Вплив пробіотика “Імунобактерин-D” на біоценоз та розвиток шлунково-кишкового тракту телят

The paper presents the results of an experiment on the use of enzyme-probiotic supplement “Immunobacterin-D” for calves in the first two months of life. Probiotics are used in animal husbandry for the prevention and treatment of infectious diseases, increase immunity, correction of the microflora after antibiotic therapy, prevention of stress, increase growth. The study was conducted in a private farm in the Lyubar district of Zhytomyr region, which kept cattle of different ages. In a randomized controlled trial, two experimental groups of six animals were formed in each on the principle of analogues. One group was a control. Calves of the experimental group from the second day after birth were fed together with colostrum “Immunobacterin-D” at the rate of 3 grams per animal. For the first five days, the enzyme-probiotic supplement was given to the calves once a day during the morning feeding, and in subsequent studies, once every two days for a month. The results of the studies show that no case of diarrhea was registered among calves receiving the enzyme-probiotic supplement “Immunobacterin-D”. Among the animals of the control group, 66.7 % had digestive disorders – dyspepsia. Subsequently, such animals had a lower growth rate. Enzyme-probiotic supplement “Immunobacterin-D” also contains Bacillus Subthilis, Bacillus Licheniformis and enzymes and protease and lipase – it provides a polyvector effect in the digestive tract in general and scarring in particular. It was found that on the fourteenth day of feeding probiotic-containing drug “Immunobacterin-D” to calves of the experimental group, the number of bacteria probably increased by 83.5 %; ciliates – by 65.0 %; endodynomorphs – by 24.3 %. After one month of the experiment, the number of bacteria in the experimental group probably increased by 94.3 %; ciliates – by 40.5 %; endodynomorphs – by 26.7 %, compared with the control (P < 0.001). The obtained data of registration of the first chewing gum in calves prove that the components of the enzyme-probiotic supplement “Immunobacterin-D” accelerate the population of microflora and the development of scar digestion in calves 2.5 times compared to calves in the control group. Prospects for further research are to study the effect of enzyme-probiotic supplement “Immunobacterin-D” on the indicators of scar fermentation of calves.В роботі наведені результати експерименту щодо застосування ферментно-пробіотичної добавки “Імунобактерин-D” для телят перших двох місяців життя. Пробіотики використовують у тваринництві для профілактики та лікування інфекційних захворювань, підвищення імунітету, корекції мікрофлори після антибіотикотерапії, профілактики стресу, збільшення приростів. Дослідження проведено в умовах приватного господарства Любарського району Житомирської області, в якому утримували велику рогату худобу різних вікових груп. При рандомізованому контрольованому дослідженні формували дві дослідні групи по шість тварин у кожній за принципом аналогів. Одна група була контрольною. Телятам дослідної групи із другої доби після народження випоювали разом з молозивом “Імунобактерин-D” з розрахунку 3 грами на тварину. Перші п’ять діб ферментно-пробіотичну добавку задавали телятам один раз на добу під час вранішньої годівлі, в подальших дослідженнях – один раз на дві доби протягом місяця. Результати досліджень доводять, що серед телят, які отримували ферментно-пробіотичну добавку “Імунобактерин-D” не зареєстровано жодного випадку діареї. Серед тварин контрольної групи 66,7 % мали розлади травлення – диспепсію. В подальшому такі тварини мали меншу інтенсивність росту. Ферментно-пробіотична дабавка “Імунобактерин-D” містить і Bacillus Subthilis, Bacillus Licheniformis та ферменти і протеазу та ліпазу – це забезпечує полівекторну дію в травному тракті загалом та рубці зокрема. Встановлено, що на чотирнадцяту добу випоювання пробіотиковмісного лікарського засобу “Імунобактерин-D” телятам дослідної групи кількість бактерій вірогідно збільшилась на 83,5 %; інфузорій – на 65,0 %; ентодиноморфів – на 24,3 %. Через місяць експерименту у дослідній групі чисельність бактерій вірогідно збільшилась на 94,3 %; інфузорій – на 40,5 %; ентодиноморфів – на 26,7 %, порівняно з контролем (Р < 0,001). Отримані дані реєстрації першої жуйки у телят доводять, що складові ферментно-пробіотичної добавки “Імунобактерин-D” прискорюють заселення мікрофлорою та розвиток рубцевого травлення у телят в 2,5 разу порівняно із телятами контрольної групи. Перспективи подальших досліджень полягають у дослідженні впливу ферментно-пробіотичної добавки “Імунобактерин-D” на показники рубцевої ферментації телят.&nbsp

Forebrain NR2B Overexpression Facilitating the Prefrontal Cortex Long-Term Potentiation and Enhancing Working Memory Function in Mice

