Discovery of a new Transient X-ray Pulsar in the Small Magellanic Cloud

Rossi X-Ray Timing Explorer observations of the Small Magellanic Cloud have revealed a previously unknown transient X-ray pulsar with a pulse period of 95s. Provisionally designated XTE SMC95, the pulsar was detected in three Proportional Counter Array observations during an outburst spanning 4 weeks in March/April 1999. The pulse profile is double peaked reaching a pulse fraction \~0.8. The source is proposed as a Be/neutron star system on the basis of its pulsations, transient nature and characteristically hard X-ray spectrum. The 2-10 keV X-ray luminosity implied by our observations is > 2x10^37 erg/s which is consistent with that of normal outbursts seen in Galactic systems. This discovery adds to the emerging picture of the SMC as containing an extremely dense population of transient high mass X-ray binaries.Comment: Accepted by A&A. 7 pages, 6 figure

Reverberation Mapping and the Physics of Active Galactic Nuclei

Reverberation-mapping campaigns have revolutionized our understanding of AGN. They have allowed the direct determination of the broad-line region size, enabled mapping of the gas distribution around the central black hole, and are starting to resolve the continuum source structure. This review describes the recent and successful campaigns of the International AGN Watch consortium, outlines the theoretical background of reverberation mapping and the calculation of transfer functions, and addresses the fundamental difficulties of such experiments. It shows that such large-scale experiments have resulted in a ``new BLR'' which is considerably different from the one we knew just ten years ago. We discuss in some detail the more important new results, including the luminosity-size-mass relationship for AGN, and suggest ways to proceed in the near future.Comment: Review article to appear in Astronomical Time Series, Proceedings of the Wise Observatory 25th Ann. Symposium. 24 pages including 7 figure

Statistical analyses of long-term variability of AGN at high radio frequencies

We present a study of variability time scales in a large sample of Active Galactic Nuclei at several frequencies between 4.8 and 230 GHz. We investigate the differences of various AGN types and frequencies and correlate the measured time scales with physical parameters such as the luminosity and the Lorentz factor. Our sample consists of both high and low polarization quasars, BL Lacertae objects and radio galaxies. The basis of this work is the 22 GHz, 37 GHz and 87 GHz monitoring data from the Metsahovi Radio Observatory spanning over 25 years. In addition,we used higher 90 GHz and 230 GHz frequency data obtained with the SEST-telescope between 1987 and 2003. Further lower frequency data at 4.8 GHz, 8 GHz and 14.5 GHz from the University of Michigan monitoring programme have been used. We have applied three different statistical methods to study the time scales: The structure function, the discrete correlation function and the Lomb-Scargle periodogram. We discuss also the differences and relative merits of these three methods. Our study reveals that smaller flux density variations occur in these sources on short time scales of 1-2 years, but larger outbursts happen quite rarely, on the average only once in every 6 years. We do not find any significant differences in the time scales between the source classes. The time scales are also only weakly related to the luminosity suggesting that the shock formation is caused by jet instabilities rather than the central black hole.Comment: 19 pages, 12 figures, Accepted for publication in A&

Observational evidence for matter propagation in accretion flows

We study simultaneous X-ray and optical observations of three intermediate polars EX Hya, V1223 Sgr and TV Col with the aim to understand the propagation of matter in their accretion flows. We show that in all cases the power spectra of flux variability of binary systems in X-rays and in optical band are similar to each other and the majority of X-ray and optical fluxes are correlated with time lag <1 sec. These findings support the idea that optical emission of accretion disks, in these binary systems,largely originates as reprocessing of X-ray luminosity of their white dwarfs. In the best obtained dataset of EX Hya we see that the optical lightcurve unambiguously contains some component, which leads the X-ray emission by ~7 sec. We interpret this in the framework of the model of propagating fluctuations and thus deduce the time of travel of the matter from the innermost part of the truncated accretion disk to the white dwarf surface. This value agrees very well with the time expected for matter threaded onto the magnetosphere of the white dwarf to fall to its surface. The datasets of V1223 Sgr and TV Col in general confirm these findings,but have poorer quality.Comment: 7 pages, 6 figures. Accepted for publication in MNRA

Managing the complexity of doing it all : an exploratory study on students' experiences when trained stepwise in conducting consultations

