1,132 research outputs found
Molecular architecture of softwood revealed by solid-state NMR
Economically important softwood from conifers is mainly composed of the polysaccharides cellulose, galactoglucomannan and xylan, and the phenolic polymer, lignin. The interactions between these polymers lead to wood mechanical strength and must be overcome in biorefining. Here, we use 13C multidimensional solid-state NMR to analyse the polymer interactions in never-dried cell walls of the softwood, spruce. In contrast to some earlier softwood cell wall models, most of the xylan binds to cellulose in the two-fold screw conformation. Moreover, galactoglucomannan alters its conformation by intimately binding to the surface of cellulose microfibrils in a semi-crystalline fashion. Some galactoglucomannan and xylan bind to the same cellulose microfibrils, and lignin is associated with both of these cellulose-bound polysaccharides. We propose a model of softwood molecular architecture which explains the origin of the different cellulose environments observed in the NMR experiments. Our model will assist strategies for improving wood usage in a sustainable bioeconomy
Recommended from our members
Covalent interactions between lignin and hemicelluloses in plant secondary cell walls.
The plant secondary cell wall is a complex structure composed of polysaccharides and lignin, and is a key evolutionary innovation of vascular land plants. Although cell wall composition is well understood, the cross-linking of the different polymers is only now yielding to investigation. Cross-linking between hemicelluloses and lignin occurs via two different mechanisms: incorporation into lignin by radical coupling of ferulate substitutions on xylan in commelinid monocots, and incorporation of hemicellulosic glycosyl residues by re-aromatisation of lignification intermediates. Recent genetic evidence indicates that hemicellulose:lignin cross-linking has a substantial impact on plant cell wall recalcitrance. Engineering plant biomass with modified frequencies of cross-links will have significant impacts on biomass utilisation.O.M.T was a recipient of an iCASE studentship from the BBSRC and Novozymes (Reference BB/M015432/1). P.D. was supported by the Leverhulme Trust Centre for Natural Material Innovation and the OpenPlant Synthetic Biology Research Centre BB/L014130/1
Discovery of long-period variable stars in the very-metal-poor globular cluster M15
We present a search for long-period variable (LPV) stars among giant branch
stars in M15 which, at [Fe/H] ~ -2.3, is one of the most metal-poor Galactic
globular clusters. We use multi-colour optical photometry from the 0.6-m Keele
Thornton and 2-m Liverpool Telescopes. Variability of delta-V ~ 0.15 mag is
detected in K757 and K825 over unusually-long timescales of nearly a year,
making them the most metal-poor LPVs found in a Galactic globular cluster. K825
is placed on the long secondary period sequence, identified for metal-rich
LPVs, though no primary period is detectable. We discuss this variability in
the context of dust production and stellar evolution at low metallicity, using
additional spectra from the 6.5-m Magellan (Las Campanas) telescope. A lack of
dust production, despite the presence of gaseous mass loss raises questions
about the production of dust and the intra-cluster medium of this cluster.Comment: 13 pages, 9 figures, accepted by MNRA
Acute neuroinflammation induces AIS structural plasticity in a NOX2-dependent manner
Background Chronic microglia-mediated inflammation and oxidative stress are well-characterized underlying factors in neurodegenerative disease, whereby reactive inflammatory microglia enhance ROS production and impact neuronal integrity. Recently, it has been shown that during chronic inflammation, neuronal integrity is compromised through targeted disruption of the axon initial segment (AIS), the axonal domain critical for action potential initiation. AIS disruption was associated with contact by reactive inflammatory microglia which wrap around the AIS, increasing association with disease progression. While it is clear that chronic microglial inflammation and enhanced ROS production impact neuronal integrity, little is known about how acute microglial inflammation influences AIS stability. Here, we demonstrate that acute neuroinflammation induces AIS structural plasticity in a ROS-mediated and calpain-dependent manner. Methods C57BL/6J and NOX2−/− mice were given a single injection of lipopolysaccharide (LPS; 5 mg/kg) or vehicle (0.9% saline, 10 mL/kg) and analyzed at 6 h–2 weeks post-injection. Anti-inflammatory Didox (250 mg/kg) or vehicle (0.9% saline, 10 mL/kg) was administered beginning 24 h post-LPS injection and continued for 5 days; animals were analyzed 1 week