Plant Growth Promoting of Endophytic Sporosarcina aquimarina

Endophytic Sporosarcina aquimarina SjAM16103 was isolated from the inner tissues of pneumatophores of mangrove plant Avicennia marina along with Bacillus sp. and Enterobacter sp. Endophytic S. aquimarina SjAM16103 was Gram variable, and motile bacterium measured 0.6–0.9 μm wide by 1.7–2.0 μm long and light orange-brown coloured in 3-day cultures on tryptone broth at 26°C. Nucleotide sequence of this strain has been deposited in the GenBank under accession number GU930359. This endophytic bacterium produced 2.37 μMol/mL of indole acetic acid and siderophore as it metabolites. This strain could solubilize phosphate molecules and fixes atmospheric nitrogen. Endophytic S. aquimarina SjAM16103 was inoculated into four different plants under in vitro method to analyse its growth-promoting activity and role inside the host plants. The growth of endophytic S. aquimarina SjAM16103 inoculated explants were highly significant than the uninoculated control explants. Root hairs and early root development were observed in the endophytic S. aquimarina SjAM16103 inoculated explants

Micropropagation of <i style="">Sauropus androgynus </i>(L.) Merr.—An important green leafy vegetable

235-237A micropropagation protocol has been developed for Sauropus androgynus (L.) Merr. using uninodal explants in Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinins, benzyl adenine (BA) and kinetin (Kn). Shoot induction was observed in 1.0 mg/L BA and 0.1 mg/L Kn after 25 d and more number of shoots was achieved in shoot induction medium. Rooting was induced from shoots in MS medium supplemented with various concentrations of indole 3-butyric acid (IBA) and naphthalene acetic acid (NAA). All the shoots were rooted in 0.5 mg/L IBA and 0.2 mg/L NAA after 25 d. Rooted plants were transferred to soil and successfully acclimatized