Ecology, Biology and Genetics of <em>Millepora</em> Hydrocorals on Coral Reefs

Coral reefs are one of the most productive and diverse ecosystems on Earth. However, climate warming is occurring at an unprecedented rate and has negatively affected coral reefs worldwide. Evaluating the life history of reef-building species carries important implications for coral reef conservation. This chapter examines the taxonomy, biogeography, ecology, symbiosis, morphology and reproductive biology of Millepora hydrocorals, an important but relatively unstudied component of coral reefs. An emphasis is also placed on the influence of variable reef environments on Millepora life history traits, providing a fascinating opportunity to study the interplay between ecology and evolution. Special attention is given to ecological and evolutionary benefits of asexual versus sexual reproduction in the maintenance of genetic and phenotypic diversity. Lastly, this chapter discusses whether life-history strategies of Millepora hydrocorals and tolerance to different stressors can influence their ability to adapt and survive to future climate change, and other natural and anthropogenic disturbances

Protein-coding variants implicate novel genes related to lipid homeostasis contributing to body-fat distribution.

Body-fat distribution is a risk factor for adverse cardiovascular health consequences. We analyzed the association of body-fat distribution, assessed by waist-to-hip ratio adjusted for body mass index, with 228,985 predicted coding and splice site variants available on exome arrays in up to 344,369 individuals from five major ancestries (discovery) and 132,177 European-ancestry individuals (validation). We identified 15 common (minor allele frequency, MAF ≥5%) and nine low-frequency or rare (MAF &lt;5%) coding novel variants. Pathway/gene set enrichment analyses identified lipid particle, adiponectin, abnormal white adipose tissue physiology and bone development and morphology as important contributors to fat distribution, while cross-trait associations highlight cardiometabolic traits. In functional follow-up analyses, specifically in Drosophila RNAi-knockdowns, we observed a significant increase in the total body triglyceride levels for two genes (DNAH10 and PLXND1). We implicate novel genes in fat distribution, stressing the importance of interrogating low-frequency and protein-coding variants

Modelling human choices: MADeM and decision‑making

Research supported by FAPESP 2015/50122-0 and DFG-GRTK 1740/2. RP and AR are also part of the Research, Innovation and Dissemination Center for Neuromathematics FAPESP grant (2013/07699-0). RP is supported by a FAPESP scholarship (2013/25667-8). ACR is partially supported by a CNPq fellowship (grant 306251/2014-0)

Population structure of the hydrocoral Millepora platyphylla in habitats experiencing different flow regimes in Moorea, French Polynesia.

While the fire coral Millepora platyphylla is an important component of Indo-Pacific reefs, where it thrives in a wide range of environments, the ecological and biological processes driving its distribution and population structure are not well understood. Here, we quantified this species' population structure in five habitats with contrasting hydrodynamic regimes in Moorea, French Polynesia; two in the fore reef: mid and upper slopes, and three in the lagoon: back, fringing and patch reefs. A total of 3651 colonies of fire corals were mapped and measured over 45,000 m2 of surveyed reef. Due to the species' sensitivity to fragmentation in response to strong water movement, hydrodynamic conditions (e.g. waves, pass and lagoonal circulation) corresponded to marked differences in colony size distributions, morphology and recruitment dynamics among habitats. The size structure varied among reef habitats with higher proportions of larger colonies in calm nearshore reefs (fringing and patch reefs), while populations were dominated by smaller colonies in the exposed fore reefs. The highest densities of fire corals were recorded in fore reef habitats (0.12-0.20 n.m-2) where the proportion of recruits and juveniles was higher at mid slope populations (49.3%) than on the upper slope near where waves break (29.0%). In the latter habitat, most colonies grew as vertical sheets on encrusting bases making them more vulnerable to colony fragmentation, whereas fire corals were encrusting or massive in all other habitats. The lowest densities of M. platyphylla occurred in lagoonal habitats (0.02-0.04 n.m-2) characterized by a combination of low water movement and other physical and biological stressors. This study reports the first evidence of population structure of fire corals in two common reef environments and illustrates the importance of water flow in driving population dynamic processes of these reef-building species

Genetic diversity and differentiation in reef-building Millepora species, as revealed by cross-species amplification of fifteen novel microsatellite loci

Quantifying the genetic diversity in natural populations is crucial to address ecological and evolutionary questions. Despite recent advances in whole-genome sequencing, microsatellite markers have remained one of the most powerful tools for a myriad of population genetic approaches. Here, we used the 454 sequencing technique to develop microsatellite loci in the fire coral Millepora platyphylla, an important reef-builder of Indo-Pacific reefs. We tested the cross-species amplification of these loci in five other species of the genus Millepora and analysed its success in correlation with the genetic distances between species using mitochondrial 16S sequences. We succeeded in discovering fifteen microsatellite loci in our target species M. platyphylla, among which twelve were polymorphic with 2–13 alleles and a mean observed heterozygosity of 0.411. Cross-species amplification in the five other Millepora species revealed a high probability of amplification success (71%) and polymorphism (59%) of the loci. Our results show no evidence of decreased heterozygosity with increasing genetic distance. However, only one locus enabled measures of genetic diversity in the Caribbean species M. complanata due to high proportions of null alleles for most of the microsatellites. This result indicates that our novel markers may only be useful for the Indo-Pacific species of Millepora. Measures of genetic diversity revealed significant linkage disequilibrium, moderate levels of observed heterozygosity (0.323–0.496) and heterozygote deficiencies for the Indo-Pacific species. The accessibility to new polymorphic microsatellite markers for hydrozoan Millepora species creates new opportunities for future research on processes driving the complexity of their colonisation success on many Indo-Pacific reefs

Morphology of <i>Millepora platyphylla</i> adult colonies across the five surveyed habitats.

<p>Proportions of colonies with encrusting, sheet tree and massive morphology were averaged by habitats and error bars show the standard error for transect replicates. Similar letters over each set of bars indicate no statistical difference in post-hoc comparisons for a given morphology among habitats (<i>P ></i> 0.05). See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0173513#pone.0173513.g002" target="_blank">Fig 2</a> for photos of each of the morphologies.</p

Index describing the population size structure and recruitment for <i>Millepora platyphylla</i> across the five habitats surveyed.

<p>Index describing the population size structure and recruitment for <i>Millepora platyphylla</i> across the five habitats surveyed.</p

Aerial views of the locations of each transect in the five surveyed habitats in Moorea, French Polynesia.

<p>The names of these surveyed locations are: (A) Tiahura, (B) Papetoai, (C) Cook’s Bay and (D) Temae. Map data WorldView–2, Digital Globe.</p

Morphologies of <i>Millepora platyphylla</i> colonies in habitats experiencing contrasting hydrodynamic regimes.

<p>(A) Encrusting wave-tolerant morphology in the mid slope, a fore reef habitat at 13 m; (B) sheet tree morphology vulnerable to wave-induced breakage in the upper slope, a fore reef habitat at 6 m and (C) massive wave-tolerant morphology in the patch reef, a lagoonal habitat (photo is courtesy of Gilles Siu).</p