Prefrontal cortex plays an important role in working memory, attention regulation and behavioral inhibition. Its functions are associated with NMDA receptors. However, there is little information regarding the roles of NMDA receptor NR2B subunit in prefrontal cortical synaptic plasticity and prefrontal cortex-related working memory. Whether the up-regulation of NR2B subunit influences prefrontal cortical synaptic plasticity and working memory is not yet clear. In the present study, we measured prefrontal cortical synaptic plasticity and working memory function in NR2B overexpressing transgenic mice. In vitro electrophysiological data showed that overexpression of NR2B specifically in the forebrain region resulted in enhancement of prefrontal cortical long-term potentiation (LTP) but did not alter long-term depression (LTD). The enhanced LTP was completely abolished by a NR2B subunit selective antagonist, Ro25-6981, indicating that overexpression of NR2B subunit is responsible for enhanced LTP. In addition, NR2B transgenic mice exhibited better performance in a set of working memory paradigms including delay no-match-to-place T-maze, working memory version of water maze and odor span task. Our study provides evidence that NR2B subunit of NMDA receptor in prefrontal cortex is critical for prefrontal cortex LTP and prefrontal cortex-related working memory

The 5-Choice Continuous Performance Test: Evidence for a Translational Test of Vigilance for Mice

Attentional dysfunction is related to functional disability in patients with neuropsychiatric disorders such as schizophrenia, bipolar disorder, and Alzheimer's disease. Indeed, sustained attention/vigilance is among the leading targets for new medications designed to improve cognition in schizophrenia. Although vigilance is assessed frequently using the continuous performance test (CPT) in humans, few tests specifically assess vigilance in rodents.We describe the 5-choice CPT (5C-CPT), an elaboration of the 5-choice serial reaction (5CSR) task that includes non-signal trials, thus mimicking task parameters of human CPTs that use signal and non-signal events to assess vigilance. The performances of C57BL/6J and DBA/2J mice were assessed in the 5C-CPT to determine whether this task could differentiate between strains. C57BL/6J mice were also trained in the 5CSR task and a simple reaction-time (RT) task involving only one choice (1CRT task). We hypothesized that: 1) C57BL/6J performance would be superior to DBA/2J mice in the 5C-CPT as measured by the sensitivity index measure from signal detection theory; 2) a vigilance decrement would be observed in both strains; and 3) RTs would increase across tasks with increased attentional load (1CRT task<5CSR task<5C-CPT).C57BL/6J mice exhibited superior SI levels compared to DBA/2J mice, but with no difference in accuracy. A vigilance decrement was observed in both strains, which was more pronounced in DBA/2J mice and unaffected by response bias. Finally, we observed increased RTs with increased attentional load, such that 1CRT task<5CSR task<5C-CPT, consistent with human performance in simple RT, choice RT, and CPT tasks. Thus we have demonstrated construct validity for the 5C-CPT as a measure of vigilance that is analogous to human CPT studies

The NEWMEDS rodent touchscreen test battery for cognition relevant to schizophrenia.

RATIONALE: The NEWMEDS initiative (Novel Methods leading to New Medications in Depression and Schizophrenia, http://www.newmeds-europe.com ) is a large industrial-academic collaborative project aimed at developing new methods for drug discovery for schizophrenia. As part of this project, Work package 2 (WP02) has developed and validated a comprehensive battery of novel touchscreen tasks for rats and mice for assessing cognitive domains relevant to schizophrenia. OBJECTIVES: This article provides a review of the touchscreen battery of tasks for rats and mice for assessing cognitive domains relevant to schizophrenia and highlights validation data presented in several primary articles in this issue and elsewhere. METHODS: The battery consists of the five-choice serial reaction time task and a novel rodent continuous performance task for measuring attention, a three-stimulus visual reversal and the serial visual reversal task for measuring cognitive flexibility, novel non-matching to sample-based tasks for measuring spatial working memory and paired-associates learning for measuring long-term memory. RESULTS: The rodent (i.e. both rats and mice) touchscreen operant chamber and battery has high translational value across species due to its emphasis on construct as well as face validity. In addition, it offers cognitive profiling of models of diseases with cognitive symptoms (not limited to schizophrenia) through a battery approach, whereby multiple cognitive constructs can be measured using the same apparatus, enabling comparisons of performance across tasks. CONCLUSION: This battery of tests constitutes an extensive tool package for both model characterisation and pre-clinical drug discovery.This work was supported by the Innovative Medicine Initiative Joint Undertaking under grant agreement no. 115008 of which resources are composed of EFPIA in-kind contribution and financial contribution from the European Union’s Seventh Framework Programme (FP7/2007-2013). The authors thank Charlotte Oomen for valuable comments on the manuscript.This is the author accepted manuscript. The final version is available from Springer via http://dx.doi.org/10.1007/s00213-015-4007-

Population genomics of speciation and admixture

The application of population genomics to the understanding of speciation has led to the emerging field of speciation genomics. This has brought new insight into how divergence builds up within the genome during speciation and is also revealing the extent to which species can continue to exchange genetic material despite reproductive barriers. It is also providing powerful new approaches for linking genotype to phenotype in admixed populations. In this chapter, we give an overview of some of the methods that have been used and some of the novel insights gained. We also outline some of the pitfalls of the most commonly used methods and possible problems with interpretation of the results

Real-world study of children and young adults with myeloproliferative neoplasms: identifying risks and unmet needs

Myeloproliferative neoplasms (MPNs) are uncommon in children/young adults. Here, we present data on unselected patients diagnosed before 25 years of age included from 38 centers in 15 countries. Sequential patients were included. We identified 444 patients, with median follow-up 9.7 years (0-47.8). Forty-nine (11.1%) had a history of thrombosis at diagnosis, 49 new thrombotic events were recorded (1.16% patient per year [pt/y]), perihepatic vein thromboses were most frequent (47.6% venous events), and logistic regression identified JAK2V617F mutation (P = .016) and hyperviscosity symptoms (visual disturbances, dizziness, vertigo, headache) as risk factors (P = .040). New hemorrhagic events occurred in 44 patients (9.9%, 1.04% pt/y). Disease transformation occurred in 48 patients (10.9%, 1.13% pt/y), usually to myelofibrosis (7.5%) with splenomegaly as a novel risk factor for transformation in essential thrombocythemia (ET) (P= .000) in logistical regression. Eight deaths (1.8%) were recorded, 3 after allogeneic stem cell transplantation. Concerning conventional risk scores: International Prognostic Score for Essential Thrombocythemia-Thrombosis and new International Prognostic Score for Essential Thrombocythemia-Thrombosis differentiated ET patients in terms of thrombotic risk. Both scores identified high-risk patients with the same median thrombosis-free survival of 28.5 years. No contemporary scores were able to predict survival for young ET or polycythemia vera patients. Our data represents the largest real-world study of MPN patients age < 25 years at diagnosis. Rates of thrombotic events and transformation were higher than expected compared with the previous literature. Our study provides new and reliable information as a basis for prospective studies, trials, and development of harmonized international guidelines for the specific management of young patients with MPN

The genome sequence of segmental allotetraploid peanut Arachis hypogaea

Like many other crops, the cultivated peanut (Arachis hypogaea L.) is of hybrid origin and has a polyploid genome that contains essentially complete sets of chromosomes from two ancestral species. Here we report the genome sequence of peanut and show that after its polyploid origin, the genome has evolved through mobile-element activity, deletions and by the flow of genetic information between corresponding ancestral chromosomes (that is, homeologous recombination). Uniformity of patterns of homeologous recombination at the ends of chromosomes favors a single origin for cultivated peanut and its wild counterpart A. monticola. However, through much of the genome, homeologous recombination has created diversity. Using new polyploid hybrids made from the ancestral species, we show how this can generate phenotypic changes such as spontaneous changes in the color of the flowers. We suggest that diversity generated by these genetic mechanisms helped to favor the domestication of the polyploid A. hypogaea over other diploid Arachis species cultivated by humans

Dysregulation of specialized delay/interference-dependent working memory following loss of dysbindin-1A in schizophrenia-related phenotypes

Dysbindin-1, a protein that regulates aspects of early and late brain development, has been implicated in the pathobiology of schizophrenia. As the functional roles of the three major isoforms of dysbindin-1, (A, B, and C) remain unknown, we generated a novel mutant mouse, dys-1A -/-, with selective loss of dysbindin-1A and investigated schizophrenia-related phenotypes in both males and females. Loss of dysbindin-1A resulted in heightened initial exploration and disruption in subsequent habituation to a novel environment, together with heightened anxiety-related behavior in a stressful environment. Loss of dysbindin-1A was not associated with disruption of either long-term (olfactory) memory or spontaneous alternation behavior. However, dys-1A -/-showed enhancement in delay-dependent working memory under high levels of interference relative to controls, ie, impairment in sensitivity to the disruptive effect of such interference. These findings in dys-1A -/-provide the first evidence for differential functional roles for dysbindin-1A vs dysbindin-1C isoforms among phenotypes relevant to the pathobiology of schizophrenia. Future studies should investigate putative sex differences in these phenotypic effects

Improved reference genome of Aedes aegypti informs arbovirus vector control

Female Aedes aegypti mosquitoes infect more than 400 million people each year with dangerous viral pathogens including dengue, yellow fever, Zika and chikungunya. Progress in understanding the biology of mosquitoes and developing the tools to fight them has been slowed by the lack of a high-quality genome assembly. Here we combine diverse technologies to produce the markedly improved, fully re-annotated AaegL5 genome assembly, and demonstrate how it accelerates mosquito science. We anchored physical and cytogenetic maps, doubled the number of known chemosensory ionotropic receptors that guide mosquitoes to human hosts and egg-laying sites, provided further insight into the size and composition of the sex-determining M locus, and revealed copy-number variation among glutathione S-transferase genes that are important for insecticide resistance. Using high-resolution quantitative trait locus and population genomic analyses, we mapped new candidates for dengue vector competence and insecticide resistance. AaegL5 will catalyse new biological insights and intervention strategies to fight this deadly disease vector