Quantification of three macrolide antibiotics in pharmaceutical lots by HPLC: Development, validation and application to a simultaneous separation

A new validated high performance liquid chromatographic (HPLC) method with rapid analysis time and high efficiency, for the analysis of erythromycin, azithromycin and spiramycin, under isocratic conditions with ODB RP18 as a stationary phase is described. Using an eluent composed of acetonitrile –2-methyl-2-propanol –hydrogenphosphate buffer, pH 6.5, with 1.5% triethylamine (33:7: up to 100, v/v/v), delivered at a flow-rate of 1.0 mL min-1. Ultra Violet (UV) detection is performed at 210 nm. The selectivity is satisfactory enough and no problematic interfering peaks are observed. The procedure is quantitatively characterized and repeatability, linearity, detection and quantification limits are very satisfactory. The method is applied successfully for the assay of the studied drugs in pharmaceutical dosage forms as tablets and powder for oral suspension. Recovery experiments revealed recovery of 97.13–100.28%

Long baseline interferometry in the visible: the FRIEND project

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Asymmetric shocks in χ\chi Cyg observed with linear spectropolarimetry

Aims: From a coherent interpretation of the linear polarisation detected in the spectral lines of the Mira star $\chi$ Cyg, we derive information about the dynamics of the stellar photosphere, including pulsation. Methods: From spectropolarimetric observations of $\chi$ Cyg, we perform careful analysis of the polarisation signals observed in atomic and molecular lines, both in absorption and emission, using the radiative transfer in the polarisation context, through two mechanisms: intrinsic polarisation and continuum depolarisation. We also explain the observed line doubling phenomenon in terms of an expanding shell in spherical geometry, which allows us to pinpoint the coordinates over the stellar disk with enhanced polarisation. Results: We find that the polarised spectrum of $\chi$ Cyg is dominated by intrinsic polarisation, with a negligible continuum depolarisation. The observed polarised signals can only be explained by assuming that this polarisation is locally enhanced by velocity fields. During the pulsation, radial velocities are not homogeneous over the disk. We map these regions of enhanced velocities. Conclusions: We have set an algorithm to distinguish in any stellar spectra of linear polarisation the origin of this polarisation and the way to increase signal by coherently adding many lines with an appropriated weight. Applied to the Mira star $\chi$ Cyg, we reached the unexpected result that during the pulsation, velocities are radial but not homogeneous over the disk. The reason for these local velocity enhancements are probably related to the interplay between the atmospheric pulsation dynamics and the underlying stellar convection