Yeast cell death caused by nutrient desequilibrium during alcoholic fermentation is impacted by nitrogen sources

Nutrients availability is a key factor for controlling wine alcoholic fermentation. Among them, nitrogen has been identified as an essential parameter, controlling both the fermentation rate and the durationof the fermentation. However, nitrogen is not sufficient to ensure a correct fermentation and other nutrients such as vitamins and lipids, present in lower quantities, are required. Furthermore, we showed in a previous study that an excess of nitrogen combined with a depletion in certain micronutrients can lead to cell death and sluggish or stuck fermentation. In this study, we provide evidence of the mechanism controlling cell death and we show that all the nitrogen sources are not equivalent in the initiation of this phenomenon.Fermentations limited in oleic acid, pantothenic acid and nicotinic acid showed yeast cell death linked to a high nitrogen content. In each case, lowering the nitrogen level restored yeast viability. We evidenced that yeast cell lack of a correct stress response to those micronutrient starvations in presence of high levels of nitrogen. A transcriptional analysis showed a correct stress response suggesting that the lack of resistance originates from a post-transcriptional control mechanism. We then provide evidence that the nitrogen Tor/Sch9 signaling pathway is involved in triggering cell death.Yeast cell viability was then monitored and compared during fermentation starting at different nitrogen levels, with the addition of different nitrogen sources (19 amino acids and NH4+) and two different timing of NH4+ addition. We observed that cell death was triggered with different intensities.Yeast cell death associated to disequilibrium between micronutrients and nitrogen has been evidenced and its implication on fermentations highlighted. We showed a strong impact of both the nature of the nitrogen source and time of addition on yeast cell death and fermentation outcome

Sonification for 3D Printing Process Monitoring

In order to monitor a 3D printing industrial process in a context of sensory overload and potential inattentional deafness, we designed a sonification of the information sent in by the printer. This sonification focuses not only on proper communication of the system's state, but also on lowering the amount of stress usually induced by prolonged listening. To this end, we made use of a combination of synthetic and natural sounds whose perceptual properties were modulated according to the data influx using parameter mapping. Then an experiment was conducted on the recognition of various normal and abnormal behaviours, also allowing the participants to assess the amount of stress they experienced upon listening. The results are quite promising, but also highlight a confusing overlap in the natural sounds used, which will need to be fixed in future iterations. For now, tester opinion is mostly positive on the stressful aspect. However, listening times may need to be longer in further experimentation to better assess how stressful this sonification is

Local hyperhemia to heating is impaired in secondary Raynaud's phenomenon

Accurate and sensitive measurement techniques are a key issue in the quantification of the microvascular and endothelial dysfunction in systemic sclerosis (SSc). Thermal hyperhemia comprises two separate mechanisms: an initial peak that is axon reflex mediated; and a sustained plateau phase that is nitric oxide dependent. The main objective of our study was to test whether thermal hyperhemia in patients with SSc differed from that in patients with primary Raynaud's phenomenon (RP) and healthy controls. In a first study, we enrolled 20 patients suffering from SSc, 20 patients with primary RP and 20 healthy volunteers. All subjects were in a fasting state. Post-occlusive hyperhemia, 0.4 mg sublingual nitroglycerin challenge and thermal hyperhemia were performed using laser Doppler flowmetry on the distal pad of the third left finger. In a second study, thermal hyperhemia was performed in 10 patients with rheumatoid arthritis and 10 patients with primary RP. The thermal hyperhemia was dramatically altered in terms of amplitude and kinetics in patients with SSc. Whereas 19 healthy volunteers and 18 patients with primary RP exhibited the classic response, including an initial peak within the first 10 minutes followed by a nadir and a second peak, this occurred only in four of the SSc patients (p < 0.0001). The 10 minutes thermal peak was 43.4 (23.2 to 63), 42.6 (31 to 80.7) and 27 (14.7 to 51.4) mV/mm Hg in the healthy volunteers, primary RP and SSc groups, respectively (p = 0.01), while the 44°C thermal peak was 43.1 (21.3 to 62.1), 42.6 (31.6 to 74.3) and 25.4 (15 to 52.4) mV/mm Hg, respectively (p = 0.01). Thermal hyperhemia was more sensitive and specific than post-occlusive hyperhemia for differentiating SSc from primary RP. In patients with rheumatoid arthritis, thermal hyperhemia was also altered in terms of amplitude. Thermal hyperhemia is dramatically altered in patients with secondary RP in comparison with subjects with primary RP. Further studies are required to determine the mechanisms of this altered response, and whether it may provide additional information in a clinical setting

Luminous Infrared Starbursts in a Cluster of Galaxies

Analysing mid--infrared ISOCAM images of the cluster of galaxies J1888.16CL, we identified among its members several particularly active galaxies with total infrared luminosities well above 10^{11} Lsun. If powered by dust enshrouded starbursts, as suggested by their optical spectra, these Luminous Infrared Galaxies would exhibit star formation rates surprisingly high in the cluster environment. The triggering mechanism is unclear but could be tidal collisions within sub-structures or infalling groups.Comment: 5 pages, 2 figures, proceedings of the IAU Colloquium 195, "Outskirts of Galaxy Clusters: intense life in the suburbs", A. Diafero, e

Genetic background influences the 5XFAD Alzheimer\u27s disease mouse model brain proteome.

