Uma proposta de classificação de analogias para o ensino nas ciências da saúde

O objetivo desse trabalho é contribuir para o processo de ensino de Ciências da Saúde por meio da apresentação de uma nova proposta de classificação de analogias contendo suas categorias, ca­racterizações e tipologias, elaborada a partir de duas dissertações de mestrado. Sua formulação ocorreu após análise de livros didáticos de Enfermagem e de Fisioterapia, de questionários e entrevistas com profissionais das áreas. A proposta contém oito categorias com respectivas caracterizações e apresenta uma tipologia própria, flexível de modo a ser reconstruída e se aplica a outras áreas do conhecimento. Os resultados apontam a necessidade de ampliar o sistema de classificação utilizado no ensino de ciên­cias de modo a contemplar as analogias encontradas no presente estudo

Distributed Energy Resources Parameter Monitoring in Microgrids Using Blockchain and Edge Computing

An increased share of distributed renewable energy sources requires flexible tools for providing reliable and cheap electricity. Smart meters provide information at the consumer level, which could be used as the main source for real-time energy micro-transactions, however, one of the main concerns about direct transactions is information security. Conventional electricity markets rely on centralized information exchange, nevertheless, when intra-day, distributed, electricity consumption and production exchanges are required between customers, this approach might not be enough. This paper presents a proof-of-concept for using Blockchain as a tool for managing the operational transactions in a DC microgrid. The distributed nature of this technology provides an inherently safer approach, by providing an immutable database for transaction history. One of the challenges of using this technology, however, is the required computing power at the nodes and the limited capacity available in the smart meter. To overcome these issues, the authors used a distributed computing technology,-edge computing-, where computation and storage are located closer to the customer, to improve response times by handling the required computational tasks of the Blockchain tool. This approach proved not only to be practically viable but also, offers important insights about the scalability and capabilities of the technology. © 2020 IEEE

Geometric evaluation of stiffened steel plates subjected to transverse loading for naval and offshore applications

This work searched for the optimal geometrical configuration of simply supported stiffened plates subjected to a transverse and uniformly distributed load. From a non-stiffened reference plate, different geometrical configurations of stiffened plates, with the same volume as the reference plate, were defined through the constructal design method. Thus, applying the exhaustive search technique and using the ANSYS software, the mechanical behaviors of all the suggested stiffened plates were compared to each other to find the geometrical configuration that provided the minimum deflection in the plate's center when subjected to this loading. The optimum geometrical configuration of stiffeners is presented at the end of this work, allowing a reduction of 98.57% for the central deflection of the stiffened plate if compared to the reference plate. Furthermore, power equations were adjusted to describe the deflections for each combination of longitudinal and transverse stiffeners as a function of the ratio between the height and the thickness of the stiffeners. Finally, a unique equation for determining the central deflections of the studied stiffened plates based only on the number of longitudinal stiffeners without significantly losing accuracy has been proposed

First normal stress difference and crystallization in a dense sheared granular fluid

The first normal stress difference (${\mathcal N}_1$) and the microstructure in a dense sheared granular fluid of smooth inelastic hard-disks are probed using event-driven simulations. While the anisotropy in the second moment of fluctuation velocity, which is a Burnett-order effect, is known to be the progenitor of normal stress differences in {\it dilute} granular fluids, we show here that the collisional anisotropies are responsible for the normal stress behaviour in the {\it dense} limit. As in the elastic hard-sphere fluids, ${\mathcal N}_1$ remains {\it positive} (if the stress is defined in the {\it compressive} sense) for dilute and moderately dense flows, but becomes {\it negative} above a critical density, depending on the restitution coefficient. This sign-reversal of ${\mathcal N}_1$ occurs due to the {\it microstructural} reorganization of the particles, which can be correlated with a preferred value of the {\it average} collision angle $\theta_{av}=\pi/4 \pm \pi/2$ in the direction opposing the shear. We also report on the shear-induced {\it crystal}-formation, signalling the onset of fluid-solid coexistence in dense granular fluids. Different approaches to take into account the normal stress differences are discussed in the framework of the relaxation-type rheological models.Comment: 21 pages, 13 figure

Comparative Survival Analysis of Deinococcus Radiodurans and the Haloarchaea Natrialba Magadii and Haloferax Volcanii, Exposed to Vacuum Ultraviolet Irradiation

