Sorption competition and kinetics of trivalent cations (Eu, Y and Cm) on corundum (alpha-Al2O3 ) : A batch sorption and TRLFS study

Abstract In this study we have combined batch sorption and laser spectroscopic investigations to study the sorption of Eu(III) and Cm(III), on the aluminum oxide corundum in single- and multi-metal systems. Experiments were performed using a constant equilibrium time as a function of pH (pH-edges) or at constant pH as a function of equilibrium time (kinetic experiments) in 0.01 M NaClO4 and carbonate free conditions. The objective was to investigate how the sorption behavior of trivalent actinides and lanthanides is affected by the presence of another trivalent metal, Y(III). Our hypothesis was that the addition of higher concentrations of trivalent Y(III) together with a chemically similar trivalent metal, Eu(III) or Cm(III), would affect the sorption behavior of that metal. Batch experiments show that when the concentration of competing Y(III) is high enough (1 × 10−4 M) to occupy most of the surface sites, there is a clear shift in the position of the Eu(III) pH-edge to higher pH. Spectroscopic studies using time-resolved laser fluorescence spectroscopy (TRLFS) clearly confirm sorption competition between the trivalent metals Cm(III) and Y(III), but they also indicate a change in the surface speciation of the trivalent actinide in the presence of the competing metal if the concentration of that competing metal is high enough.Peer reviewe

Helicopter Location and Tracking using Seismometer Recordings

We use frequency domain methods usually applied to volcanic tremor to analyse ground based seismic recordings of a helicopter. We preclude misinterpretations of tremor sources and show alternative applications of our seismological methods. On a volcano, the seismic source can consist of repeating, closely spaced, small earthquakes. Interestingly, similar signals are generated by helicopters, due to repeating pressure pulses from the rotor blades. In both cases the seismic signals are continuous and referred to as tremor. As frequency gliding is in this case merely caused by the Doppler effect, not a change in the source, we can use its shape to deduce properties of the helicopter and its flight path. We show in this analysis that the number of rotor blades, rotor revolutions per minute (RPM), helicopter speed, flight direction, altitude and location can be deduced from seismometer recordings. Access to GPS determined flight path data from the helicopter offers us a robust way to test our location method

The effect of relationship status on communicating emotions through touch

Research into emotional communication to date has largely focused on facial and vocal expressions. In contrast, recent studies by Hertenstein, Keltner, App, Bulleit, and Jaskolka (2006) and Hertenstein, Holmes, McCullough, and Keltner (2009) exploring nonverbal communication of emotion discovered that people could identify anger, disgust, fear, gratitude, happiness, love, sadness and sympathy from the experience of being touched on either the arm or body by a stranger, without seeing the touch. The study showed that strangers were unable to communicate the self-focused emotions embarrassment, envy and pride, or the universal emotion surprise. Literature relating to touch indicates that the interpretation of a tactile experience is significantly influenced by the relationship between the touchers (Coan, Schaefer, & Davidson, 2006). The present study compared the ability of romantic couples and strangers to communicate emotions solely via touch. Results showed that both strangers and romantic couples were able to communicate universal and prosocial emotions, whereas only romantic couples were able to communicate the self-focused emotions envy and pride

Tremor-rich shallow dyke formation followed by silent magma flow at Bárdarbunga in Iceland

The Bárdarbunga eruption in Iceland in 2014 and 2015 produced about 1.6 km3 of lava. Magma propagated away from Bárdarbunga to a distance of 48 km in the sub-surface beneath Vatnajökull glacier, emerging a few kilometres beyond the glacier's northern rim. A puzzling observation is the lack of shallow (<3 km deep), high-frequency earthquakes associated with shallow dyke formation near the subaerial and subglacial eruptive sites, suggesting that near-surface dyke formation is seismically quiet. However, seismic array observations and seismic full wavefield simulations reveal the presence and nature of shallow, pre-eruptive, long-duration seismic tremor activity. Here we use analyses of seismic data to constrain the relationships between seismicity, tremor, dyke propagation and magma flow during the Bárðarbunga eruption. We show that although tremor is usually associated with magma flow in volcanic settings, pre-eruptive tremor at Bárdarbunga was probably caused by swarms of microseismic events during dyke formation, and hence is directly associated with fracturing of the upper 2–3 km of the crust. Subsequent magma flow in the newly formed shallow dyke was seismically silent, with almost a complete absence of seismicity or tremor. Hence, we suggest that the transition from temporarily isolated, large, deep earthquakes to many smaller, shallower, temporally overlapping earthquakes (< magnitude 2) that appear as continuous tremor announces the arrival of a dyke opening in the shallow crust, forming a pathway for silent magma flow to the Earth's surface

