2,334 research outputs found
Human SULT1A genes: Cloning and activity assays of the SULT1A promoters
The three human SULT1A sulfotransferase enzymes are closely related in amino acid sequence (>90%), yet differ in their substrate preference and tissue distribution. SULT1A1 has a broad tissue distribution and metabolizes a range of xenobiotics as well as endogenous substrates such as estrogens and iodothyronines. While the localization of SULT1A2 is poorly understood, it has been shown to metabolize a number of aromatic amines. SULT1A3 is the major catecholamine sulfonating form, which is consistent with it being expressed principally in the gastrointestinal tract. SULT1A proteins are encoded by three separate genes, located in close proximity to each other on chromosome 16. The presence of differential 5′-untranslated regions identified upon cloning of the SULT1A cDNAs suggested the utilization of differential transcriptional start sites and/or differential splicing. This chapter describes the methods utilized by our laboratory to clone and assay the activity of the promoters flanking these different untranslated regions found on SULT1A genes. These techniques will assist investigators in further elucidating the differential mechanisms that control regulation of the human SULT1A genes. They will also help reveal how different cellular environments and polymorphisms affect the activity of SULT1A gene promoters
Proton NMR measurements of the local magnetic field in the paramagnetic metal and antiferromagnetic insulator phases of -(BETS)FeCl
Measurements of the H-NMR spectrum of a small ( 4 g) single
crystal of the organic conductor -(BETS)FeCl are reported
with an applied magnetic field = 9 T parallel to the a-axis in
the -plane over a temperature range 2.0 180 K. They provide the
distribution of the static local magnetic field at the proton sites in the
paramagnetic metal (PM) and antiferromagnetic insulator (AFI) phases, along
with the changes that occur at the PMAFI phase transition. The spectra have
six main peaks that are significantly broadened and shifted at low . The
origin of these features is attributed to the large dipolar field from the 3d
Fe ion moments (spin = 5/2). Their amplitude and
dependence are modeled using a modified Brillouin function that includes a
mean field approximation for the total exchange interaction () between
one Fe ion and its two nearest neighbors. A good fit is obtained using
= 1.7 K. At temperatures below the PMAFI transition temperature
= 3.5 K, an extra peak appears on the high frequency side of the
spectrum and the details of the spectrum become smeared. Also, the rms
linewidth and the frequency shift of the spectral distribution are
discontinuous, consistent with the transition being first-order. These
measurements verify that the dominant local magnetic field contribution is from
the Fe ions and indicate that there is a significant change in the
static local magnetic field distribution at the proton sites on traversing the
PM to AFI phase transition.Comment: 11 pages, 7 figures. Revised version of cond-mat/0605044 resubmitted
to Phys. Rev. B in response to comments of Editor and reviewer
The Negishi Catalysis: Full Study of the Complications in the Transmetalation Step and Consequences for the Coupling Products
Herschel PACS Spectroscopic Diagnostics of Local ULIRGs: Conditions and Kinematics in Mrk 231
In this first paper on the results of our Herschel PACS survey of local
Ultraluminous Infrared Galaxies (ULIRGs), as part of our SHINING survey of
local galaxies, we present far-infrared spectroscopy of Mrk 231, the most
luminous of the local ULIRGs, and a type 1 broad absorption line AGN. For the
first time in a ULIRG, all observed far-infrared fine-structure lines in the
PACS range were detected and all were found to be deficient relative to the far
infrared luminosity by 1 - 2 orders of magnitude compared with lower luminosity
galaxies. The deficits are similar to those for the mid-infrared lines, with
the most deficient lines showing high ionization potentials. Aged starbursts
may account for part of the deficits, but partial covering of the highest
excitation AGN powered regions may explain the remaining line deficits. A
massive molecular outflow, discovered in OH and 18OH, showing outflow
velocities out to at least 1400 km/sec, is a unique signature of the clearing
out of the molecular disk that formed by dissipative collapse during the
merger. The outflow is characterized by extremely high ratios of 18O / 16O
suggestive of interstellar medium processing by advanced starbursts.Comment: Accepted for publication in the Astronomy and Astrophysics Herschel
Special Issue, 5 pages, 4 figure
The Error and Repair Catastrophes: A Two-Dimensional Phase Diagram in the Quasispecies Model
This paper develops a two gene, single fitness peak model for determining the
