Inhibition, recovery and field responses of Astyanax fasciatus (Cuvier, 1819) brain cholinesterases upon exposure to azinphos-methyl

Pesticides used in agriculture are among the most important environmental pollutants. Organophosphate and carbamate insecticides, intensely used in deciduous fruit-trees, may be  transported to aquatic ecosystems by runoff. Northwest rural Montevideo possesses zones of fruit-tree farms, where azinphos-methyl is currently the most used pesticide. Despite the well-known neurotoxic properties of this agrochemical, studies of its effects on aquatic organisms are scarce in Uruguay. The main goal of this study was to evaluate effects on brain cholinesterases and erythrocyte micronuclei in Astyanax fasciatus exposed to azynphos-methyl in laboratory and field conditions. Dose-response curves showed concentration-dependent brain cholinesterase inhibitions and a LC50 (48-hour) of 2.31 mg L-1 for azinphos-methyl.  Fishes exposed for 48 hours to toxicant and then transferred to clear water recovered 80% of brain cholinesterase activity in 10 days. Field study indicated that A. fasciatus from a watershed with low contamination showed a brain cholinesterase specific activity of 62.2±5.1 Units.mg-1 protein (22ºC) 97.7 % of which was acetylcholinesterase. Specimens from a basin with intense fruticulture exhibited a spatial gradient: specimens collected downstream to the farms showed brain acetylcholinesterase activities 32% lower than fishes captured upstream. In conclusion, our data suggest that A. fasciatus is a suitable species for exotoxicological biomonitoring.Keywords: Acetylcholinesterase; Astyanax fasciatus; Aquatic Biomonitoring; Azinphos-methyl; Bioindicator; Brain cholinesterases; Fish; Organophosphate pesticide

Circulatory immune cells in Cushing syndrome: bystanders or active contributors to atherometabolic injury? A study of adhesion and activation of cell surface markers.

Glucocorticoids (GC) induce cardiometabolic risk while atherosclerosis is a chronic inflammation involving immunity. GC are immune suppressors, and the adrenocorticotrophic hormone (ACTH) has immune modulator activities. Both may act in atherothrombotic inflammation involving immune cells (IMNC). Aim. To investigate adhesion and activation surface cell markers (CDs) of peripheral IMNC in endogenous Cushing syndrome (CS) and the immune modulator role of ACTH. Material and Methods. 16 ACTH-dependent CS (ACTH-D), 10 ACTH-independent (ACTH-ID) CS, and 16 healthy controls (C) were included. Leukocytes (Leuc), monocytes (MN), lymphocytes (Lym), and neutrophils (N) were analyzed by flow cytometry for atherosclerosis previously associated with CDs. Results. Leuc, N, and MN correlated with CS (p < 0.05), WC (p < 0.001), WHR (p = 0.003), BMI (p < 0.001), and hs-CRP (p < 0.001). CD14++CD16+ (p = 0.047); CD14+CD16++ (p = 0.053) MN; CD15+ (p = 0.027); CD15+CD16+ (p = 0.008) N; and NK-Lym (p = 0.019) were higher in CS. CD14+CD16++ MN were higher in ACTH-ID (8.9 ± 3.5%) versus ACTH-D CS (4.2 ± 1.9%) versus C (4.9 ± 2.3%). NK-Lym correlated with c-LDL (r = 0.433, p = 0.039) and CD15+ N with hs-CRP (r = 0.446, p = 0.037). In multivariate analysis, Leuc, N, and MN depended on BMI (p = 0.021), WC (p = 0.002), and WHR (p = 0.014), while CD15+ and CD15+CD16+ N on hypercortisolism and CS (p = 0.035). Conclusion. In CS, IMNC present changes in activation and adhesion CDs implicated in atherothrombotic inflammation. ACTH-IDCS presents a particular IMNC phenotype, possibly due to the absence of the immune modulator effect of ACTH

A Model of Continuous Improvement Programme Management

The aim of this study is to identify key management decisions that enable the sustainment of a continuous improvement (CI) initiative. To accomplish this aim, we examine the procedures and practices used by two manufacturing companies for the management of their CI initiatives; one that is successfully sustaining the effectiveness of its CI initiative and another failing to do the same. This research makes two contributions to the conceptual understanding of CI programme management. First, we identify five CI programme management factors that enable the sustainment of a CI initiative. Second, the five factors are incorporated into a new CI programme management model. The model details a ‘bottom-up’ procedure for the generation of manufacturing performance improvement ideas and the management of their implementation

