Circulating microRNA as Biomarkers for Gestational Diabetes Mellitus—A Systematic Review and Meta-Analysis

Gestational diabetes mellitus (GDM) is a severe pregnancy complication for both the woman and the child. Women who suffer from GDM have a greater risk of developing Type 2 diabetes mellitus (T2DM) later in life. Identification of any potential biomarkers for the early prediction of gestational diabetes can help prevent the disease in women with a high risk. Studies show microRNA (miRNA) as a potential biomarker for the early discovery of GDM, but there is a lack of clarity as to which miRNAs are consistently altered in GDM. This study aimed to perform a systematic review and meta-analysis to investigate miRNAs associated with GDM by comparing GDM cases with normoglycemic controls. The systematic review was performed according to PRISMA guidelines with searches in PubMed, Web of Science, and ScienceDirect. The primary search resulted in a total of 849 articles, which were screened according to the prior established inclusion and exclusion criteria. Following the screening of articles, the review was based on the inclusion of 35 full-text articles, which were evaluated for risk of bias and estimates of quality, after which data were extracted and relative values for miRNAs were calculated. A meta-analysis was performed for the miRNA species investigated in three or more studies: MiR-29a, miR-330, miR-134, miR-132, miR-16, miR-223, miR-155, miR-122, miR-17, miR-103, miR-125, miR-210, and miR-222. While some miRNAs showed considerable between-study variability, miR-29a, miR-330, miR-134, miR-16, miR-223, and miR-17 showed significant overall upregulation in GDM, while circulating levels of miR-132 and miR-155 were decreased among GDM patients, suggesting further studies of these as biomarkers for early GDM discovery.</p

Cosmological Simulations of Massive Compact High-z Galaxies

In order to investigate the structure and dynamics of the recently discovered massive (M_* > 10^11 M_sun) compact z~2 galaxies, cosmological hydrodynamical/N-body simulations of a proto-cluster region have been undertaken. At z=2, the highest resolution simulation contains ~5800 resolved galaxies, of which 509, 27 and 5 have M_* > 10^10 M_sun, > 10^11 M_sun and > 4x10^11 M_sun, respectively. Effective radii and characteristic stellar densities have been determined for all galaxies. At z=2, for the definitely well resolved mass range of M_* > 10^11 Msun, the mass-size relation is consistent with observational findings for the most compact z~2 galaxies. The very high velocity dispersion recently measured for a compact z~2 galaxy (~510 km/s; van Dokkum et al 2009) can be matched at about the 1-sigma level, although a somewhat larger mass than the estimated M_* ~ 2 x 10^11 M_sun is indicated. For the above mass range, the galaxies have an average axial ratio = 0.64 +/- 0.02 with a dispersion of 0.1, an average rotation to 1D velocity dispersion ratio = 0.46 +/- 0.06 with a dispersion of 0.3, and a maximum value of v/sigma ~ 1.1. Rotation and velocity anisotropy both contribute in flattening the compact galaxies. Some of the observed compact galaxies appear flatter than any of the simulated galaxies. Finally, it is found that the massive compact galaxies are strongly baryon dominated in their inner parts, with typical dark matter mass fractions of order only 20% inside of r=2R_eff.Comment: 10 pages, 8 figures, submitted to Ap

Bose-Einstein Condensates in Optical Lattices: Band-Gap Structure and Solitons

We analyze the existence and stability of spatially extended (Bloch-type) and localized states of a Bose-Einstein condensate loaded into an optical lattice. In the framework of the Gross-Pitaevskii equation with a periodic potential, we study the band-gap structure of the matter-wave spectrum in both the linear and nonlinear regimes. We demonstrate the existence of families of spatially localized matter-wave gap solitons, and analyze their stability in different band gaps, for both repulsive and attractive atomic interactions

The extent of error-prone replication-restart by homologous recombination is controlled by Exo1 and checkpoint proteins

Genetic instability, a hallmark of cancer, can occur when the replication machinery encounters a barrier. The intra-S phase checkpoint maintains stalled replication forks in a replication-competent configuration by phosphorylating replisome components and DNA repair proteins to prevent forks from catastrophically collapsing. Here we report a novel Chk1- and Cds1Chk2-independent function for Rad3ATR, the core S. pombe checkpoint sensor kinase: Rad3ATR regulates the association of recombination factors with collapsed forks thus limiting their genetic instability. We further reveal antagonistic roles for Rad3ATR and the 9-1-1 clamp: Rad3ATR restrains MRN- and Exo1-dependent resection while the 9-1-1 complex promotes Exo1 activity. Interestingly the MRN complex, but not its nuclease activity, promotes resection and the subsequent association of recombination factors at collapsed forks. The biological significance of this regulation is revealed by the observation that Rad3ATR prevents Exo1-dependent genome instability upstream a collapsed fork without affecting the efficiency of recombination-mediated replication-restart. We propose the interplay between Rad3ATR and the 9-1-1 clamp functions to fine-tune the balance between the need for recovery of replication via recombination and the risk of increased genome instability