Background: At most medical schools the components required to conduct a consultation, medical knowledge, communication, clinical reasoning and physical examination skills, are trained separately. Afterwards, all the knowledge and skills students acquired must be integrated into complete consultations, an art that lies at the heart of the medical profession. Inevitably, students experience conducting consultations as complex and challenging. Literature emphasizes the importance of three didactic course principles: moving from partial tasks to whole task learning, diminishing supervisors' support and gradually increasing students' responsibility. This study explores students' experiences of an integrated consultation course using these three didactic principles to support them in this difficult task. Methods: Six focus groups were conducted with 20 pre-clerkship and 19 clerkship students in total. Discussions were audiotaped, transcribed and analysed by Nvivo using the constant comparative strategy within a thematic analysis. Results: Conducting complete consultations motivated students in their learning process as future physician. Initially, students were very much focused on medical problem solving. Completing the whole task of a consultation obligated them to transfer their theoretical medical knowledge into applicable clinical knowledge on the spot. Furthermore, diminishing the support of a supervisor triggered students to reflect on their own actions but contrasted with their increased appreciation of critical feedback. Increasing students' responsibility stimulated their active learning but made some students feel overloaded. These students were anxious to miss patient information or not being able to take the right decisions or to answer patients' questions, which sometimes resulted in evasive coping techniques, such as talking faster to prevent the patient asking questions. Conclusion: The complex task of conducting complete consultations should be implemented early within medical curricula because students need time to organize their medical knowledge into applicable clinical knowledge. An integrated consultation course should comprise a step-by-step teaching strategy with a variety of supervisors' feedback modi, adapted to students' competence. Finally, students should be guided in formulating achievable standards to prevent them from feeling overloaded in practicing complete consultations with simulated or real patients

Targeted online liquid chromatography electron capture dissociation mass spectrometry for the localization of sites of in vivo phosphorylation in human Sprouty2

We demonstrate a strategy employing collision-induced dissociation for phosphopeptide discovery, followed by targeted electron capture dissociation (ECD) for site localization. The high mass accuracy and low background noise of the ECD mass spectra allow facile sequencing of coeluting isobaric phosphopeptides, with up to two isobaric phosphopeptides sequenced from a single mass spectrum. In contrast to the previously described neutral loss of dependent ECD method, targeted ECD allows analysis of both phosphotyrosine peptides and lower abundance phosphopeptides. The approach was applied to phosphorylation analysis of human Sprouty2, a regulator of receptor tyrosine kinase signaling. Fifteen sites of phosphorylation were identified, 11 of which are novel

Expanding the Repertoire of Natural Product-Inspired Ring Pairs for Molecular Recognition of DNA

A furan amino acid, inspired by the recently discovered proximicin natural products, was incorporated into the scaffold of a DNA-binding hairpin polyamide. While unpaired oligomers of 2,4-disubstituted furan amino acids show poor DNA-binding activity, furan (Fn) carboxamides paired with N-methylpyrrole (Py) and N-methylimidazole (Im) rings demonstrate excellent stabilization of duplex DNA as well as discrimination of noncognate sequences, consistent with function as a Py mimic according to the Py/Im polyamide pairing rules

Full-Stokes polarimetry with circularly polarized feeds - Sources with stable linear and circular polarization in the GHz regime

We present a pipeline that allows recovering reliable information for all four Stokes parameters with high accuracy. Its novelty relies on the treatment of the instrumental effects already prior to the computation of the Stokes parameters contrary to conventional methods, such as the M\"uller matrix one. The instrumental linear polarization is corrected across the whole telescope beam and significant Stokes $Q$ and $U$ can be recovered even when the recorded signals are severely corrupted. The accuracy we reach in terms of polarization degree is of the order of 0.1-0.2 %. The polarization angles are determined with an accuracy of almost 1$^{\circ}$. The presented methodology was applied to recover the linear and circular polarization of around 150 Active Galactic Nuclei. The sources were monitored from July 2010 to April 2016 with the Effelsberg 100-m telescope at 4.85 GHz and 8.35 GHz with a cadence of around 1.2 months. The polarized emission of the Moon was used to calibrate the polarization angle. Our analysis showed a small system-induced rotation of about 1$^{\circ}$ at both observing frequencies. Finally, we identify five sources with significant and stable linear polarization; three sources remain constantly linearly unpolarized over the period we examined; a total of 11 sources have stable circular polarization degree $m_\mathrm{c}$ and four of them with non-zero $m_\mathrm{c}$. We also identify eight sources that maintain a stable polarization angle over the examined period. All this is provided to the community for polarization observations reference. We finally show that our analysis method is conceptually different from the traditionally used ones and performs better than the M\"uller matrix method. Although it was developed for a system equipped with circularly polarized feeds it can easily be modified for systems with linearly polarized feeds as well.Comment: 19 pages, 17 figures, accepted for publication in Astronomy & Astrophysics on May 30, 201

Microwave Assisted Synthesis of Py-Im Polyamides

Microwave synthesis was utilized to rapidly build Py-Im polyamides in high yields and purity using Boc-protection chemistry on Kaiser oxime resin. A representative polyamide targeting the 5′-WGWWCW-3′ (W = A or T) subset of the consensus Androgen and Glucocorticoid Response Elements was synthesized in 56% yield after 20 linear steps and HPLC purification. It was confirmed by Mosher amide derivatization of the polyamide that a chiral α-amino acid does not racemize after several additional coupling steps