The effect of on/off indicator design on state confusion, preference, and response time performance, executive summary

Investigated are five designs of software-based ON/OFF indicators in a hypothetical Space Station Power System monitoring task. The hardware equivalent of the indicators used in the present study is the traditional indicator light that illuminates an ON label or an OFF label. Coding methods used to represent the active state were reverse video, color, frame, check, or reverse video with check. Display background color was also varied. Subjects made judgments concerning the state of indicators that resulted in very low error rates and high percentages of agreement across indicator designs. Response time measures for each of the five indicator designs did not differ significantly, although subjects reported that color was the best communicator. The impact of these results on indicator design is discussed

Quasiperiodic graphs: structural design, scaling and entropic properties

A novel class of graphs, here named quasiperiodic, are constructed via application of the Horizontal Visibility algorithm to the time series generated along the quasiperiodic route to chaos. We show how the hierarchy of mode-locked regions represented by the Farey tree is inherited by their associated graphs. We are able to establish, via Renormalization Group (RG) theory, the architecture of the quasiperiodic graphs produced by irrational winding numbers with pure periodic continued fraction. And finally, we demonstrate that the RG fixed-point degree distributions are recovered via optimization of a suitably defined graph entropy

Host-selected mutations converging on a global regulator drive an adaptive leap towards symbiosis in bacteria

Host immune and physical barriers protect against pathogens but also impede the establishment of essential symbiotic partnerships. To reveal mechanisms by which beneficial organisms adapt to circumvent host defenses, we experimentally evolved ecologically distinct bioluminescent Vibrio fischeri by colonization and growth within the light organs of the squid Euprymna scolopes. Serial squid passaging of bacteria produced eight distinct mutations in the binK sensor kinase gene, which conferred an exceptional selective advantage that could be demonstrated through both empirical and theoretical analysis. Squid-adaptive binK alleles promoted colonization and immune evasion that were mediated by cell-associated matrices including symbiotic polysaccharide (Syp) and cellulose. binK variation also altered quorum sensing, raising the threshold for luminescence induction. Preexisting coordinated regulation of symbiosis traits by BinK presented an efficient solution where altered BinK function was the key to unlock multiple colonization barriers. These results identify a genetic basis for microbial adaptability and underscore the importance of hosts as selective agents that shape emergent symbiont populations

Presence and fate of priority substances in domestic greywater treatment and reuse systems

A wide range of household sources may potentially contribute to contaminant loads in domestic greywater. The ability of greywater treatment systems to act as emission control barriers for household micropollutants, thereby providing environmental benefits in addition to potable water savings, have not been fully explored. This paper investigates the sources, presence and potential fate of a selection of xenobiotic micropollutants in on-site greywater treatment systems. All of the investigated compounds are listed under the European Water Framework Directive as either "Priority Substances" (PS) or "Priority Hazardous Substances" (PHS). Significant knowledge gaps are identified. A wide range of potential treatment trains are available for greywater treatment and reuse but treatment efficiency data for priority substances and other micropollutants is very limited. Geochemical modelling indicates that PS/PHS removal during treatment is likely to be predominantly due to sludge/solid phase adsorption, with only minor contributions to the water phase. Many PS/PHS are resistant to biodegradation and as the majority of automated greywater treatment plants periodically discharge sludge to the municipal sewerage system, greywater treatment is unlikely to act as a comprehensive PS/PHS emission barrier. Hence, it is important to ensure that other source control options (e.g. eco-labeling, substance substitution, and regulatory controls) for household items continue to be pursued, in order that PS/PHS emissions from these sources are effectively reduced and/or phased out as required under the demands of the European Water Framework Directive

Sample size calculations for cluster randomised controlled trials with a fixed number of clusters

