Genetic variation among Northern and Southern Egyptian buffaloes using polymerase chain reaction-random amplified polymorphic DNA (PCR-RAPD)

The domestic water buffalo is a species of great economic potential, especially in developing countries like Egypt. Egyptian buffalo have been classified according to minor phenotypic differences and their geographical locations. Few studies have taken place to investigate the genetic variations in Egyptian buffalo using  microsatellites analysis. In the present study, 11 random primers were analyzed for the genetic diversity  determination between Northern and Southern Egyptian buffaloes using polymerase chain reaction-random  amplified polymorphic DNA (PCR-RAPD) analysis. 169 bands were amplified for the analyzed 11 random  primers, from which 160 bands (94.67%) for North populations and 168 bands for South population (99.41%).  Out of the 160 amplified bands in North populations, 152 bands were polymorphic with a percentage of 89.94% and only one specific band (0.59%). In South population, all 168 amplified bands were polymorphic, nine bands (5.33%) were specific for this population. The identity index and the genetic distance between North and South populations were measured. The results showed that the two tested populations have the same origin and  belong to one breed without significant genetic difference between their animals.Key words: Buffalo, genetic diversity, polymerase chain reaction (PCR), random amplified polymorphic DNA (RAPD)

PENINGKATAN KECAKAPAN MASYARAKAT SONAF HONIS BONEN DALAM BUDIDAYA DAN PENGOLAHAN CABAI

Ketersediaan buah cabai di pasaran umumnya tidak stabil. Saat panen raya suplai cabai melimpah sehingga harganya rendah. Sebaliknya saat musim hujan, suplai cabai sangat terbatas sehingga harganya melonjak. Masyarakat desa Oeltua Kabupaten Kupang umumnya adalah petani dan peternak. Salah satu tanaman yang banyak diusahakan adalah cabai. Melalui kegiatan PKM ini dilakukan transfer ilmu pengetahuan dan keterampilan bagi Kelompok Tani Kaum Ibu Sonaf Honis Bonen dan masyarakat setempat untuk meningkatkan kecakapan dalam budidaya cabai serta pengolahan cabai, yaitu dengan pemanfaatan mulsa plastik hitam perak serta penggunaan food dehydrator dalam pengolahan buah cabai. Kegiatan dilakukan dengan metode penyuluhan dan diskusi serta demplot penggunaan mulsa plastik dalam budidaya cabai, serta praktek pengolahan buah cabai menggunakan food dehydrator. Mulsa plastik berguna untuk mencegah pertumbuhan gulma serta mengurangi evaporasi. Hal ini sesuai untuk Kabupaten Kupang yang termasuk daerah semi arid atau beriklim kering. Proses pengeringan buah cabai cukup sederhana sehingga mudah dipraktekkan oleh peserta kegiatan ini. Luaran kegiatan PKM ini berupa produk buah cabai kering, boncabai, serta serpihan cabai atau chilli-flake. Luaran akademik berupa publikasi ilmiah pada jurnal nasional terindeks Sinta-5, publikasi pada media lokal online serta visdeo kegiatan yang diunggah pada web-Fakultas Pertanian Undana

Prognostic Impact of Previous Percutaneous Coronary Intervention on the Outcome of Coronary Artery Bypass Grafting in Multivessel Disease Diabetic Patients

Background: Previous studies suggest that patients who receive percutaneous coronary intervention (PCI) are at a higher risk of undergoing coronary artery bypass grafting (CABG). This study aimed to investigate the risk of CABG in patients with a history of PCI. Methods: One hundred diabetic patients who underwent CABG from October 2020 to February 2022 were enrolled and divided into two groups. Group I consisted of 50 patients with no prior PCI, while Group II comprised 50 patients with a history of PCI. Results: The mean age was 57.4 ± 8.67 years for Group I and 59.72 ± 7.5 years for Group II (p= 0.155). The mean cardiopulmonary bypass time was 108.56 ± 34.53 minutes for Group I and 127.4 ± 35.93 minutes for Group II (p=0.009). The ischemic duration was 75.68 ± 19.94 minutes for Group I and 75.12± 23.02 minutes for Group II. The mean number of grafts was greater in Group I (3.5 (3 – 3.5) vs. 3 (2 – 4), p= 0.011). The mean ventilation time was 9 (5 – 13.75) hours for Group I and 10 (5 – 19) hours for Group II. The mean length of ICU stay was 1 (1–2) day for Group I and 2 (2–3) days for Group II (p<0.001). The length of hospital stay was 8 (7–9) days for Group I and 10 (9–11) days for Group II (p<0.001). There were statistically significant differences between the groups in terms of MACE (higher in the PCI group, p=0.046), improvement in wall motion abnormalities (higher in the non-PCI group, p=0.007), and postoperative normal ejection fraction (higher in the non-PCI group, p=0.032). There was no significant difference in hospital mortality between the two groups (0 vs 3), with a p value =0.07. Conclusion: A previous PCI could increase post-CABG morbidity and MACEs. However, no significant difference in postoperative mortality rates was found between patients who underwent prior PCI and those who did not

Surgical treatment of rectal prolapse: experience and late results with 51 patients

