Vocalização de frangos de corte pode ser usada para identificar o sexo e a linhagem genética

In order to reach higher broiler performance, farmers target losses reduction. One way to make this possible is by rearing sexed broilers as male and female present diverse performance due to their physiological differences. Birds from different genetic strain also have a distinct performance at the same age. Considering that sexed flocks may present higher performance this study aimed to identify one-day-old chicks’ sex throughout their vocalization. This research also investigated the possibility of identifying the genetic strain by their vocalization attributes. A total of 120 chicks, half of them were from Cobb® genetic strain and the other half from Ross® genetic strain. From each group, a total of 30 were males and 30 females, which were previously separated by sex using their secondary physiological characteristics at the hatchery. Vocalizations audio recording was done inside a semi-anechoic chamber using a unidirectional microphone connected to an audio input of a digital recorder. Vocalizations were recorded for two minutes. Acoustic characteristics of the sounds were analyzed being calculated the fundamental frequency Pitch, the sound intensity, the first formant, and second formant. Results indicated that the vocalizations of both sexes could be identified by the second formant, and the genetic strain was detected by both the second formant and the Pitch.352192196FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESPSem informaçãoCom a finalidade de atingir maior desempenho em frangos de corte, produtores almejam redução de perdas. Uma das maneiras de se tornar isto possível é alojar lotes sexados, uma vez que machos e fêmeas apresentam desempenho distinto devido às diferenças fisiológicas. Aves de linhagens genéticas diferentes também apresentam desempenho distinto na mesma idade. Considerando que lotes sexados podem apresentar maior desempenho, este estudo objetivou identificar o sexo de pintos de um dia de idade através de sua vocalização. Esta pesquisa também investigou a possibilidade de identificar a linhagem genética através dos atributos da vocalização. O total de 120 pintos, sendo metade da genética Cobb® e a outra metade da genética Ross®, foram utilizados. De cada grupo, o total de 30 machos e 30 fêmeas foi previamente separado, utilizando suas características fisiológicas secundárias, no incubatório. O registro áudio de vocalização foi efetuado dentro de uma câmara semi anecoica, usando um microfone unidirecional conectado a uma entrada de áudio em um gravador digital. As vocalizações foram gravadas por dois minutos. As características acústicas dos sons foram analisadas e foram calculadas a frequência fundamental Pitch, a intensidade do som, a primeira formante e a segunda formante. Os resultados indicaram que a vocalização de ambos os sexos pode ser identificada pela segunda formante, e a linhagem genética pela segunda formante e pela frequência de Pitch

Socio-Economic and Governance Conditions Corresponding to Change in Animal Agriculture: South Dakota Case Study

Understanding sustainable livestock production requires consideration of both qualitative and quantitative factors in a temporal and/or spatial frame. This study adapted Qualitative Comparative Analysis (QCA) to relate conditions of social, economic, and governance factors to changes in livestock inventory across several counties and over time. This paper presents an approach that (1) identified factors with the potential to relate to a change in livestock inventory and (2) analyzed commonalities within these factors related to changes spatially and temporally. This paper illustrates the approach and results when applied to five counties in eastern South Dakota. The specific response variables were periods of increasing, no change, or decreasing beef cattle, dairy cattle, and swine inventories in the specific counties for five-year census periods between 1992 and 2017. In the spatial analysis of counties, stable beef inventories and decreasing dairy inventories related to counties with increasing gross domestic products. The presence of specific social communities related to increases in county swine inventories. In the temporal analysis of census periods, local governance and economic factors, particularly market price influences, were more prevalent. Swine inventory showed a stronger link to cash crop markets than to livestock markets, whereas cattle market price increases associated with stable inventories for all animal types. Local governance tools had mixed effects for the different animal types across space and time. The factors and analysis results are context-specific. However, the process considers the various socio-economic processes in livestock production and community development applicable to agricultural sustainability questions in the Midwest and beyond

Multiple Recombination Events Drive the Current Genetic Structure of Xanthomonas perforans in Florida