Background\ud Cluster randomised controlled trials (CRCTs) are frequently used in health service evaluation. Assuming an average cluster size, required sample sizes are readily computed for both binary and continuous outcomes, by estimating a design effect or inflation factor. However, where the number of clusters are fixed in advance, but where it is possible to increase the number of individuals within each cluster, as is frequently the case in health service evaluation, sample size formulae have been less well studied. \ud \ud Methods\ud We systematically outline sample size formulae (including required number of randomisation units, detectable difference and power) for CRCTs with a fixed number of clusters, to provide a concise summary for both binary and continuous outcomes. Extensions to the case of unequal cluster sizes are provided. \ud \ud Results\ud For trials with a fixed number of equal sized clusters (k), the trial will be feasible provided the number of clusters is greater than the product of the number of individuals required under individual randomisation ($n_i$) and the estimated intra-cluster correlation ($\rho$). So, a simple rule is that the number of clusters ($\kappa$) will be sufficient provided: \ud \ud $\kappa$ > $n_i$ x $\rho$\ud \ud Where this is not the case, investigators can determine the maximum available power to detect the pre-specified difference, or the minimum detectable difference under the pre-specified value for power. \ud \ud Conclusions\ud Designing a CRCT with a fixed number of clusters might mean that the study will not be feasible, leading to the notion of a minimum detectable difference (or a maximum achievable power), irrespective of how many individuals are included within each cluster. \ud \u

A Strategy for Detecting Natural Anthelmintic Constituents of the Grassland Species \u3ci\u3ePlantago lanceolata\u3c/i\u3e

A strategy to detect anthelmintic constituents in plantain (Plantago lanceolata) using a bioassay-driven purification approach was tested. Plantain consumed by cattle may control or reduce internal parasite titers, possibly due to the iridoid glucoside aucubin. Lyophilized, ground leaves of wild P. lanceolata were extracted with 95 % ethanol or boiling water containing calcium carbonate. Partially purified extracts (0 to 250 mg ml-1), 5 μg ml-1 of the anthelmintic levamisole, or 5 mg ml-1 of aucubin were tested with sheathed bovine parasites (Ostertagia ostertagi). The percent moving worms was unchanged for water controls and reduced to 0 % for anthelmintic levamisole. Aucubin significantly reduced the number of swimming worms at day 2, but they returned to starting values at day 3. Extracts from tall fescue and white clover foliage did not show anthelmintic effects. Treatment of extract or aucubin with ß-glucosidase did not alter their activity. Beneficial anthelmintic action of ingested plantain is not due to aucubin

Ecology and Genetic Structure of a Northern Temperate Vibrio cholerae Population Related to Toxigenic Isolates

Although Vibrio cholerae is an important human pathogen, little is known about its populations in regions where the organism is endemic but where cholera disease is rare. A total of 31 independent isolates confirmed as V. cholerae were collected from water, sediment, and oysters in 2008 and 2009 from the Great Bay Estuary (GBE) in New Hampshire, a location where the organism has never been detected. Environmental analyses suggested that abundance correlates most strongly with rainfall events, as determined from data averaged over several days prior to collection. Phenotyping, genotyping, and multilocus sequence analysis (MLSA) revealed a highly diverse endemic population, with clones recurring in both years. Certain isolates were closely related to toxigenic O1 strains, yet no virulence genes were detected. Multiple statistical tests revealed evidence of recombination among strains that contributed to allelic diversity equally as mutation. This relatively isolated population discovered on the northern limit of detection for V. cholerae can serve as a model of natural population dynamics that augments predictive models for disease emergence

Time interval distributions of atoms in atomic beams

We report on the experimental investigation of two-particle correlations between neutral atoms in a Hanbury Brown and Twiss experiment. Both an atom laser beam and a pseudo-thermal atomic beam are extracted from a Bose-Einstein condensate and the atom flux is measured with a single atom counter. We determine the conditional and the unconditional detection probabilities for the atoms in the beam and find good agreement with the theoretical predictions.Comment: 4 pages, 3 figure

The dual rod system of amphibians supports colour discrimination at the absolute visual threshold

The presence of two spectrally different kinds of rod photoreceptors in amphibians has been hypothesized to enable purely rod-based colour vision at very low light levels. The hypothesis has never been properly tested, so we performed three behavioural experiments at different light intensities with toads (Bufo) and frogs (Rana) to determine the thresholds for colour discrimination. The thresholds of toads were different in mate choice and prey-catching tasks, suggesting that the differential sensitivities of different spectral cone types as well as task-specific factors set limits for the use of colour in these behavioural contexts. In neither task was there any indication of rod-based colour discrimination. By contrast, frogs performing phototactic jumping were able to distinguish blue from green light down to the absolute visual threshold, where vision relies only on rod signals. The remarkable sensitivity of this mechanism comparing signals from the two spectrally different rod types approaches theoretical limits set by photon fluctuations and intrinsic noise. Together, the results indicate that different pathways are involved in processing colour cues depending on the ecological relevance of this information for each task. This article is part of the themed issue 'Vision in dim light'.Peer reviewe