Tryptophan promote

Tight glycemic control reduces heart inflammation and remodeling during acute myocardial infarction in hyperglycemic patients

Objectives: We analyzed the molecular mechanisms evoked by tight glycemic control during post-infarction remodeling in human hearts. Background: The molecular mechanisms by which tight glycemic control improves heart remodeling during acute myocardial infarction (AMI) are still largely unknown. Methods: Eighty-eight patients with first AMI undergoing coronary bypass surgery were studied: 38 normoglycemic patients served as the control group; hyperglycemic patients (glucose ≥140 mg/dl) were randomized to intensive glycemic control (IGC) (n = 25; glucose 80 to 140 mg/dl) or conventional glycemic control (CGC) (n = 25; glucose 180 to 200 mg/dl) for almost 3 days before surgery, with insulin infusion followed by subcutaneous insulin treatment. Echocardiographic parameters were investigated at admission and after treatment period. During surgery, oxidative stress (nitrotyrosine, superoxide anion [O2–] production, inducible nitric oxide synthase [iNOS]), inflammation (nuclear factor kappa B [NFκB], tumor necrosis factor [TNF]-α, and apoptosis (caspase-3) were analyzed in biopsy specimens taken from the peri-infarcted area. Results:Compared with normoglycemic patients, hyperglycemic patients had higher myocardial performance index (MPI) (p < 0.05), reduced ejection fraction (p < 0.05), more nitrotyrosine, iNOS, and O2– production, more macrophages, T-lymphocytes, and HLA-DR (Dako, Milan, Italy) cells, and more NFκB-activity, TNF-α, and caspase-3 levels (p < 0.01) in peri-infarcted specimens. After the treatment period, plasma glucose reduction was greater in the IGC than in the CGC group (p < 0.001). Compared with IGC patients, CGC patients had higher MPI (p < 0.02), had lower ejection fraction (p < 0.05), and had more markers of oxidative stress, more inflammation and apoptosis (p < 0.01) in peri-infarcted specimens. Conclusions: Tight glycemic control, by reducing oxidative stress and inflammation, might reduce apoptosis in peri-infarcted areas and remodeling in AMI patients