Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility

Citation: Huss AR, Schumacher LL, Cochrane RA, Poulsen E, Bai J, Woodworth JC, et al. (2017) Elimination of Porcine Epidemic Diarrhea Virus in an Animal Feed Manufacturing Facility. PLoS ONE 12(1): e0169612. doi:10.1371/journal.pone.0169612Porcine Epidemic Diarrhea Virus (PEDV) was the first virus of wide scale concern to be linked to possible transmission by livestock feed or ingredients. Measures to exclude pathogens, prevent cross-contamination, and actively reduce the pathogenic load of feed and ingredients are being developed. However, research thus far has focused on the role of chemicals or thermal treatment to reduce the RNA in the actual feedstuffs, and has not addressed potential residual contamination within the manufacturing facility that may lead to continuous contamination of finished feeds. The purpose of this experiment was to evaluate the use of a standardized protocol to sanitize an animal feed manufacturing facility contaminated with PEDV. Environmental swabs were collected throughout the facility during the manufacturing of a swine diet inoculated with PEDV. To monitor facility contamination of the virus, swabs were collected at: 1) baseline prior to inoculation, 2) after production of the inoculated feed, 3) after application of a quaternary ammonium-glutaraldehyde blend cleaner, 4) after application of a sodium hypochlorite sanitizing solution, and 5) after facility heat-up to 60°C for 48 hours. Decontamination step, surface, type, zone and their interactions were all found to impact the quantity of detectable PEDV RNA (P < 0.05). As expected, all samples collected from equipment surfaces contained PEDV RNA after production of the contaminated feed. Additionally, the majority of samples collected from non-direct feed contact surfaces were also positive for PEDV RNA after the production of the contaminated feed, emphasizing the potential role dust plays in cross-contamination of pathogen throughout a manufacturing facility. Application of the cleaner, sanitizer, and heat were effective at reducing PEDV genomic material (P < 0.05), but did not completely eliminate it

Effects of different diet alternatives to replace the use of pharmacological levels of zinc on growth performance and fecal dry matter of weanling pigs

A total of 300 weanling pigs (Line 400 × 200, DNA, Columbus, NE, initially 4.83 kg) were used in a 46-d trial to evaluate the effects of different nutritional strategies to replace pharmacological levels of Zn, provided by zinc oxide (ZnO), in nursery diets on growth performance and fecal dry matter (DM). Six treatments with 10 replicate pens per treatment and 5 pigs per pen were used. Diets consisted of: (1) positive control (ZnO providing 3,000 mg/kg added Zn from d 0 to 7 and 2,000 mg/kg added Zn from d 8 to 25 and 21% crude protein, CP); (2) negative control (NC; no added ZnO); (3) NC plus 1.2% Na diformate; (4) NC with 4% coarse ground wheat bran; (5) NC but formulated to 18% CP; and (6) the combination of NC with 18% CP, 1.2% Na diformate, and 4% coarse ground wheat bran. The diets formulated to 18% CP contained 1.2% standardized ileal digestible (SID) Lys from d 0 to 25, whereas the 21% CP diets contained 1.4% SID Lys from d 0 to 7 and 1.35% SID Lys from d 7 to 25. From d 25 to 46, all pigs were fed a common diet. From d 0 to 7, no differences in any variables were observed between treatments. From d 7 to 25, pigs fed the diet with added ZnO had greater (P < 0.01) average daily gain (ADG) and average daily feed intake (ADFI) than all other treatments. Pigs fed the diet formulated to 18% CP had decreased (P < 0.01) ADG when compared with pigs fed the other diets. From d 25 to 46, no previous treatment effects on ADG or gain to feed ratio (G:F) were observed. Overall (d 0 to 46), pigs fed the diet with added ZnO from d 0 to 25 had greater (P < 0.01) ADG, ADFI, and final body weight than pigs fed added Na Diformate, or 4% coarse ground wheat bran, or with the 18% CP diet, or with pigs fed the combination of the additives intermediate. There was no evidence for differences in overall G:F. Pigs fed the NC diet had the lowest fecal DM and highest fecal scores (P < 0.05), indicating the greatest incidence of loose stools. Pigs fed added ZnO had greater fecal DM than pigs fed the NC, 4% added wheat bran, or 18% CP diets, or with pigs fed the combination of additives intermediate (P < 0.01). These results suggest that adding pharmacological levels of Zn from ZnO improves nursery pig performance and increases DM content of feces when compared with pigs fed diets with either Na diformate, 4% course wheat bran, or 18% CP alone. However, a combination of all three alternatives appeared to be additive and partially restored growth performance similar to adding pharmacological levels of Zn

