GSK3β-mediated DNA damage repair in pancreatic cancer

In this study we aimed to determine a deeper mechanistic understanding of GSK3β and its influence on pancreatic cancer behavior. Specifically, we examined the effect of GSK3β on transcriptional regulation and function of pancreatic cancer cells.2021-11-2

Accurate cosmic shear errors: do we need ensembles of simulations?

Accurate inference of cosmology from weak lensing shear requires an accurate shear power spectrum covariance matrix. Here, we investigate this accuracy requirement and quantify the relative importance of the Gaussian (G), super-sample covariance (SSC) and connected non-Gaussian (cNG) contributions to the covariance. Specifically, we forecast cosmological parameter constraints for future wide-field surveys and study how different covariance matrix components affect parameter bounds. Our main result is that the cNG term represents only a small and potentially negligible contribution to statistical parameter errors: the errors obtained using the G+SSC subset are within lesssim 5% of those obtained with the full G+SSC+cNG matrix for a Euclid-like survey. This result also holds for the shear two-point correlation function, variations in survey specifications and for different analytical prescriptions of the cNG term. The cNG term is that which is often tackled using numerically expensive ensembles of survey realizations. Our results suggest however that the accuracy of analytical or approximate numerical methods to compute the cNG term is likely to be sufficient for cosmic shear inference from the next generation of surveys

Genetic and serological heterogeneity of the supertypic HLA-B locus specificities Bw4 and Bw6

Gene cloning and sequencing of the HLA-B locus split antigens B38 (B16.1) and B39 (B16.2) allowed localization of their subtypic as well as their public specificities HLA-Bw4 or -Bw6 to the c~-helical region of the c~ 1 domain flanked by the amino acid positions 74-83. Comparison of their amino acid sequences with those of other HLA-B-locus alleles established HLA-Bw6 to be distinguished by Ser at residue 77 and Asn at residue 80. In contrast, HLA-Bw4 is characterized by at least seven different patterns of amino acid exchanges at positions 77 and 80-83. Reactivity patterns of Bw4- or Bw6-specific monoclonal antibodies reveal two alloantigenic epitopes contributing to the HLA-Bw4 or -Bw6 specificity residing next to the region of highest diversity of the cr domain

Identification and quantification of cannabinol as a biomarker for local hemp retting in an ancient sedimentary record by HPTLC-ESI-MS

Cannabis products have been used in various fields of everyday life for many centuries, and applications in folk medicine and textile production have been well-known for many centuries. For traditional textile production, hemp fibers were extracted from the stems by water retting in stagnant or slow-moving waters. During this procedure, parts of the plant material‚ among them phytocannabinoids‚ are released into the water. Cannabinol (CBN) is an important degradation product of the predominant phytocannabinoids found in Cannabis species. Thus, it is an excellent indicator for present as well as ancient hemp water retting. In this study, we developed and validated a simple and fast method for the determination of CBN in sediment samples using high-performance thin-layer chromatography (HPTLC) combined with electrospray ionization mass spectrometry (ESI-MS), thereby testing different extraction and cleanup procedures‚ as well as various sorbents and solvents for planar chromatography. This method shows a satisfactory overall analytical performance with an average recovery rate of 73%. Our protocol enabled qualitative and quantitative analyses of CBN in samples of a bottom sediment core‚ having been obtained from a small lake in Northern India, where intense local retting of hemp was suggested in the past. The analyses showed a maximum CBN content in pollen zone 4 covering a depth range of 262–209 cm, dating from approximately 480 BCE to 1050 CE. These findings correlate with existing records of Cannabis-type pollen. Thus, the method we propose is a helpful tool to track ancient hemp retting activities