equilibrium distribution of genotypes in a unicellular population which is
capable of genetic damage repair. The first gene, denoted by ,
yields a viable organism with first order growth rate constant if it
is equal to some target ``master'' sequence . The second
gene, denoted by , yields an organism capable of genetic repair
if it is equal to some target ``master'' sequence . This
model is analytically solvable in the limit of infinite sequence length, and
gives an equilibrium distribution which depends on \mu \equiv L\eps , the
product of sequence length and per base pair replication error probability, and
\eps_r , the probability of repair failure per base pair. The equilibrium
distribution is shown to exist in one of three possible ``phases.'' In the
first phase, the population is localized about the viability and repairing
master sequences. As \eps_r exceeds the fraction of deleterious mutations,
the population undergoes a ``repair'' catastrophe, in which the equilibrium
distribution is still localized about the viability master sequence, but is
spread ergodically over the sequence subspace defined by the repair gene. Below
the repair catastrophe, the distribution undergoes the error catastrophe when exceeds \ln k/\eps_r , while above the repair catastrophe, the
distribution undergoes the error catastrophe when exceeds , where denotes the fraction of deleterious mutations.Comment: 14 pages, 3 figures. Submitted to Physical Review
Cationic Alkylaluminum-Complexed Zirconocene Hydrides as Participants in Olefin Polymerization Catalysis
A Survey of HC3N in Extragalactic Sources - Is HC3N a Tracer of Activity in ULIRGs?
Context. HC3N is a molecule that is mainly associated with Galactic
star-forming regions, but it has also been detected in extragalactic
environments. Aims. To present the first extragalactic survey of HC3N, when
combining earlier data from the literature with six new single-dish detections,
and to compare HC3N with other molecular tracers (HCN, HNC), as well as other
properties (silicate absorption strength, IR flux density ratios, C II flux,
and megamaser activity). Methods. We present mm IRAM 30 m, OSO 20 m, and SEST
observations of HC3N rotational lines (mainly the J = 10-9 transition) and of
the J = 1-0 transitions of HCN and HNC. Our combined HC3N data account for 13
galaxies (excluding the upper limits reported for the non-detections), while we
have HCN and HNC data for more than 20 galaxies. Results. A preliminary
definition "HC3N-luminous galaxy" is made based upon the HC3N/HCN ratio. Most
(~80 %) HC3N-luminous galaxies seem to be deeply obscured galaxies and
(U)LIRGs. A majority (~60 % or more) of the HC3N-luminous galaxies in the
sample present OH mega- or strong kilomaser activity. A possible explanation is
that both HC3N and OH megamasers need warm dust for their excitation.
Alternatively, the dust that excites the OH megamaser offers protection against
UV destruction of HC3N. A high silicate absorption strength is also found in
several of the HC3N-luminous objects, which may help the HC3N to survive.
Finally, we find that a high HC3N/HCN ratio is related to a high dust
temperature and a low C II flux.Comment: 17 pages, 12 figures, to be published in Astronomy & Astrophysic
Role of PTGS2-generated PGE2 during gonadotrophin-induced bovine oocyte maturation and cumulus cell expansion
Versatile Coordination of Cyclopentadienyl-Arene Ligands and Its Role in Titanium-Catalyzed Ethylene Trimerization
Cationic titanium(IV) complexes with ansa-(η5-cyclopentadienyl,η6-arene) ligands were synthesized and characterized by X-ray crystallography. The strength of the metal-arene interaction in these systems was studied by variable-temperature NMR spectroscopy. Complexes with a C1 bridge between the cyclopentadienyl and arene moieties feature hemilabile coordination behavior of the ligand and consequently are active ethylene trimerization catalysts. Reaction of the titanium(IV) dimethyl cations with CO results in conversion to the analogous cationic titanium(II) dicarbonyl species. Metal-to-ligand backdonation in these formally low-valent complexes gives rise to a strongly bonded, partially reduced arene moiety. In contrast to the η6-arene coordination mode observed for titanium, the more electron-rich vanadium(V) cations [cyclopentadienyl-arene]V(NiPr2)(NC6H4-4-Me)+ feature η1-arene binding, as determined by a crystallographic study. The three different metal-arene coordination modes that we experimentally observed model intermediates in the cycle for titanium-catalyzed ethylene trimerization. The nature of the metal-arene interaction in these systems was studied by DFT calculations.
Dielectric ordering and colossal magnetodielectricity in the antiferromagnetic insulating state of λ-(BEDT-TSF)2FeCl4
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