Prior to the identification of Xanthomonas perforans associated with bacterial spot of tomato in 1991, X. euvesicatoria was the only known species in Florida. Currently, X. perforans is the Xanthomonas sp. associated with tomato in Florida. Changes in pathogenic race and sequence alleles over time signify shifts in the dominant X. perforans genotype in Florida. We previously reported recombination of X. perforans strains with closely related Xanthomonas species as a potential driving factor for X. perforans evolution. However, the extent of recombination across the X. perforans genomes was unknown. We used a core genome multilocus sequence analysis approach to identify conserved genes and evaluated recombination-associated evolution of these genes in X. perforans. A total of 1,356 genes were determined to be “core” genes conserved among the 58 X. perforans genomes used in the study. Our approach identified three genetic groups of X. perforans in Florida based on the principal component analysis (PCA) using core genes. Nucleotide variation in 241 genes defined these groups, that are referred as Phylogenetic-group Defining (PgD) genes. Furthermore, alleles of many of these PgD genes showed 100% sequence identity with X. euvesicatoria, suggesting that variation likely has been introduced by recombination at multiple locations throughout the bacterial chromosome. Site-specific recombinase genes along with plasmid mobilization and phage associated genes were observed at different frequencies in the three phylogenetic groups and were associated with clusters of recombinant genes. Our analysis of core genes revealed the extent, source, and mechanisms of recombination events that shaped the current population and genomic structure of X. perforans in Florida

Gastos de energia na vocalização de suínos sob estresse

Modern swine production faces many challenges nowadays, among which are productivity growth, meat quality improvement, decrease of environmental damage and reduction of cost production. Pigs spend energy to vocalizing, especially when they undergo stress. The waste of energy can increase the cost of production and lead to greater environmental damage. The goal of this study was to estimate the energy spent by pigs under the stress of castration and its effect on the animals' weight gain. Two groups of ten animals each were castrated, being one group with local anesthetic and the other without anesthetic. The piglets' vocalizations were recorded during different stages of the neutering process and then estimated of the amount of energy emitted during each vocalization. Afterwards, this energy was associated with the animals' weight gain. There was no difference in the total energy spent in both groups, since the energy used during the application of anesthetic was similar to the energy spent during the castration of the animals without anesthetic. There was also no correlation between energy spent and the animals' weight gain. It was possible to estimate the amount of energy emitted through vocalization. This energy spent by piglets in pain was greater than in other handling situations (contention, weighting and realease).A suinocultura moderna enfrenta muitos desafios, dentre os quais o crescimento da produtividade, a melhoria da qualidade de carne, a redução de danos ambientais e a redução de custo de produção. Suínos gastam energia na vocalização, especialmente quando submetidos a determinado estresse. O desperdício de energia pode aumentar o custo de produção e conduzir a um maior dano ambiental. O objetivo deste estudo foi estimar a energia gasta pelos leitões sob o estresse da castração e seu efeito sobre o ganho de peso dos animais. Dois grupos de dez animais cada foram castrados, sendo um grupo com anestesia local e outro sem anestesia. As vocalizações dos leitões foram gravadas durante as diferentes fases do processo de castração, sendo objeto de uma estimativa da quantidade de energia emitida durante cada vocalização. Posteriormente, esta energia foi associada com o ganho de peso dos animais. Não houve diferença no gasto total da energia em ambos os grupos, uma vez que a energia utilizada durante a aplicação da anestesia foi semelhante à energia gasta durante a castração dos animais sem anestesia. Também não houve correlação entre a energia gasta e o ganho de peso dos animais. Foi possível estimar a quantidade de energia emitida através de vocalização. Esta energia gasta por leitões com dor foi maior do que em outras situações de manejo (contenção, pesagem e soltura).89690

