Produção de antissoro policlonal utilizando a proteína capsidial recombinante do Rupestris stem pitting-associated virus.

O Rupestris stem pitting-associated virus (RSPaV) é o agente causal das caneluras do lenho da videira. Este trabalho teve como objetivo produzir antissoro policlonal a partir da proteína capsidial (CP) recombinante do RSPaV e avaliar a sua especificidade e sensibilidade. O gene da CP do RSPaV, com 780pb, foi previamente caracterizado. Esse gene foi subclonado no sítio de restrição EcoRI, no vetor de expressão pRSET-B e o plasmídeo recombinante foi utilizado para induzir a expressão da CP em Escherichia coli. A CP, ligada a uma cauda de seis histidinas, foi purificada por meio de cromatografia de afinidade em coluna de Ni-NTA a partir do extrato de proteínas totais extraídas de E. coli. A identidade da proteína purificada foi confirmada em SDS-PAGE e Western blot, utilizando-se anticorpos comerciais contra a cauda de seis histidinas. A CP recombinante expressada in vitro apresentou massa molecular de cerca de 31kDa. A proteína purificada foi quantificada e 2,55mg foram utilizados para a imunização de um coelho. O antissoro policlonal obtido reagiu com diferentes isolados deste vírus, extraídos de videiras em ELISA indireto.Nota técnica

Room temperature nonlinear magnetoelectric effect in lead-free and Nb-doped AlFeO3 compositions