A Multi-Trial Analysis Evaluating the Effects of Pharmacological Levels of IntelliBond Copper on Growing-Finishing Pig Growth Performance and Carcass Characteristics

A multi-trial analysis was conducted to evaluate the effects of pharmacological levels of added IntelliBond Copper (IBC) on growing-finishing pig growth performance and carcass characteristics compared to pigs fed control diets containing typical additions of copper from a trace mineral premix. Data with 331 observations within 8 trials were included in the final database. Inclusion rates for IBC were either 150 (7 trials), or 200 (1 trial) ppm. Pigs fed IBC throughout the entire grow-finish period had greater (P \u3c 0.05) overall ADG, ADFI, and final BW and tended (P = 0.085) to be more efficient than pigs fed control diets. There was no evidence for differences (P \u3e 0.10) between dietary treatments for the percentage of pigs marketed. Pigs fed IBC also had (P \u3c 0.05) heavier HCW and greater carcass ADG compared to pigs fed a control diet. There were no differences (P \u3e 0.10) between pigs fed the control or IBC diets for carcass characteristics, carcass yield, and carcass feed efficiency. In conclusion, the addition of IBC at growth promotion levels throughout the entire growing-finishing period increased final BW, HCW, and ADG on a live and carcass basis

Feed Mitigant Efficacy for Control of Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus when Inoculated Alone or Together in Feed

Research has demonstrated that swine feed can be a fomite for viral transmission and feed additives can reduce viral contamination. Therefore, the objective of this study was to evaluate two feed additives in feed contaminated with PEDV or PRRSV. Feed additives included: no treatment, 0.33% commercial formaldehyde-based product, and 0.50% medium chain fatty acids (MCFA) blend. Feed samples were inoculated with PEDV and PRRSV alone or together at an inoculation concentration of 106 TCID50/g for each virus. Once inoculated, feed was stored at room temperature for 24 h before analyzing via qRT-PCR. For samples inoculated with PEDV or PRRSV alone, a quantitative real time reverse transcription PCR (qRT-PCR) assay was used, which was designed to detect PEDV or PRRSV nucleic acid. For co-inoculated samples, an assay was designed to detect PEDV and PRRSV within a single assay. For PEDV alone, there was marginally significant evidence that feed additives resulted in differences in cycle threshold (Ct) value (P = 0.052), but no evidence was observed for pairwise differences. For PRRSV alone, formaldehyde increased Ct compared to the untreated control and MCFA treatment (P \u3c 0.05). For co-infection of PRRSV and PEDV, MCFA and formaldehyde increased Ct (P \u3c 0.05) in comparison to non-treated feed. In summary, formaldehyde increased Ct values in feed when contaminated with PRRSV while both feed additives increased Ct in feed when co-inoculated with PRRSV and PEDV. This study also provided evidence that the co-inoculation model can effectively evaluate mitigants

Feed Mitigant Efficacy for Control of Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus when Inoculated Alone or Together in Feed

