PTPN22 gene polymorphism in Takayasu's arteritis

Objective. Takayasu's arteritis (TA) is a chronic, rare granulomatous panarteritis of unknown aetiology involving mainly the aorta and its major branches. In this study, genetic susceptibility to TA has been investigated by screening the functional single nucleotide polymorphism (SNP) of PTPN22 gene encoding the lymphoid-specific protein tyrosine phosphatase. Methods. Totally, 181 patients with TA and 177 healthy controls are genotyped by PCR-RFLP method for the SNP rs2476601 (A/G) of PTPN22 gene. Polymorphic region was amplified by PCR and digested with Xcm I enzyme. Results. Detected frequencies of heterozygous genotype (AG) were 5.1% (9/177) in control group and 3.8% (7/181) in TA group (P = 0.61, odds ratio: 0.75, 95% CI: 0.3, 2.0). No association with angiographic type, vascular involvement or prognosis of TA was observed either. Conclusion. The distribution of PTPN22 polymorphism did not reveal any association with TA in Turkey. © The Author 2008. Published by Oxford University Press on behalf of the British Society for Rheumatology. All rights reserved

Effects of different raising systems on colour and quality characteristics of Turkish Pekin duck meats

The current trial was conducted to determine the influence of different raising systems on the meat quality properties of male Turkish Pekin ducks. Ninety male ducklings were randomly allocated to three experimental groups: an animal-fish integrated farming group (IG), a non-animal-fish integrated farming group (NIG) and a poultry house group (PHG). All ducklings were fed a starter diet from weeks 2 to 6 and a finisher diet from weeks 6 to 10. Feed and water were offered ad libitum. At the end of the trial all ducks were slaughtered and the carcasses were stored at 3 °C for 24 hours, after which L*, a* and b* values of the carcass skins were measured. After standard dissection of carcasses, pectoralis muscles were obtained on which pH, colour (L*, a*, b*, C and H), total aerobic mesophilic, total aerobic psychrotrophic, lactic acid bacteria, Micrococcus/Staphylococcus, yeast-mould and Enterobacteriaceae counts were determined. The different raising systems of the ducks had significant effects on the pH, total aerobic mesophilic, Enterobacteriaceae, and L* and b* values of the pectoralis muscle. The lowest pH, total aerobic mesophilic and Enterobacteriaceae counts were found in the PHG group. The lowest L* values for the pectoralis muscle were found in the IG group while the highest a* value was recorded in the IG group. Significant differences in skin colour were observed between the experimental groups. For all production groups, all microbial counts were found to be within acceptable ranges. However, pH, total aerobic mesophilic and Enterobacteriaceae results were found to be lower in the PHG group than in the other groups. Different raising systems were thus found to affect the meat and skin colour of ducks, which may influence the preference of consumers. Keywords: Pekin duck, integrated farming, carcass and meat colour, microbial propertiesSouth African Journal of Animal Science Vol. 38 (3) 2008: pp. 217-22

Balancing of U-type assembly systems using simulated annealing

The paper presents a new simulated annealing (SA)-based algorithm for the assembly line-balancing problem with a U-type configuration. The proposed algorithm employs an intelligent mechanism to search a large solution space. U-type assembly systems are becoming increasingly popular in today's modern production environments since they are more general than the traditional assembly systems. In these systems, tasks are to be allocated into stations by moving forward and backward through the precedence diagram in contrast to a typical forward move in the traditional assembly systems. The performance of the algorithm is measured by solving a large number of benchmark problems available in the literature. The results of the computational experiments indicate that the proposed SA-based algorithm performs quite effectively. It also yields the optimal solution for most problem instances. Future research directions and a comprehensive bibliography are also provided here

Derivation of Del180 from sediment core log data\u27 Implications for millennial-scale climate change in the Labrador Sea

Sediment core logs from six sediment cores in the Labrador Sea show millennial-scale climate variability during the last glacial by recording all Heinrich events and several major Dansgaard-Oeschger cycles. The same millennial-scale climate change is documented for surface water δ18O records of Neogloboquadrina pachyderma (left coiled); hence the surface water δ18O record can be derived from sediment core logging by means of multiple linear regression, providing a paleoclimate proxy record at very high temporal resolution (70 years). For the Labrador Sea, sediment core logs contain important information about deepwater current velocities and also reflect the variable input of ice-rafted debris from different sources as inferred from grain-size analysis, the relation of density and P wave velocity, and magnetic susceptibility. For the last glacial, faster deepwater currents, which correspond to highs in sediment physical properties, occurred during iceberg discharge and lasted from several centuries to a few millennia. Those enhanced currents might have contributed to increased production of intermediate waters during times of reduced production of North Atlantic Deep Water. Hudson Strait might have acted as a major supplier of detrital carbonate only during lowered sea level (greater ice extent). During coldest atmospheric temperatures over Greenland, deepwater currents increased during iceberg discharge in the Labrador Sea, then surface water freshened shortly thereafter, while the abrupt atmospheric temperature rise happened after a larger time lag of ≥ 1 kyr. The correlation implies a strong link and common forcing for atmosphere, sea surface, and deep water during the last glacial at millennial timescales but decoupling at orbital timescales

The role of explicit contrast in adjective acquisition: a cross-linguistic longitudinal study of adjective production in spontaneous child speech and parental input

Experimental studies demonstrate that contrast helps toddlers to extend the meanings of novel adjectives. This study explores whether antonym co-occurrence in spontaneous speech also has an effect on adjective use by the child. The authors studied adjective production in longitudinal speech samples from 16 children (16–36 months) acquiring eight different languages. Adjectives in child speech and child-directed speech were coded as either unrelated or related to a contrastive term in the preceding context. Results show large differences between children in the growth of adjective production. These differences are strongly related to contrast use. High contrast users not only increase adjective use earlier, but also reach a stable level of adjective production in the investigated period. Average or low contrast users increase their adjective production more slowly and do not reach a plateau in the period covered by this study. Initially there is a strong relation between contrast use in child speech and child-directed speech, but this relation diminishes with age. </jats:p

A novel approach for preventing esophageal stricture formation: olmesartan prevented apoptosis

Accidentally ingested corrosive substances can cause functional and structural damage to the esophageal tissue resulting in stricture formation. It has been reported that the administration of olmesartan (OLM) can have anti-inflammatory, antifibrotic and antiapoptotic effects on injured tissue. The aim of our study was to check if OLM could prevent formation of scars in the corrosive esophageal burn model. Fifty-one Wistar Albino rats were divided into six groups: Control, Sham, OLM, Sham + OLM, Burn, and Burn + OLM. Olmesartan (5 mg/kg) was given by gavage once per day for 21 consecutive days after injury. The morphology of the esophagus was assessed after Masson trichrome staining, and apoptosis was evaluated using the terminal deoxynucleotidyl transferased UTP nick end labeling (TUNEL) method. The serum nucleosomes (as an indicator of apoptosis), serum p53 protein, and esophageal tissue p53 protein levels of each group were measured by immunoassays. Muscularis mucosa damage, submucosal collagen deposition, and tunica muscularis injury in the Burn + OLM group decreased significantly compared with the Burn group (p &lt; 0.05). Similarly, the number of apoptotic cells in the Burn + OLM group decreased compared with the Burn group (p &lt; 0.05). Serum levels of nucleosomes and p53 and tissue of p53 protein did not differ between the groups. Exogenously administered OLM can effectively prevent the occurrence of esophageal strictures caused by corrosive esophageal burns. (Folia Histochemica et Cytobiologica 2014, Vol. 52, No. 1, 29–35

Divergent in situ expression of IL-31 and IL-31RA between bullous pemphigoid and pemphigus vulgaris

Bullous pemphigoid (BP) and pemphigus vulgaris (PV) are two major autoimmune blistering skin diseases. Unlike PV, BP is accompanied by intense pruritus, suggesting possible involvement of the pruritogenic cytokine IL-31. However, the underlying mechanisms of the clinical difference between BP and PV in terms of pruritus are not fully understood. To compare the expression levels of IL-31 and its receptor IL-31RA in the lesional skin, including peripheral nerves in BP and PV patients, immunohistochemical staining for IL-31 and IL-31RA was performed in skin samples of BP and PV patients and healthy controls (HC). The IL-31RA-expressing area in epidermis and peripheral nerves was analysed using ImageJ and the percentage of positive cells for IL-31/IL-31RA in dermal infiltrating cells was manually quantified. Quantitative analyses revealed that IL-31/IL-31RA expressions in the epidermis and dermal infiltrate were significantly increased in BP compared to PV and HC. The difference between BP and PV became more obvious when advanced bullous lesions were compared. Peripheral nerves in BP lesions presented significantly higher IL-31RA expression compared to PV lesions. In conclusion, we found significantly augmented expressions of IL-31/IL-31RA in BP lesions, including peripheral nerves, in comparison to PV. These results suggest a possible contribution of IL-31/IL-31RA signalling to the difference between BP and PV in the facilitation of pruritus and local skin inflammation, raising the possibility of therapeutic targeting of the IL-31/IL-31RA pathway in BP patients