The haloarchaea Natrialba magadii and Haloferax volcanii, as well as the radiation-resistant bacterium Deinococcus radiodurans, were exposed to vacuum-UV (V-UV) radiation at the Brazilian Synchrotron Light Laboratory (LNLS). Cell monolayers (containing 105 - 106 cells per sample) were prepared over polycarbonate filters and irradiated under high vacuum (10-5 Pa) with polychromatic synchrotron radiation. N. magadii was remarkably resistant to high vacuum with a survival fraction of ((3.77 \pm 0.76) x 10-2), larger than the one of D. radiodurans ((1.13 \pm 0.23) x 10-2). The survival fraction of the haloarchaea H. volcanii, of ((3.60 \pm 1.80) x 10-4), was much smaller. Radiation resistance profiles were similar between the haloarchaea and D. radiodurans for fluencies up to 150 J m-2. For fluencies larger than 150 J m-2 there was a significant decrease in the survival of haloarchaea, and in particular H. volcanii did not survive. Survival for D. radiodurans was 1% after exposure to the higher V-UV fluency (1350 J m-2) while N. magadii had a survival lower than 0.1%. Such survival fractions are discussed regarding the possibility of interplanetary transfer of viable micro-organisms and the possible existence of microbial life in extraterrestrial salty environments such as the planet Mars and the Jupiter's moon Europa. This is the first work reporting survival of haloarchaea under simulated interplanetary conditions.Comment: Draft version (without figures), Accepted for publication in Astrobiolog

Prevalence of Clostridioides difficile in dogs (Canis familiaris) with gastrointestinal disorders in Rio de Janeiro

Clostridioides difficile infections (CDI) have a high morbidity and mortality rate and have always been considered a nosocomial disease. Nonetheless, the number of cases of community-acquired CDI is increasing, and new evidence suggests additional C. difficile reservoirs exist. Pathogenic C. difficile strains have been found in livestock, domestic animals, and meat, so a zoonotic transmission has been proposed. Objective: The goal of this study was to isolate C. difficile strains in dogs at a veterinary clinic in Rio de Janeiro, Brazil, and characterize clinical and pathological findings associated with lower gastrointestinal tract disorders. Methods: Fifty stool samples and biopsy fragments from dogs were obtained and cultured in the CDBA selective medium. All suggestive C. difficile colonies were confirmed by MALDI-TOF MS and PCR (tpi gene). Vancomycin, metronidazole, moxifloxacin, erythromycin, and rifampicin were tested for antibiotic susceptibility. Biofilm, motility assays, and a PCR for the toxins (tcdA, tcdB, and cdtB), as well as ribotyping, were also performed. Results: Blood samples and colonic biopsy fragments were examined in C. difficile positive dogs. Ten animals (20%) tested positive for C. difficile by using stool samples, but not from biopsy fragments. Most C. difficile strains were toxigenic: six were A+B+ belonging to RT106; two were A+B+ belonging to RT014/020; and two were AB- belonging to RT010. All strains were biofilm producers. In the motility test, 40% of strains were as motile as the positive control, CD630 (RT012). In the disc diffusion test, two strains (RT010) were resistant to erythromycin and metronidazole; and another to metronidazole (RT014/020). In terms of C. difficile clinicopathological correlations, no statistically significant morphological changes, such as pseudomembranous and "volcano" lesions, were observed. Regarding hematological data, dogs positive for C. difficile had leucopenia (p = 0.02) and lymphopenia (p = 0.03). There was a significant correlation between senility and the presence of C. difficile in the dogs studied (p = 0,02). Conclusions: Although C. difficile has not been linked to canine diarrheal disorders, it appears to be more common in dogs with intestinal dysfunctions. The isolation of ribotypes frequently involved in human CDI outbreaks around the world supports the theory of C. difficile zoonotic transmission.Molecular basis of bacterial pathogenesis, virulence factors and antibiotic resistanc

Kinin b(1) receptor in adipocytes regulates glucose tolerance and predisposition to obesity

BACKGROUND: Kinins participate in the pathophysiology of obesity and type 2 diabetes by mechanisms which are not fully understood. Kinin B(1) receptor knockout mice (B(1) (-/-)) are leaner and exhibit improved insulin sensitivity. METHODOLOGY/PRINCIPAL FINDINGS: Here we show that kinin B(1) receptors in adipocytes play a role in controlling whole body insulin action and glucose homeostasis. Adipocytes isolated from mouse white adipose tissue (WAT) constitutively express kinin B(1) receptors. In these cells, treatment with the B(1) receptor agonist des-Arg(9)-bradykinin improved insulin signaling, GLUT4 translocation, and glucose uptake. Adipocytes from B(1) (-/-) mice showed reduced GLUT4 expression and impaired glucose uptake at both basal and insulin-stimulated states. To investigate the consequences of these phenomena to whole body metabolism, we generated mice where the expression of the kinin B(1) receptor was limited to cells of the adipose tissue (aP2-B(1)/B(1) (-/-)). Similarly to B(1) (-/-) mice, aP2-B(1)/B(1) (-/-) mice were leaner than wild type controls. However, exclusive expression of the kinin B(1) receptor in adipose tissue completely rescued the improved systemic insulin sensitivity phenotype of B(1) (-/-) mice. Adipose tissue gene expression analysis also revealed that genes involved in insulin signaling were significantly affected by the presence of the kinin B(1) receptor in adipose tissue. In agreement, GLUT4 expression and glucose uptake were increased in fat tissue of aP2-B(1)/B(1) (-/-) when compared to B(1) (-/-) mice. When subjected to high fat diet, aP2-B(1)/B(1) (-/-) mice gained more weight than B(1) (-/-) littermates, becoming as obese as the wild types. CONCLUSIONS/SIGNIFICANCE: Thus, kinin B(1) receptor participates in the modulation of insulin action in adipocytes, contributing to systemic insulin sensitivity and predisposition to obesity

