Treadmill running restores MDMA-mediated hyperthermia prevented by inhibition of the dorsomedial hypothalamus

The contribution of exercise to hyperthermia mediated by MDMA is not known. We recently showed that inhibiting the dorsomedial hypothalamus (DMH) attenuated spontaneous locomotion and hyperthermia and prevented deaths in rats given MDMA in a warm environment. The goal of this study was to confirm that restoring locomotion through a treadmill would reverse these effects thereby confirming that locomotion mediated by the DMH contributes to MDMA-mediated hyperthermia. Rats were randomized to receive bilateral microinjections, into the region of the DMH, of muscimol (80pmol/100nl) or artificial CSF followed by a systemic dose of either MDMA (7.5mg/kg, i.v.) or saline. Immediately after the systemic injection, rats were placed on a motorized treadmill maintained at 32°C. Rats were exercised at a fixed speed (10m/min) until their core temperature reached 41°C. Our results showed that a fixed exercise load abolished the decreases in temperature and mortality, seen previously with inhibition of the DMH in freely moving rats. Therefore, locomotion mediated by neurons in the DMH is critical to the development of hyperthermia from MDMA

Inhibition of the dorsomedial hypothalamus, but not the medullary raphe pallidus, decreases hyperthermia and mortality from MDMA given in a warm environment.

The central mechanisms through which MDMA mediates life-threatening hyperthermia when taken in a warm environment are not well described. It is assumed that MDMA alters normal thermoregulatory circuits resulting in increased heat production through interscapular brown adipose tissue (iBAT) and decreased heat dissipation through cutaneous vasoconstriction. We studied the role of the dorsomedial hypothalamus (DMH) and medullary raphe pallidus (mRPa) in mediating iBAT, tail blood flow, and locomotor effects produced by MDMA. Rats were instrumented with guide cannulas targeting either the DMH or the mRPa-brain regions involved in regulating iBAT and cutaneous vascular beds. In all animals, core temperature and locomotion were recorded with surgically implanted telemetric transmitters; and additionally either iBAT temperature (via telemetric transmitter) or tail artery blood flow (via tail artery Doppler cuff) were also recorded. Animals were placed in an environmental chamber at 32°C and microinjected with either control or the GABA agonist muscimol (80pmol) followed by an intravenous injection of saline or MDMA (7.5 mg kg-1). To prevent undue suffering, a core temperature of 41°C was chosen as the surrogate marker of mortality. Inhibition of the DMH, but not the mRPa, prevented mortality and attenuated hyperthermia and locomotion. Inhibition of either the DMH or the mRPa did not affect iBAT temperature increases or tail blood flow decreases. While MDMA increases iBAT thermogenesis and decreases heat dissipation through cutaneous vasoconstriction, thermoregulatory brain regions known to mediate these effects are not involved. Rather, the finding that inhibiting the DMH decreases both locomotion and body temperature suggests that locomotion may be a key central contributor to MDMA-evoked hyperthermia

Long-term X-ray changes in the emission from the anomalous X-ray pulsar 4U 0142+61

We present results obtained from X-ray observations of the anomalous X-ray pulsar (AXP) 4U 0142+61 taken between 2000-2007 using XMM-Newton, Chandra and Swift. In observations taken before 2006, the pulse profile is observed to become more sinusoidal and the pulsed fraction increased with time. These results confirm those derived using the Rossi X-ray Timing Explorer and expand the observed evolution to energies below 2 keV. The XMM-Newton total flux in the 0.5-10 keV band is observed to be nearly constant in observations taken before 2006, while an increase of ~10% is seen afterwards and coincides with the burst activity detected from the source in 2006-2007. After these bursts, the evolution towards more sinusoidal pulse profiles ceased while the pulsed fraction showed a further increase. No evidence for large-scale, long-term changes in the emission as a result of the bursts is seen. The data also suggest a correlation between the flux and hardness of the spectrum, with brighter observations on average having a harder spectrum. As pointed out by other authors, we find that the standard blackbody plus power-law model does not provide the best spectral fit to the emission from 4U 0142+61. We also report on observations taken with the Gemini telescope after two bursts. These observations show source magnitudes consistent with previous measurements. Our results demonstrate the wide range of X-ray variability characteristics seen in AXPs and we discuss them in light of current emission models for these sources.Comment: 10 pages, 9 figures, in emulateapj style. Submitted to Ap

Impact of the lateral blast on the spatial pattern and grain size characteristics of the 18 May 1980 Mount St. Helens fallout deposit

