DNA Rereplication Is Susceptible to Nucleotide-Level Mutagenesis.

The sources of genome instability, a hallmark of cancer, remain incompletely understood. One potential source is DNA rereplication, which arises when the mechanisms that prevent the reinitiation of replication origins within a single cell cycle are compromised. Using the budding yeast Saccharomyces cerevisiae, we previously showed that DNA rereplication is extremely potent at inducing gross chromosomal alterations and that this arises in part because of the susceptibility of rereplication forks to break. Here, we examine the ability of DNA rereplication to induce nucleotide-level mutations. During normal replication these mutations are restricted by three overlapping error-avoidance mechanisms: the nucleotide selectivity of replicative polymerases, their proofreading activity, and mismatch repair. Using lys2InsEA14 , a frameshift reporter that is poorly proofread, we show that rereplication induces up to a 30× higher rate of frameshift mutations and that this mutagenesis is due to passage of the rereplication fork, not secondary to rereplication fork breakage. Rereplication can also induce comparable rates of frameshift and base-substitution mutations in a more general mutagenesis reporter CAN1, when the proofreading activity of DNA polymerase ε is inactivated. Finally, we show that the rereplication-induced mutagenesis of both lys2InsEA14 and CAN1 disappears in the absence of mismatch repair. These results suggest that mismatch repair is attenuated during rereplication, although at most sequences DNA polymerase proofreading provides enough error correction to mitigate the mutagenic consequences. Thus, rereplication can facilitate nucleotide-level mutagenesis in addition to inducing gross chromosomal alterations, broadening its potential role in genome instability

REALIZATION OF BROADBAND AND INDEPENDENT POLARIZATION METAMATERIAL PERFECT ABSORBER BASED ON THE THIRD-ORDER RESONANCE

In this report, we studied numerically and experimentally the electromagnetic properties of perfect MAs using ring-shaped structures at microwave frequencies. By creating a magnetic resonance, the ring structure confines electromagnetic energy at the first- and third-order resonances. And then, we leveraged the super-cell structures, which consist of different rings in one unit cell to obtain broadband absorption. The results showed that the absorption band of MA is broaden from 1.3 to 2.17 GHz when the number of rings in the unit cell increases from four to nine-ring

The Impact of TikTok UGC Videos on Online Purchase Intention: Mediating Role of Cognitive States

Background: User-Generated Content (UGC) videos have received considerable attention in recent years thanks to their great potential for buyers and sellers. However, the effect of this content on consumer behavior remains unclear, especially in the context of developing countries (e.g., Vietnam). By applying the Stimulus-Organism-Response model (S-O-R model), this paper examines the effect of UGC videos on online purchase intention. Moreover, the mediating role of cognitive responses, consisting of perceived credibility, perceived diagnosticity, and mental imagery, is also examined to offer valuable insights to businesses, enabling them to leverage and effectively promote the trends of UGC videos. Method: A convenience sampling method was employed to collect the data. A total of 318 valid respondents participated in this survey. The data was analyzed with the Partial Least Squares Structural Equation Modeling method (PLS-SEM). Results: The findings show that UGC videos have a direct impact on online purchase intention. This paper also verified that cognitive states mediate the relationship between stimuli and subsequent behavioral intentions. Conclusion: Our research findings enrich the literature on consumer’s online purchase intentions by applying the S-O-R framework by highlighting the role of cognitive responses, and improving generalizability by contributing additional consumer behavior findings in developing Asian nations. Moreover, this paper offers businesses insights into the formation of customers\u27 purchase intentions while watching UGC videos. Based on that, this paper raises practical recommendations regarding promoting the UGC video trend and creating UGC videos effectively to improve the cognitive states perceived by customers, including credibility, diagnostic value, and mental imagery

