Isopropyl 3-oxo-2,3-dihydro-1,2-benzothia­zole-2-carboxyl­ate

The title compound, C11H11NO3S, was synthesized by the reaction of benzo[d]isothia­zol-3(2H)-one with isopropanol in toluene. The benzoisothia­zolone ring system is essentially planar, with a mean deviation of 0.018 (2) Å from the least–squares plane defined by the nine constituent atoms. In the crystal, mol­ecules are linked by weak inter­molecular C—H⋯O hydrogen bonds

2-[(3-Bromo­anilino)meth­yl]-1,2-benzo­thia­zol-3(2H)-one

The title compound, C14H11BrN2OS, was synthesized by the reaction of 1,2-benzothia­zol-3(2H)-one with formalin and 3-bromo­aniline in ethanol. The 1,2-benzothia­zolone ring system is approximately planar [maximum deviation = 0.0142 (s.u.?) Å] and forms a dihedral angle of 79.19 (5)° with the benzene ring. In the crystal, molecules are linked by N—H⋯O, C—H⋯O and C—H⋯Br interactions

Ethyl 3-oxo-2,3-dihydro-1,2-benzothia­zole-2-carboxyl­ate

The title compound, C10H9NO3S, was synthesized by the reaction of benzo[d]isothia­zol-3(2H)-one with ethyl carbonochloridate in toluol. The benzisothia­zolone ring system is approximately planar, with a maximum deviation from the mean plane of 0.020 (1) Å for the N atom

Treatment of Leishmania (Leishmania) Amazonensis-Infected Mice with a Combination of a Palladacycle Complex and Heat-Killed Propionibacterium acnes Triggers Protective Cellular Immune Responses

Palladacycle complex DPPE 1.2 was previously reported to inhibit the in vitro and in vivo infection by Leishmania (Leishmania) amazonensis. The aim of the present study was to compare the effect of DPPE 1.2, in association with heat-killed Propionibacterium acnes, on L. (L.) amazonensis infection in two mouse strains, BALB/c and C57BL/6, and to evaluate the immune responses of the treated animals. Foot lesions of L. (L.) amazonensis-infected mice were injected with DPPE 1.2 alone, or associated with P. acnes as an adjuvant. Analysis of T-cell populations in the treated mice and in untreated controls was performed by FACS. Detection of IFN-gamma-secreting lymphocytes was carried out by an ELISPOT assay and active TGF-beta was measured by means of a double-sandwich ELISA test. The treatment with DPPE 1.2 resulted in a significant reduction of foot lesion sizes and parasite burdens in both mouse strains, and the lowest parasite burden was found in mice treated with DPPE 1.2 plus P. acnes. Mice treated with DPPE 1.2 alone displayed a significant increase of TCD4(+) and TCD8(+) lymphocytes and IFN-gamma secretion which were significantly higher in animals treated with DPPE 1.2 plus P. acnes. A significant reduction of active TGF-b was observed in mice treated with DPPE 1.2 alone or associated with P. acnes. Moreover, DPPE 1.2 associated to P. acnes was non-toxic to treated animals. The destruction of L. (L.) amazonensis by DPPE 1.2 was followed by host inflammatory responses which were exacerbated when the palladacycle complex was associated with P. acnes.Sao Paulo Research Foundation (FAPESP)FAPESPUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Farmacol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, Sao Paulo, BrazilUniv Fed Sao Paulo, Escola Paulista Med, Dept Farmacol, Sao Paulo, BrazilFAPESP: 2013/02133-8FAPESP: 2009/10809-6FAPESP: 2014/06935-4Web of Scienc