Behavioral observation used to estimate pesticide exposure for farm workers in Brazil

Assessment of the harm caused by exposure to pesticides requires that a measure of exposure be available. While such information is available without difficulty in controlled laboratory studies, estimating the exposure of humans who have been exposed in the real world is difficult. The difficulty is increased if exposures have taken place over an extended time period and the documentation of specifics is unavailable. Three methods of exposure assessment have previously been used: comparison of exposed and non-exposed groups, estimation of exposures through self-report of the individuals, and estimation of exposure through assessment of biomarkers or environmental levels. Each approach imposes limitations. We propose an additional approach - estimation of the degree of exposure for individuals through direct observation of their behavior and their use of personal protective equipment (PPE) during periods of exposure. We also obtain opinions from experts regarding the risk associated with the various behaviors and PPE use and combine these with the observations to create a personal risk index for each individual. By including information on chemicals in use during this period, we can characterize recent (observed) exposure for that individual. By these steps, the degree of risk may be determined for recent (observed) exposure. An estimate of long-term risk resulting from work-related exposures can be obtained for individuals by summing across their work histories

The dopamine D2/D3 receptor agonist quinpirole increases checking-like behaviour in an operant observing response task with uncertain reinforcement: a novel possible model of OCD.

Excessive checking is a common, debilitating symptom of obsessive-compulsive disorder (OCD). In an established rodent model of OCD checking behaviour, quinpirole (dopamine D2/3-receptor agonist) increased checking in open-field tests, indicating dopaminergic modulation of checking-like behaviours. We designed a novel operant paradigm for rats (observing response task (ORT)) to further examine cognitive processes underpinning checking behaviour and clarify how and why checking develops. We investigated i) how quinpirole increases checking, ii) dependence of these effects on D2/3 receptor function (following treatment with D2/3 receptor antagonist sulpiride) and iii) effects of reward uncertainty. In the ORT, rats pressed an 'observing' lever for information about the location of an 'active' lever that provided food reinforcement. High- and low-checkers (defined from baseline observing) received quinpirole (0.5mg/kg, 10 treatments) or vehicle. Parametric task manipulations assessed observing/checking under increasing task demands relating to reinforcement uncertainty (variable response requirement and active-lever location switching). Treatment with sulpiride further probed the pharmacological basis of long-term behavioural changes. Quinpirole selectively increased checking, both functional observing lever presses (OLPs) and non-functional extra OLPs (EOLPs). The increase in OLPs and EOLPs was long-lasting, without further quinpirole administration. Quinpirole did not affect the immediate ability to use information from checking. Vehicle and quinpirole-treated rats (VEH and QNP respectively) were selectively sensitive to different forms of uncertainty. Sulpiride reduced non-functional EOLPs in QNP rats but had no effect on functional OLPs. These data have implications for treatment of compulsive checking in OCD, particularly for serotonin-reuptake-inhibitor treatment-refractory cases, where supplementation with dopamine receptor antagonists may be beneficial

Shaping the location of a pigeon's peck: effect of rate and size of shaping steps.

For several pigeons, pecking at particular locations within a ten-inch-wide response area was reinforced by grain presentations. The reinforced locations changed systematically to "shape" response location back and forth across the area. The rate and size of these shifts in reinforced locations were varied in both between-subject and within-subject comparisons to evaluate the influence of these variables on the shaping process. Larger step sizes produced larger shifts in location for all sizes inspected, with all sizes from .5 to 3.0 inches effective in shaping behavior. More rapid steps were approximately as effective as slower steps for all rates of shift inspected from 25 reinforcers to 400 reinforcers per step. These data suggest that shaping peck location proceeds most efficiently with rapid, relatively large shifts in criterion performance

Latency and frequency of responding under discrete-trial fixed-interval schedules of reinforcement

Pigeons' key pecking was studied under a number of discrete-trial fixed-interval schedules of food reinforcement. Discrete trials were presented by briefly illuminating the keylight repetitively throughout the interreinforcement interval. A response latency counterpart to the fixed-interval scallop was found, latency showing a gradual, negatively accelerated decrease across the interval. This latency pattern was largely invariant across changes in fixed-interval length, number of trials per interval, and maximum trial duration. Frequency of responding during early trials in the intervals varied, however, with different schedule parameters, being directly related to fixed-interval length, inversely related to number of trials, and complexly affected by conjoint variations of fixed-interval length and number of trials. Response latency thus was found to be simply related to elapsed time during the interval while response frequency was complexly determined by other factors as well

Positive interaction (induction) in multiple variable-interval, differential-reinforcement-of-high-rate schedules

The effect of increases in the rate of responding in one component of a multiple schedule upon the rate of responding in a second component was investigated. Pigeons were exposed to a multiple schedule where both components were initially variable-interval schedules having the same parameter value. After rate of key pecking stabilized, one component was changed to a schedule that differentially reinforced high rates of responding. Rate of reinforcement in this varied component was adjusted to remain equal to rate of reinforcement in the constant (variable-interval) component. Four of five pigeons showed a maintained increase in rate of responding during both the constant and varied components, even though rates of reinforcement did not change