APICULTURA SUSTENTÁVEL: PRODUÇÃO E COMERCIALIZAÇÃO DE MEL NO MEIO OESTE CATARINENSE

O presente trabalho tem como objetivo fomentar a produção de mel em larga escala, utilizando-se da apicultura de forma sustentável. A organização dos atores locais em Arranjos Produtivos será o principal mecanismo estratégico para gerar capital social e fomentar o projeto. A produção e a comercialização de mel no meio oeste catarinense será realizado através da criação de uma associação entre os moradores residentes no interior dos municípios integrantes da AMMOC (Associação dos Municípios do Meio Oeste Catarinense), e em pareceria com entidades necessárias na execução, como o Serviço Brasileiro de Apoio às Micro e Pequenas Empresas (SEBRAE), Associações de Apicultores e Meliponicultores de Santa Catarina (FAASC), Associação Brasileira dos Exportadores de Mel (ABEMEL), Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (EPAGRI). A apicultura é uma atividade econômica de baixo impacto ambiental, que possibilita a utilização dos recursos naturais, de maneira que contribui com a manutenção e a preservação dos ecossistemas já existentes. O fato é que as abelhas atuam como polinizadores naturais tanto das espécies nativas como também das cultivadas, promovendo a preservação da biodiversidade e contribuindo com o equilíbrio do ecossistema.  Em sintese o projeto tem como meta a expansão no setor apiário na região da AMOC, e conclui-se ser uma atividade economicamente rentável e ecologicamente prudente, a fim de contribuir para o desenvolvimento, gerando emprego e renda para a regiã

The Brazilian Developments On The Regional Atmospheric Modeling System (brams 5.2): An Integrated Environmental Model Tuned For Tropical Areas

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)We present a new version of the Brazilian developments on the Regional Atmospheric Modeling System (BRAMS), in which different previous versions for weather, chemistry, and carbon cycle were unified in a single integrated modeling system software. This new version also has a new set of state-of-the-art physical parameterizations and greater computational parallel and memory usage efficiency. The description of the main model features includes several examples illustrating the quality of the transport scheme for scalars, radiative fluxes on surface, and model simulation of rainfall systems over South America at different spatial resolutions using a scale aware convective parameterization. Additionally, the simulation of the diurnal cycle of the convection and carbon dioxide concentration over the Amazon Basin, as well as carbon dioxide fluxes from biogenic processes over a large portion of South America, are shown. Atmospheric chemistry examples show the model performance in simulating near-surface carbon monoxide and ozone in the Amazon Basin and the megacity of Rio de Janeiro. For tracer transport and dispersion, the model capabilities to simulate the volcanic ash 3-D redistribution associated with the eruption of a Chilean volcano are demonstrated. The gain of computational efficiency is described in some detail. BRAMS has been applied for research and operational forecasting mainly in South America. Model results from the operational weather forecast of BRAMS on 5km grid spacing in the Center for Weather Forecasting and Climate Studies, INPE/Brazil, since 2013 are used to quantify the model skill of near-surface variables and rainfall. The scores show the reliability of BRAMS for the tropical and subtropical areas of South America. Requirements for keeping this modeling system competitive regarding both its functionalities and skills are discussed. Finally, we highlight the relevant contribution of this work to building a South American community of model developers. © Author(s) 2017.1011892222014/01563-1, FAPESP, Fundação de Amparo à Pesquisa do Estado de São Paulo2014/01564-8, FAPESP, Fundação de Amparo à Pesquisa do Estado de São Paulo2015/10206-0, FAPESP, Fundação de Amparo à Pesquisa do Estado de São Paulo306340/2011-9, Conselho Nacional de Desenvolvimento Científico e TecnológicoFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq