Cys-Ph-TAHA: a lanthanide binding tag for RDC and PCS enhanced protein NMR

Here we present Cys-Ph-TAHA, a new nonadentate lanthanide tag for the paramagnetic labelling of proteins. The tag can be easily synthesized and is stereochemically homogenous over a wide range of temperatures, yielding NMR spectra with a single set of peaks. Bound to ubiquitin, it induced large residual dipolar couplings and pseudocontact shifts that could be measured easily and agreed very well with the protein structure. We show that Cys-Ph-TAHA can be used to label large proteins that are biochemically challenging such as the Lac repressor in a 90 kDa ternary complex with DNA and inducer

A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

Meeting abstrac

Onboard Trajectory Generation for Entry Vehicles via Adaptive Multivariate Pseudospectral Interpolation

harvest AIAA 2016-2115Astrodynamics & Space Mission

The efficacy of postoperative radiotherapy in fibromatosis

10th Eurasian Hematology Oncology Congress -- OCT 08-11, 2019 -- Istanbul, TURKEYWOS: 000489290100045

Letter to the Editor - Transient Cerebellar Mutism After Posterior Fossa Surgery

Sir, A 4-year-old boy presented with a two-month history of headache, intermittent nausea and vomiting. Ataxic gait appeared in last week. He had ataxic gait, bilateral dysdiadochokinesia, dysmetria, horizontal nystagmus, and papilloedema. Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) of brain showed a tumour in fourth ventricle and hydrocephalus. Two days after the ventriculo-peritoneal shunt application, suboccipital craniectomy and tumour excision with vermian incision was performed. The patient was operated in the sitting position and there was no peri-operative hypotension. In postoperative period, CT and MRI revealed that the tumour has been removed totally. Three days after the operation, the patient developed mutism, which lasted for three weeks. At the end of the third week the mutism regressed but dysarthric speech continued for two more weeks and then the speech returned to normal. Histopathological diagnosis of tumour was ependymoma. 99mTC hexamethyl-propyleneamine oxime-single photon emission computed tomographic (SPECT) scan revealed left/right perfusion ratio 0.85 (mutism), 0.99 (post-mutism) in frontal region. This ratio was 0.79 (mutism), 1.06 (post-mutism) in occipital region. In addition to these, perfusion defect area was 0.55 cm3 (mutism), 0.001cm3 (post-mutism) in left frontal region. This area was 0.25cm3 (mutism), 0.04cm3 (post-mutism) in thalamus. Perfusion changes were not observed in cerebellum