Evidence for uteroplacental malperfusion in fetuses with major congenital heart defects.

AIMS: Fetuses affected by congenital heart defects (CHD) are considered to be at increased risk of fetal growth restriction and intrauterine demise. Whether these risks are a direct consequence of fetal CHD or a result of associated uteroplacental dysfunction is not evident from the data of recent studies. The aim of this study was to investigate the prevalence of uteroplacental dysfunction reflected by abnormal uterine artery Doppler indices and reduced fetal growth in CHD pregnancies. METHODS: This is a retrospective case-control study including singleton pregnancies referred for detailed fetal cardiac assessment subsequently diagnosed with or without CHD. Mid-trimester uterine artery Doppler assessment at 20-24 weeks as well as third trimester fetal biometry and arterial Doppler pulsatility indices (PI) were performed. All fetal biometry were converted into centiles and Doppler values to multiples of median (MoM) to adjust for physiological changes with gestation. RESULTS: The study included 811 pregnancies including 153 cases where the fetus was diagnosed with CHD. Mid-pregnancy uterine artery PI was significantly higher in women with fetal CHD compared to controls (0.90MoM vs 0.83MoM; p = 0.006). In the third trimester, median centiles for fetal head circumference (45.4 vs 57.07; p<0.001), abdominal circumference (51.17 vs 55.71; p = 0.014), estimated fetal weight (33.6 vs 56.7; p<0.001) and cerebroplacental ratio (CPR: 0.84MoM vs 0.95MoM; p<0.001) were significantly lower in fetuses with CHD compared to controls. The percentage of small for gestational age births <10th centile (24.0% vs 10.7%; <0.001) and low CPR <0.6MoM (11.7% vs 2.5%; p<0.001) were significantly higher in the fetal CHD cohort. CONCLUSIONS: Mid-pregnancy uterine artery resistance is increased and subsequent fetal biometry reduced in pregnancies with CHD fetuses. These findings suggest that fetal CHD are associated with uteroplacental dysfunction, secondary to impaired maternal uteroplacental perfusion resulting in relative fetal hypoxaemia and reduced fetal growth

Manejo e controle de plantas infestantes em fruteiras tropicais.

A produção agrícola brasileira é a cada dia demandada para oferecer produtos de qualidade ao consumidor, produzidos por sistemas de produção agrícolas economicamente viáveis, energeticamente eficientes e ecologicamente sustentáveis que protejam e conservem os recursos naturais e o meio ambiente.Palestra

Using the R Package crlmm for Genotyping and Copy Number Estimation

Genotyping platforms such as Affymetrix can be used to assess genotype-phenotype as well as copy number-phenotype associations at millions of markers. While genotyping algorithms are largely concordant when assessed on HapMap samples, tools to assess copy number changes are more variable and often discordant. One explanation for the discordance is that copy number estimates are susceptible to systematic differences between groups of samples that were processed at different times or by different labs. Analysis algorithms that do not adjust for batch effects are prone to spurious measures of association. The R package crlmm implements a multilevel model that adjusts for batch effects and provides allele-specific estimates of copy number. This paper illustrates a workflow for the estimation of allele-specific copy number and integration of the marker-level estimates with complimentary Bioconductor software for inferring regions of copy number gain or loss. All analyses are performed in the statistical environment R.

A time of flight method to measure the speed of sound using a stereo sound card

We present an inexpensive apparatus for measuring the speed of sound, with a time of flight method, using a computer with a stereo sound board. Students measure the speed of sound by timing the delay between the arrivals of a pulse to two microphones placed at different distances from the source. It can serve as a very effective demonstration, providing a quick measurement of the speed of sound in air; we have used it with great success in Open Days in our Department. It can also be used for a full fledged laboratory determination of the speed of sound in air.Comment: Accepted for publication in The Physics Teache

Polarimetry of Compact Symmetric Objects

We present multi-frequency VLBA observations of two polarized Compact Symmetric Objects (CSOs), J0000+4054 and J1826+1831, and a polarized CSO candidate, J1915+6548. Using the wavelength-squared dependence of Faraday rotation, we obtained rotation measures (RMs) of -180 \pm 10 rad m^-2 and 1540 \pm 7 rad m^-2 for the latter two sources. These are lower than what is expected of CSOs (several 1000 rad m^-2) and, depending on the path length of the Faraday screens, require magnetic fields from 0.03 to 6 \mu G. These CSOs may be more heavily affected by Doppler boosting than their unpolarized counterparts, suggesting that a jet-axis orientation more inclined towards the line of sight is necessary to detect any polarization. This allows for low RMs if the polarized components are oriented away from the depolarizing circumnuclear torus. These observations also add a fourth epoch to the proper motion studies of J0000+4054 and J1826+1831, constraining their kinematic age estimates to >610 yrs and 2600 \pm 490 yrs, respectively. The morphology, spectrum, and component motions of J1915+6548 are discussed in light of its new classification as a CSO candidate, and its angle to the line of sight (~50\deg) is determined from relativistic beaming arguments.Comment: 29 pages, including 9 figures; Accepted by Astrophysical Journal, 16 Feb 0

Comparação entre espécies florestais para repovoamento.

bitstream/item/101241/1/PA-1983-Carvalho-ComparacaoEspecies.pd

Plasmon polaritons in photonic superlattices containing a left-handed material

We analyze one-dimensional photonic superlattices which alternate layers of air and a left-handed material. We assume Drude-type dispersive responses for the dielectric permittivity and magnetic permeability of the left-handed material. Maxwell's equations and the transfer-matrix technique are used to derive the dispersion relation for the propagation of obliquely incident optical fields. The photonic dispersion indicates that the growth-direction component of the electric (or magnetic) field leads to the propagation of electric (or magnetic) plasmon polaritons, for either TE or TM configurations. Furthermore, we show that if the plasma frequency is chosen within the photonic $=0$ zeroth-order bandgap, the coupling of light with plasmons weakens considerably. As light propagation is forbidden in that particular frequency region, the plasmon-polariton mode reduces to a pure plasmon mode.Comment: 4 pages, 4 figure

Protocolo modificado para extração de DNA do gênero Pestalotiopsis.

Objetivou-se com este trabalho modificar o protocolo de extração de DNA do gênero Pestalotiopsis. Foram obtidos 200 mg de micélio de 11 isolados de Pestalotiopsis sp. O protocolo de extração proposto por Joshi (2009) foi modificado e testado retirando-se o fenol no processo de desproteinização do DNA. A quantidade de DNA genômico extraído foi quantificada em fotodocumentador e a qualidade do DNA final foi avaliada em gel 0,9% de agarose e em reações de marcadores RAPD. A metodologia sem o emprego do fenol proporcionou quantidade satisfatória (32,55 a 49,92 ng/µL) e pureza de DNA para caracterização molecular do fungo. O protocolo modificado, portanto, pode ser utilizado para extrair DNA do gênero Pestalotiopsis com eficiência do método e segurança para os usuários

A importância do controle de qualidade dos inoculantes.

bitstream/item/71827/1/ID-30961.pd