International audienceThe 18 May 1980 eruption of Mount St. Helens started with a lateral blast that fed a pyroclastic surge, which then uplifted to form a co-blast plume. Thirty minutes later, Plinian activity started at the vent and fluctuated in intensity for ~9 h. The resulting fallout deposit, documented to > 600 km from vent, presents some striking features: (1) displacement of the overall deposit to the north of the vent, (2) a secondary thickness and mass maximum at ~300 km from vent, (3) a total grain size distribution dominated by fine ash (62 wt % of the deposit < 63 μm), and (4) individual grain size distributions that vary dramatically in the crosswind direction from strongly bimodal in the south to skewed unimodal in the north. Results from a new deconvolution of the individual grain size distributions show that they are a combination of a coarse subpopulation that decreases in size with distance from vent and a constant fine subpopulation with a mean of ∼15 μm. Relative proportions of each subpopulation vary asymmetrically in the crosswind directions, with the fine subpopulation preponderant toward the north and the coarse one dominating the south of the deposit, both reach their absolute maxima in mass on the deposit axis. Componentry analyses of selected samples show that blast-derived material is greatly enriched toward the north of the deposit. These results indicate that the co-blast plume dispersed fine-grained material over great distances and dominated the fine subpopulation. Comparison with reanalysis data of atmospheric wind fields and satellite images of the spreading ash cloud suggests contrasting ash transport and depositional processes for the (early) co-blast plume and the (later) vent-derived Plinian plumes. The co-blast plume is displaced to the north; it had a high overshoot height, and eastward dispersion via strong winds low in the stratosphere (~10-15 km). The Plinian plumes were lower and dispersed most of the material to the southeast as the direction of high-velocity winds shifted just before the late climactic Plinian eruptive phase. Fine ash (fine subpopulation) was deposited continuously throughout the deposit, with an increase of sedimentation rate ~300 km from the vent where there is a secondary maximum in the deposit mass and thickness. Fine ash probably settled by a combination of enhanced sedimentation mechanisms, including not only aggregation but also gravitational convective instabilities of cloud base, hydrometeor formation and destruction, and entrainment of small particles by larger ones. Finally, we show that half of the deposit (by mass) in the medial area was deposited by the co-blast plume, and that a significant proportion of the Mount St. Helens fallout deposit is nonjuvenile, which has implications for the magmatic budget of this eruption

Multi-wavelength variability of the magnetar 4U 0142+61

We have collected data spanning seven years of observations of the magnetar 4U 0142+61 in the infrared, optical and soft X-rays. These combine our own observations and analysis of archival data. We find that the source is variable in the optical, in contrast to what had been previously reported, that the K-band flux can vary by over a magnitude on the time-scale of days, and that the X-ray pulsed flux is not obviously correlated with either the total X-ray flux or infrared and optical fluxes. Furthermore, from multi-color photometry of the source within single nights, we conclude that there are two separate components to the infrared emission. The overall picture is unclear, and prompts the need for further, more frequent observations.Comment: 20 pages, published in Ap

The first multi-wavelength campaign of AXP 4U 0142+61 from radio to hard X-rays

For the first time a quasi-simultaneous multi-wavelength campaign has been performed on an Anomalous X-ray Pulsar from the radio to the hard X-ray band. 4U 0142+61 was an INTEGRAL target for 1 Ms in July 2005. During these observations it was also observed in the X-ray band with Swift and RXTE, in the optical and NIR with Gemini North and in the radio with the WSRT. In this paper we present the source-energy distribution. The spectral results obtained in the individual wave bands do not connect smoothly; apparently components of different origin contribute to the total spectrum. Remarkable is that the INTEGRAL hard X-ray spectrum (power-law index 0.79 +/- 0.10) is now measured up to an energy of ~230 keV with no indication of a spectral break. Extrapolation of the INTEGRAL power-law spectrum to lower energies passes orders of magnitude underneath the NIR and optical fluxes, as well as the low ~30 microJy (2 sigma) upper limit in the radio band.Comment: 6 pages, 1 figure. To be published in the proceedings of the conference "Isolated Neutron Stars: from the Interior to the Surface" (April 24-28, 2006, London, UK), eds. S. Zane, R. Turolla and D. Pag

Independent of 5-HT1A receptors, neurons in the paraventricular hypothalamus mediate ACTH responses from MDMA

Acute and chronic complications from the substituted amphetamine 3,4-methylenedioxymethamphetamine (MDMA) are linked to activation of the hypothalamic-pituitary-adrenal (HPA) axis. How MDMA activates the HPA axis is not known. HPA responses to stress are known to be mediated through the paraventricular (PVH) hypothalamus and to involve serotonin-1a (5-HT1A) receptors. We sought to determine if the PVH and 5-HT1A receptors were also involved in mediating HPA responses to MDMA. Rats were pretreated with either saline or a 5-HT1A antagonist, WAY-100635 (WAY), followed by a systemic dose of MDMA (7.5mg/kg i.v.). Animals pretreated with WAY had significantly lower plasma ACTH concentrations after MDMA. To determine if neurons in the PVH were involved, and if their involvement was mediated by 5-HT1A receptors, rats implanted with guide cannulas targeting the PVH were microinjected with the GABAA receptor agonist muscimol, aCSF, or WAY followed by MDMA. Compared to aCSF, microinjections of muscimol significantly attenuated the MDMA-induced rise in plasma ACTH (126 vs. 588pg/ml, P=<0.01). WAY had no effect. Our data demonstrates that neurons in the PVH, independent of 5-HT1A receptors, mediate ACTH responses to MDMA

