Isolation, identification and anti-cancer activity of minor alkaloids from Triclisia subcordata

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Evidence for colony-specific differences in chemical mimicry in the parasitic mite Varroa destructor

In social insects, the integrity of a colony is maintained by recognising and removing aliens. Nest-mates use chemical cues on the cuticle of the individual they encounter to determine whether or not it is part of the colony. Parasites have evolved to take advantage of this recognition system by mimicking these chemical cues to gain entry to the colony and therefore avoid being attacked by the host during their stay. Some of these parasites imitate the odour of a particular sub-group of colony members, such as pupae, which makes it more likely that they are accepted into the colony, whereas others mimic the adult colony odour. The ectoparasitic mite Varroa destructor uses chemical mimicry to access and remain undetected inside colonies of its honey bee host, Apis mellifera. It remains, however, to be tested whether the chemical profile of V. destructor mirrors colony-specific cues of the host’s chemistry that allows con-specific nest-mate discrimination to occur in honey bees. Here we show that colony-specific differences in the chemical profile of four A. mellifera colonies were based on differences in the n-alkane:alkene ratio. These colony-specific differences in chemical profile were mirrored by V. destructor mites collected from the same four colonies, even though overall chemical mimicry was imperfect

The rogue nature of hiatuses in a global warming climate

The nature of rogue events is their unlikelihood and the recent unpredicted decade-long slowdown in surface warming, the so-called hiatus, may be such an event. However, given decadal variability in climate, global surface temperatures were never expected to increase monotonically with increasing radiative forcing. Here surface air temperature from 20 climate models is analyzed to estimate the historical and future likelihood of hiatuses and “surges” (faster than expected warming), showing that the global hiatus of the early 21st century was extremely unlikely. A novel analysis of future climate scenarios suggests that hiatuses will almost vanish and surges will strongly intensify by 2100 under a “business as usual” scenario. For “CO2 stabilisation” scenarios, hiatus, and surge characteristics revert to typical 1940s values. These results suggest to study the hiatus of the early 21st century and future reoccurrences as rogue events, at the limit of the variability of current climate modelling capability

Jets and Topography: Jet Transitions and the Impact on Transport in the Antarctic Circumpolar Current

The Southern Ocean’s Antarctic Circumpolar Current (ACC) naturally lends itself to interpretations using a zonally averaged framework. Yet, navigation around steep and complicated bathymetric obstacles suggests that local dynamics may be far removed from those described by zonally symmetric models. In this study, both observational and numerical results indicate that zonal asymmetries, in the form of topography, impact global flow structure and transport properties. The conclusions are based on a suite of more than 1.5 million virtual drifter trajectories advected using a satellite altimetry–derived surface velocity field spanning 17 years. The focus is on sites of “cross front” transport as defined by movement across selected sea surface height contours that correspond to jets along most of the ACC. Cross-front exchange is localized in the lee of bathymetric features with more than 75% of crossing events occurring in regions corresponding to only 20% of the ACC’s zonal extent. These observations motivate a series of numerical experiments using a two-layer quasigeostrophic model with simple, zonally asymmetric topography, which often produces transitions in the front structure along the channel. Significantly, regimes occur where the equilibrated number of coherent jets is a function of longitude and transport barriers are not periodic. Jet reorganization is carried out by eddy flux divergences acting to both accelerate and decelerate the mean flow of the jets. Eddy kinetic energy is amplified downstream of topography due to increased baroclinicity related to topographic steering. The combination of high eddy kinetic energy and recirculation features enhances particle exchange. These results stress the complications in developing consistent circumpolar definitions of the ACC fronts

Effect of rearing temperature on physiological measures and antioxidant status of broiler chickens fed stevia (Stevia rebaudiana B.) leaf meal and exogenous xylanase

Background The global climate is warming. Heat stress, as a result of high ambient temperatures, may negatively impact physiology and reduce growth performance of poultry. Stevia is a perennial shrub indigenous to South America where its phytochemical extracts have been used as a natural sweetener for hundreds of years. Its physiological effects, including antioxidant properties, on poultry are well known, however, the translation of these to improved growth performance is variable. Combining stevia with a commercial xylanase to enhance feed digestibility could therefore form a feeding strategy to partially mitigate the negative impact of rearing birds under high ambient temperatures. Purpose The study aimed to compare the growth performance, dietary energy and nutrient availability, oxidative status, gastrointestinal tract development, and caecal short chain fatty acid concentration; at two ambient rearing temperatures, when feeding diets containing stevia and exogenous xylanase, alone or in combination, to broiler chickens. Study design/Methods: Day-old chicks (n = 105) were reared in a single floor pen following breeder recommendations for the first 7 days, whereupon birds (n = 96) were randomly allocated to one of four experimental diets (negative control, stevia at 20 g/kg diet, xylanase at 100 FXU/kg diet, stevia at 20 g/kg diet + xylanase at 100 FXU/kg diet), in one of two environmental conditions (high ambient temperature at 32 ± 2 °C or regular rearing at breeder recommendations), in a 2 × 2 × 2 factorial design. Results Rearing birds at high ambient temperature reduced daily feed intake (p = 0.02). Birds fed stevia and reared at regular temperature had similar weight gain to birds reared in high ambient temperatures, although birds on the control diet housed at regular temperatures had the greatest weight gain (P < 0.05). Exogenous xylanase improved overall dietary metabolisable energy and improved nitrogen retention in the high ambient temperature group only (P < 0.05). Dietary stevia reduced caecal digesta butyric acid: acetic acid at regular temperature, but xylanase increased the butyric acid concentration at high ambient temperature (P < 0.05). Dietary stevia increased (P < 0.001) the hepatic carotenoid concentrations and xylanase improved (P < 0.05) hepatic vitamin E concentrations. Conclusions Rearing temperature is an important environmental factor in broiler production. Exogenous xylanase supplementation can increase feed efficiency and dietary metabolisable energy. Feeding xylanase or stevia improves hepatic antioxidant status in broilers by increasing hepatic vitamin E and carotenoids, respectively, suggesting that either may be effective in counteracting oxidative stress

Exploiting thrips aggregation pheromones to develop a lure-and-kill strategy for the management of the bean flower thrips

The potential of semiochemicals to lure insect pests to a trap where they can be killed with biopesticides has been demonstrated as an eco-friendly pest management alternative. In this study, we tested two recently characterized male-produced aggregation pheromones of the bean flower thrips Megalurothrips sjostedti (Trybom), namely (R)-lavandulyl 3-methylbutanoate (major) and (R)-lavandulol (minor), for their field efficacy. Moreover, compatibility of these pheromones and two other thrips attractants, Lurem-TR and neryl (S)-2-methylbutanoate, with the entomopathogenic fungus (EPF) Metarhizium anisopliae ICIPE 69 has been determined. Our study revealed that the M. sjostedti aggregation pheromones have dose-dependent antifungal effects on the EPF viability, but showed no fungistatic effect at a field-realistic dose for attraction of thrips. (R)-lavandulyl 3-methylbutanoate had similar antifungal effects as neryl (S)-2-methylbutanoate 8 days after exposure; whereas, Lurem-TR had a stronger antifungal effect than other thrips attractants. In the semi-field experiments, all autoinoculation devices maintained at least 86% viability of M. anisopliae conidia after 12 days of exposure. Field trials demonstrated for the first time that (R)-lavandulyl 3-methylbutanoate increases trap catches. Our findings pave a way for designing a lure-and-kill thrips management strategy to control bean flower thrips using autoinoculation devices or spot spray application