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFUNDAÇÃO ARAUCÁRIA DE APOIO AO DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO DO ESTADO DO PARANÁ - FAPPRCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORIt is still a challenging problem to obtain technologically useful materials displaying strong magnetoelectric coupling at room temperature. In the search for new effects and materials to achieve this kind of coupling, a nonlinear magnetoelectric effect was proposed in the magnetically disordered relaxor ferroelectric materials. In this context, the aluminum iron oxide (AlFeO3), a room temperature ferroelectric relaxor and magnetic spin glass compound, emerges as an attractive lead-free magnetoelectric material along with nonlinear magnetoelectric effects. In this work, static, dynamic, and temperature dependent ferroic and magnetoelectric properties in lead-free AlFeO3 and 2 at. Nb-doped AlFeO3 multiferroic magnetoelectric compositions are studied. Pyroelectric and magnetic measurements show changes in ferroelectric and magnetic states close to each other (∼200 K). The magnetoelectric coefficient behavior as a function of Hbias suggests a room temperature nonlinear magnetoelectric coupling in both single-phase and Nb-doped AlFeO3-based ceramic compositions.It is still a challenging problem to obtain technologically useful materials displaying strong magnetoelectric coupling at room temperature. In the search for new effects and materials to achieve this kind of coupling, a nonlinear magnetoelectric effect was proposed in the magnetically disordered relaxor ferroelectric materials. In this context, the aluminum iron oxide ( AlFeO3), a room temperature ferroelectric relaxor and magnetic spin glass compound, emerges as an attractive lead-free magnetoelectric material along with nonlinear magnetoelectric effects. In this work, static, dynamic, and temperature dependent ferroic and magnetoelectric properties in lead-free AlFeO3 and 2 at.% Nb-doped AlFeO3 multiferroic magnetoelectric compositions are studied. Pyroelectric and magnetic measurements show changes in ferroelectric and magnetic states close to each other ( similar to 200 K). The magnetoelectric coefficient behavior as a function of Hbias suggests a room temperature nonlinear magnetoelectric coupling in both single-phase and Nb-doped AlFeO3-based ceramic compositions.117617CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFUNDAÇÃO ARAUCÁRIA DE APOIO AO DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO DO ESTADO DO PARANÁ - FAPPRCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOFUNDAÇÃO ARAUCÁRIA DE APOIO AO DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO DO ESTADO DO PARANÁ - FAPPRCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR305129/2010-42008/04025-022825, 22870082/2007Hill, N.A., (2000) J. Phys. Chem. B, 104, p. 6694Ueda, K., Tabata, H., Kawai, T., (1999) Appl. Phys. Lett., 75, p. 555Kumar, M.M., Suresh, M.B., Suryanarayana, S.V., Kumar, G.S., Bhimasankaram, T., (1998) J. Appl. Phys., 84, p. 6811Dzyaloshinskii, I.E., Pitaevskii, L.P., (1959) Sov. Phys. JETP - USSR, 9, p. 1282Fiebig, M., (2005) J. Phys. D: Appl. Phys., 38, p. R123Freitas, V.F., Cotica, L.F., Santos, I.A., Garcia, D., Eiras, J.A., (2011) J. Eur. Ceram. Soc., 31, p. 2965Cotica, L.F., Estrada, F.R., Freitas, V.F., Dias, G.S., Santos, I.A., Eiras, J.A., Garcia, D., (2012) J. Appl. Phys., 111Gotardo, R.A.M., Cotica, L.F., Santos, I.A., Olzon-Dyonisio, M., Souza, S.D., Garcia, D., Eiras, J.A., Coelho, A.A., (2013) Appl. Phys. A: Mater. Sci. Process., 111, p. 563Lee, J.H., Fang, L., Vlahos, E., Ke, X.L., Jung, Y.W., Kourkoutis, L.F., Kim, J.W., Schlom, D.G., (2010) Nature, 466, p. 954Lorenz, B., Wang, Y.Q., Sun, Y.Y., Chu, C.W., (2004) Phys. Rev. B, 70Abe, N., Taniguchi, K., Ohtani, S., Umetsu, H., Arima, T., (2009) Phys. Rev. B, 80Kundys, B., Simon, C., Martin, C., (2008) Phys. Rev. B, 77Palkar, V.R., Purandare, S.C., Gohil, S., John, J., Bhattacharya, S., (2007) Appl. Phys. Lett., 90Kumar, A., Rivera, I., Katiyar, R.S., Scott, J.F., (2008) Appl. Phys. Lett., 92Pirc, R., Blinc, R., (2010) Ferroelectrics, 400, p. 387Cross, L.E., (1987) Ferroelectrics, 76, p. 241Dho, J., Kim, W.S., Hur, N.H., (2002) Phys. Rev. Lett., 89Cotica, L.F., De Medeiros, S.N., Santos, I.A., Paesano, A., Kinast, E.J., Da Cunha, J.B.M., Venet, M., Eiras, J.A., (2006) Ferroelectrics, 338, p. 241Devaux, X., Rousset, A., Broto, J.M., Rakoto, H., Askenazy, S., (1990) J. Mater. Sci. Lett., 9, p. 371Cotica, L.F., Santos, I.A., Venet, M., Garcia, D., Eiras, J.A., Coelho, A.A., (2008) Solid State Commun., 147, p. 123Santos, G.M., Silva, D.M., Freitas, V.F., Dias, G.S., Coelho, A.A., Pal, M., Santos, I.A., Bhalla, A.S., (2014) Ferroelectrics, 460, p. 108Bouree, F., Baudour, J.L., Elbadraoui, E., Musso, J., Laurent, C., Rousset, A., (1996) Acta Crystallogr., Sect. B: Struct. Sci., 52, p. 217Saha, R., Shireen, A., Shirodkar, S.N., Waghmare, U.V., Sundaresan, A., Rao, C.N.R., (2012) Solid State Commun., 152, p. 1964Shen, Y., Gao, J., Wang, Y., Finkel, P., Li, J., Viehland, D., (2013) Appl. Phys. Lett., 102Pirc, R., Blinc, R., (2009) Phys. Rev. B, 79Cotica, L.F., Zanatta, S.C., De Medeiros, S.N., Dos Santos, I.A., Paesano, A., Da Cunha, J.B.M., (2004) Solid State Ionics, 171, p. 283Yang, Y., Íñiguez, J., Mao, A.-J., Bellaiche, L., (2014) Phys. Rev. Lett., 112The authors would like to thank the Brazilian agencies CNPq (proc. 305129/2010-4), FAPESP (proc. 2008/04025-0), Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Paraná (Prots. 22825 and 22870), and CAPES (PROCAD 082/2007) for financial support. L.F.C. also acknowledge UTSA under the INAMM/NSF program for hosting him

Bˉ→Xsγ\bar{B}\to X_s \gamma in the Two Higgs Doublet Model up to Next-to-Next-to-Leading Order in QCD

We compute three-loop matching corrections to the Wilson coefficients $C_7$ and $C_8$ in the Two Higgs Doublet Model by applying expansions for small, intermediate and large charged Higgs boson masses. The results are used to evaluate the branching ratio of $\bar{B}\to X_s \gamma$ to next-to-next-to leading order accuracy, and to determine an updated lower limit on the charged Higgs boson mass. We find \mhplus \ge 380 GeV at 95% confidence level when the recently completed BABAR data analysis is taken into account. Our results for the charged Higgs contribution to the branching ratio exhibit considerably weaker sensitivity to the matching scale $\mu_0$, as compared to previous calculations.Comment: 20 pages, 15 figures; v2: minor modifications, matches published version in JHE

