Further improvements in the sea urchin embryo cryopreservation protocol (Paracentrotus lividus)

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Comparative cryopreservation study of trochophore larvae from two species of bivalves: Pacific oyster (Crassostrea gigas) and Blue mussel (Mytilus galloprovincialis)

Oysters and mussels are among the most farmed species in aquaculture industry around the world. The aim of this study was to test the toxicity of cryoprotective agents to trochophore larvae from two different species of bivalves and develop an improved cryopreservation protocol to ensure greater efficiency in the development of cryopreserved trochophores (14 h old oyster larvae and 20 h old mussel larvae) to normal D-larvae for future developments of hatchery spat production. The cryopreservation protocol producing the best results for oyster trochophores (60.0 ± 6.7% normal D-larvae) was obtained by holding at 0 °C for 5 min then cooling at 1 °C min(-1) to -10 °C and holding for 5 min before cooling at 0.5 °C to -35 °C, holding 5 min and then plunging into liquid nitrogen (LN), using 10% ethylene glycol. For mussel experiments, no significant differences were found when cooling at 0.5 °C min(-1) or at 1 °C min(-1) for CPA combinations with 10% ethylene glycol and at 0.5 °C min(-1). Using these combinations, around half of trochophores were able to develop to normal D-larvae post-thawing (48.9 ± 7.6% normal D-larvae).Post-print

Normative Emotional Agents: a viewpoint paper

[EN] Human social relationships imply conforming to the norms, behaviors and cultural values of the society, but also socialization of emotions, to learn how to interpret and show them. In multiagent systems, much progress has been made in the analysis and interpretation of both emotions and norms. Nonetheless, the relationship between emotions and norms has hardly been considered and most normative agents do not consider emotions, or vice-versa. In this article, we provide an overview of relevant aspects within the area of normative agents and emotional agents. First we focus on the concept of norm, the different types of norms, its life cycle and a review of multiagent normative systems. Secondly, we present the most relevant theories of emotions, the life cycle of an agent¿s emotions, and how emotions have been included through computational models in multiagent systems. Next, we present an analysis of proposals that integrate emotions and norms in multiagent systems. From this analysis, four relationships are detected between norms and emotions, which we analyze in detail and discuss how these relationships have been tackled in the reviewed proposals. Finally, we present a proposal for an abstract architecture of a Normative Emotional Agent that covers these four norm-emotion relationships.This work was supported by the Spanish Government project TIN2017-89156- R, the Generalitat Valenciana project PROMETEO/2018/002 and the Spanish Goverment PhD Grant PRE2018-084940.Argente, E.; Del Val, E.; Pérez-García, D.; Botti Navarro, VJ. (2022). Normative Emotional Agents: a viewpoint paper. IEEE Transactions on Affective Computing. 13(3):1254-1273. https://doi.org/10.1109/TAFFC.2020.3028512S1254127313

Guía para la localización de fuentes relativas al País Vasco en el Archivo Secreto Vaticano [RESEÑA]

Reseña de "Valeria BEOLCHINI – Marta PAVÓN RAMÍREZ, Guía para la localización de fuentes relativas al País Vasco en el Archivo Secreto Vaticano. Documentación medieval. Díaz de Durana, José Ramón; Jular Pérez-Alfaro, Cristina (coords.), Universidad del País Vasco, Bilbao 2014, 91 pp." y "Marta PAVÓN RAMÍREZ, Guía para la localización de fuentes relativas al País Vasco en el Archivo Secreto Vaticano. Documentación de época moderna (1458-1830) Porres Marijuán, Rosario et al. (coords.), Universidad del País Vasco, Bilbao 2014, 121 pp.

Discursive synergies for a 'Great Transformation' towards sustainability : pragmatic contributions to a necessary dialogue between human development, degrowth, and buen vivir

Unidad de excelencia María de Maeztu MdM-2015-0552There is a growing awareness that a whole-societal " Great Transformation " of Polanyian scale is needed to bring global developmental trajectories in line with ecological imperatives. The mainstream Sustainable Development discourse, however, insists in upholding the myth of compatibility of current growth-based trajectories with biophysical planetary boundaries. This article explores potentially fertile complementarities among trendy discourses challenging conventional notions of (un)sustainable development - Human Development, Degrowth, and Buen Vivir - and outlines pathways for their realization. Human Development presents relative transfor-mative strengths in political terms, while Degrowth holds keys to unlocking unsustainable material-structural entrenchments of contemporary socioeconomic arrangements, and Buen Vivir offers a space of cultural alterity and critique of the Euro-Atlantic cultural constellation. The weaknesses or blind spots ('Achilles heels') of each discourse can be compensated through the strengths of the other ones, creating a dialogical virtuous circle that would open pathways towards a global new " Great Transformation ". As one of the main existing platforms for pluralist and strong-sustainability discussions, Ecological Economics is in a privileged position to deliberately foster such strategic discursive dialogue. A pathway towards such dialogue is illuminated through a model identifying and articulating key discursive docking points

Antiagregación y anticoagulación en síndromes coronarios agudos: niveles de evidencia

