Sobre grupos finitos admitindo um grupo de Frobenius como grupo de automorfismos

Dissertação (mestrado)—Universidade de Brasília, Instituto de Ciências Exatas, Departamento de Matemática, 2019.Esta dissertação apresenta um estudo sobre grupos finitos admitindo um grupo de Frobenius como grupo de automorfismos. Apresentamos as demonstrações de resultados inspirados nos problemas 17.72 e 18.67, propostos no The Kourovka Notebook, que tratam sobre a limitação da classe de nilpotência e do expoente de um grupo G, respectivamente. Além disso, apresentamos resultados importantes para Teoria de anéis de Lie, por exemplo, o Teorema de Higman-Kreknin-Krostrikin.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).This dissertation presents a study about finite groups admitting a group of Frobenius as its group of automorphisms with emphasis on questions about nilpotency and group exponent. We present the solutions of results inspired by problems 17.72 and 18.67, proposed in ‘The Kourovka Notebook’ these problems deals with limitations for nilpotency class and the exponent of a group G, respectively. Moreover, we study important results from Lie Ring Theory for instance the theorem of Higman-Kreknin-Krostrikin whose proof is found in this work

Impressões digitais de DNA e RNA através de AP-PCR em Entamoeba histolytica

Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.Diferenças na expressão gênica de cepas de E. histolytica foram obtidas pelo "fingerprinting" de RNA (RAP-PCR) e DNA (RAPD). A análise do perfil eletroforético do gel revelou alguns marcadores polimórficos que poderiam ser usados na caracterização individual das cepas. As 260 bandas geradas pela utilização de cinco primers diferentes, tanto no RAP-PCR, quanto no RAPD foram empregadas na construção de dendogramas. O dendograma obtido com os produtos do RAPD permitiu a distinção das cepas isoladas de pacientes sintomáticos e assintomáticos, além de correlacionar o polimorfismo exibido com a virulência das mesmas. O dendograma obtido com os produtos do RAP-PCR não apresentou correlação com a virulência das cepas, mas revelou uma exuberante variabilidade transcricional intra-específica, que pode estar relacionada a outros caracteres biológicos envolvidos, ou não, na patogênese da amebíase

Impressões digitais de DNA e RNA através de AP-PCR em Entamoeba histolytica

Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.Diferenças na expressão gênica de cepas de E. histolytica foram obtidas pelo "fingerprinting" de RNA (RAP-PCR) e DNA (RAPD). A análise do perfil eletroforético do gel revelou alguns marcadores polimórficos que poderiam ser usados na caracterização individual das cepas. As 260 bandas geradas pela utilização de cinco primers diferentes, tanto no RAP-PCR, quanto no RAPD foram empregadas na construção de dendogramas. O dendograma obtido com os produtos do RAPD permitiu a distinção das cepas isoladas de pacientes sintomáticos e assintomáticos, além de correlacionar o polimorfismo exibido com a virulência das mesmas. O dendograma obtido com os produtos do RAP-PCR não apresentou correlação com a virulência das cepas, mas revelou uma exuberante variabilidade transcricional intra-específica, que pode estar relacionada a outros caracteres biológicos envolvidos, ou não, na patogênese da amebíase

Impressões digitais de DNA e RNA através de AP-PCR em Entamoeba histolytica

Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.Diferenças na expressão gênica de cepas de E. histolytica foram obtidas pelo "fingerprinting" de RNA (RAP-PCR) e DNA (RAPD). A análise do perfil eletroforético do gel revelou alguns marcadores polimórficos que poderiam ser usados na caracterização individual das cepas. As 260 bandas geradas pela utilização de cinco primers diferentes, tanto no RAP-PCR, quanto no RAPD foram empregadas na construção de dendogramas. O dendograma obtido com os produtos do RAPD permitiu a distinção das cepas isoladas de pacientes sintomáticos e assintomáticos, além de correlacionar o polimorfismo exibido com a virulência das mesmas. O dendograma obtido com os produtos do RAP-PCR não apresentou correlação com a virulência das cepas, mas revelou uma exuberante variabilidade transcricional intra-específica, que pode estar relacionada a outros caracteres biológicos envolvidos, ou não, na patogênese da amebíase

