Biomedical Applications and Syntheses of Selected Anthraquinone Dyes

Anthraquinones are aromatic organic compounds that have multiple applications in the biomedical field. Some anthraquinone-based compounds are used as fluorophores to contrast cell nuclei while others act as chemotherapeutic agents. However, there are not many fluorescent anthraquinone cell stains currently available. In this study, commercially available anthraquinone dyes, in addition to other dye families and compounds, were reviewed for their unique properties, advantages, and drawbacks. The development and characterization of three novel anthraquinone fluorophores revealed promising photophysical characteristics, like large Stokes shifts. One of the compounds, RBS3, was chosen for fixed and live cell staining and exhibited desirable biomedical properties. RBS3 is highly cell permeable, appears to be photostable, does not require a wash-off procedure, and stains the nucleus, cytoplasm, and endoplasmic reticulum

Impact of Rainfall on Coliform and Parasite Levels in Local Groundwater

Undergraduate Basi

Savoir comment faire pour enseigner les savoir-faire

Une équipe de professeurs et professionnels du Collège Montmorency s’est concertée pour peaufiner une méthode permettant d’enseigner plus efficacement les savoir-faire pratiques dans les classes de sciences. En plus de renforcer le sentiment de responsabilité des étudiants face à l’apprentissage, celle-ci vise, entre autres, à leur assurer une plus grande maitrise de diverses connaissances et compétences procédurales. Une approche didactique, une marche à suivre comportant quatre étapes et des outils d’accompagnement, de vérification et de correction ont été développés pour favoriser la cohérence du travail des professeurs et des techniciens et pour leur permettre d’allouer plus de temps à l’enseignement pratique, essentiel dans leur discipline. Il faut lire cet article stimulant pour en savoir plus et se laisser inspirer

The Physical Activity Tracker Testing in Youth (P.A.T.T.Y.) Study: Content Analysis and Children\u27s Perceptions

Background: Activity trackers are widely used by adults and several models are now marketed for children. Objective: The aims of this study were to (1) perform a content analysis of behavioral change techniques (BCTs) used by three commercially available youth-oriented activity trackers and (2) obtain feedback describing children’s perception of these devices and the associated websites. Methods: A content analysis recorded the presence of 36 possible BCTs for the MovBand (MB), Sqord (SQ), and Zamzee (ZZ) activity trackers. In addition, 16 participants (mean age 8.6 years [SD 1.6]; 50% female [8/16]) received all three trackers and were oriented to the devices and websites. Participants were instructed to wear the trackers on 4 consecutive days and spend ≥10 min/day on each website. A cognitive interview and survey were administered when the participant returned the devices. Qualitative data analysis was used to analyze the content of the cognitive interviews. Chi-square analyses were used to determine differences in behavioral monitoring and social interaction features between websites. Results: The MB, SQ, and ZZ devices or websites included 8, 15, and 14 of the possible 36 BCTs, respectively. All of the websites had a behavioral monitoring feature (charts for tracking activity), but the percentage of participants indicating that they “liked” those features varied by website (MB: 8/16, 50%; SQ: 6/16, 38%; ZZ: 11/16, 69%). Two websites (SQ and ZZ) included an “avatar” that the user could create to represent themselves on the website. Participants reported that they “liked” creating and changing their avatar (SQ: 12/16, 75%, ZZ: 15/16, 94%), which was supported by the qualitative analyses of the cognitive interviews. Most participants (75%) indicated that they would want to wear the devices more if their friends were wearing a tracker. No significant differences were observed between SQ and ZZ devices in regards to liking or use of social support interaction features (P=.21 to .37). Conclusions: The websites contained several BCTs consistent with previously identified strategies. Children “liked” the social aspects of the websites more than the activity tracking features. Developers of commercial activity trackers for youth may benefit from considering a theoretical perspective during the website design process

Objective Analysis of Preschoolers Physical Activity Patterns during Free Playtime

PURPOSE: Preschool-age children are not meeting the recommended levels of daily physical activity (PA), leading states to implement policies requiring preschools to provide students with 30-60 minutes of free playtime. Preschoolers’ activity patterns are intermittent nature. It is possible that current PA policies are not conducive in increasing PA in preschoolers. Currently, there is no data on the temporal patterns of PA intensities during preschoolers’ free playtime. The purpose of this study was to examine the temporal patterns of preschoolers’ PA levels during a typical 30-minute playtime. METHODS: Baseline playtime accelerometer counts from three preschool intervention studies were utilized (n=363, age=4.0±0.8 years). Thirty minutes of data were collected using 15-second epochs and classified into sedentary, light and moderate-to-vigorous intensity, and averaged in 5-minute blocks [B1 (0-5minute)-B6 (25-30minute)]. Differences between blocks were analyzed using repeated-measures ANOVA, with a Bonferroni correction for pairwise comparisons. RESULTS: There were significant main effects for percent epochs in sedentary (F (5, 1625)=3.73, p=0.0003), light (F (5, 1625)=2.65, p=0.02) and MVPA (MVPA, F (5, 1625)=2.87, p CONCLUSIONS: The present data suggest that PA strategies should be implemented to improve the activity level of preschoolers within the first and last 10-minutes of a 30-min playtime or providing more frequent, but shorter playtimes. Additional research is needed to understand the play patterns of preschoolers during shorter/longer playtimes

Knowing how to teach know-how : imparting procedural knowledge

Comprend des réréfences bibliographiques

The Quest for the Ideal Fluorescent Cell Stain

Undergraduate Applie

Design and Analysis of a Novel Fluorescent Cell Stain

Undergraduate Basi

Medical tourism: the role of the primary care provider

El artículo aborda el tema del turismo médico, siendo esta una alternativa muchas veces rentable, pero que involucra importantes riesgos para los pacientes que eligen esta opción