The monoclinic phase of PZT ceramics: Raman and phenomenological theory studies

This work reports on the first Raman detection of the tetragonal to monoclinic phase transition in PZT ceramics near morphotropic phase boundary at low temperatures. The transition is characterized by changes in the frequency of lattice modes with the temperature. The results presented here confirm the previous one recently reported by Noheda et al. using high-resolution synchrotron X-ray powder diffraction technique and dielectric measurements. The stability of the new phase is discussed within the framework of phenomenological Landau-Devonshire Theory.Comment: 6 pages including 4 figures, Latex, submitted to Applied Physics Letter

Reação de genótipos de feijão-caupi revela resistência às coinfecções pelo Cucumber mosaic virus, Cowpea aphid-borne mosaic virus e Cowpea severe mosaic virus.

O rendimento do feijão-caupi pode ser afetado por diversos fatores, em especial as viroses. As principais espécies de vírus que infectam o feijão-caupi, no Brasil, são: Cucumber mosaic virus (CMV), Cowpea aphid-borne mosaic virus (CABMV), Cowpea severe mosaic virus (CPSMV) e o Bean golden mosaic virus (BGMV). Este trabalho foi realizado em duas etapas e teve como objetivo avaliar a reação de genótipos de feijão-caupi quanto à resistência à infecção simples pelo CMV e mista nas combinações CMV+CABMV, CMV+CPSMV-I e MV+CABMV+CPSMV-I. Inicialmente, foram incluídos 57 genótipos, sendo três avaliações em gaiolas com tela antiafídeos sob infecção controlada, e uma em condição de campo sob infecção natural. Em seguida, foram selecionados 18 genótipos para serem desenvolvidos em nove ensaios, oito em gaiolas com tela antiafídeos sob infecção controlada, e um em campo sob infecção natural. Nesses ensaios, avaliaram-se os efeitos qualitativos e quantitativos resultantes das infecções. No ensaio de campo, foram avaliados o número de plantas assintomáticas, comprimento de vagem, número de grãos por vagem, massa de cem grãos e produtividade. As coinfecções reduziram a altura da planta e a massa seca. Além disso, nas infecções envolvendo os três vírus ocorreu a morte prematura de alguns genótipos. Os genótipos BR17-Gurguéia, Epace V-96, TE97-309G-9, TE97-309G-22, TE97-309G-24 e Patativa, além de bom comportamento diante das coinfecções virais, têm sementes com padrão comercial, podendo ser empregadas diretamente em programas de melhoramento

Involvement of patients or their representatives in quality management functions in EU hospitals:implementation and impact on patient-centred care strategies

OBJECTIVE: The objective of this study was to describe the involvement of patients or their representatives in quality management (QM) functions and to assess associations between levels of involvement and the implementation of patient-centred care strategies. DESIGN: A cross-sectional, multilevel STUDY DESIGN: that surveyed quality managers and department heads and data from an organizational audit. SETTING: Randomly selected hospitals (n = 74) from seven European countries (The Czech Republic, France, Germany, Poland, Portugal, Spain and Turkey). PARTICIPANTS: Hospital quality managers (n = 74) and heads of clinical departments (n = 262) in charge of four patient pathways (acute myocardial infarction, stroke, hip fracture and deliveries) participated in the data collection between May 2011 and February 2012. MAIN OUTCOME MEASURES: Four items reflecting essential patient-centred care strategies based on an on-site hospital visit: (1) formal survey seeking views of patients and carers, (2) written policies on patients' rights, (3) patient information literature including guidelines and (4) fact sheets for post-discharge care. The main predictors were patient involvement in QM at the (i) hospital level and (ii) pathway level. RESULTS: Current levels of involving patients and their representatives in QM functions in European hospitals are low at hospital level (mean score 1.6 on a scale of 0 to 5, SD 0.7), but even lower at departmental level (mean 0.6, SD 0.7). We did not detect associations between levels of involving patients and their representatives in QM functions and the implementation of patient-centred care strategies; however, the smallest hospitals were more likely to have implemented patient-centred care strategies. CONCLUSIONS: There is insufficient evidence that involving patients and their representatives in QM leads to establishing or implementing strategies and procedures that facilitate patient-centred care; however, lack of evidence should not be interpreted as evidence of no effect