169 research outputs found
Combined effect of diluted betadine and Serratia marcescens nuclease on viability of rat hepatoma cells
In order to enhance the tumoricidal efficacy of the diluted Betadine® its water solutions were combined with the buffer solutions of Serratia marcescens nuclease. Examination of the combined effect on viability of cancer cells of rat hepatoma (H4-II-E-C3) in vitro showed the enhancement of a tumoricidal effectiveness of the prepared composition in comparison with the diluted Betadine® or the nuclease solutions. The Betadine® water solutions containing up to 5% of povidone-iodine were found to be compatible with Serratia marcescens nuclease solutions
A One-Step Protocol for Chromatographic Purification of Non-recombinant Exogenous Bacterial Enzyme: Nuclease of Serratia marcescens
© 2016, Springer Science+Business Media New York.Serratia marcescens are well-known Gram-negative bacteria capable of excreting extracellular hydrolases, such as the nuclease—an enzyme that catalyzes hydrolysis of both DNA and RNA chains with a high efficiency. The nuclease has a number of attractive properties for use in biotechnology industry—outstanding enzymatic activity and low production cost. The existing protocols for purification of nuclease yield only limited amounts of protein and require complicated multistage procedures. Here, we report a chromatographic protocol for elegant one-step purification procedure resulting in a pure homogenous enzyme, as confirmed by gel electrophoresis and mass spectrometry
Endonuclease from gram-negative bacteria Serratia marcescens is as effective as pulmozyme in the hydrolysis of DNA in sputum
© 2018 Vafina, Zainutdinova, Bulatov and Filimonova. One of the approaches to effective airway cleansing is the degradation of DNA into smaller fragments. For this purpose Pulmozyme® is used with high efficacy because it contains recombinant DNase I as its active component. The aim of the study was to comparatively analyze DNase activity of Pulmozyme® and the nuclease from gram-negative bacteria Serratia marcescens, because at optimal conditions the catalytic efficiency of the nuclease is much higher than the efficiency of DNase I. Highly polymerized DNA and purulent-mucous sputum were used as substrates. The examination showed that both S. marcescens nuclease and Pulmozyme® hydrolyzed DNA in sputum. Also S. marcescens nuclease was found capable of hydrolyzing DNA in conditions that are standard for Pulmozyme® and suitable for its therapeutic application. For manifesting the similar hydrolytic activity the nuclease amount in the assay mixture containing highly polymerized DNA or the sonicated sputum and NaCl together with calcium- or magnesium- cations can be about 10- time lower than that of the recombinant DNase I. In the presence of magnesium cations the DNase activity of both S. marcescens nuclease and Pulmozyme® was higher than in the presence of calcium cations
IGNITION OF PROPANE-AIR MIXTURE WITH SPATIALLY-NONUNIFORM REACTIVITY, GENERATED BY STREAMER DISCHARGES
This work presents the numerical results regarding to ignition of the premixed lean C3H8-air mixture. The temperature and radical stratifications are created by a filamentary electrical discharge. The formation of combustion wave at the different specific deposited energy and the important role of low temperature oxidation chemistry (below 1000 K) in the preheating zone ahead the flame front for the acceleration of low flame velocity in lean mixture are shown.213-21
Біопрепарати: сучасні перспективи застосування
Topicality. Taking into account the current state of infectious pathology, the emergence of new diseases, changes in biological properties in the already studied pathogens and the burdened course of infectious diseases, as well as taking into account the prevalence and rate of formation of antibiotic resistance, special attention is drawn to the bacteriophages. Control of the prevalence of infectious agents is achieved both through the use of effective antimicrobial agents and immunobiological drugs for prevention purposes.Aim. To substantiate the perspective use of bacteriophages in creating immunoprophylactic agents.Materials and methods. Appelman’s method and the method of double series dilutions were used in the research. Reference strains were used as a microbiological model: S. aureus АТСС 25923, Е. соlі АТСС 25922, В. subtilis АТСС 6633, Р. аеrugenosa 126/18, Pr. mirabilis 128/34, S. abony Dick 1,4,5,12, Str. pyogenes Dick-1.Results and discussion. The conducted researches on studying the virulence of bacteriophages by means of determination of the lytical activity by the method of titration of a bacteriophage in a liquid nutrient medium (Appelman’s method) have established that the virulence of phages is characterized by the ability of directed lysis of morpho-anatomic structure of target bacteria. In addition, the dependence of the level of lithium titers of phages on the tinctorial characteristics of target bacteria was established: Gram-negative bacteria were 2 times less sensitive to the action of phages.Conclusions. Obtained results of researches proved expediency of application of bacteriophages for immunoprophylactic purposes due to absence of side immunochemical effects.