\u3cem\u3eRhizobium japonicum\u3c/em\u3e Mutants Defective in Symbiotic Nitrogen Fixation

Rhizobium japonicum strains 3I1b110 and 61A76 were mutagenized to obtain 25 independently derived mutants that produced soybean nodules defective in nitrogen fixation, as assayed by acetylene reduction. The proteins of both the bacterial and the plant portions of the nodules were analyzed by two-dimensional polyacrylamide gel electrophoresis. All of the mutants had lower-than-normal levels of the nitrogenase components, and all but four contained a prominent bacteroid protein not observed in wild-type bacteroids. Experiments with bacteria grown ex planta suggested that this protein was derepressed by the absence of ammonia. Nitrogenase component II of one mutant was altered in isoelectric point. The soluble plant fraction of the nodules of seven mutants had very low levels of heme, yet the nodules of five of these seven mutants contained the polypeptide of leghemoglobin. Thus, the synthesis of the globin may not be coupled to the content of available heme in soybean nodules. The nodules of the other two of these seven mutants lacked not only leghemoglobin but most of the other normal plant and bacteroid proteins. Ultrastructural examination of nodules formed by these two mutants indicated normal ramification of infection threads but suggested a problem in subsequent survival of the bacteria and their release from the infection threads

Spitzer observations of Bow Shocks and Outflows in RCW 38

We report Spitzer observations of five newly identified bow shocks in the massive star-forming region RCW 38. Four are visible at IRAC wavelengths, the fifth is visible only at 24 microns. Chandra X-ray emission indicates that winds from the central O5.5 binary, IRS~2, have caused an outflow to the NE and SW of the central subcluster. The southern lobe of hot ionised gas is detected in X-rays; shocked gas and heated dust from the shock-front are detected with Spitzer at 4.5 and 24 microns. The northern outflow may have initiated the present generation of star formation, based on the filamentary distribution of the protostars in the central subcluster. Further, the bow-shock driving star, YSO 129, is photo-evaporating a pillar of gas and dust. No point sources are identified within this pillar at near- to mid-IR wavelengths. We also report on IRAC 3.6 & 5.8 micron observations of the cluster DBS2003-124, NE of RCW 38, where 33 candidate YSOs are identified. One star associated with the cluster drives a parsec-scale jet. Two candidate HH objects associated with the jet are visible at IRAC and MIPS wavelengths. The jet extends over a distance of ~3 pc. Assuming a velocity of 100 km/s for the jet material gives an age of about 30,000 years, indicating that the star (and cluster) are likely to be very young, with a similar or possibly younger age than RCW 38, and that star formation is ongoing in the extended RCW 38 region.Comment: 27 pages, 6 figures, accepted to Ap

Ant colony optimisation and local search for bin-packing and cutting stock problems

The Bin Packing Problem and the Cutting Stock Problem are two related classes of NP-hard combinatorial optimization problems. Exact solution methods can only be used for very small instances, so for real-world problems, we have to rely on heuristic methods. In recent years, researchers have started to apply evolutionary approaches to these problems, including Genetic Algorithms and Evolutionary Programming. In the work presented here, we used an ant colony optimization (ACO) approach to solve both Bin Packing and Cutting Stock Problems. We present a pure ACO approach, as well as an ACO approach augmented with a simple but very effective local search algorithm. It is shown that the pure ACO approach can compete with existing evolutionary methods, whereas the hybrid approach can outperform the best-known hybrid evolutionary solution methods for certain problem classes. The hybrid ACO approach is also shown to require different parameter values from the pure ACO approach and to give a more robust performance across different problems with a single set of parameter values. The local search algorithm is also run with random restarts and shown to perform significantly worse than when combined with ACO

Inclusive Masculinity and Facebook Photographs Among Early Emerging Adults at a British University

Central to debates about the construction of masculinity in sociology is the influence of culture and what constitutes acceptable displays of masculinity. This article adopts a novel approach in examining this question. It adopts a summative content analysis, combined with a semiotic analysis, of 1,100 Facebook photographs, in order to explore the underlying meanings within the photos and the performances of masculinity. Facebook photographs from 44, straight, White, male, early emerging adults attending the same university are used as a representation of an individual’s ideal self. These are then analyzed in order to determine the behaviors endorsed by peer culture. It was found that the sample overwhelmingly adopted inclusive behaviors (including homosocial tactility, dancing, and kissing each other), and inclusive masculinity theory was utilized to contextualize participants’ constructions of masculinity. Thus, this research shows that emerging adult males at this university construct their masculine identities away from previous orthodox archetypes. It is argued that the reducing importance of gendered behavior patterns may represent an adoption of what are perceived as wider cultural norms and act as a symbol of adulthood to these early emerging adults