Disinhibiting neurons in the dorsomedial hypothalamus delays the onset of exertional fatigue and exhaustion in rats exercising in a warm environment

Stimulants cause hyperthermia, in part, by increasing heat generation through exercise. Stimulants also delay the onset of fatigue and exhaustion allowing animals to exercise longer. If used in a warm environment, this combination (increased exercise and decreased fatigue) can cause heat stroke. The dorsomedial hypothalamus (DMH) is involved in mediating locomotion from stimulants. Furthermore, inhibiting the DMH decreases locomotion and prevents hyperthermia in rats given stimulants in a warm environment. Whether the DMH is involved in mediating exercise-induced fatigue and exhaustion is not known. We hypothesized that disinhibiting neurons in the dorsomedial hypothalamus (DMH) would delay the onset of fatigue and exhaustion in animals exercising in a warm environment. To test this hypothesis, we used automated video tracking software to measure fatigue and exhaustion. In rats, using wearable mini-pumps, we demonstrated that disinhibiting the DMH, via bicuculline perfusion (5 µM), increased the duration of exercise in a warm environment as compared to control animals (25 ± 3 min vs 15 ± 2 min). Bicuculline-perfused animals also had higher temperatures at exhaustion (41.4 ± 0.2 °C vs 40.0 ± 0.4 °C). Disinhibiting neurons in the DMH also increased the time to fatigue. Our data show that the same region of the hypothalamus that is involved in mediating locomotion to stimulants, is also involved in controlling exhaustion and fatigue. These findings have implications for understanding the cause and treatment of stimulant-induced-hyperthermia

Correlated Infrared and X-ray Flux Changes Following the 2002 June Outburst of the Anomalous X-ray Pulsar 1E 2259+586

We present the results of a near-infrared monitoring program of the Anomalous X-ray Pulsar 1E 2259+586, performed at the Gemini Observatory. This program began three days after the pulsar's 2002 June outburst, and spans ~1.5 years. We find that after an initial increase associated with the outburst, the near-infrared flux decreased continually and reached the pre-burst quiescent level after about one year. We compare both the near-infrared flux enhancement and its decay to those of the X-ray afterglow, and find them to be remarkably consistent. Fitting simple power laws to the RXTE pulsed flux and near-infrared data for t>1 day post-burst, we find the following decay indices: alpha=-0.21+/-0.01 (X-ray), alpha=-0.21+/-0.02 (near-infrared), where flux is a function of time such that F is proportional to t^alpha. This suggests that the enhanced infrared and X-ray fluxes have a physical link post-outburst, most likely from the neutron-star magnetosphere.Comment: 11 pages, 1 figure, accepted for publication in ApJL; minor wording changes, added observation program IDs, improved figure resolutio

Diets of the Barents Sea cod (Gadus morhua) from the 1930s to 2018

A new dataset on the diet of Atlantic cod in the Barents Sea from the 1930s to the present day has been compiled to produce one of the largest fish diet datasets available globally. Atlantic cod is one of the most ecologically and commercially important fish species in the North Atlantic. The stock in the Barents Sea is by far the largest, as a result of both successful management and favourable environmental conditions since the early 2000s. As a top predator, cod plays a key role in the Barents Sea ecosystem. The species has a broad diet consisting mainly of crustaceans and teleost fish, and both the amount and type of prey vary in space and time. The data – from Russia, Norway and the United Kingdom – represent quantitative stomach content records from more than 400 000 fish and qualitative data from 2.5 million fish. Many of the data are from joint collaborative surveys between Norway and Russia. The sampling was conducted throughout each year, allowing for seasonal, annual and decadal comparisons to be made. Visual analysis shows cod diets have changed considerably from the start of the dataset in the 1930s to the present day. There was a large proportion of herring in the diets in the 1930s, whereas in more recent decades capelin, invertebrates and other fish dominate. There are also significant interannual asynchronous fluctuations in prey, particularly capelin and euphausiids. Combining these datasets can help us understand how the environment and ecosystems are responding to climatic changes, and what influences the diet and prey switching of cod. Trends in temperature and variability indices can be tested against the occurrence of different prey items, and the effects of fishing pressure on cod and prey stocks on diet composition could be investigated. The dataset will also enable us to improve parametrization of food web models and to forecast how Barents Sea fisheries may respond in the future to management and to climate change. The Russian data are available through joint projects with the Polar Branch of the Russian Federal Research Institute of Fisheries and Oceanography (VNIRO).publishedVersio