Management of acute coronary syndromes (ACS) has moved rapidly in parallel with our understanding of the pathophysiological basis of the disease. In the eighties, the demonstration of the pivotal role of coronary thrombosis in the etiology of a ACS led to administration of aspirin and unfractionated heparin. In recent years, new medical and invasive therapies have been developed: anti-platelets (thienopyridines and glycoprotein Ilb/IlIa inhibitors), antithrombins (low molecularweight heparins) and most recently, factor Xa inhibitors (pentasaccharides). As new treatments are rapidly added, clinicians are constantly challenged to incorporate new information and guidelines into their practices in a timely fashion

Cardiac injections of AntagomiRs as a novel tool for knockdown of miRNAs during heart development

Background: Studying microRNA networks during heart development is essential to obtain a better understanding of developmental defects and diseases associated with the heart and to identify novel opportunities for therapeutics. Here we highlight the advantages of chicken embryos as a vertebrate model for studying intermediate processes of heart development. Avians develop a four-chambered heart closely resembling human anatomy and they develop ex utero, which makes them easily accessible. Furthermore, embryos are available all year with a steady supply. Results: In this report we established a novel method for the knockdown of microRNA function by microinjecting AntagomiRs into the chicken heart in ovo. Our approach enables the targeted delivery of antagomirs into a locally restricted area and is not impacted by circulation. After further embryo development the successful miRNA knockdown was confirmed. Loss of function phenotypes can be evaluated rapidly, compared to more time-consuming genetic ablation experiments. The local application avoids potential systemic effects of microRNA knockdown, therefore allowing the assessment of impacts on heart development only. The method can be adjusted for different stages of chicken embryos (HH13-HH18) as well as for knockdown or targeted overexpression of coding genes. Conclusion: In conclusion our method allows targeted and locally restricted delivery of Antagomirs to the heart leading to successful knockdown of microRNA function. This method enables rapid phenotypic assessment, for example by gene expression analysis of multiple cardiac genes

Reproduction in the externally brooding sea anemone Epiactis georgiana in the Antarctic Peninsula and the Weddell Sea

14 pages, 8 figures, 2 tablesExternal parental care is uncommon among actiniarians but common in Epiactis species. Here, several aspects of reproduction are analyzed for of one of them, Epiactis georgiana. Samples were collected in December, January, February, March, and April in the Antarctic Peninsula and the eastern Weddell Sea, during 1998, 2000, 2002, and 2003. Most sexually mature individuals of E. georgiana are male or female, but some are hermaphrodites. This is the first report of hermaphroditism in E. georgiana, which is the third species of the genus with this sexual pattern. The results suggest that oogenesis starts in December and that at least two generations of oocytes overlap; a third generation is often brooded externally. Putative fertilization is likely internal, and larvae and/or embryos are externally brooded on the distal part of the adult column until an advanced developmental stage. Apparently E. georgiana reproduces seasonally, probably releasing the embryos/larvae in the last months of the austral spring (December). Inter-individual variability was observed in gametogenesis. In addition, specimens from the Antarctic Peninsula were larger than those from the Weddell Sea. This study represents the first step in understanding the reproductive mode of E. georgianaSpecial thanks are addressed to Prof. Dr. Wolf Arntz (Alfred-Wegener-Institute, Bremerhaven, Germany) who made possible our participation in several Antarctic projects and cruises. We extend our acknowledgements to the officers and crew of the R/V Polarstern and many colleagues on board during the EASIZ, ANDEEP, and BENDEX cruises for their valuable assistance. Thanks to M. Conradi (Universidad de Sevilla) who collected a considerable amount of the material analyzed in this manuscript. Comments from M. Daly, D. Fautin, and an anonymous reviewer substantially improved this manuscript. Support was provided by a MCT-CSICgrant (I3P-BPD2001-1) to E. Rodríguez and Spanish CICYT projects: ANT97-1533-E, ANT98-1739-E, ANT99-1608-E, REN2001-4269-E/ANT, REN2003-04236, and CGL2004-20141-E. This is a contribution to the SCAR program, Ecology of the Antarctic Sea Ice Zone (EASIZ) and ANDEEP contribution 159Peer reviewe

Squalene stimulates a key innate immune cell to foster wound healing and tissue repair

Anti-inflammatory effects of virgin olive oil (VOO) have been described recently, along with its wound healing effect. One of the main minor compounds found in VOO is squalene (SQ), which also possesses preventive effects against skin damage and anti-inflammatory properties. The inflammatory response is involved in wound healing and manages the whole process by macrophages, among others, as the main innate cells with a critical role in the promotion and resolution of inflammation for tissue repair. Because of that, this work is claimed to describe the role that squalene exerts in the immunomodulation of M1 proinflammatory macrophages, which are the first cells implicate in recent injuries. Pro- and anti-inflammatory cytokines were analysed using TPH1 cell experimental model. SQ induced an increase in the synthesis of anti-inflammatory cytokines, such as IL-10, IL-13, and IL-4, and a decrease in proinflammatory signals, such as TNF-α and NF-κB in M1 proinflammatory macrophages. Furthermore, SQ enhanced remodelling and repairing signals (TIMP-2) and recruitment signals of eosinophils and neutrophils, responsible for phagocytosis processes. These results suggest that SQ is able to promote wound healing by driving macrophage response in inflammation. Therefore, squalene could be useful at the resolution stage of wound healing