Awareness and use of biodiversity collections by fish biologists

A survey of 280 fish biologists from a diverse pool of disciplines was conducted in order to assess the use made of biodiversity collections and how collections can better collect, curate and share the data they have. From the responses, data for how fish biologists use collections, what data they find the most useful, what factors influence the decisions to use collections, how they access the data and explore why some fish biologists make the decision to not use biodiversity collections is collated and reported. The results of which could be used to formulate sustainability plans for collections administrators and staff who curate fish biodiversity collections, while also highlighting the diversity of data and uses to researchers.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/154475/1/jfb14167.pdfhttps://deepblue.lib.umich.edu/bitstream/2027.42/154475/2/jfb14167_am.pd

The Structure of the Star-forming Cluster RCW 38

We present a study of the structure of the high mass star-forming region RCW~38 and the spatial distribution of its young stellar population. Spitzer IRAC photometry 3-8um are combined with 2MASS near-IR data to identify young stellar objects by IR-excess emission from their circumstellar material. Chandra X-ray data are used to identify class III pre-main sequence stars lacking circumstellar material. We identify 624 YSOs: 23 class 0/I and 90 flat spectrum protostars, 437 Class II stars, and 74 Class III stars. We also identify 29 (27 new) O star candidates over the IRAC field. Seventy-two stars exhibit IR-variability, including seven class 0/I and 12 flat spectrum YSOs. A further 177 tentative candidates are identified by their location in the IRAC [3.6] vs. [3.6]-[5.8] cmd. We find strong evidence of subclustering in the region. Three subclusters were identified surrounding the central cluster, with massive and variable stars in each subcluster. The central region shows evidence of distinct spatial distributions of the protostars and pre-main sequence stars. A previously detected IR cluster, DB2001_Obj36, has been established as a subcluster of RCW 38. This suggests that star formation in RCW 38 occurs over a more extended area than previously thought. The gas to dust ratio is examined using the X-ray derived hydrogen column density, N_H and the K-band extinction, and found to be consistent with the diffuse ISM, in contrast with Serpens & NGC1333. We posit that the high photoionising flux of massive stars in RCW 38 affects the agglomeration of the dust grains.Comment: 98 pages, 15 figure

Spitzer Observations of NGC 1333: A Study of Structure and Evolution in a Nearby Embedded Cluster

We present a comprehensive analysis of structure in the young, embedded cluster, NGC 1333 using members identified with Spitzer and 2MASS photometry based on their IR-excess emission. In total, 137 members are identified in this way, composed of 39 protostars and 98 more evolved pre-main sequence stars with disks. Of the latter class, four are transition/debris disk candidates. The fraction of exposed pre-main sequence stars with disks is 83% +/- 11%, showing that there is a measurable diskless pre-main sequence population. The sources in each of the Class I and Class II evolutionary states are shown to have very different spatial distributions relative to the distribution of the dense gas in their natal cloud. However, the distribution of nearest neighbor spacings among these two groups of sources are found to be quite similar, with a strong peak at spacings of 0.045 pc. Radial and azimuthal density profiles and surface density maps computed from the identified YSOs show that NGC 1333 is elongated and not strongly centrally concentrated, confirming previous claims in the literature. We interpret these new results as signs of a low velocity dispersion, extremely young cluster that is not in virial equilibrium.Comment: 59 pages, 20 figures, accepted to ApJ, verion with full resolution figures available at http://www.cfa.harvard.edu/~rgutermuth/preprints/gutermuth_ngc1333.pdf . Updated to fix astro-ph figure garblin

Geologic Evaluation of Critical Production Parameters for Coalbed Methane Resources, Black Warrior Basin - Final Report

Characterization of coalbed methane occurrence and producibility in the Black Warrior basin of Alabama indicates that geologic factors are the principal controls on the occurrence and producibility of coalbed methane. Results of engineering analysis indicate that application of completion and stimulation techniques may be used to increase recovery once favorable well sites are chosen. Sedimentologic and coal-quality parameters may be used to locate regions for coalbed methane development by characterizing the occurrence, rank, and grade of coal resources. However, high-productivity trends within those regions are localized, and geologic data suggest that productivity trends may be predictable on the basis of structural and hydrologic parameters. Several highly productive trends occur along northeast-oriented lineaments. These trends evidently are the surface expression of zones of enhanced permeability which apparently are related to fractures. Productive trends also are associated with areas of low reservoir pressure, and salinity maps indicate that fresh water has migrated toward these areas from the southeast margin of the basin. The available data indicate that structure and hydrology are critical production parameters that may be used to identify favorable well sites within regions containing significant, high-quality coal resources.Bureau of Economic Geolog