Micrometre-scale deformation observations reveal fundamental controls on geological rifting

Many of the world’s largest volcanic eruptions are associated with geological rifting where major fractures open at the Earth’s surface, yet fundamental controls on the near-surface response to the rifting process are lacking. New high resolution observations gleaned from seismometer data during the 2014 Bárðarbunga basaltic dyke intrusion in Iceland allow us unprecedented access to the associated graben formation process on both sub-second and micrometre scales. We find that what appears as quasi steady-state near-surface rifting on lower resolution GPS observation comprises discrete staccatolike deformation steps as the upper crust unzips through repetitive low magnitude (MW < 0) failures on fracture patches estimated between 300 m2 and 1200 m2 in size. Stress drops for these events are one to two orders of magnitude smaller than expected for tectonic earthquakes, demonstrating that the uppermost crust in the rift zone is exceptionally weak

Rottlerin stimulates apoptosis in pancreatic cancer cells through interactions with proteins of the Bcl-2 family

Rottlerin is a polyphenolic compound derived from Mallotus philipinensis. In the present study, we show that rottlerin decreased tumor size and stimulated apoptosis in an orthotopic model of pancreatic cancer with no effect on normal tissues in vivo. Rottlerin also induced apoptosis in pancreatic cancer (PaCa) cell lines by interacting with mitochondria and stimulating cytochrome c release. Immunoprecipitation results indicated that rottlerin disrupts complexes of prosurvival Bcl-xL with Bim and Puma. Furthermore, siRNA knockdown showed that Bim and Puma are necessary for rottlerin to stimulate apoptosis. We also showed that rottlerin and Bcl-2 and Bcl-xL inhibitor BH3I-2' stimulate apoptosis through a common mechanism. They both directly interact with mitochondria, causing increased cytochrome c release and mitochondrial depolarization, and both decrease sequestration of BH3-only proteins by Bcl-xL. However, the effects of rottlerin and BH3I-2' on the complex formation between Bcl-xL and BH3-only proteins are different. BH3I-2' disrupts complexes of Bcl-xL with Bad but not with Bim or Puma, whereas rottlerin had no effect on the Bcl-xL interaction with Bad. Also BH3I-2', but not rottlerin, required Bad to stimulate apoptosis. In conclusion, our results demonstrate that rottlerin has a potent proapoptotic and antitumor activity in pancreatic cancer, which is mediated by disrupting the interaction between prosurvival Bcl-2 proteins and proapoptotic BH3-only proteins. Thus rottlerin represents a promising novel agent for pancreatic cancer treatment

Transcriptomic and CRISPR/Cas9 technologies reveal FOXA2 as a tumor suppressor gene in pancreatic cancer

Pancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer with low survival rates and limited therapeutic options. Thus elucidation of signaling pathways involved in PDAC pathogenesis is essential for identifying novel potential therapeutic gene targets. Here, we used a systems approach to elucidate those pathways by integrating gene and microRNA profiling analyses together with CRISPR/Cas9 technology to identify novel transcription factors involved in PDAC pathogenesis. FOXA2 transcription factor was found to be significantly downregulated in PDAC relative to control pancreatic tissues. Functional experiments revealed that FOXA2 has a tumor suppressor function through inhibition of pancreatic cancer cell growth, migration, invasion, and colony formation. In situ hybridization analysis revealed miR-199a to be significantly upregulated in pancreatic cancer. Bioinformatics and luciferase analyses showed that miR-199a negatively but directly regulates FOXA2 expression through binding in its 3′-untranslated region (UTR). Evaluation of the functional importance of miR-199a on pancreatic cancer revealed that miR-199a acts as an inhibitor of FOXA2 expression, inducing an increase in pancreatic cancer cell proliferation, migration, and invasion. Additionally, gene ontology and network analyses in PANC-1 cells treated with a small interfering RNA (siRNA) against FOXA2 revealed an enrichment for cell invasion mechanisms through PLAUR and ERK activation. FOXA2 deletion (FOXA2Δ) by using two CRISPR/Cas9 vectors in PANC-1 cells induced tumor growth in vivo resulting in upregulation of PLAUR and ERK pathways in FOXA2Δ xenograft tumors. We have identified FOXA2 as a novel tumor suppressor in pancreatic cancer and it is regulated directly by miR-199a, thereby enhancing our understanding of how microRNAs interplay with the transcription factors to affect pancreatic oncogenesis