Assembly of Discrete Collagen–Chitosan Microenvironments into Multiphase Tissue Constructs

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/98229/1/673_ftp.pd

Design and Synthesis of Binding Growth Factors

Growth factors play important roles in tissue regeneration. However, because of their instability and diffusible nature, improvements in their performance would be desirable for therapeutic applications. Conferring binding affinities would be one way to improve their applicability. Here we review techniques for conjugating growth factors to polypeptides with particular affinities. Conjugation has been designed at the level of gene fusion and of polypeptide ligation. We summarize and discuss the designs and applications of binding growth factors prepared by such conjugation approaches

Selective Immobilization of Biomolecules on PTMC Network Surfaces Using Micro Contact Printing

Using poly(trimethylene carbonate) (PTMC) network surfaces containing unreacted macromer methacrylate groups, thiol-functionalized biotin (SH-undecyl-biotin) can be bound to the surface. With micro contact printing techniques and making use of the biotin-streptavidin (SAv) binding system patterned surfaces with biological functionality can be prepared in a very straightforward manner. This method has high potential in numerous biomedical applications