The "best" surgical technique for the management of complete rectal prolapse remains unknown. Due to its low incidence, it is very difficult to achieve a representative number of cases, and there are no large prospective randomized trials to attest to the superiority of one operation over another. PURPOSE: Analyze the results of surgical treatment of complete rectal prolapse during 1980 and 2002. METHOD: Retrospective study. RESULTS: Fifty-one patients underwent surgical treatment during this period. The mean age was 56.7 years, with 39 females. Besides the prolapse itself, 33 patients complained of mucous discharge, 31 of fecal incontinence, 14 of constipation, 17 of rectal bleeding, and 3 of urinary incontinence. Abdominal operations were performed in 36 (71%) cases. Presacral rectopexy was the most common abdominal procedure (29 cases) followed by presacral rectopexy associated with sigmoidectomy (5 cases). The most common perineal procedure was perineal rectosigmoidectomy associated with levatorplasty (12 cases). Intraoperative bleeding from the presacral space developed in 2 cases, and a rectovaginal fistula occurred in another patient after a perineal rectosigmoidectomy. There were 2 recurrences after a mean follow-up of 49 months, which were treated by reoperation. CONCLUSION: Abdominal and perineal procedures can be used to manage complete rectal prolapse with safety and good long-term results. Age, associated medical conditions, and symptoms of fecal incontinence or constipation are the main features that one should bear in mind in order to choose the best surgical approach.A técnica cirúrgica mais apropriada para a correção da procidência retal permanece motivo de controvérsia. Por se tratar de afecção pouco freqüente, há dificuldade de avaliação de número adequado de pacientes em estudos randomizados e existe pouca evidência para comprovar a superioridade de alguma das técnicas. OBJETIVO: Analisar os resultados de eficácia e segurança do tratamento cirúrgico da procidência retal em pacientes operados entre 1980 e 2002. MÉTODO: Estudo retrospectivo. RESULTADOS: Cinqüenta e um pacientes foram operados. A idade média foi de 56,7 anos e 39 eram mulheres. Além do prolapso, 33 pacientes queixavam-se de eliminação de muco, 31 tinham incontinência anal, 14 apresentavam constipação, 17 com sangramento retal e 3 incontinência urinária. Operações abdominais foram realizadas em 36 (71%) casos, sendo a retopexia sem prótese a operação mais realizada (29 casos) seguida pela retossigmoidectomia com retopexia (5 casos). A operação perineal mais realizada foi a retossigmoidectomia com plastia dos elevadores (12 casos). O sangramento sacral foi a única complicação intra-operatória e ocorreu em dois casos. Como complicação pós-operatória, houve um caso de fístula retovaginal após operação de retossigmoidectomia perineal. Após seguimento médio de 49 meses, observamos recidiva da procidência em 2 casos. CONCLUSÕES: Operações abdominais e perineais podem ser utilizadas com segurança e eficácia no tratamento cirúrgico da procidência do reto. A idade, a presença de afecções associadas, comorbidades e os sintomas de constipação e incontinência são as principais variáveis envolvidas na escolha da operação. As operações de retopexia abdominal e retossigmoidectomia perineal estão associadas a bons resultados

Graded Contractions of Affine Kac-Moody Algebras

The method of graded contractions, based on the preservation of the automorphisms of finite order, is applied to the affine Kac-Moody algebras and their representations, to yield a new class of infinite dimensional Lie algebras and representations. After the introduction of the horizontal and vertical gradings, and the algorithm to find the horizontal toroidal gradings, I discuss some general properties of the graded contractions, and compare them with the In\"on\"u-Wigner contractions. The example of $\hat A_2$ is discussed in detail.Comment: 23 pages, Ams-Te

Interleaved Transcranial Magnetic Stimulation/Functional MRI Confirms that Lamotrigine Inhibits Cortical Excitability in Healthy Young Men

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Pomegranate (Punica graantum) Peels as an Agricultural Waste for Removing of CD(II), CR(VI), CU(II), NI(II), PB(II) and ZN(II) from Their Aqueous Solutions

Pomegranate (Punica graantum) peels as an agricultural waste was used as an adsorbent for removal of Cd, Cr, Cu, Ni, Pb and Zn ions from simulated aqueous solutions. The adsorption process was carried out using the batch method. Various effective parameters such as pH, initial metal ion concentration, and adsorbent dose, shaking time, particle size and temperature were investigated. Fourier Transform Infrared (FTIR) and Scanning Electron Microscopy (SEM) of Punica graantum peels were done. The efficiency of Punica graantum peels toward removal of metal ions was ordered as Pb2+ \u3c Cr6+ \u3c Cu2+ \u3c Cd2+ \u3c Zn2+ \u3c Ni2+, with the corresponding values of 92.8%, 84.6%, 52.8%, 38%, 25.4% and 22.8%, respectively

Biallelic mutations in valyl-tRNA synthetase gene VARS are associated with a progressive neurodevelopmental epileptic encephalopathy.

Aminoacyl-tRNA synthetases (ARSs) function to transfer amino acids to cognate tRNA molecules, which are required for protein translation. To date, biallelic mutations in 31 ARS genes are known to cause recessive, early-onset severe multi-organ diseases. VARS encodes the only known valine cytoplasmic-localized aminoacyl-tRNA synthetase. Here, we report seven patients from five unrelated families with five different biallelic missense variants in VARS. Subjects present with a range of global developmental delay, epileptic encephalopathy and primary or progressive microcephaly. Longitudinal assessment demonstrates progressive cortical atrophy and white matter volume loss. Variants map to the VARS tRNA binding domain and adjacent to the anticodon domain, and disrupt highly conserved residues. Patient primary cells show intact VARS protein but reduced enzymatic activity, suggesting partial loss of function. The implication of VARS in pediatric neurodegeneration broadens the spectrum of human diseases due to mutations in tRNA synthetase genes