There is an urgent need to improve the translational validity of Alzheimer’s disease (AD) mouse models. Introducing genetic background diversity in AD mouse models has been proposed as a way to increase validity and enable the discovery of previously uncharacterized genetic contributions to AD susceptibility or resilience. However, the extent to which genetic background influences the mouse brain proteome and its perturbation in AD mouse models is unknown. In this study, we crossed the 5XFAD AD mouse model on a C57BL/6J (B6) inbred background with the DBA/2J (D2) inbred background and analyzed the effects of genetic background variation on the brain proteome in F1 progeny. Both genetic background and 5XFAD transgene insertion strongly affected protein variance in the hippocampus and cortex (n = 3,368 proteins). Protein co-expression network analysis identified 16 modules of highly co-expressed proteins common across the hippocampus and cortex in 5XFAD and non- transgenic mice. Among the modules strongly influenced by genetic background were those related to small molecule metabolism and ion transport. Modules strongly influenced by the 5XFAD transgene were related to lysosome/stress responses and neuronal synapse/signaling. The modules with the strongest relationship to human disease—neuronal synapse/signaling and lysosome/stress response—were not significantly influenced by genetic background. However, other modules in 5XFAD that were related to human disease, such as GABA synaptic signaling and mitochondrial membrane modules, were influenced by genetic background. Most disease-related modules were more strongly correlated with AD genotype in the hippocampus compared with the cortex. Our findings suggest that the genetic diversity introduced by crossing B6 and D2 inbred backgrounds influences proteomic changes related to disease in the 5XFAD model, and that proteomic analysis of other genetic backgrounds in transgenic and knock-in AD mouse models is warranted to capture the full range of molecular heterogeneity in genetically diverse models of AD

Diet of the Annamese langur (Trachypithecus margarita) (Elliot, 1909) at Takou Nature Reserve, Binh Thuan Province, Vietnam

The Annamese langur (Trachypithecus margarita) was described in 1909 but its ecology, including its feeding behaviour, is still largely unknown. Based on current knowledge of feeding ecology of many Asian langurs, T. margarita is expected to be a generalist herbivore, with seasonal variation in diet. Feeding behaviour of one habituated group of Annamese langurs at Takou Mountain was studied from October 2009 to September 2011. The dietary pattern of these langurs was found to be similar to other Trachypithecus species, which is characterised by a high amount of leaf consumption (54.4% immature leaves, 7.1% mature leaves) and complemented by fruits (29.9%). The Annamese langurs ate plant parts from 31 species belonging to 20 families on Takou Mountain where 689 plant species were known for the mountain. Moraceae is the most important family in the langur's diet with at least 10 species consumed and accounting for 41.1% of feeding time. Based on the current and previous feeding studies of Trachypithecus species, it could be concluded that the genus is a generalist folivore and the dietary pattern is similar among species.</p

Participant Blinding and Gastrointestinal Illness in a Randomized, Controlled Trial of an In-Home Drinking Water Intervention

We conducted a randomized, triple-blinded home drinking water intervention trial to determine if a large study could be undertaken while successfully blinding participants. Households were randomized 50:50 to use externally identical active or sham treatment devices. We measured the effectiveness of blinding of participants by using a published blinding index in which values >0.5 indicate successful blinding. The principal health outcome measured was “highly credible gastrointestinal illness” (HCGI). Participants (n=236) from 77 households were successfully blinded to their treatment assignment. At the end of the study, the blinding index was 0.64 (95% confidence interval 0.51-0.78). There were 103 episodes of HCGI during 10,790 person-days at risk in the sham group and 82 episodes during 11,380 person-days at risk in the active treatment group. The incidence rate ratio of disease (adjusted for the clustered sampling) was 1.32 (95% CI 0.75, 2.33) and the attributable risk was 0.24 (95% CI -0.33, 0.57). These data confirm that participants can be successfully blinded to treatment group assignment during a randomized trial of an in-home drinking water intervention

B-type natriuretic peptide and renal function in the diagnosis of heart failure: An analysis from the BNP multinational study

https://nsuworks.nova.edu/nsudigital_harrison/3343/thumbnail.jp