427 research outputs found

    Immunological survey of babesiosis ( Babesia peircei ) and toxoplasmosis in Jackass penguins in South Africa

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    Babesia peircei a été extrait d'érythrocytes nucléés provenant de Sphenicus demersus originaires d'Afrique du Sud infectés naturellement. Des fractions de Babesia peircei enrichies en glycoprotéines ont été obtenues par chromatographie sur colonne d'affinité concanavaline A-Sepharose et séparées par électrophorèse en gel de polyacrylamide-dodecylsulfate de sodium (SDS.PAGE). Quatorze bandes protéiques au minimum ont été observées (9, 11, 13, 20, 22, 23, 24, 43, 62, 90, 120, 204, et 205 kDa), la protéine majeure étant de 25 kDa. Des prélèvements sanguins provenant de 191 S. demersus adultes ont été testés par ELISA en utilisant les fractions de B. peircei enrichies en glycoprotéines pour détecter les IgG dirigées contre B. peircei. les prélèvements provenaient de trois groupes de manchots sauvages (n = 110), d'un groupe de manchots (n = 66) ayant été secourus après avoir été contaminés par une marée noire en mer et soignés à la Fondation Nationale Sud Africaine pour la Conservation des Oiseaux littoraux (SANCCOB), et d'un dernier groupe issu des manchots pensionnaires du SANCCOB (n = 15). La prévalence globale pour B. peircei était de 65 %, et la séropositivité moyenne s'échelonnait de 60 à 71 % parmi les cinq groupes de manchots. L'ELISA apparaissait spécifique pour les IgG dirigées contre B. peircei lorsque testée pour les IgG contre Haemoproteus columbae et les IgG contre le paludisme aviaire (Plasmodium relictum, et P. elongatum(, Les anticorps (Ac) dirigés contre Toxoplasma gondii ont été détectés par le test d'agglutination directe utilisant des tachyzoites de T. gondii tués. Tous les oiseaux étaient séronégatifs pour les Ac dirigés contre T. gondii. L'absence de manchots positifs pour T. gondii était due aux conditions sanitaires appropriées et aux méthodes de prévention contre T. gondii utilisées par le SANCCOB

    Toxoplasma, Toxocara and Tuberculosis co-infection in a four year old child

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    <p>Abstract</p> <p>Background</p> <p>Tuberculosis. toxocariasis and toxoplasmosis are among the common infectious causes of lymphadenitis in children. Cases of <it>Toxoplasma gondii </it>and <it>Toxocara spp </it>co-infection have been reported.</p> <p>Case Presentation</p> <p>This case report describes a co-infection of <it>Toxoplasma gondii</it>, <it>Toxocara </it>spp and tuberculosis in a child with chronic lymphadenopathy and eosinophilia.</p> <p>Conclusion</p> <p>The case report highlights two important points. First is the diagnostic challenges that are encountered by clinicians in tropical countries such as Sri Lanka, where lymphadenopathy and eosinophilia with a positive serology commonly point towards a parasitic infection. Secondly the importance of proper history taking and performing the Mantoux test as a first line investigation in a country where the incidence of tuberculosis is low, even in the absence of a positive contact history.</p

    Bio-nanotechnology application in wastewater treatment

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    The nanoparticles have received high interest in the field of medicine and water purification, however, the nanomaterials produced by chemical and physical methods are considered hazardous, expensive, and leave behind harmful substances to the environment. This chapter aimed to focus on green-synthesized nanoparticles and their medical applications. Moreover, the chapter highlighted the applicability of the metallic nanoparticles (MNPs) in the inactivation of microbial cells due to their high surface and small particle size. Modifying nanomaterials produced by green-methods is safe, inexpensive, and easy. Therefore, the control and modification of nanoparticles and their properties were also discussed

    Multilevel model to assess sources of variation in follicular growth close to the time of ovulation in women with normal fertility: a multicenter observational study

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    Mikolajczyk RT, Stanford JB, Ecochard R. Multilevel model to assess sources of variation in follicular growth close to the time of ovulation in women with normal fertility: a multicenter observational study. Reproductive Biology and Endocrinology. 2008;6(1): 61.Background: To assess the amount of variability in ovarian follicular growth rate and maximum follicular diameter related to different centers, women and cycles of the same women in a multicenter observational study of follicular growth. Methods: Secondary analysis of a prospective cohort study from eight centers in Europe. There were 533 ultrasound examinations in 282 cycles of 107 women with normal fertility. A random effects model with center, woman and cycle as hierarchical units of variation was used to analyze mean follicular diameter on days preceding ovulation. Results: Follicular growth did not differ by center. There was homogenous growth across women and cycles, and the maximum follicular diameter before ovulation varied substantially across cycles but not across women. Many (about 40%) women had small maximum follicular diameter on the day before ovulation (<19 mm). Pre-ovulatory cycle length was not related to maximum follicular diameter. Conclusion: In normal fecundity, there is a substantial variation in maximum follicular diameter from cycle to cycle based on variation in the duration of follicular development, but the variation could not be explained by different characteristics of different women. Explanation of variation in follicular growth has to be found on the cycle level

    Effects of metal-contaminated soils on the accumulation of heavy metals in gotu kola (Centella asiatica) and the potential health risks: a study in Peninsular Malaysia

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    Centella asiatica is a commonly used medicinal plant in Malaysia. As heavy metal accumulation in medicinal plants which are highly consumed by human is a serious issue, thus the assessment of heavy metals in C. asiatica is important for the safety of consumers. In this study, the heavy metal accumulation in C. asiatica and the potential health risks were investigated. Samples of C. asiatica and surface soils were collected from nine different sites around Peninsular Malaysia. The concentration of six heavy metals namely Cd, Cu, Ni, Fe, Pb and Zn were determined by air-acetylene flame atomic absorption spectrophotometer (AAS). The degree of anthropogenic influence was assessed by calculating the enrichment factor (EF) and index of geoaccumulation (Igeo). The heavy metal uptake into the plant was estimated through the calculation of translocation factor (TF), bioconcentration factor (BCF) and correlation study. Estimated daily intakes (EDI) and target hazard quotients (THQ) were used to determine the potential health risk of consuming C. asiatica. The results showed that the overall surface soil was polluted by Cd, Cu and Pb, while the uptake of Zn and Ni by the plants was high. The value of EDI and THQ showed that the potential of Pb toxicity in C. asiatica was high as well. As heavy metal accumulation was confirmed in C. asiatica, daily consumption of the plant derived from polluted sites in Malaysia was not recommended
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