Early Start Peritoneal Dialysis: Technique Survival in Long-Term Follow-Up

Background/Aims: Peritoneal dialysis (PD) has gained interest over the last decade as a viable option for early start dialysis. It is still unknown if shorter break-in periods and less time for proper patient evaluation and training could influence technique survival in comparison to planned-start PD. Methods: A prospective and observational study that compared technique survival in a cohort of patients who started either early or planned PD. Early start PD was defined as break-in period from 3 to 14 days with no previous nephrologist follow-up or patient training. Results: A total of 154 patients were included (40 as early start PD), followed by a median time of 381 days. Comparing early vs. planned-start PD, groups were similar concerning age 56 (40; 70) vs. 48 (32; 63) years, p=0.071, body mass index (BMI) 23.3 ± 4.2 vs. 23.8 ± 4.0 kg/m2, p=0.567 and male gender (60 vs. 48%, p=0.201), respectively. Comparing early vs. planned-start groups, there were no differences regarding PD dropout for peritonitis (7.5 vs. 11.4%, p=0.764), catheter dysfunction (12.5 vs. 17.5%, p=0.619) and patient burnout (0 vs. 4.4%, p=0.328), respectively. Less patients in early start group quit PD for peritoneal membrane failure in comparison to planned-start group (2.5 vs. 16.7%, p=0.026). In multivariate cox-regression analysis, the only factors independently associated with technique failure were BMI> 25 kg/m² (p=0.033) and Diabetes Mellitus (p=0.013), whereas no differences regarding early vs. planned-PD start were observed (p=0.184). Conclusion: Despite the adverse scenario for initiating dialysis, early start PD had similar outcomes in comparison to planned-start PD in long-term follow-up

Water aerobics II: maternal body composition and perinatal outcomes after a program for low risk pregnant women

<p>Abstract</p> <p>Background</p> <p>To evaluate the effectiveness and safety of water aerobics during pregnancy.</p> <p>Methods</p> <p>A randomized controlled trial carried out in 71 low-risk sedentary pregnant women, randomly allocated to water aerobics or no physical exercise. Maternal body composition and perinatal outcomes were evaluated. For statistical analysis Chi-square, Fisher's or Student's t-tests were applied. Risk ratios and their 95% CI were estimated for main outcomes. Body composition was evaluated across time using MANOVA or Friedman multiple analysis.</p> <p>Results</p> <p>There were no significant differences between the groups regarding maternal weight gain, BMI or percentage of body fat during pregnancy. Incidence of preterm births (RR = 0.84; 95%CI:0.28–2.53), vaginal births (RR = 1.24; 95%CI:0.73–2.09), low birthweight (RR = 1.30; 95%CI:0.61–2.79) and adequate weight for gestational age (RR = 1.50; 95%CI:0.65–3.48) were also not significantly different between groups. There were no significant differences in systolic and diastolic blood pressure and heart rate between before and immediately after the water aerobics session.</p> <p>Conclusion</p> <p>Water aerobics for sedentary pregnant women proved to be safe and was not associated with any alteration in maternal body composition, type of delivery, preterm birth rate, neonatal well-being or weight.</p

The EuRRECa project as a model for data access and governance policies for rare disease registries that collect clinical outcomes

No abstract available

A consensus statement for trauma surgery capacity building in Latin America

Background Trauma is a significant public health problem in Latin America (LA), contributing to substantial death and disability in the region. Several LA countries have implemented trauma registries and injury surveillance systems. However, the region lacks an integrated trauma system. The consensus conference’s goal was to integrate existing LA trauma data collection efforts into a regional trauma program and encourage the use of the data to inform health policy. Methods We created a consensus group of 25 experts in trauma and emergency care with previous data collection and injury surveillance experience in the LA. region. Experts participated in a consensus conference to discuss the state of trauma data collection in LA. We utilized the Delphi method to build consensus around strategic steps for trauma data management in the region. Consensus was defined as the agreement of ≥ 70% among the expert panel. Results The consensus conference determined that action was necessary from academic bodies, scientific societies, and ministries of health to encourage a culture of collection and use of health data in trauma. The panel developed a set of recommendations for these groups to encourage the development and use of robust trauma information systems in LA. Consensus was achieved in one Delphi round. Conclusions The expert group successfully reached a consensus on recommendations to key stakeholders in trauma information systems in LA. These recommendations may be used to encourage capacity building in trauma research and trauma health policy in the region