post-injection. Microglial inflammation was assessed using immunohistochemistry (IHC) and RT-qPCR, and AIS integrity was quantitatively analyzed using ankyrinG immunolabeling. Data were statistically compared by one-way or two-way ANOVA where mean differences were significant as assessed using Tukey’s post hoc analysis. Results LPS-induced neuroinflammation, characterized by enhanced microglial inflammation and increased expression of ROS-producing enzymes, altered AIS protein clustering. Importantly, inflammation-induced AIS changes were reversed following resolution of microglial inflammation. Modulation of the inflammatory response using anti-inflammatory Didox, even after significant AIS disruption occurred, increased the rate of AIS recovery. qPCR and IHC analysis revealed that expression of microglial NOX2, a ROS-producing enzyme, was significantly increased correlating with AIS disruption. Furthermore, ablation of NOX2 prevented inflammation-induced AIS plasticity, suggesting that ROS drive AIS structural plasticity. Conclusions In the presence of acute microglial inflammation, the AIS undergoes an adaptive change that is capable of spontaneous recovery. Moreover, recovery can be therapeutically accelerated. Together, these findings underscore the dynamic capabilities of this domain in the presence of a pathological insult and provide evidence that the AIS is a viable therapeutic target
X-Ray Determination of the Variable Rate of Mass Accretion onto TW Hydrae
Diagnostics of electron temperature (T_e), electron density (n_e), and
hydrogen column density (N_H) from the Chandra High Energy Transmission Grating
spectrum of He-like Ne IX in TW Hydrae (TW Hya), in conjunction with a
classical accretion model, allow us to infer the accretion rate onto the star
directly from measurements of the accreting material. The new method introduces
the use of the absorption of Ne IX lines as a measure of the column density of
the intervening, accreting material. On average, the derived mass accretion
rate for TW Hya is 1.5 x 10^{-9} M_{\odot} yr^{-1}, for a stellar magnetic
field strength of 600 Gauss and a filling factor of 3.5%. Three individual
Chandra exposures show statistically significant differences in the Ne IX line
ratios, indicating changes in N_H, T_e, and n_e by factors of 0.28, 1.6, and
1.3, respectively. In exposures separated by 2.7 days, the observations
reported here suggest a five-fold reduction in the accretion rate. This
powerful new technique promises to substantially improve our understanding of
the accretion process in young stars
Understanding the Stigma and Feasibility of Opening a Safe Injection Facility in Baltimore City: A Qualitative Case Study
Supervised injection facilities (SIFs) are medically supervised facilities designed to provide a hygienic environment in which drug users can consume illicit drugs intravenously. SIFs can be cost saving, help to reduce transmission of disease, and decrease drug overdoses. There are no SIFs in the United States. In this study we used a multiple case study design to understand the stigma surrounding the use of a SIF and the feasibility of implementing the drug prevention strategy in Baltimore City by comparing experiences with opening a SIF in Sydney, Australia. We interviewed one healthcare worker at the Sydney SIF and ten community stakeholders in Baltimore City. Interviewees were asked about community stigma of SIFs, drug use, and feasibility of opening a SIF in Baltimore City. Six overarching themes were established including lack of trust, lack of public education, fear of police, concern about efficacy of harm reduction programs, drug user stigma, and concerns about implementation. Findings suggest that stigma surrounding drug use and drug users is the most important aspect in shaping the participant\u27s varied perceptions of SIFs. Participants believed that for any change to occur, there must be multi-tiered collaboration at the level of government, healthcare, community, and law enforcement
Combined ultraviolet studies of astronomical sources
Topics addressed include: Cygnus Loop; P Cygni profiles in dwarf novae; YY Gem; nova shells; HZ Herculis; activity cycles in cluster giants; Alpha Ori; metal deficient giant stars; ultraviolet spectra of symbiotic stars detected by the Very Large Array; time variability in symbiotic stars; blue galaxies; and quasistellar objects with X-ray spectra
Combined ultraviolet studies of astronomical sources
Ultraviolet studies of astronomical sources are discussed. Some studies utilized IVE data. Non-radiative shock at the edge of the Cygnses Loop, stellar flares, local interestellar medium, hot galaxies, stellar mass ejection, contact binaries, double quasars, and stellar chromosphere and coronae are discussed
- …