Assessment of Variation in Some Medicinal Plant Species Envisaged of Having the Potential for the Preservation of Herbal Products Using Some Statistical Models

A survey research was conducted on some medicinal plant species envisaged of having the potential for preservation of herbal preparations. The aim was to determine whether there is an interspecific relationship among some selected medicinal plant species using their total extracts as the basis for computerization. To this end, statistical model comprising the Duncan’s Multiple Range Test and Principal Component Analysis (PCA) were applied to the total extract obtained from the medicinal plant species harvested from Mampong- and Mamfe-Akuapem environs to determine the existence of variations. The results showed the existence of variation and some of the medicinal plant species were more influential or weighted more than others

<it>In vitro</it> antimicrobial activity of ethanolic fractions of <it>Cryptolepis sanguinolenta</it>

<p>Abstract</p> <p>Background</p> <p>Following claims that some plants have antimicrobial activities against infectious microbes, the <it>in vitro</it> antimicrobial activities of different solvent fractions of ethanolic extract of <it>Cryptolepis sanguinolenta</it> were evaluated against eight standard bacteria and clinical isolates.</p> <p>Methods</p> <p>The solvent partitioning protocol involving ethanol, petroleum ether, chloroform, ethyl acetate and water, was used to extract various fractions of dried pulverized <it>Cryptolepis sanguinolenta</it> roots. Qualitative phyto-constituents screening was performed on the ethanol extract, chloroform fraction and the water fraction. The Kirby Bauer disk diffusion method was employed to ascertain the antibiogram of the test organisms while the agar diffusion method was used to investigate the antimicrobial properties of the crude plant extracts. The microplate dilution method aided in finding the MICs while the MBCs were obtained by the method of Nester and friends. The SPSS 16.0 version was used to analyze the percentages of inhibitions and bactericidal activities.</p> <p>Results</p> <p>The phytochemical screening revealed the presence of alkaloids, reducing sugars, polyuronides, anthocyanosides and triterpenes. The ethanol extract inhibited 5 out of 8 (62.5%) of the standard organisms and 6 out of 8 (75%) clinical isolates. The petroleum ether fraction inhibited 4 out of 8 (50%) of the standard microbes and 1 out of 8 (12.5%) clinical isolates. It was also observed that the chloroform fraction inhibited the growth of all the organisms (100%). Average inhibition zones of 14.0 ± 1.0 mm to 24.67 ± 0.58 mm was seen in the ethyl acetate fraction which halted the growth of 3 (37.5%) of the standard organisms. Inhibition of 7 (87.5%) of standard strains and 6 (75%) of clinical isolates were observed in the water fraction. The chloroform fraction exhibited bactericidal activity against all the test organisms while the remaining fractions showed varying degrees of bacteriostatic activity.</p> <p>Conclusion</p> <p>The study confirmed that fractions of <it>Cryptolepis sanguinolenta</it> have antimicrobial activity. The chloroform fraction had the highest activity, followed by water, ethanol, petroleum ether and ethyl acetate respectively. Only the chloroform fraction exhibited bactericidal activity and further investigations are needed to ascertain its safety and prospects of drug development.</p

Detection of vaccine-derived poliovirus circulation by environmental surveillance in the absence of clinical cases

Background On August 25, 2019, the Noguchi Memorial Institute for Medical Research notified the confirmation of a circulating-vaccine-derived poliovirus type-2 (cVDPV2) from the Agbogbloshie environmental surveillance (AES) site, in the Greater Accra Region. A field investigation of the outbreak was conducted to describe the results of epidemiological and laboratory investigations, and control efforts. Methods We conducted a descriptive investigation, records review, and active-case-search. Caregivers were interviewed on the vaccination status of their children; knowledge, attitude, and practices on polio prevention; water, sanitation and hygiene practices, and health-seeking behaviors. Stool from healthy children <5 y and sewage samples were taken for laboratory diagnosis. Results cVDPV2 genetically similar to the cVDPV2 diagnosed recently in the Northern Region of Ghana and Nigeria was identified. 2019 half-year coverage of OPV and IPV was 22%. Fully immunized children were 49% (29/59). Most health workers (70%) had a fair knowledge of polio and acute flaccid paralysis (AFP). Forty-six percent of care-givers admitted to using the large drain linked to the site where the cVDPV2 was isolated as their place of convenience and disposing of the fecal matter of their children. No AFP case was identified. Stool samples from 40 healthy children yielded non-polio enteroviruses while 75% (3/4) of the additional sewage samples yielded cVDPV2. Conclusion cVDPV2 was isolated from the AES site. No AFP or poliovirus was identified from healthy children. There is a need to improve health workers’ knowledge on AFP and to address the dire sanitation conditions in the Agbogbloshie market and its environs