11 research outputs found
Action Of Essential Oils From Brazilian Native And Exotic Medicinal Species On Oral Biofilms
Background: Essential oils (EO) obtained from twenty medicinal and aromatic plants were evaluated for their antimicrobial activity against the oral pathogens Candida albicans, Fusobacterium nucleatum, Porphyromonas gingivalis, Streptococcus sanguis and Streptococcus mitis. Methods: The antimicrobial activity of the EO was evaluates by microdilution method determining Minimal Inhibitory Concentration. Chemical analysis of the oils compounds was performed by Gas chromatography-mass spectrometry (CG-MS). The most active EO were also investigated as to their actions on the biolfilm formation. Results: The most of the essential oils (EO) presented moderate to strong antimicrobial activity against the oral pathogens (MIC - Minimal Inhibitory Concentrations values between 0.007 and 1.00mg/mL). The essential oil from Coriandrum sativum inhibited all oral species with MIC values from 0.007 to 0.250mg/mL, and MBC/MFC (Minimal Bactericidal/Fungicidal Concentrations) from 0.015 to 0.500mg/mL. On the other hand the essential oil of C. articulatus inhibited 63.96% of S. sanguis biofilm formation. Through Scanning Eletronic Microscopy (SEM) images no changes were observed in cell morphology, despite a decrease in biofilm formation and changes on biofilm structure. Chemical analysis by Gas Chromatography - Mass Spectrometry (GC-MS) of the C. sativum essential oil revealed major compounds derivatives from alcohols and aldehydes, while Cyperus articulatus and Aloysia gratissima (EOs) presented mono and sesquiterpenes. Conclusions: In conclusion, the crude oil from C. articulatus exhibited the best results of antimicrobial activity e ability to control biofilm formation. The chemical analysis showed the presence of terpenes and monoterpenes such as a-pinene, a-bulnesene and copaene. The reduction of biofilms formation was confirmed from SEM images. 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Antimicrobial Activity And Differentiation Of Anthocyanin Profiles Of Rabbiteye And Highbush Blueberries Using Hplc–dad–esi-msn And Multivariate Analysis
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)This study aims to perform in vitro analyses of the antimicrobial activity of ten blueberry cultivars, to characterize the anthocyanin composition of these fruits, and to use multivariate analysis to compare the results. All blueberry cultivars presented anti-Escherichia coli activity. Furthermore, the complete series of six glycosides (galactoside, glucoside, arabinoside, xyloside, acetylgalactoside and acetylglucoside) of five anthocyanidins (delphinidin, cyanidin, petunidin, peonidin, and malvidin) were detected, together with six malonyl derivatives of the main anthocyanins. The rabbiteye cultivars showed higher proportions of cyanidin 3-galactoside than the highbush cultivars, the latter accounting for higher proportions of the 3-arabinosides of delphinidin and malvidin. The total anthocyanin content was between 10.8 and 26.5 g/kg of dry weight, and the concentration depended on the size of the berries, with the smaller rabbiteye cultivars exhibiting higher levels of anthocyanins. © 2016 Elsevier Ltd26506516CAPES, Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorESF, Fondo Social EuropeoUNICAMP, Universidade Estadual de CampinasCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Prenylated Flavonoids From Roots Of Dahlstedtia Glaziovii (fabaceae)
A phytochemical study of roots of Dahlstedtia glaziovii (Fabaceae) furnished a new dibenzoylmethane (glaziovione), along with eighteen known compounds. Their structures were determined through 1D and 2D nuclear magnetic resonance (NMR) (heteronuclear single quantum coherence, HSQC, and heteronuclear multiple bond correlation, HMBC) and high-resolution mass spectrometry (HRMS) spectral analyses. The antiproliferative activity was investigated for the crude extracts, the dibenzoylmethanes 2'-methoxy-8-(a-a-dimethylallyl)-furano- [4",5":3',4']- dibenzoylmethane, 3,4-methylenedioxy-2'-methoxy-8-(a-a- dimethylallyl)-furano-[4",5":3',4']- dibenzoylmethane and pongamol, and the flavones lanceolatin B, karanjin, pongapin and 3',4'-methylenedioxy-2'', 2''-dimethylpyrano-[5'',6'':8,7]-flavone. The dibenzoylmethanes were more active than the flavones.The extracts were evaluated for their antimicrobial effects, but none was shown to be active. © 2014 Sociedade Brasileira de Química.2569951001Da Silva, M.J., Queiroz, L.P., Tozzi, A.M.G.A., Lewis, G.P., Souza, A.P., (2012) Taxon, 61, p. 93Garcez, F.R., Scramin, S., Do Nascimento, M., Mors, W.B., (1988) Phytochemistry, 27, p. 1079Pietta, P.G., (2000) J. Nat. Prod, 63, p. 1035Shirley, B.W., (1996) Trends Plant Sci, 11, p. 377Guaratini, T., Callejon, D.R., Pires, D.C., Lopes, J.N.C., Lima, L.M., Neto, D.G., Sustovich, C., Lopes, N.P., (2009) Quim. Nova, 32, p. 717Cushnie, T.P.T., Lamb, A.J., (2005) Int. J. Antimicrob. Agents, 26, p. 343Ko, H., Tsao, L.-T., Yu, K.-L., Liu, C.-T., Wang, J.-P., Lin, C.