Impressões digitais de DNA e RNA através de AP-PCR em Entamoeba histolytica

Differences were detected in the gene expression of strains of E. histolytica using RNA (RAP-PCR) and DNA fingerprinting (RAPD). Analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. The 260 bands generated by using five different primers for RAP-PCR, as well as RAPD, were employed in the construction of dendograms. The dendogram obtained based on the RAPD products permitted the distinction of symptomatic and asymptomatic isolates, as well the correlation between the polymorphism exhibited and the virulence of the strains. The dendogram obtained for the RAP-PCR products did not show a correlation with the virulence of the strains but revealed a high degree of intraspecific transcriptional variability that could be related to other biological features, whether or not these are involved in the pathogenesis of amebiasis.Diferenças na expressão gênica de cepas de E. histolytica foram obtidas pelo "fingerprinting" de RNA (RAP-PCR) e DNA (RAPD). A análise do perfil eletroforético do gel revelou alguns marcadores polimórficos que poderiam ser usados na caracterização individual das cepas. As 260 bandas geradas pela utilização de cinco primers diferentes, tanto no RAP-PCR, quanto no RAPD foram empregadas na construção de dendogramas. O dendograma obtido com os produtos do RAPD permitiu a distinção das cepas isoladas de pacientes sintomáticos e assintomáticos, além de correlacionar o polimorfismo exibido com a virulência das mesmas. O dendograma obtido com os produtos do RAP-PCR não apresentou correlação com a virulência das cepas, mas revelou uma exuberante variabilidade transcricional intra-específica, que pode estar relacionada a outros caracteres biológicos envolvidos, ou não, na patogênese da amebíase

The role of aquatic ecosystems (River Tua, Portugal) as reservoirs of multidrug-resistant Aeromonas spp.

The inappropriate use of antibiotics, one of the causes of the high incidence of antimicrobialresistant bacteria isolated from aquatic ecosystems, represents a risk for aquatic organisms and the welfare of humans. This study aimed to determine the antimicrobial resistance rates among riverine Aeromonas spp., taken as representative of the autochthonous microbiota, to evaluate the level of antibacterial resistance in the Tua River (Douro basin). The prevalence and degree of antibiotic resistance was examined using motile aeromonads as a potential indicator of antimicrobial susceptibility for the aquatic environment. Water samples were collected from the middle sector of the river, which is most impacted area by several anthropogenic pressures. Water samples were plated on an Aeromonas-selective agar, with and without antibiotics. The activity of 19 antibiotics was studied against 30 isolates of Aeromonas spp. using the standard agar dilution susceptibility test. Antibiotic resistance rates were fosfomycin (FOS) 83.33%, nalidixic acid (NA) 60%, cefotaxime (CTX) 40%, gentamicin (CN) 26.67%, tobramycin (TOB) 26.67%, cotrimoxazole (SXT) 26.67%, chloramphenicol (C) 16.67%, and tetracycline (TE) 13.33%. Some of the nalidixic acid-resistant strains were susceptible to fluoroquinolones. Multiple resistance was also observed (83.33%). The environmental ubiquity, the natural susceptibility to antimicrobials and the zoonotic potential of Aeromonas spp. make them optimal candidates for studying antimicrobial resistance (AMR) in aquatic ecosystems. Aquatic environments may provide an ideal setting for the acquisition and dissemination of antibiotic resistance because anthropogenic activities frequently impact them. The potential risk of multi- and pan-resistant bacteria transmission between animals and humans should be considered in a “One Health-One World” concept.This research was supported by the Portuguese Foundation for Science and Technology (FCT) and the Operational Competitiveness Programme (COMPETE), under the projectsUIDB/04033/2020 (CITAB-UTAD) and the UIDB/00690/2020 (CIMO-IPB).info:eu-repo/semantics/publishedVersio

Trabalhadores da Albras e sua organização política

As of the late 20th century and beginning of the 21st century, workers organizations have deeply changed. Workers struggle to defend their interests and those of the society in general have become more and more difficult. This study analyzes the particularities of the present status of Albras workers political organization, based on the changes undergone by the company, and how those changes affected the Metal Industry Workers Union of the State of Pará (SIMETAL). It tries to understand the challenges to the workers collective actions towards a new structure within the industrial capitalism.As formas organizativas de trabalho nas empresas vêm sofrendo profundas mudanças durante o final do século XX e início do século XXI, dificultando a luta em torno dos interesses dos trabalhadores e da sociedade em geral. Neste contexto, o objetivo deste estudo é analisar as particularidades da situação atual na organização política dos trabalhadores da Albras, a partir das transformações ocorridas nesta empresa, e de que forma repercute no Sindicato dos Trabalhadores nas Indústrias Metalúrgicas do Estado do Pará-SIMETAL. Deste modo, pretende-se compreender os desafios postos para a ação coletiva dos trabalhadores diante de uma nova reestruturação dentro do capitalismo industrial

