Extracting DNA of nematodes communities from Argentine Pampas agricultural soils

We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on agroecosystems

Fig. 5 in Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina

Fig. 5. Seasonal phenology of C. bridarollii on a pasture. Buenos Aires, Argentina. 1991–1993. Graph top: Total number of individuals, in all developmental stages, found in soil samples per mo.Published as part of <i>Lopez, Alicia N., Alvarez Castillo, Hector A., Carmona, Dora, Manetti, Pablo L., Mondino, Eduardo & Vincini, Ana M., 2002, Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina, pp. 259-269 in The Coleopterists Bulletin 56 (2)</i> on page 267, DOI: 10.1649/0010-065X(2002)056[0259:BAOCBB]2.0.CO;2, <a href="http://zenodo.org/record/10103159">http://zenodo.org/record/10103159</a&gt

Fig. 4 in Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina

Fig. 4. Number of eggs/d laid by C. bridarollii and mean incubation period.Published as part of <i>Lopez, Alicia N., Alvarez Castillo, Hector A., Carmona, Dora, Manetti, Pablo L., Mondino, Eduardo & Vincini, Ana M., 2002, Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina, pp. 259-269 in The Coleopterists Bulletin 56 (2)</i> on page 266, DOI: 10.1649/0010-065X(2002)056[0259:BAOCBB]2.0.CO;2, <a href="http://zenodo.org/record/10103159">http://zenodo.org/record/10103159</a&gt

Fig. 6 in Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina

Fig. 6. Phenology of C. bridarollii on potato crop. Buenos Aires, Argentina. 1991–Published as part of <i>Lopez, Alicia N., Alvarez Castillo, Hector A., Carmona, Dora, Manetti, Pablo L., Mondino, Eduardo & Vincini, Ana M., 2002, Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina, pp. 259-269 in The Coleopterists Bulletin 56 (2)</i> on page 267, DOI: 10.1649/0010-065X(2002)056[0259:BAOCBB]2.0.CO;2, <a href="http://zenodo.org/record/10103159">http://zenodo.org/record/10103159</a&gt

Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina

Lopez, Alicia N., Alvarez Castillo, Hector A., Carmona, Dora, Manetti, Pablo L., Mondino, Eduardo, Vincini, Ana M. (2002): Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina. The Coleopterists Bulletin 56 (2): 259-269, DOI: 10.1649/0010-065X(2002)056[0259:BAOCBB]2.0.CO;2, URL: http://dx.doi.org/10.1649/0010-065x(2002)056[0259:baocbb]2.0.co;

Fig. 3 in Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina

Fig. 3. Age­specific survivorship of adult females of C. bridarollii and age­specific reproduction. A. N = 25, B. N = 21, C. N = 41Published as part of <i>Lopez, Alicia N., Alvarez Castillo, Hector A., Carmona, Dora, Manetti, Pablo L., Mondino, Eduardo & Vincini, Ana M., 2002, Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina, pp. 259-269 in The Coleopterists Bulletin 56 (2)</i> on page 265, DOI: 10.1649/0010-065X(2002)056[0259:BAOCBB]2.0.CO;2, <a href="http://zenodo.org/record/10103159">http://zenodo.org/record/10103159</a&gt

Fig. 2 in Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina

Fig. 2. Age­specific survivorship of adults (males and females) of C. bridarollii. A. N = 50, B. N = 42, C. N = 82.Published as part of <i>Lopez, Alicia N., Alvarez Castillo, Hector A., Carmona, Dora, Manetti, Pablo L., Mondino, Eduardo & Vincini, Ana M., 2002, Biological Aspects Of Colaspis Bridarollii (Bechyné) (Coleoptera: Chrysomelidae: Eumolpinae) In Argentina, pp. 259-269 in The Coleopterists Bulletin 56 (2)</i> on page 264, DOI: 10.1649/0010-065X(2002)056[0259:BAOCBB]2.0.CO;2, <a href="http://zenodo.org/record/10103159">http://zenodo.org/record/10103159</a&gt

Organic fertilization influences nematode diversity and maturity index in coffee tree plantations using an agroforestry system

In conventional coffee farming, soil fauna can be negatively affected by the intensive management practices adopted and the use of an agroforestry system (AFS) is an alternative to reduce these impacts. In coffee AFS, soil nutrition is provided mainly using organic fertilizers. This soil management favors the microbiota and can alter the population dynamics of some organisms. Our objective was to evaluate the effect of organic fertilizers on the nematode community in coffee AFS and to determine their impact on soil ecology. Soil samples were collected from three coffee AFS and a nearby Atlantic rainforest fragment. Nematodes were extracted from the samples and identified to the genus. The identified populations were compared using several community and diversity indices to determine the environmental conditions of the systems under evaluation. No differences in total abundance among nematode communities were found in the four areas evaluated. Regarding trophic groups, the coffee AFS treated with either cow manure or poultry litter favored the trophic group of bacterivores. Plant-parasitic nematodes were more abundant in soils of both the naturally fertilized coffee AFS and the Atlantic rainforest fragment. The maturity and structural indexes indicated that the Atlantic rainforest fragment and the naturally fertilized coffee AFS had similar ecological functions. On the other hand, soils fertilized with cow manure were less diverse, had higher dominance in the community, and showed less ecological stability. The nematode communities found in the AFS were similar to those seen in the forest fragment indicating that is possible to produce coffee sustainably without negatively affecting soil quality.Peer reviewe

Soil, litter, macrofauna and nematode data from Berenstecher et al. 2016

This data file contains unanalysed data from soil and litter characteristics, macrofaunal abundance and composition, nematode abundance and decomposition, litter and soil enzymatic activities and litter decomposition at sampling points before and after the massive Puyehue volcanic eruption of June 2011 in Patagonia, Argentin

Integrating the Internship into Ophthalmology Residency Programs: Association of University Professors of Ophthalmology American Academy of Ophthalmology White Paper

Future ophthalmologists will need to have broad skills to thrive in complex health care organizations. However, training for ophthalmologists does not take advantage of all of the postgraduate years (PGYs). Although the traditional residency years seem to have little excess capacity, enhancing the internship year does offer an opportunity to expand the time for ophthalmology training in the same 4 PGYs. Integrating the internship year into residency would allow control of all of the PGYs, allowing our profession to optimize training for ophthalmology. In this white paper, we propose that we could capture an additional 6 months of training time by integrating basic ophthalmology training into the intern year. This would allow 6 additional months to expand training in areas such as quality improvement or time for "mini-fellowships" to allow graduates to develop a deeper set of skills