THE DIFFICULTIES IN ORAL PRESENTATION OF ENGLISH-MAJORED JUNIORS AT TAY DO UNIVERSITY, VIETNAM

It could be broadly accepted that oral presentations are becoming important for students. It is required in almost every field and in the university environment. To succeed in the university environment and in their future jobs, these students need to improve their oral presentation skills. However, one of the drawbacks of using oral presentations in the language classroom is that students often find oral presentations extremely challenging. Therefore, the researcher conducted this research with the main goal of finding out common problems when giving an oral presentation to English-majored juniors at Tay Do University. Ninety juniors majoring in English at Tay Do University were selected to take part in the study. Data are gathered through questionnaires and interviews. The researcher used quantitative and qualitative methods to do the research. From the collected data, when they give a presentation, students usually make some mistakes such as problems in vocabulary, grammar, pronunciation, psychological and background knowledge. Through this study, they would recognize their own problems when giving a presentation. Besides, understanding students' learning difficulties may also enable teachers to help students develop effective learning strategies and ultimately improve their presentation skills. It is hoped that this research can be helpful for not only students but also teachers in learning and teaching English.  Article visualizations

Impact of trained human resources, adoption of technology and international standards on the improvement of accounting and auditing activities in the agricultural sector in Viet Nam

Recently, the adoption of accounting and auditing standards in the agricultural sector has been a global phenomenon that has gained increasing trend due to the significant role of the agricultural industry in the country's economy. Thus, the present study examines the impact of trained human resources, technology adoption, and international standards on improving accounting and auditing activities in the agricultural sector in Vietnam. The present research investigates the moderating impact of agricultural, institutional support among the linkage of trained human resources, technology adoption, international standards, and improvement of accounting and auditing activities in the agricultural sector. The present article has adopted the questionnaires to gather the primary data from selected respondents. The current research has applied the smart-PLS to test the study's hypotheses. The results revealed that trained human resources, technology adoption, and international standards positively impact the improvement of accounting and auditing activities in the agricultural sector. The findings also indicated that the agricultural institutional support significantly moderates the linkage of trained human resources, technology adoption, international standards, and improved accounting and auditing activities in the agricultural sector.Nguyen Van Hoa (University of Kinh Bac (UKB)), Nguyen Thi Hanh Duyen (Vinh University), Vu Ngoc Huyen (Viet Nam National University of Agriculture (VNUA)), Hoang Vu Quang (Researcher at Institute of Policy and Strategy for Agriculture and Rural Development (IPSARD)), Nguyen Van Huong (Hung Yen University of Technology and Education (UTEHY)), Nguyen Thi Cam Tu (National Economics University (NEU)), Bui Thi Minh Nguyet (Vietnam National University of Forestry (VNUF))Includes bibliographical references

Primary pulmonary T-cell lymphoma mimicking asthma and community-acquired pneumonia: a rare case report

Primary pulmonary T-cell lymphoma (PPTL) is a rare disease. Diagnosing PPTL is challenging due to non-specific clinical symptoms and imaging. A 32-year-old female presented with persistent fever, cough, and dyspnoea. The symptoms were initially treated as asthma and community-acquired pneumonia without improvement. Chest computed tomography (CT) revealed bilateral consolidations with a CT angiogram sign, and flexible bronchoscopy showed infiltrative lesions causing bronchial stenosis. Histopathological examination of the tissue biopsy identified T-cell lymphoma through immunohistochemical staining positive for CD3. This case highlights the importance of considering differential diagnoses such as PPTL in patients with atypical presentations of asthma or non-resolving pneumonia. This case also demonstrates the diagnostic utility of flexible bronchoscopy in identifying airway obstruction due to malignant cells, which can mimic asthma

Salinity Gradient Energy from Expansion and Contraction of Poly(allylamine hydrochloride) Hydrogels

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A Novel Wideband Circularly Polarized Antenna for RF Energy Harvesting in Wireless Sensor Nodes

A novel wideband circularly polarized antenna array using sequential rotation feeding network is presented in this paper. The proposed antenna array has a relative bandwidth of 38.7% at frequencies from 5.05 GHz to 7.45 GHz with a highest gain of 12 dBi at 6 GHz. A corresponding left-handed metamaterial is designed in order to increase antenna gain without significantly affecting its polarization characteristics. The wideband circularly polarized antenna with 2.4 GHz of bandwidth is a promising solution for wireless communication system such as tracking or wireless energy harvesting from Wi-Fi signal based on IEEE 802.11ac standard or future 5G cellular. A potential application of this antenna as a receiving antenna for RF-DC device to obtain DC power for a wireless sensor node from Wi-Fi signal is shown