Effects of Quercetin in transcriptional and post-transcriptional regulation of fetal hemoglobin

This project was supported by Instituto Politécnico de Lisboa under the grant IDI&CAIPL/2022/miRCa/ESTeSL and partially supported by FCT/MCTES (UIDB/05608/2020 and UIDP/05608/2020). The authors are grateful to Fernando Nunes for kindly providing the Carica papaya leaves.Hemoglobinopathies are a group of inherited blood disorders that primarily affect red blood cells. The most common type is known as sickle cell anemia (SCA). It is characterized by mutations in the HBB gene, which encodes the β-subunit of human hemoglobin, giving rise to hemoglobin S (HbS). When deoxygenated, HbS polymerizes in the red blood cell, giving it a sickle shape and making it rigid and fragile. Fetal hemoglobin (HbF) is the major genetic modulator of the hematologic and clinical features of sickle cell disease, an effect mediated by its exclusion from the sickle hemoglobin polymer. Fetal hemoglobin genes are genetically regulated, and the level of HbF and its distribution among sickle erythrocytes is highly variable. Currently, therapies that induce HbF are promising, such as hydroxyurea (HU). However, due to high costs for underdeveloped countries and the adverse side effects, it is important to test alternative products and develop new compounds, such as Quercetin, a natural flavonoid present in plants that has antioxidant and anti-inflammatory properties.info:eu-repo/semantics/publishedVersio

Effects of methanolic and aqueous Carica Papaya leaf extracts in transcriptional and miRNA-mediated regulation of fetal hemoglobin

This project was supported by Instituto Politécnico de Lisboa under the grant IDI&CA-IPL/2022/miRCa/ESTeSL and partially supported by FCT/MCTES (UIDB/05608/2020 and UIDP/05608/2020). The authors are grateful to Fernando Nunes for kindly providing the Carica Papaya leaves.Sickle cell disease (SCD) is a genetic blood disorder caused by mutations in β-globin gene that affect the shape and transport of red blood cells in blood vessels, leading to various clinical complications. The pharmacological reactivation of Fetal Hemoglobin (HbF) through compounds such as Hydroxyurea (HU), is one of the available treatments, however, their safety concerns and expensive cost in low- and middle-income countries limit their use. In this context, it is essential to study novel HbF-inducing compounds that have scarcer adverse effects and can be widely available, such as Carica papaya extracts, a medicinal plant with anti-oxidant and anti-inflammatory properties. Therefore, the main aim of this work is to evaluate the effects of Carica Papaya leaf extracts (CPLE) in HbF reactivation. More specifically, we started by evaluating the effect of a methanolic CPLE extract in K562 cells (human immortalized myeloid leukemia cell line) at the proliferation rate and viability of the K562 cell line, compared to HU exposure. Subsequently, we analyzed the expression levels of HBG1 and HBG2 genes and that of their transcriptional and miRNA-mediated regulators. To achieve these goals, the K562 cell line was first exposed for 72 hours to CPMLE at 500 μg/mL and for 24 hours to EMFCP (0.5; 50, and 100 μg/mL) and to HU (25 μg/mL). After exposure to natural compounds, the effects of gene expression were quantified from total RNA using RT-qPCR. The results have indicated that cell proliferation and viability were affected by CPMLE only at the concentration of 500 μg/ml, with no effects being observed at the lower concentrations analyzed. Upon analysis of the expression levels of globins (HBA, HBB, HBG1, and HBG2), HbF regulatory genes (MYB, KLF1, BCL11A, and BGLT3), and miRNAs involved in the regulation of HbF we could observe more significant differences for the lower concentrations of extracts used, namely at the concentration of 0.5 μg/ml. As such we have decided to titrate down the concentration of methanolic leaf extracts used and test the exposure to aqueous leaf extracts which possess different biological compounds that might lead to a differential regulation of the genes under analysis. Our preliminary results have revealed that at the concentrations of 0,05 ug/ml; 0,5 ug/ml and 5 ug/ml the cell viability and proliferation rates were not affected either for methanolic or aqueous extracts. We are currently analyzing the expression levels of target and regulatory genes to determine the effect of both types of leaf extracts on regulators of HbF expression.info:eu-repo/semantics/publishedVersio