Актуальность. Учитывая современное состояние инфекционной патологии, появление новых заболеваний, изменение биологических свойств уже изученных возбудителей, отягощенное течение инфекционных заболеваний, а также распространенность и скорость формирования антибиотикорезистентности, особое внимание ученые уделяют бактериофагам. Контроль распространенности возбудителей инфекции происходит за счет применения как эффективных препаратов антимикробного назначения, так и профилактических иммунобиологических препаратов.Цель. Обоснование перспективности использования бактериофагов для создания иммунопрофилактических средств.Материалы и методы. При проведении исследований использованы методика Аппельмана и метод двукратных серийных разведений. В качестве микробиологической модели были задействованыреференс-штаммы: S. aureus АТСС 25923, Е. соlі АТСС 25922, В. subtilis АТСС 6633, Р. аеrugenosa 126/18, Pr. mirabilis 128/34, S. abony Dick 1,4,5,12, Str. pyogenes Dick-1.Результаты и их обсуждение. Проведенные исследования по изучению вирулентности бактериофагов путем определения литической активности по методу титрования бактериофага в жидкой питательной среде (метод Аппельмана) показали, что вирулентность фагов характеризуется способностью к направленному лизису морфо-анатомической структуры бактерий-мишеней. Установлена зависимость уровня литических титров фагов от тинкториальных характеристик бактерий-мишеней: грамотрицательные бактерии в 2 раза менее чувствительны к действию фагов.Выводы. Полученные результаты исследований доказали целесообразность применения бактериофагов по иммунопрофилактическому назначению за счет отсутствия побочных иммунохимических эффектов.Актуальність. Враховуючи сучасний стан інфекційної патології, появу нових захворювань, зміну біологічних властивостей ужевивчених збудників, обтяжений перебіг інфекційних хвороб,а такожпоширеність та швидкість формування антибіотикорезистентності, особливу увагу вчені звертають на бактеріофаги. Контроль поширеності збудників інфекції відбувається за рахунок застосування як ефективних препаратів антимікробного призначення, так і профілактичних імунобіологічних препаратів. Мета. Обґрунтування перспективності використання бактеріофагів для створення імунопрофілактичних засобів. Матеріалитаметоди. Під час проведення досліджень використовували методику Аппельмана й метод дворазових серійних розведень. Як мікробіологічну модель використовували референс-штами: S. aureus АТСС 25923, Е. соlі АТСС 25922, В. subtilis АТСС 6633, Р. аеrugenosa 126/18, Pr. mirabilis 128/34, S. abony Dick 1,4,5,12, Str. pyogenes Dick-1.Результати та їх обговорення. Проведені дослідження щодо вивчення вірулентності бактеріофагів шляхом визначення літичної активності за методом титрування бактеріофага в рідкому поживному середовищі (метод Аппельмана) засвідчили, що вірулентність фагів характеризується здатністю до спрямованого лізису морфо-анатомічної структури бактерій-мішеней. Крім того, виявлено залежність рівня літичних титрів фагів від тинкторіальних характеристик бактерій-мішеней: грамнегативні бактерії в 2 рази менш чутливі до дії фагів.Висновки. Отримані результати досліджень довели доцільність застосування бактеріофагів за імунопрофілактичним призначенням за рахунок відсутності побічних імунохімічних ефектів
Antioxidative Activity of Ferrocenes Bearing 2,6-Di-Tert-Butylphenol Moieties
The antioxidative activity of ferrocenes bearing either 2,6-di-tert-butylphenol or phenyl groups has been compared using DPPH (1,1-diphenyl-2-picrylhydrazyl) test and in the study of the in vitro impact on lipid peroxidation in rat brain homogenate and on some characteristics of rat liver mitochondria. The results of DPPH test at 20°C show that the activity depends strongly upon the presence of phenolic group but is improved by the influence of ferrocenyl fragment. The activity of N-(3,5-di-tert-butyl-4-hydroxyphenyl)iminomethylferrocene (1), for instance, was 88.4%, which was higher than the activity of a known antioxidant 2,6-di-tert-butyl-4-methylphenol (BHT) (48.5%), whereas the activity of N-phenyl-iminomethylferrocene 2 was almost negligible −2.9%. The data obtained demonstrate that the compounds with 2,6-di-tert-butylphenol moiety are significantly more active than the corresponding phenyl analogues in the in vitro study of lipid peroxidation in rat brain homogenate. Ferrocene 1 performs a promising behavior as an antioxidant and inhibits the calcium-dependent swelling of mitochondria. These results allow us to propose the potential cytoprotective (neuroprotective) effect of ditopic compounds containing antioxidant 2,6-di-tert-butylphenol group and redox active ferrocene fragment
Student Mobility as a Form of Education Internationalization: A Systems Approach to Management