Klebsiella pneumoniae Multiresistance Plasmid pMET1: Similarity with the Yersinia pestis Plasmid pCRY and Integrative Conjugative Elements

Dissemination of antimicrobial resistance genes has become an important public health and biodefense threat. Plasmids are important contributors to the rapid acquisition of antibiotic resistance by pathogenic bacteria.The nucleotide sequence of the Klebsiella pneumoniae multiresistance plasmid pMET1 comprises 41,723 bp and includes Tn1331.2, a transposon that carries the bla(TEM-1) gene and a perfect duplication of a 3-kbp region including the aac(6')-Ib, aadA1, and bla(OXA-9) genes. The replication region of pMET1 has been identified. Replication is independent of DNA polymerase I, and the replication region is highly related to that of the cryptic Yersinia pestis 91001 plasmid pCRY. The potential partition region has the general organization known as the parFG locus. The self-transmissible pMET1 plasmid includes a type IV secretion system consisting of proteins that make up the mating pair formation complex (Mpf) and the DNA transfer (Dtr) system. The Mpf is highly related to those in the plasmid pCRY, the mobilizable high-pathogenicity island from E. coli ECOR31 (HPI(ECOR31)), which has been proposed to be an integrative conjugative element (ICE) progenitor of high-pathogenicity islands in other Enterobacteriaceae including Yersinia species, and ICE(Kp1), an ICE found in a K. pneumoniae strain causing primary liver abscess. The Dtr MobB and MobC proteins are highly related to those of pCRY, but the endonuclease is related to that of plasmid pK245 and has no significant homology with the protein of similar function in pCRY. The region upstream of mobB includes the putative oriT and shares 90% identity with the same region in the HPI(ECOR31).The comparative analyses of pMET1 with pCRY, HPI(ECOR31), and ICE(Kp1 )show a very active rate of genetic exchanges between Enterobacteriaceae including Yersinia species, which represents a high public health and biodefense threat due to transfer of multiple resistance genes to pathogenic Yersinia strains

Control of Flowering and Cell Fate by LIF2, an RNA Binding Partner of the Polycomb Complex Component LHP1

Polycomb Repressive Complexes (PRC) modulate the epigenetic status of key cell fate and developmental regulators in eukaryotes. The chromo domain protein LIKE HETEROCHROMATIN PROTEIN1 (LHP1) is a subunit of a plant PRC1-like complex in Arabidopsis thaliana and recognizes histone H3 lysine 27 trimethylation, a silencing epigenetic mark deposited by the PRC2 complex. We have identified and studied an LHP1-Interacting Factor2 (LIF2). LIF2 protein has RNA recognition motifs and belongs to the large hnRNP protein family, which is involved in RNA processing. LIF2 interacts in vivo, in the cell nucleus, with the LHP1 chromo shadow domain. Expression of LIF2 was detected predominantly in vascular and meristematic tissues. Loss-of-function of LIF2 modifies flowering time, floral developmental homeostasis and gynoecium growth determination. lif2 ovaries have indeterminate growth and produce ectopic inflorescences with severely affected flowers showing proliferation of ectopic stigmatic papillae and ovules in short-day conditions. To look at how LIF2 acts relative to LHP1, we conducted transcriptome analyses in lif2 and lhp1 and identified a common set of deregulated genes, which showed significant enrichment in stress-response genes. By comparing expression of LHP1 targets in lif2, lhp1 and lif2 lhp1 mutants we showed that LIF2 can either antagonize or act with LHP1. Interestingly, repression of the FLC floral transcriptional regulator in lif2 mutant is accompanied by an increase in H3K27 trimethylation at the locus, without any change in LHP1 binding, suggesting that LHP1 is targeted independently from LIF2 and that LHP1 binding does not strictly correlate with gene expression. LIF2, involved in cell identity and cell fate decision, may modulate the activity of LHP1 at specific loci, during specific developmental windows or in response to environmental cues that control cell fate determination. These results highlight a novel link between plant RNA processing and Polycomb regulation