Temas Socio-Jurídicos. Volumen 27 No. 56 Junio de 2009

La revista Temas Socio-Jurídicos es una publicación seriada del Centro de Investigaciones Socio-Jurídicas, dependencia adscrita ala Facultad de Derecho de la Universidad Autónoma de Bucaramanga, que se dirige principalmente a abogados, profesionales de las ciencias sociales y humanas, a estudiantes de derecho y de ciencias sociales y humanas.The Socio-Legal Issues magazine is a serial publication of the Center for Socio-Legal Research, a dependency attached to the Faculty of Law of the Autonomous University of Bucaramanga, which is aimed mainly at lawyers, professionals in the social and human sciences, law students and social and human sciences

Incremento en las habilidades de análisis vocal en voz normal v/s patológica en estudiantes de fonoaudiología a través de un programa de entrenamiento

Tesis (Fonoaudiología)La discriminación auditiva es la capacidad que tiene el ser humano de crear una representación mental de un mensaje, mediante la identificación, interpretación y organización de la información acústica, codificada a través del oído. Evaluar el efecto de un entrenamiento sobre voces normales v/s patológicas en los alumnos de fonoaudiología que cursan cátedras del área de voz en el cuarto semestre en la Universidad Andrés Bello. La muestra correspondió a 24 alumnos que estaban cursando la asignatura fisiología de la voz de la carrera de fonoaudiología de la Universidad Andrés Bello, expuestos al “Programa de Entrenamiento de Análisis de Voces” (PEAV). El estudio comenzó con un diagnóstico de la habilidad de los alumnos para discriminar voces normales y/o patológicas y además los parámetros perceptuales de ellas. Posteriormente al entrenamiento auditivo se realizó una reevaluación para analizar resultados del inicio y fin del programa, y así ver el efecto que tuvo el éste. Se realizó una comparación con una prueba antes y después del PEAV donde se obtuvo un incremento estadísticamente significativo con respecto a la discriminación auditiva que presentaron los alumnos del cuarto semestre de fonoaudiología de la Universidad Andrés Bello, con respecto a la distinción de voz normal v/s patológica y los parámetros perceptuales que estas presentaban.Introduction: The auditory discrimination is the ability of the human being for creating a mental representation of a message, through the identification, interpretation and organisation of the acoustic information, encoded through the ear. Objective: To evaluate the effect of a training on normal voices and pathological voices in the speech therapy students who study lessons of the voice area in the fourth semester at the Andrés Bello University. Methodology: The sample corresponded of 24 students who were studying the course physiology of the voice in the speech therapy major at Andrés Bello University. The students were exposed to the "Voices Analysis Training Program" (PEAV). The study began with a diagnosis of the ability of students to discriminate normal and / or pathological voices and also the perceptual parameters of them. After the auditory training, a re-evaluation was performed to analyze the results of the start and end of the program, and thus see the effect that the program had. Results: A comparison was made with a test before and after the PEAV where a statistically significant increase was obtained in relation to the auditory discrimination presented by the students of the fourth semester of speech therapy at Andrés Bello University, regarding the distinction of normal voice pathological v / s the perceptual parameters that they presented. Conclusion: Although it is possible to find other platforms of mobile applications as support tools in the development of theoretical and practical skills in the area of voice and other areas, most are for theoretical use and diagnosis, but not for practical use as the PEAV is, which emerges as an unprecedented innovation in the practice of the area of the voice

Actividad anti-proliferativa de Aloysia sonorensis sobre líneas celulares de cáncer

Aloysia sonorensis, a perennial plant from the southern coast of Sonora, Mexico, known as “Mariola”, has been used to treat different diseases such as cold, fever and stomach aches. Furthermore, some species of the Aloysia genus have shown anti-proliferative activity against cancerous cell lines. Thus, the aim of the present work was to determine the anti-proliferative activity of Aloysia sonorensis extracts against cancerous cell lines and to identify the compounds responsible for such activity. The aerial parts were extracted with methanol which in turn was further fractionated with organic solvents. The methanolic extract and its fractions were screened for cytotoxic activity, using the MTT assay, against different cancerous cell lines. The FH12 subfraction displayed selective cytotoxicity with IC50 values of 2.84 and 12.12 µg/mL against RAW 264.7 and HeLa cancerous cell lines, respectively. Similarly, FH22 subfraction showed IC50 values of 6.32 and 13.87 µg/mL against M12.C3. F6 and HeLa cancerous cell lines, respectively. Moreover, the FAc1 displayed IC50 values of 9.42 (RAW 264.7) and 8.93 (M12.C3.F6) µg/mL. Piperitenone, a monoterpene, was identified during the bioactivity-guided fractionation.Aloysia sonorensis, planta perene de la costa sur de Sonora, México, conocida como “Mariola”, ha sido utilizada para el tratamiento de enfermedades como resfriado, fiebre y dolor de estómago. Además, algunas especies del género Aloysia han mostrado actividad antiproliferativa contra líneas celulares cancerosas. Debido a ésto, el objetivo del presente trabajo fue determinar la actividad antiproliferativa de Aloysia sonorensis contra líneas celulares cancerosas e identificar los compuestos responsables de esta actividad. Las partes aéreas de la planta se extrajeron con metanol, el cual fue fraccionado con solventes orgánicos. Se determinó la actividad antiproliferativa del extracto metanólico, y sus fracciones, contra diferentes líneas celulares cancerosas, usando el método del MTT. La subfracción FH12 mostró citotoxicidad selectiva con valores de IC50 de 2.84 y 12.12 µg/mL contra las líneas celulares cancerosas RAW 264.7 y HeLa, respectivamente. La subfracción FH22 mostró valores de IC50 de 6.32 y 13.87 µg/mL contra las líneas celulares cancerosas M12.C3.F6 y HeLa, respectivamente. La fracción FAc1 mostró valores de IC50 de 9.42 y 8.93 µg/mL contra las líneas celulares cancerosas RAW 264.7 y M12.C3.F6, respectivamente. Se determinó la presencia del monoterpeno Piperitenona durante el proceso de fraccionamiento biodirigido

Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats

Recently, soluble forms of aluminum for human use or consumption have been determined to be potentially toxic due to their association with hepatic, neurological, hematological, neoplastic, and bone conditions. This study aims to assess the genotoxic effect of aluminum chloride on genomic instability associated with the onset of N-nitroso-N-methylurea (NMU)-induced breast cancer in Sprague Dawley rats. The dietary behavior of the rats was assessed, and the concentration of aluminum in the mammary glands was determined using atomic absorption spectroscopy. Genomic instability was determined in the histological sections of mammary glands stained with hematoxylin and eosin. Moreover, micronucleus in peripheral blood and comet assays were performed. The results of dietary behavior evaluation indicated no significant differences between the experimental treatments. However, aluminum concentration in breast tissues was high in the +2000Al/−NMU treatment. This experimental treatment caused moderate intraductal cell proliferation, lymph node hyperplasia, and serous gland adenoma. Furthermore, micronucleus and comet test results revealed that +2000Al/−NMU led to a genotoxic effect after a 10-day exposure and the damage was more evident after a 15-day exposure. Therefore, in conclusion, genomic instability is present and the experimental conditions assessed are not associated with breast cancer

Assessment of plasma chitotriosidase activity, CCL18/PARC concentration and NP-C suspicion index in the diagnosis of Niemann-Pick disease type C : A prospective observational study

Niemann-Pick disease type C (NP-C) is a rare, autosomal recessive neurodegenerative disease caused by mutations in either the NPC1 or NPC2 genes. The diagnosis of NP-C remains challenging due to the non-specific, heterogeneous nature of signs/symptoms. This study assessed the utility of plasma chitotriosidase (ChT) and Chemokine (C-C motif) ligand 18 (CCL18)/pulmonary and activation-regulated chemokine (PARC) in conjunction with the NP-C suspicion index (NP-C SI) for guiding confirmatory laboratory testing in patients with suspected NP-C. In a prospective observational cohort study, incorporating a retrospective determination of NP-C SI scores, two different diagnostic approaches were applied in two separate groups of unrelated patients from 51 Spanish medical centers (n = 118 in both groups). From Jan 2010 to Apr 2012 (Period 1), patients with ≥2 clinical signs/symptoms of NP-C were considered 'suspected NP-C' cases, and NPC1/NPC2 sequencing, plasma chitotriosidase (ChT), CCL18/PARC and sphingomyelinase levels were assessed. Based on findings in Period 1, plasma ChT and CCL18/PARC, and NP-C SI prediction scores were determined in a second group of patients between May 2012 and Apr 2014 (Period 2), and NPC1 and NPC2 were sequenced only in those with elevated ChT and/or elevated CCL18/PARC and/or NP-C SI ≥70. Filipin staining and 7-ketocholesterol (7-KC) measurements were performed in all patients with NP-C gene mutations, where possible. In total across Periods 1 and 2, 10/236 (4%) patients had a confirmed diagnosis o NP-C based on gene sequencing (5/118 [4.2%] in each Period): all of these patients had two causal NPC1 mutations. Single mutant NPC1 alleles were detected in 8/236 (3%) patients, overall. Positive filipin staining results comprised three classical and five variant biochemical phenotypes. No NPC2 mutations were detected. All patients with NPC1 mutations had high ChT activity, high CCL18/PARC concentrations and/or NP-C SI scores ≥70. Plasma 7-KC was higher than control cut-off values in all patients with two NPC1 mutations, and in the majority of patients with single mutations. Family studies identified three further NP-C patients. This approach may be very useful for laboratories that do not have mass spectrometry facilities and therefore, they cannot use other NP-C biomarkers for diagnosis

II Congreso internacional en educación física, recreación, deporte y actividad física. “Innovación y tendencias” - Memorias

El II Congreso Internacional de Educación Física, Recreación, Deporte y Actividad Física: “Innovación y Tendencias“ y el II Encuentro de Egresados del Departamento de Cultura Física de la Universidad de Córdoba se realizó en la ciudad de Montería, capital del Departamento de Córdoba, Colombia, como iniciativa del Grupo de Investigación en Ciencias de la Actividad Fisica y la Salud –GICAFS- del Departamento de Cultura Física, perteneciente a la Facultad de Educación y Ciencias Humanas de la Universidad de Córdoba, como muestra hacia la comunidad académica y la sociedad en general de la responsabilidad ética, social y profesional, para aportar a la construcción de tejido social, atendiendo a las recomendaciones del plan decenal del deporte, la Educación Física, la Recreación y la Actividad Física 2009-2019 (COLDEPORTES, 2009).Edición 201