Association of a single nucleotide polymorphism combination pattern of the Klotho gene with non-cardiovascular death in patients with chronic kidney disease

Chronic kidney disease (CKD) is associated with an elevated risk of all-cause mortality, with cardiovascular death being extensively investigated. However, non-cardiovascular mortality represents the biggest percentage, showing an evident increase in recent years. Klotho is a gene highly expressed in the kidney, with a clear influence on lifespan. Low levels of Klotho have been linked to CKD progression and adverse outcomes. Single nucleotide polymorphisms (SNPs) of the Klotho gene have been associated with several diseases, but studies investigating the association of Klotho SNPs with noncardiovascular death in CKD populations are lacking. The main aim of this study was to assess whether 11 Klotho SNPs were associated with non-cardiovascular death in a subpopulation of the National Observatory of Atherosclerosis in Nephrology (NEFRONA) study (n ¼ 2185 CKD patients). After 48 months of follow-up, 62 cardiovascular deaths and 108 non-cardiovascular deaths were recorded. We identified a high non-cardiovascular death risk combination of SNPs corresponding to individuals carrying the most frequent allele (G) at rs562020, the rare allele (C) at rs2283368 and homozygotes for the rare allele (G) at rs2320762 (rs562020 GG/AG þ rs2283368 CC/CT þ rs2320762 GG). Among the patients with the three SNPs genotyped (n ¼ 1016), 75 (7.4%) showed this combination. Furthermore, 95 (9.3%) patients showed a low-risk combination carrying all the opposite genotypes (rs562020 AA þ rs2283368 TT þ rs2320762 GT/TT). All the other combinations [n ¼ 846 (83.3%)] were considered as normal risk. Using competing risk regression analysis, we confirmed that the proposed combinations are independently associated with a higher fhazard ratio [HR] 3.28 [confidence interval (CI) 1.51-7.12]g and lower [HR 6 × 10- (95% CI 3.3 × 10--1.1 × 10-)] risk of suffering a non-cardiovascular death in the CKD population of the NEFRONA cohort compared with patients with the normal-risk combination. Determination of three SNPs of the Klotho gene could help in the prediction of non-cardiovascular death in CKD

Automation Process of 3D Scan Based Brace Design

Approximately 83,700 children across the United States and 1.7 million children worldwide are affected by cerebral palsy and have limited or no walking ability. [1] Customizable exoskeletons could greatly improve rehabilitation outcomes and quality of life for children suffering from diseases such as cerebral palsy and spina bifida which limit mobility. Currently, it takes an expert 5-6 hours to create a single set of exoskeleton braces from one 3D scan imported into SolidWorks. In this project, we developed a process that enables braces created from a template 3D scan to readjust to another with minimal steps. This is critical as the braces must be modified as children grow. The process of solid modelling typically involves orienting objects, such as the braces, in reference to 3D space. Instead, by defining the braces using global variables and joint references like the hip, knee, and ankle, braces can transfer from the template scan to another scan with automatic size and orientation adjustment. The methodology of brace transfer involves changing the reference surface and joint of the template brace from one scan to another and can be done within minutes. This technique standardizes the design and drastically shortens the time it takes to create custom-fitted braces while reducing human error. Future work would expand the proof of concept to create a complete set of braces that transfers between scans. Applications for this research are not limited to exoskeletons but can be applied to any customizable 3D scan based orthotic/prosthetic. [1]https://www.cdc.gov/ncbddd/cp/data.htmlBiomedical Engineering, Department ofHonors Colleg

Planificación de la actividad rural ganadera en la comarca de Laciana. Un proyecto de investigación y desarrollo rural

En el presente artículo se analizan los resultados de un proyecto de investigación planeado con el [m de realizar una planificación de la actividad rural ganadera en la Comarca de Laciana. Constituye una zona desfavorecida de montaña, con una problemática económica, social y ambiental, derivada de la crisis de la minería que constituyó su soporte económico. En una primera fase se establece el enmarque conceptual del desanollo rural de la zona y, posteriormente, se presentan los datos obtenidos mediante una encuesta realizada con el objetivo de determinar la situación actual del sector ganadero, tanto desde la perspectiva de la valoración económica de las explotaciones, como las opiniones de los ganaderos sobre su situación y expectativas. Por último se exponen las actividades realizadas en orden a la puesta en marcha un Plan de desarrollo endógeno de la zona