Research has demonstrated that swine feed can be a fomite for viral transmission and feed additives can reduce viral contamination. Therefore, the objective of this study was to evaluate two feed additives in feed contaminated with PEDV or PRRSV. Feed additives included: no treatment, 0.33% commercial formaldehyde-based product, and 0.50% medium chain fatty acids (MCFA) blend. Feed samples were inoculated with PEDV and PRRSV alone or together at an inoculation concentration of 106 TCID50/g for each virus. Once inoculated, feed was stored at room temperature for 24 h before analyzing via qRT-PCR. For samples inoculated with PEDV or PRRSV alone, a quantitative real time reverse transcription PCR (qRT-PCR) assay was used, which was designed to detect PEDV or PRRSV nucleic acid. For co-inoculated samples, an assay was designed to detect PEDV and PRRSV within a single assay. For PEDV alone, there was marginally significant evidence that feed additives resulted in differences in cycle threshold (Ct) value (P = 0.052), but no evidence was observed for pairwise differences. For PRRSV alone, formaldehyde increased Ct compared to the untreated control and MCFA treatment (P \u3c 0.05). For co-infection of PRRSV and PEDV, MCFA and formaldehyde increased Ct (P \u3c 0.05) in comparison to non-treated feed. In summary, formaldehyde increased Ct values in feed when contaminated with PRRSV while both feed additives increased Ct in feed when co-inoculated with PRRSV and PEDV. This study also provided evidence that the co-inoculation model can effectively evaluate mitigants

Effect of Parity and Stage of Gestation on Maternal Growth and Feed Efficiency of Gestating Sows

The objective of this study was to evaluate the effect of parity and stage of gestation on maternal weight gain and efficiency of feed use in group-housed gestating sows from a commercial sow farm. A total of 712 females (Camborough, PIC, Hendersonville, TN) were group-housed from d 5 to 112 of gestation and individually fed with electronic sow feeders (ESF). Feed intake and BW were recorded daily throughout gestation via the ESF and a scale located in an alleyway just after sows exited the feeding station. Gilts (parity 1) and sows received 6.5 and 7.3 Mcal ME per d. Maternal weight gain, not including products of conceptus, and feed efficiency were predicted using a series of equations to model nutrient utilization in gestation. Data were divided into 3 parity groups: 1, 2, and 3+, and gestation was divided into 3 periods: d 5 to 39, 40 to 74, and 75 to 109.After dividing energy requirements into tissue pools for maintenance, growth (maternal protein and fat deposition) and products of conceptus, the greatest portion of the energy requirement was for maintenance and maternal growth. The predicted energy used for maternal protein and fat deposition decreased (P \u3c 0.05) in each period of gestation, regardless of parity group. Parity 2 sows had the greatest (P \u3c 0.05) energy use for maternal protein and fat deposition in all stages of gestation while parity 1 sows had a negative energy balance during the final stage of gestation. Parity 1 sow maternal BW increased (P \u3c 0.05) in each period of gestation; however, parity 2 and 3+ sow maternal BW remained static after d 74 of gestation. Parity 3+ sows had the greatest (P \u3c 0.05) maternal BW throughout the course of gestation in comparison to other parity groups. Regardless of parity, maternal ADG decreased (P \u3c 0.05) from d 39 to 74 before increasing (P \u3c 0.05) during the final stage of gestation. Parity 1 sows had the greatest (P \u3c 0.05) ADG in all gestation periods. Parity 1 sow G:F decreased (P \u3c 0.05) in each sequential period of gestation. Parity 2 and 3+ sow G:F decreased (P \u3c 0.05) from d 39 to 74 but improved (P \u3c 0.05) during the final period of gestation. Parity 1 sow G:F was greater than parity 2 and 3+ sows in most gestation periods. Overall, this study demonstrates how feed usage, stage of gestation, and parity affect sow maternal BW and tissue pool composition in highly prolific sows

Feed Efficiency: An Assessment of Current Knowledge from a Voluntary Subsample of the Swine Industry

A voluntary sample of pork producers and advisers to the swine industry were surveyed about feed efficiency. The questionnaire was designed to accomplish three objectives: (a) determine the level of knowledge related to feed efficiency topics, (b) identify production practices used that influence feed efficiency, and (c) identify information gaps requiring additional knowledge to further improve feed efficiency. Results suggest that many practices that improve feed efficiency are used in production, but gaps in information and knowledge exist across demographics of respondents. Extension education should be expanded to provide more information in an easy-to-access format for the swine industry