According to the analysis of the existing internationalization strategies it is demonstrated that its main source is an international students’ mobility, which is based on ability, willingness and common interest in cooperation of three following subjects: countries importing education, education-exporting countries, higher educational institution of the exporting country, which provides its educational services for the citizens of the country importing education. The success of this process depends on a certain set of factor indicators in politics, economics, sociology and technology, which are peculiar to each of three participants. The authors propose common to all the participants list of criteria, which evaluate indicators of the stated factors according to the integrated scale: political (administrative) stability, degree of participants’ cooperation, legislation in educational sphere, mutual amiability of the subjects, level of administrative barriers, peculiarities of demographic situation, economic performance, demand for majors of training, logistic accessibility, the number of graduate students, language attraction of training programs, level of career-oriented activities, correspondence between the level of applicants’ acquirements and requirements of the education-exporting country, competition on the educational services market, accessibility of distance educational technologies, development level and dynamics of innovations. The article gives an integral estimation of the efficiency forecast for the work of exporting higher educational institution with the population of a particular education-importing country. This estimation is carried out by means of the analysis of the formed multi-level hierarchical structure. The convolution of hierarchical elements on each level of the hierarchical structure is done using a generalised f-mean, proposed by Kolmogorov – Nagumo. There are given examples of the proposed methodology implication for quantitative and comparative assessment of the potential of education-importing countries. Due to this, international services of the university determine the most attractive countries in terms of international students’ mobility organization and therefore concentration of administrative and financial resources in the chosen direction, in order to make decision on the university brand promotion on the foreign market of educational services. The model can be easily modified by adding the relevant or excluding irrelevant parameters for a given university
A comparative study of Epipactis atrorubens in two different forest communities of the Middle Urals, Russia
The objective of this study was to compare eco-physiological and morphological parameters of a regionally endangered orchid species, Epipactis atrorubens (Hoffm. ex Bernh.) Bess., growing in two forest communities (on serpentine and granite outcrops) of the Middle Urals, Russia. Biodiversity, dominance, and phytocoenosis studies showed the colonization of a wide range of plant species on both sites. The physicochemical properties of the soil, chemical composition and morphological features of E. atrorubens, growing under technogenic conditions (asbestos deposits), on serpentine outcrops and in the natural environment of the granite massif were studied for the first time. The serpentine substrate differed from the granite one by its greater stoniness, circumneutral pH and lower contents of available nitrogen and phosphorus. Extremely high concentrations of magnesium were found in the serpentine soil, some 79 times higher than in the granite substrate. High concentrations of nickel (94 times), chromium (59 times), cobalt (17 times), and iron (4 times) were found in the serpentine substrate, higher than in the granite substrate. The differences between the sites for available metal contents and for root and shoot metal contents were significantly less. Concentrations of most of the metals in the roots were higher than in the shoots. Despite higher metal concentrations and lower nitrogen and phosphorus levels in serpentine soils, E. atrorubens had a larger population and greater viability compared to those growing on granite. Plants on serpentine outcrops were characterized by the formation of a larger number of fruits, greater root lengths and thicker leaf blades, compared to plants on granites. The well-developed orchid mycorrhizae contributed to the survival of this species under unfavorable serpentine conditions. Hence, serpentine outcrops formed due to the mining of asbestos could be a suitable substrate for the light-demanding E. atrorubens due to its capacity to adapt to dry, rocky, nutrient-depleted soils and limited competition from other plants. © 2019, The Author(s).The work was supported by the Ministry of Science and Higher Education of the Russian Federation in the framework of the State Task of UrFU No. 6.7696.2017
Nuclease composition with anti-rabies activity
A composition demonstrating anti-rabies activity was developed on the basis of isoform Sm1 of Serratia marcescens endonuclease. Besides the enzyme the composition contained Haemodes-N, magnesium sulfate and sodium chloride. Injection of this composition at a mouse's infection cite, two hours after the mouse has been infected with rabies, showed an increase of the infected animals' survival by more than 30% in comparison with the animals of control group
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