-N., (2003) Bioorg. Med. Chem, 11, p. 105Pan, L., Chai, H., Kinghorn, A.D., (2010) Phytochem. Lett, 3, p. 1Harborne, J.B., Williams, C.A., (2000) Phytochemistry, 55, p. 481Spencer, J.P.E., (2009) Chem. Soc. Rev, 38, p. 1152López, S., Castelli, M., Zacchino, S., Domínguez, J., Lobo, G., Charris-Charris, J., Cortes, G.J.C., Enriz, R.D., (2001) Bioorg. Med. Chem, 9, p. 1999Havsteen, B.H., (2002) Pharmacol. Ther, 96, p. 67Clinical and Laboratory Standards Institute (CLSI document M27-A2, , Método de Referência para Testes de Diluição em Caldo para Determinação da Sensibilidade de Leveduras à Terapia AntifúngicaNorma Aprovada, 2a ed.CLSI: Wayne, PA, 2002Clinical and Laboratory Standards Institute (CLSI document M7-A6, , Metodologia dos Testes de Sensibilidade a Agentes Antimicrobianos por Diluição para Bactéria de Crescimento AeróbicoNorma Aprovada, 6a ed.CLSI: Wayne, PA, 2003Monks, A., Scudeiro, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Boyd, M., (1991) J. Natl. Cancer Inst, 83, p. 757Magalhães, A.F., Tozzi, A.M.G.A., Magalhães, E.G., Blanco, I.S., Nogueira, M.A., (1997) Phytochemistry, 46, p. 1029Talapatra, S.K., Mallik, A.K., Talapatra, B., (1980) Phytochemistry, 19, p. 1199Magalhães, A.F., Tozzi, A.M.G.A., Magalhães, E.G., Sannomiya, M., Soriano, M.D.P.C., Perez, M.A.F., (2007) An. Acad. Bras. Cienc, 79, p. 351Sharma, P., Seshadri, T.R., Mukerjee, S.K., (1973) Indian J. Chem, 11, p. 985Pathak, V.P., Saini, T.R., Khanna, R.N., (1983) Phytochemistry, 22, p. 1303Do Nascimento, M., Mors, W.B., (1972) Phytochemistry, 11, p. 3023Gupta, R.K., Krishnamurti, M., (1976) Phytochemistry, 15, p. 2011Tanaka, T., Iinuma, M., Yuki, K., Fujii, Y., Mizuno, M., (1992) Phytochemistry, 31, p. 993Mahey, S., Sharma, P., Seshadri, T.R., Mukerjee, S.K., (1972) Indian J. Chem, 10, p. 585Khanna, R.N., Seshadri, T.R., (1963) Tetrahedron, 19, p. 219Mukerje, S.K., Sarkar, S.C., Sewadri, T.R., (1969) Tetrahedron, 25, p. 1063Vasconcelos, J.N., Santiago, G.M.P., Lima, J.Q., Mafezoli, J., De Lemos, T.L.G., Da Silva, F.R.L., Lima, M.A.S., Cesarin-Sobrinho, D., (2012) Quim. Nova, 35, p. 1097Magalhães, A.F., Tozzi, A.M.G.A., Sales, B.H.L.N., Magalhães, E.G., (1996) Phytochemistry, 42, p. 1459Magalhães, A.F., Tozzi, A.M.G.A., Magalhães, E.G., Blanco, I.S., Soriano, M.D.P.C., (2004) An. Acad. Bras. Cienc, 76, p. 65
Integrated stoichiometric, thermodynamic and kinetic modelling of steady state metabolism
The quantitative analysis of biochemical reactions and metabolites is at frontier of biological sciences. The recent availability of high-throughput technology data sets in biology has paved the way for new modelling approaches at various levels of complexity including the metabolome of a cell or an organism. Understanding the metabolism of a single cell and multi-cell organism will provide the knowledge for the rational design of growth conditions to produce commercially valuable reagents in biotechnology. Here, we demonstrate how equations representing steady state mass conservation, energy conservation, the second law of thermodynamics, and reversible enzyme kinetics can be formulated as a single system of linear equalities and inequalities, in addition to linear equalities on exponential variables. Even though the feasible set is non-convex, the reformulation is exact and amenable to large-scale numerical analysis, a prerequisite for computationally feasible genome scale modelling. Integrating flux, concentration and kinetic variables in a unified constraint-based formulation is aimed at increasing the quantitative predictive capacity of flux balance analysis. Incorporation of experimental and theoretical bounds on thermodynamic and kinetic variables ensures that the predicted steady state fluxes are both thermodynamically and biochemically feasible. The resulting in silico predictions are tested against fluxomic data for central metabolism in Escherichia coli and compare favourably with in silico prediction by flux balance analysis.National University of Ireland, Galway, Science Faculty Fellowship. I.T. was supported by NIH grant Grant 5R01GM057089-11.Deposited by bulk impor
Quantitative Assignment of Reaction Directionality in a Multicompartmental Human Metabolic Reconstruction
Morfoanatomia e histoquímica da semente de sororoca (Phenakospermum guyannense (Rich.) Endl. - Strelitziaceae)
Phenakospermum guyannense, popularly known in the Amazon as sororoca, is usually found along rivers and in ombrophilous environments. The objective of this study was to describe the morpho-anatomy and histochemistry of mature seeds of P. guyannense collected at the Urubuí Waterfall, Presidente Figueiredo, Amazonas state, Brazil. Seed anatomy was studied using a light and a scanning electron microscopes (SEM). Histochemical tests were performed to identify phenolic compounds, starch, protein and lipids. The mature seed of P. guyannense is stenospermic, with a black seed coat composed of several layers of different cell types, with most containing phenolic compounds. The hilum is punctiform, surrounded by cells, which form the aryl. The endosperm is solid, formed by tetrahedral cells containing starch and protein. The embryo, which is cylindrical and located in the proximal region, is basal capitate, with cells containing lipids and proteins and is composed of a slightly dilated hypocotyl-radicle axis. The haustorium is flattened and located in the distal region